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The map label for this gene is 146339541

Identifier: 146339541

GI number: 146339541

Start: 2705009

End: 2706433

Strand: Reverse

Name: 146339541

Synonym: BRADO2532

Alternate gene names: NA

Gene position: 2706433-2705009 (Counterclockwise)

Preceding gene: 146339542

Following gene: 146339540

Centisome position: 36.3

GC content: 68.0

Gene sequence:

>1425_bases
TTGGCCGACCAGGAATTGATCCGCGAGACCGCCTGCGCCGTCGTCGACAGGCTGAAGGCCGGCGACATCACGCCGCTCGA
TTGCCTCGACGCGCTGGAAGCGCGCATCAAGGTCGTCGACGACAAGGTCAACGCGCTGCCAACGCTGTGCTTCGACCGCG
CCCGCGATCACGCCAAGGCGCTGATGAGCAAGCCGGCCCATGAGCGCGGCCTGCTCGCCGGCCTGCCCATCCCGATCAAG
GATCTCACCGACGTCCAGGGCGTGCTGACCACCCAGGGCTCGCCGATCTTCAAGGACCGCATTCCGGAACGCTCCGATCT
CCTGGTCGAGCATCTCGAAGCCAATGGCGGCGTGATCTACGCCAAGTCGAACACGCCGGAGTTCGGCGCCGGCGCCAACA
CCTTCAACGAGGTGTTTGGCGCGACGCGCAATCCCTGGGACACGTCCCGCTCGGCCGCCGGTTCCTCCGGCGGCGCCGCG
GTGGCACTCGCGACCGGCATGGCCTGGCTGGCGCATGGCTCGGACATGGGCGGCTCGCTGCGAAATCCTGCGAGCTTCTG
CGGCATCATCGGCCTGCGCCCGAGCTTCGGCCGCGTCGCGCATACGCCGGCCACATCCGTCGACCGCAACCTCGGCGTCC
AGGGCCCGATGGCCCGGAATGTCGAGGACCTCGCGCTGCTCTTGGATGCGATGAGCGGCGAGCATGCCGCCGATCCGATC
TCGCTGCCCTCGCCCGCGACCTCGTTCCTGTCCGCCGCCCGCTCCGGCAAGAAGCCAGTGCGCATCGCCTACTCGCCCGA
TCTCGGCATCACGCCGGTCGACCCCGAAGTGATCGCCGTGACCCGCAAGGCCGCGCAGCGCTTCTCTGAGTTGGGCGTGA
TCGTCGAGGAAGCGCATCCTGATTTCAGCGAGGCGCATGAATGCTTCCACGTGCTGCGCGCGCTCGACTTCGCGATGAGC
AAGCAGGAGCTGCTGCGCACCAAGCGCGACCTGCTGAAGCCCGAAGTGATCTGGAACATCGAGGAAGGCCTGAAGCTTTC
GGTCGCGCAGATCGCGCGCGCCGAGGCACAGCGCGTGGCGATCACCGCGCGCGCGGTGAAGTTCTTCGAGACCTATGATC
TGCTGCTGGCGCCGGCGACGATCGTGCCGCCCTTCCCGGTCGAGAACCGCTATGTCGCCGAATGCGCCGGGCACACATTC
GACAATTACGTGCAATGGCTCGGCATCGTCTACGCGATCACGCTCGCCTGCTGCCCGGCGCTGTCGCTGCCCTGCGGCTT
CACGGCCTCCGGCCTGCCTGTCGGCCTGCAGATGATCGCGCGCCCGCGCAACGACGCCGGCCTGCTCGCGGGTGCCAAGG
TGCTGGAAGACATCCTCGGCGTCGGCAGCTCCACCCCGATCGACCCACGCGCGCCGAAGGGCTGA

Upstream 100 bases:

>100_bases
ATCAGCGCGGGGTGGGGTTGAGCGCAGCGATCTTTCCACACCGGACGAAACGTGGTCTACAGCACGGCGCCGGAACGCTT
CCAATCAGGAGAACCACGCC

Downstream 100 bases:

>100_bases
GCGGACCAAAATCGTAGCCCGGATGAGCGGGAGCCATCGCCCGAAGGGCGAAGGCGGACGCGACATCCGGGTTCTCACGC
GCTATCGCCGGTGAACCCGG

Product: putative amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MADQELIRETACAVVDRLKAGDITPLDCLDALEARIKVVDDKVNALPTLCFDRARDHAKALMSKPAHERGLLAGLPIPIK
DLTDVQGVLTTQGSPIFKDRIPERSDLLVEHLEANGGVIYAKSNTPEFGAGANTFNEVFGATRNPWDTSRSAAGSSGGAA
VALATGMAWLAHGSDMGGSLRNPASFCGIIGLRPSFGRVAHTPATSVDRNLGVQGPMARNVEDLALLLDAMSGEHAADPI
SLPSPATSFLSAARSGKKPVRIAYSPDLGITPVDPEVIAVTRKAAQRFSELGVIVEEAHPDFSEAHECFHVLRALDFAMS
KQELLRTKRDLLKPEVIWNIEEGLKLSVAQIARAEAQRVAITARAVKFFETYDLLLAPATIVPPFPVENRYVAECAGHTF
DNYVQWLGIVYAITLACCPALSLPCGFTASGLPVGLQMIARPRNDAGLLAGAKVLEDILGVGSSTPIDPRAPKG

Sequences:

