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The map label for this gene is kgtP [H]

Identifier: 146339540

GI number: 146339540

Start: 2703405

End: 2704748

Strand: Reverse

Name: kgtP [H]

Synonym: BRADO2531

Alternate gene names: 146339540

Gene position: 2704748-2703405 (Counterclockwise)

Preceding gene: 146339541

Following gene: 146339539

Centisome position: 36.27

GC content: 64.29

Gene sequence:

>1344_bases
GTGCCCCGGAGACCGGACATGACAGACCAGGCGATCGAGCCTGCCGTAGAGCCCGCCACGTCGATCGCGGACGTCGAGCG
CCGCATCAAGGCGATCCTGATCGGCTCCGCCGGCAACCTCGTCGAATGGTACGATTTCTATGCCTACACCGCGTTCGCGC
TGTATTTCGCGCCGGCGTTCTTCCCCTCGAAGGATCCGGTCGTCGAGCAGCTCAACGCCGCGGTGGTGTTCGCCGCCACC
TTCCTGATGCGCCCGCTCGGCGGCTGGCTGTTCGGCTATCTCGCCGACCAGTATGGCCGGCGGCTGTCGCTCACGATCTC
CGTACTCTGCATGTGCTTCGGCTCGCTGATCATCGCGGTGACGCCGACCTATGCCACGATCGGCATCGCGGCGCCGATCA
TCCTGGCGCTCGCCCGCGTGATCGAGGGGCTGAGCCTCGGCGGCGAATATGGCGCCAGCGCCACCTATCTGTCGGAAGTC
GCCGACGCCAAGCACCGCGGCTTCTATTCCAGCTTCCAATACGTCACTTTGATCGGCGGCCAGCTCACCGCGATCATCGT
GCTCCTGCTGCTGCAGAAGGTGTTTCTGACCACGGACGAGCTGAAGGCCTGGGGCTGGCGCATCCCGTTCTTCATCGGCG
CCTGTCTTGCGATCTTCGTCGCGGTGATGCGGCGCAATCTGCACGAGACCGAGGCCTTCGTGGAGGCCAAGAAGAAGGCG
GGCTCGTCCAGCTCGATGGCCGCGCTGTTCCGATATCCGCGCGAGCTGCTGCTCGTCGTCGGCCTCACCGCCGGCGGCAC
CGCGGCATTCTATACATTCACGACCTACATGCAGACCTTCGTGAAACTCTCGGTCGGCCTGACCGAGGACCAGACCACGA
TGGTGATCTTCGGCTCGCTGCTGTTCGCCTGCGTGCTGCAGCCGCTGTACGGCGCGATCTCCGACCGCATCGGCCGCAAG
CCGCTCTTGATCTTCTTCGGCATCGCCGGCACGCTGTCGACGATCCCGATCCTGACCGCGCTCAAGGCCACCAAGGAGCC
GTTCACGGCCTTCCTGCTGATCTGCGGCGCCTGGATCTTCACCGCCGGCTACACCTCGATCAATGCCGTGGTCAAAGCCG
AGCTGTTCCCGACCAACATCCGCGCGCTGGGCGTCGGCCTGCCCTACGCGATCACCGTGTCGCTGTTCGGCGGCACCGCG
CCGGCGATCGCGCTCTATTTCAAGAGCATCGGGCACGAAACCTGGTTCTATTATTACCTGTCCGGCGTGATCTTCCTCTC
GCTGCTCATCTATGCGACCATGCGCGACACCAAGCATGCGTCCGCCATGCACAGGCATGAGTGA

Upstream 100 bases:

>100_bases
TCCAGAGCCGGCGAGTTCCTCCCGAAGGAACATACGACCCCCGCCCTTATTCCATATTGGCAATCCCGGCGGCTCCCGTA
GAAGGGCCGCAAAATCACGC

Downstream 100 bases:

>100_bases
GCCGCTCAAATCGACGGGGTGCCGAGATGGCAGACGAGTCCGGGCTGCCGCCCGAGAGCAAGCTGACCCGCAGCAAGGAA
CGCTGGGCGCGCGAGGGCAA

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPRRPDMTDQAIEPAVEPATSIADVERRIKAILIGSAGNLVEWYDFYAYTAFALYFAPAFFPSKDPVVEQLNAAVVFAAT
FLMRPLGGWLFGYLADQYGRRLSLTISVLCMCFGSLIIAVTPTYATIGIAAPIILALARVIEGLSLGGEYGASATYLSEV
ADAKHRGFYSSFQYVTLIGGQLTAIIVLLLLQKVFLTTDELKAWGWRIPFFIGACLAIFVAVMRRNLHETEAFVEAKKKA
GSSSSMAALFRYPRELLLVVGLTAGGTAAFYTFTTYMQTFVKLSVGLTEDQTTMVIFGSLLFACVLQPLYGAISDRIGRK
PLLIFFGIAGTLSTIPILTALKATKEPFTAFLLICGAWIFTAGYTSINAVVKAELFPTNIRALGVGLPYAITVSLFGGTA
PAIALYFKSIGHETWFYYYLSGVIFLSLLIYATMRDTKHASAMHRHE

