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The map label for this gene is melA [H]

Identifier: 146339865

GI number: 146339865

Start: 3066958

End: 3068238

Strand: Direct

Name: melA [H]

Synonym: BRADO2867

Alternate gene names: 146339865

Gene position: 3066958-3068238 (Clockwise)

Preceding gene: 146339864

Following gene: 146339866

Centisome position: 41.13

GC content: 66.04

Gene sequence:

>1281_bases
ATGACCGCCGTCACCAAGATCGTCTTCCTCGGTGCGAGCAGCGCATCGTTTGGGCTCAGCGTGTTTCGGGACTTGTTCTC
GACCCAGGATCTCGCGGGATCGACGCTGGTTCTGGTCGGTCGGGATACCACGCGGCTCGCGCGGTCGGAACGGCTGGCCC
GGCTGCTGAACGACCGCTCCGGTGCGGGCCTCAGGATCGAGGCGACGACGGATTGGCGCGCCGCGCTCGACGGCGCCGGT
TTCGTGATTCATTCCACCGCGATCGACCGCAATCGGCTGTGGAAGCTCGACTTCGAAGTGCCGCGCAAATACGGTATCCG
CCATACGCTCGGTGAGAATGGCGGGCCGGGCGCGTTGTTCTTTACGCTCCGCACCTTGCCTGTTGTGTTCGACTTCGTTC
GGGAAATGGAGCAGCGCTGCCCGAACGCGCTGTTCATCAACTTCTCCAATCCCGAGAGCCGCATCATCCTGGCACTCGGT
CTTCACAGCCGGCTCGCCCATGTCGGCCTGTGCCACGGCATCTTCATCGCGCGGAGCCAAGTGGCGTCGATCCTCGGGCT
GGCGGAGGAGCGCGTCGAGGTATGGGGCGCCGGCCTCAATCATTTCCAGTGCCTGCTGCAGATCCGTGACCGTGAAACCG
GAGCCGACCTCTATCCGCTGCTGCGCGAACGGGAGCGCAGTCACGACCCCAAGGTCGCGCCGCTGACCCGCACGCTGCTG
CATGATTTCGGCGTCTGGCTCGGCTGCGGCGATGGCCATGTCGGCGAGTATCTCGCTTATGGCTGGGAGGCGGGCGAGCG
CGGCTATGATTTTGCCTGGGATGAGGCGGAACGCGCCAGGCTGATCCGCCAGATCGACGACGTGCTGTCGGGAACGGCCG
AGATGCCGGCTTGGTGGCGGTCCCCGTCGGGCGAACGCGCGGTGACCGTGATCGGTGCGGTGCTGCACGATCGCAAGCAG
TCCATCGAGTCCGGCGTCGTGCTCAACCGCGGCGTCATCCCCAATCTCCCGGCCGAGGCGGCCGTCGAAGTGCCTGTCGT
TGCGGATCGCTGCGGCATTCATCCGATCTCCTTGGGTCCGCTGCCGAACGCCGTCGCCAAGCTGATGGCGATCCAGGTTC
AGGTGCAGCAGCTCTCCGTGCAGGCGGCGATGACCGCCTCCAGGGACGTCGCGCTGCAGGCGCTGCTGGTCGACCCGGTC
GTGAACTCGGCCACGGCGGCTCGCCAGATCCTCGACGAACTCTGGGAGGTGAACCGGCCCTACATTCGGCCATGCGCCTG
A

Upstream 100 bases:

>100_bases
TCATCGAAGGCACGTGTTCAAGGCGGCCGCCTCGCATCGGCGTGGCGGCCGTCTTGATCTGGATCAGGATGACGGCGCAG
CGTCATGGCACAGATGTCGG

Downstream 100 bases:

>100_bases
TTGGGTGAGCGCAAGGCGCGGTAATCCGCACGCACCGCTCCGAAGCGCCGGCGGTTCCTATCAGACGGCGAAAGCCCTTT
ACAATGACTGACTGGTCAGT

Product: putative alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTAVTKIVFLGASSASFGLSVFRDLFSTQDLAGSTLVLVGRDTTRLARSERLARLLNDRSGAGLRIEATTDWRAALDGAG
FVIHSTAIDRNRLWKLDFEVPRKYGIRHTLGENGGPGALFFTLRTLPVVFDFVREMEQRCPNALFINFSNPESRIILALG
LHSRLAHVGLCHGIFIARSQVASILGLAEERVEVWGAGLNHFQCLLQIRDRETGADLYPLLRERERSHDPKVAPLTRTLL
HDFGVWLGCGDGHVGEYLAYGWEAGERGYDFAWDEAERARLIRQIDDVLSGTAEMPAWWRSPSGERAVTVIGAVLHDRKQ
SIESGVVLNRGVIPNLPAEAAVEVPVVADRCGIHPISLGPLPNAVAKLMAIQVQVQQLSVQAAMTASRDVALQALLVDPV
VNSATAARQILDELWEVNRPYIRPCA