>Translated_474_residues
MADQELIRETACAVVDRLKAGDITPLDCLDALEARIKVVDDKVNALPTLCFDRARDHAKALMSKPAHERGLLAGLPIPIK
DLTDVQGVLTTQGSPIFKDRIPERSDLLVEHLEANGGVIYAKSNTPEFGAGANTFNEVFGATRNPWDTSRSAAGSSGGAA
VALATGMAWLAHGSDMGGSLRNPASFCGIIGLRPSFGRVAHTPATSVDRNLGVQGPMARNVEDLALLLDAMSGEHAADPI
SLPSPATSFLSAARSGKKPVRIAYSPDLGITPVDPEVIAVTRKAAQRFSELGVIVEEAHPDFSEAHECFHVLRALDFAMS
KQELLRTKRDLLKPEVIWNIEEGLKLSVAQIARAEAQRVAITARAVKFFETYDLLLAPATIVPPFPVENRYVAECAGHTF
DNYVQWLGIVYAITLACCPALSLPCGFTASGLPVGLQMIARPRNDAGLLAGAKVLEDILGVGSSTPIDPRAPKG
>Mature_473_residues
ADQELIRETACAVVDRLKAGDITPLDCLDALEARIKVVDDKVNALPTLCFDRARDHAKALMSKPAHERGLLAGLPIPIKD
LTDVQGVLTTQGSPIFKDRIPERSDLLVEHLEANGGVIYAKSNTPEFGAGANTFNEVFGATRNPWDTSRSAAGSSGGAAV
ALATGMAWLAHGSDMGGSLRNPASFCGIIGLRPSFGRVAHTPATSVDRNLGVQGPMARNVEDLALLLDAMSGEHAADPIS
LPSPATSFLSAARSGKKPVRIAYSPDLGITPVDPEVIAVTRKAAQRFSELGVIVEEAHPDFSEAHECFHVLRALDFAMSK
QELLRTKRDLLKPEVIWNIEEGLKLSVAQIARAEAQRVAITARAVKFFETYDLLLAPATIVPPFPVENRYVAECAGHTFD
NYVQWLGIVYAITLACCPALSLPCGFTASGLPVGLQMIARPRNDAGLLAGAKVLEDILGVGSSTPIDPRAPKG

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=27.1457085828343, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=24.8459958932238, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI166795287, Length=206, Percent_Identity=35.9223300970874, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=27.8884462151394, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71990152, Length=310, Percent_Identity=28.3870967741935, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=284, Percent_Identity=28.8732394366197, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=268, Percent_Identity=28.7313432835821, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=26.3274336283186, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538254, Length=411, Percent_Identity=24.330900243309, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=322, Percent_Identity=27.9503105590062, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=322, Percent_Identity=27.9503105590062, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=239, Percent_Identity=30.1255230125523, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=418, Percent_Identity=27.9904306220096, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=195, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=26.0692464358452, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648113, Length=479, Percent_Identity=26.9311064718163, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648435, Length=496, Percent_Identity=25.4032258064516, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648437, Length=496, Percent_Identity=25.4032258064516, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550774, Length=496, Percent_Identity=25.4032258064516, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648441, Length=420, Percent_Identity=27.1428571428571, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648439, Length=420, Percent_Identity=27.1428571428571, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=26.6802443991853, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=27.3279352226721, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=27.3279352226721, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=27.3279352226721, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=27.3279352226721, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=246, Percent_Identity=31.7073170731707, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 50476; Mature: 50345

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQELIRETACAVVDRLKAGDITPLDCLDALEARIKVVDDKVNALPTLCFDRARDHAKA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMSKPAHERGLLAGLPIPIKDLTDVQGVLTTQGSPIFKDRIPERSDLLVEHLEANGGVIY
HHHCCCHHCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCEEE
AKSNTPEFGAGANTFNEVFGATRNPWDTSRSAAGSSGGAAVALATGMAWLAHGSDMGGSL
ECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RNPASFCGIIGLRPSFGRVAHTPATSVDRNLGVQGPMARNVEDLALLLDAMSGEHAADPI
CCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
SLPSPATSFLSAARSGKKPVRIAYSPDLGITPVDPEVIAVTRKAAQRFSELGVIVEEAHP
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCC
DFSEAHECFHVLRALDFAMSKQELLRTKRDLLKPEVIWNIEEGLKLSVAQIARAEAQRVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCHHHHHHHHHHHHHHH
ITARAVKFFETYDLLLAPATIVPPFPVENRYVAECAGHTFDNYVQWLGIVYAITLACCPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LSLPCGFTASGLPVGLQMIARPRNDAGLLAGAKVLEDILGVGSSTPIDPRAPKG
CCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADQELIRETACAVVDRLKAGDITPLDCLDALEARIKVVDDKVNALPTLCFDRARDHAKA
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMSKPAHERGLLAGLPIPIKDLTDVQGVLTTQGSPIFKDRIPERSDLLVEHLEANGGVIY
HHHCCCHHCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCEEE
AKSNTPEFGAGANTFNEVFGATRNPWDTSRSAAGSSGGAAVALATGMAWLAHGSDMGGSL
ECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RNPASFCGIIGLRPSFGRVAHTPATSVDRNLGVQGPMARNVEDLALLLDAMSGEHAADPI
CCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
SLPSPATSFLSAARSGKKPVRIAYSPDLGITPVDPEVIAVTRKAAQRFSELGVIVEEAHP
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCC
DFSEAHECFHVLRALDFAMSKQELLRTKRDLLKPEVIWNIEEGLKLSVAQIARAEAQRVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCHHHHHHHHHHHHHHH
ITARAVKFFETYDLLLAPATIVPPFPVENRYVAECAGHTFDNYVQWLGIVYAITLACCPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LSLPCGFTASGLPVGLQMIARPRNDAGLLAGAKVLEDILGVGSSTPIDPRAPKG
CCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]