Sequences:

>Translated_447_residues
MPRRPDMTDQAIEPAVEPATSIADVERRIKAILIGSAGNLVEWYDFYAYTAFALYFAPAFFPSKDPVVEQLNAAVVFAAT
FLMRPLGGWLFGYLADQYGRRLSLTISVLCMCFGSLIIAVTPTYATIGIAAPIILALARVIEGLSLGGEYGASATYLSEV
ADAKHRGFYSSFQYVTLIGGQLTAIIVLLLLQKVFLTTDELKAWGWRIPFFIGACLAIFVAVMRRNLHETEAFVEAKKKA
GSSSSMAALFRYPRELLLVVGLTAGGTAAFYTFTTYMQTFVKLSVGLTEDQTTMVIFGSLLFACVLQPLYGAISDRIGRK
PLLIFFGIAGTLSTIPILTALKATKEPFTAFLLICGAWIFTAGYTSINAVVKAELFPTNIRALGVGLPYAITVSLFGGTA
PAIALYFKSIGHETWFYYYLSGVIFLSLLIYATMRDTKHASAMHRHE
>Mature_446_residues
PRRPDMTDQAIEPAVEPATSIADVERRIKAILIGSAGNLVEWYDFYAYTAFALYFAPAFFPSKDPVVEQLNAAVVFAATF
LMRPLGGWLFGYLADQYGRRLSLTISVLCMCFGSLIIAVTPTYATIGIAAPIILALARVIEGLSLGGEYGASATYLSEVA
DAKHRGFYSSFQYVTLIGGQLTAIIVLLLLQKVFLTTDELKAWGWRIPFFIGACLAIFVAVMRRNLHETEAFVEAKKKAG
SSSSMAALFRYPRELLLVVGLTAGGTAAFYTFTTYMQTFVKLSVGLTEDQTTMVIFGSLLFACVLQPLYGAISDRIGRKP
LLIFFGIAGTLSTIPILTALKATKEPFTAFLLICGAWIFTAGYTSINAVVKAELFPTNIRALGVGLPYAITVSLFGGTAP
AIALYFKSIGHETWFYYYLSGVIFLSLLIYATMRDTKHASAMHRHE

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=409, Percent_Identity=48.8997555012225, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1790550, Length=447, Percent_Identity=30.8724832214765, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=30.2961275626424, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789941, Length=443, Percent_Identity=28.8939051918736, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=31.4285714285714, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082404, Length=351, Percent_Identity=24.7863247863248, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48847; Mature: 48716

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRPDMTDQAIEPAVEPATSIADVERRIKAILIGSAGNLVEWYDFYAYTAFALYFAPAF
CCCCCCCCHHHHCCHHCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
FPSKDPVVEQLNAAVVFAATFLMRPLGGWLFGYLADQYGRRLSLTISVLCMCFGSLIIAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
TPTYATIGIAAPIILALARVIEGLSLGGEYGASATYLSEVADAKHRGFYSSFQYVTLIGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHCCC
QLTAIIVLLLLQKVFLTTDELKAWGWRIPFFIGACLAIFVAVMRRNLHETEAFVEAKKKA
HHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GSSSSMAALFRYPRELLLVVGLTAGGTAAFYTFTTYMQTFVKLSVGLTEDQTTMVIFGSL
CCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LFACVLQPLYGAISDRIGRKPLLIFFGIAGTLSTIPILTALKATKEPFTAFLLICGAWIF
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAGYTSINAVVKAELFPTNIRALGVGLPYAITVSLFGGTAPAIALYFKSIGHETWFYYYL
HCCCHHHHHHHEEEECCCCHHHEECCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHH
SGVIFLSLLIYATMRDTKHASAMHRHE
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRRPDMTDQAIEPAVEPATSIADVERRIKAILIGSAGNLVEWYDFYAYTAFALYFAPAF
CCCCCCCHHHHCCHHCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHC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HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]