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The map label for this gene is hipO [H]

Identifier: 146339913

GI number: 146339913

Start: 3117173

End: 3118336

Strand: Reverse

Name: hipO [H]

Synonym: BRADO2917

Alternate gene names: 146339913

Gene position: 3118336-3117173 (Counterclockwise)

Preceding gene: 146339914

Following gene: 146339912

Centisome position: 41.82

GC content: 66.75

Gene sequence:

>1164_bases
ATGCCCACGATCGACCGTATCCAAGGCTACGCCGACGAGCTCACCGCCATCCGCCGCGACCTGCACGCGCATCCCGAGAT
CGGCTTCGAGGAGACGCGCACCTCCGGCATCGTCGCCGAAAAGCTCGCTCAATGGGGCATCGAGGTGCATCGCGGCCTCG
GCGGCACCGGCGTGATCGGCGTGCTCAAGGGCAAGGGCGACAGCGGCAAGACAATCGGCCTGCGCGCCGACATGGACGCG
CTGCCGATGGAGGAGAACACCAATCTGCCCTGGCGCTCGACCATTCCCGGCCGCTTCCACGGCTGCGGCCATGACGGCCA
CACCACGATGCTGCTTGGCACCGCGCGCTATCTCGCCGAGACGCGCAATTTCGACGGCACCGTGCATTTCATCTTCCAGC
CGGCCGAGGAAGGCCTCGGCGGCGCGCGCGCGATGATCAAGGACGGGCTGTTCCAGAAATTCCCCTGCGACGAACTCTAC
GGCCTGCACAACGCGCCGGATCTCGAACACGGCGAGATCGCGATCCTGCCCGGCCCGGCAATGGCCGGCGCCGACTTCTT
CGATATCAGAATCTCCGGCTATGGCGCCCATGGCGCGATGCCCGAGCGCTCCAAGGACGCAGTTGTGATCGCGACCAGCG
TCGCGCAGGCGATCCAGACCATCGTCAGCCGCAACGTCGAGCCGCTGCAGGCCGCCGTGGTCTCGATCACGCAGATCCAT
GCCGGCTCCGCCTACAACGTCATTCCGGGCGACGCCTGGCTCTGCGGCACCGTGCGCGCGTTCTCCGACAATGTCCGCGC
GCTGGTGCGCGAGCGCATGCGCGCGATCTGCGCCGGCGTGGCAGCCGCCTTCAACTGCGAGATCGACGTCGACATCCGCG
ACACGTTCAGCGTCCTGGTCAACCAGGAGGAGCAGTCCAAGGTCGTCGAGGAGGTCGCGCGCACCGTCGTCGAGCCGTCC
AAGGTCATCACGCGCTCGACGCCGAAGATGGGCAGCGAGGATTTCGCCGACATGCTGCAGACCATCCCGGGCGCCTATTT
CTGGGTCGGCCATGATGGCTCGGTGCCGGTGCACAACCCCGGCTACGTGCTCGACGACAAGATCCTGCCGATCGGCGCGA
GCATGTTCGCAAGGATCATCGAGACCCGTCTGGCGGCGGCCTAA

Upstream 100 bases:

>100_bases
TGGCGGGCGTCTCGAAGGATGGGCCGCAAGCTCGGTCGCAGACCAGTCTCTTTGCACCGCCTCCTGACTGCTCCCTTTTC
ACTCAACGGACACGCAACCC

Downstream 100 bases:

>100_bases
CGCCGCCTGACGTATGTCAATGAGCGGGCCTGGAATTGAGACGAACTTCGGCGACGGAGGACCAGCATGCACAAGCCCGT
TTCAGACGACGTCACCTCGT

Product: putative hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPTIDRIQGYADELTAIRRDLHAHPEIGFEETRTSGIVAEKLAQWGIEVHRGLGGTGVIGVLKGKGDSGKTIGLRADMDA
LPMEENTNLPWRSTIPGRFHGCGHDGHTTMLLGTARYLAETRNFDGTVHFIFQPAEEGLGGARAMIKDGLFQKFPCDELY
GLHNAPDLEHGEIAILPGPAMAGADFFDIRISGYGAHGAMPERSKDAVVIATSVAQAIQTIVSRNVEPLQAAVVSITQIH
AGSAYNVIPGDAWLCGTVRAFSDNVRALVRERMRAICAGVAAAFNCEIDVDIRDTFSVLVNQEEQSKVVEEVARTVVEPS
KVITRSTPKMGSEDFADMLQTIPGAYFWVGHDGSVPVHNPGYVLDDKILPIGASMFARIIETRLAAA