Sequences:

>Translated_426_residues
MTAVTKIVFLGASSASFGLSVFRDLFSTQDLAGSTLVLVGRDTTRLARSERLARLLNDRSGAGLRIEATTDWRAALDGAG
FVIHSTAIDRNRLWKLDFEVPRKYGIRHTLGENGGPGALFFTLRTLPVVFDFVREMEQRCPNALFINFSNPESRIILALG
LHSRLAHVGLCHGIFIARSQVASILGLAEERVEVWGAGLNHFQCLLQIRDRETGADLYPLLRERERSHDPKVAPLTRTLL
HDFGVWLGCGDGHVGEYLAYGWEAGERGYDFAWDEAERARLIRQIDDVLSGTAEMPAWWRSPSGERAVTVIGAVLHDRKQ
SIESGVVLNRGVIPNLPAEAAVEVPVVADRCGIHPISLGPLPNAVAKLMAIQVQVQQLSVQAAMTASRDVALQALLVDPV
VNSATAARQILDELWEVNRPYIRPCA
>Mature_425_residues
TAVTKIVFLGASSASFGLSVFRDLFSTQDLAGSTLVLVGRDTTRLARSERLARLLNDRSGAGLRIEATTDWRAALDGAGF
VIHSTAIDRNRLWKLDFEVPRKYGIRHTLGENGGPGALFFTLRTLPVVFDFVREMEQRCPNALFINFSNPESRIILALGL
HSRLAHVGLCHGIFIARSQVASILGLAEERVEVWGAGLNHFQCLLQIRDRETGADLYPLLRERERSHDPKVAPLTRTLLH
DFGVWLGCGDGHVGEYLAYGWEAGERGYDFAWDEAERARLIRQIDDVLSGTAEMPAWWRSPSGERAVTVIGAVLHDRKQS
IESGVVLNRGVIPNLPAEAAVEVPVVADRCGIHPISLGPLPNAVAKLMAIQVQVQQLSVQAAMTASRDVALQALLVDPVV
NSATAARQILDELWEVNRPYIRPCA

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=438, Percent_Identity=29.2237442922374, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788029, Length=392, Percent_Identity=25.765306122449, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 46832; Mature: 46701

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVTKIVFLGASSASFGLSVFRDLFSTQDLAGSTLVLVGRDTTRLARSERLARLLNDRS
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCC
GAGLRIEATTDWRAALDGAGFVIHSTAIDRNRLWKLDFEVPRKYGIRHTLGENGGPGALF
CCCEEEEECCCHHHHHCCCCEEEEEEECCCCCEEEEECCCCHHCCCHHHCCCCCCCCEEE
FTLRTLPVVFDFVREMEQRCPNALFINFSNPESRIILALGLHSRLAHVGLCHGIFIARSQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
VASILGLAEERVEVWGAGLNHFQCLLQIRDRETGADLYPLLRERERSHDPKVAPLTRTLL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
HDFGVWLGCGDGHVGEYLAYGWEAGERGYDFAWDEAERARLIRQIDDVLSGTAEMPAWWR
HHCCCEEECCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHC
SPSGERAVTVIGAVLHDRKQSIESGVVLNRGVIPNLPAEAAVEVPVVADRCGIHPISLGP
CCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEECCEEHHHCCCCEEECCC
LPNAVAKLMAIQVQVQQLSVQAAMTASRDVALQALLVDPVVNSATAARQILDELWEVNRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YIRPCA
CCCCCC
>Mature Secondary Structure 
TAVTKIVFLGASSASFGLSVFRDLFSTQDLAGSTLVLVGRDTTRLARSERLARLLNDRS
CCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCC
GAGLRIEATTDWRAALDGAGFVIHSTAIDRNRLWKLDFEVPRKYGIRHTLGENGGPGALF
CCCEEEEECCCHHHHHCCCCEEEEEEECCCCCEEEEECCCCHHCCCHHHCCCCCCCCEEE
FTLRTLPVVFDFVREMEQRCPNALFINFSNPESRIILALGLHSRLAHVGLCHGIFIARSQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
VASILGLAEERVEVWGAGLNHFQCLLQIRDRETGADLYPLLRERERSHDPKVAPLTRTLL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
HDFGVWLGCGDGHVGEYLAYGWEAGERGYDFAWDEAERARLIRQIDDVLSGTAEMPAWWR
HHCCCEEECCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHC
SPSGERAVTVIGAVLHDRKQSIESGVVLNRGVIPNLPAEAAVEVPVVADRCGIHPISLGP
CCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEECCEEHHHCCCCEEECCC
LPNAVAKLMAIQVQVQQLSVQAAMTASRDVALQALLVDPVVNSATAARQILDELWEVNRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YIRPCA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]