Sequences:

>Translated_387_residues
MPTIDRIQGYADELTAIRRDLHAHPEIGFEETRTSGIVAEKLAQWGIEVHRGLGGTGVIGVLKGKGDSGKTIGLRADMDA
LPMEENTNLPWRSTIPGRFHGCGHDGHTTMLLGTARYLAETRNFDGTVHFIFQPAEEGLGGARAMIKDGLFQKFPCDELY
GLHNAPDLEHGEIAILPGPAMAGADFFDIRISGYGAHGAMPERSKDAVVIATSVAQAIQTIVSRNVEPLQAAVVSITQIH
AGSAYNVIPGDAWLCGTVRAFSDNVRALVRERMRAICAGVAAAFNCEIDVDIRDTFSVLVNQEEQSKVVEEVARTVVEPS
KVITRSTPKMGSEDFADMLQTIPGAYFWVGHDGSVPVHNPGYVLDDKILPIGASMFARIIETRLAAA
>Mature_386_residues
PTIDRIQGYADELTAIRRDLHAHPEIGFEETRTSGIVAEKLAQWGIEVHRGLGGTGVIGVLKGKGDSGKTIGLRADMDAL
PMEENTNLPWRSTIPGRFHGCGHDGHTTMLLGTARYLAETRNFDGTVHFIFQPAEEGLGGARAMIKDGLFQKFPCDELYG
LHNAPDLEHGEIAILPGPAMAGADFFDIRISGYGAHGAMPERSKDAVVIATSVAQAIQTIVSRNVEPLQAAVVSITQIHA
GSAYNVIPGDAWLCGTVRAFSDNVRALVRERMRAICAGVAAAFNCEIDVDIRDTFSVLVNQEEQSKVVEEVARTVVEPSK
VITRSTPKMGSEDFADMLQTIPGAYFWVGHDGSVPVHNPGYVLDDKILPIGASMFARIIETRLAAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=425, Percent_Identity=27.0588235294118, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41741; Mature: 41610

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTIDRIQGYADELTAIRRDLHAHPEIGFEETRTSGIVAEKLAQWGIEVHRGLGGTGVIG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEE
VLKGKGDSGKTIGLRADMDALPMEENTNLPWRSTIPGRFHGCGHDGHTTMLLGTARYLAE
EEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
TRNFDGTVHFIFQPAEEGLGGARAMIKDGLFQKFPCDELYGLHNAPDLEHGEIAILPGPA
HCCCCCEEEEEEECCHHCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCC
MAGADFFDIRISGYGAHGAMPERSKDAVVIATSVAQAIQTIVSRNVEPLQAAVVSITQIH
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHEE
AGSAYNVIPGDAWLCGTVRAFSDNVRALVRERMRAICAGVAAAFNCEIDVDIRDTFSVLV
CCCCCEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEHHHHHHHHH
NQEEQSKVVEEVARTVVEPSKVITRSTPKMGSEDFADMLQTIPGAYFWVGHDGSVPVHNP
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
GYVLDDKILPIGASMFARIIETRLAAA
CEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTIDRIQGYADELTAIRRDLHAHPEIGFEETRTSGIVAEKLAQWGIEVHRGLGGTGVIG
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEE
VLKGKGDSGKTIGLRADMDALPMEENTNLPWRSTIPGRFHGCGHDGHTTMLLGTARYLAE
EEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
TRNFDGTVHFIFQPAEEGLGGARAMIKDGLFQKFPCDELYGLHNAPDLEHGEIAILPGPA
HCCCCCEEEEEEECCHHCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCC
MAGADFFDIRISGYGAHGAMPERSKDAVVIATSVAQAIQTIVSRNVEPLQAAVVSITQIH
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHEE
AGSAYNVIPGDAWLCGTVRAFSDNVRALVRERMRAICAGVAAAFNCEIDVDIRDTFSVLV
CCCCCEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEHHHHHHHHH
NQEEQSKVVEEVARTVVEPSKVITRSTPKMGSEDFADMLQTIPGAYFWVGHDGSVPVHNP
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
GYVLDDKILPIGASMFARIIETRLAAA
CEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]