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The map label for this gene is echA12 [H]

Identifier: 146339340

GI number: 146339340

Start: 2472883

End: 2473719

Strand: Direct

Name: echA12 [H]

Synonym: BRADO2321

Alternate gene names: 146339340

Gene position: 2472883-2473719 (Clockwise)

Preceding gene: 146339339

Following gene: 146339341

Centisome position: 33.16

GC content: 63.92

Gene sequence:

>837_bases
ATGTCCAAGCCCGCCAATCCCGTCACCGTGCCGTTGGCTGACTACAAGCCCAGCCATTTCCTGCTGACGGTCAAGGATCG
CGTCGCGACGGTGACGCTGAACCGGCCGGAGCGGAAGAATCCGCTCACGTTCGAGAGCTATCGCGAGTTGACGGACTTCT
TCCGCGCCTGCGCGTTCGACAACGACGTCAAGGCAATTGTCGTGACCGGCGCGGGCGGCAATTTCTCCTCTGGCGGCGAC
GTGTTCGAGATCATCGGTCCGCTGGTCAAGATGGACACCAAGGGTCTGACCGCGTTCACGCGGATGACTGGCGACCTCGT
CAAAGCGATGCGCGCCTGTCCGCAGCCGATCGTTGCTGCTGTCGAGGGGATCTGCGCGGGGGCGGGCGCGATCGTTGCGA
TGGCCTCGGACATCAGGCTCGCCGCGACCGGCGCCAAGGTCGCGTTCCTGTTCAACAAGGTCGGCCTGGCTGGCTGCGAC
ATGGGCGCCTGTGCGATCCTGCCGCGGATCATCGGCCAGTCCCGCGCCTCCGAGCTGCTCTACACCGGCCGCTTCATGAG
CGCCGACGAGGGCGAGCGGTGGGGCTTCTTCAGTCGGATCGTCGCTCCCGCTGACGTTCTGGCTCAGGCGGTGACGTTGG
CCGCCGAGATCGCGAATGGTCCGACCTTCGCCAACACAATGACTAAGCGCATGCTGGCGATGGAATGGGCGATGTCCGTC
GAGGAAGCGATTGAGGCGGAGGCAGTTGCCCAGGCCTTGTGCATGACGACAGAGGATTTCAACCGCGCGTTCGAAGCTTT
TGCGGCAAAGTCGAAGCCGGTCTTCCAGGGGAATTGA

Upstream 100 bases:

>100_bases
TGAGGGGGCTGGCGCCATCACGGGGCAAGCGATCGCAGTGGCGGGCGGCGAAGTCTAGGCCGCGTCGTCCAATCAGAACA
ATCGAACCGAGGGAGCTCAC

Downstream 100 bases:

>100_bases
GCAGCTGTAGGAACCGAAGGGGCTTGCCAGGAAATATTTTAGGTCTAAAATAATTTCCATTGGCTCGGCTCTCCAGTGGA
GGCGCTCATGAAGATCGCGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSKPANPVTVPLADYKPSHFLLTVKDRVATVTLNRPERKNPLTFESYRELTDFFRACAFDNDVKAIVVTGAGGNFSSGGD
VFEIIGPLVKMDTKGLTAFTRMTGDLVKAMRACPQPIVAAVEGICAGAGAIVAMASDIRLAATGAKVAFLFNKVGLAGCD
MGACAILPRIIGQSRASELLYTGRFMSADEGERWGFFSRIVAPADVLAQAVTLAAEIANGPTFANTMTKRMLAMEWAMSV
EEAIEAEAVAQALCMTTEDFNRAFEAFAAKSKPVFQGN

Sequences:

>Translated_278_residues
MSKPANPVTVPLADYKPSHFLLTVKDRVATVTLNRPERKNPLTFESYRELTDFFRACAFDNDVKAIVVTGAGGNFSSGGD
VFEIIGPLVKMDTKGLTAFTRMTGDLVKAMRACPQPIVAAVEGICAGAGAIVAMASDIRLAATGAKVAFLFNKVGLAGCD
MGACAILPRIIGQSRASELLYTGRFMSADEGERWGFFSRIVAPADVLAQAVTLAAEIANGPTFANTMTKRMLAMEWAMSV
EEAIEAEAVAQALCMTTEDFNRAFEAFAAKSKPVFQGN
>Mature_277_residues
SKPANPVTVPLADYKPSHFLLTVKDRVATVTLNRPERKNPLTFESYRELTDFFRACAFDNDVKAIVVTGAGGNFSSGGDV
FEIIGPLVKMDTKGLTAFTRMTGDLVKAMRACPQPIVAAVEGICAGAGAIVAMASDIRLAATGAKVAFLFNKVGLAGCDM
GACAILPRIIGQSRASELLYTGRFMSADEGERWGFFSRIVAPADVLAQAVTLAAEIANGPTFANTMTKRMLAMEWAMSVE
EAIEAEAVAQALCMTTEDFNRAFEAFAAKSKPVFQGN

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=31.2252964426877, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI68989263, Length=198, Percent_Identity=28.2828282828283, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=25.6916996047431, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=27.843137254902, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI45643119, Length=275, Percent_Identity=24.3636363636364, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI260275230, Length=275, Percent_Identity=24.3636363636364, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI260274832, Length=275, Percent_Identity=24.3636363636364, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI37594471, Length=154, Percent_Identity=31.1688311688312, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI37594469, Length=154, Percent_Identity=31.1688311688312, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=31.8007662835249, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=28.7878787878788, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=27.8625954198473, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=229, Percent_Identity=29.6943231441048, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=231, Percent_Identity=26.4069264069264, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=28.4584980237154, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=26.0700389105058, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=28.4, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=181, Percent_Identity=29.2817679558011, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=30.5019305019305, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=28.5140562248996, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=28.5140562248996, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=29.7297297297297, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357171, Length=272, Percent_Identity=25.7352941176471, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24650670, Length=222, Percent_Identity=28.8288288288288, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=25.390625, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29640; Mature: 29508

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPANPVTVPLADYKPSHFLLTVKDRVATVTLNRPERKNPLTFESYRELTDFFRACAFD
CCCCCCCEEEEECCCCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
NDVKAIVVTGAGGNFSSGGDVFEIIGPLVKMDTKGLTAFTRMTGDLVKAMRACPQPIVAA
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VEGICAGAGAIVAMASDIRLAATGAKVAFLFNKVGLAGCDMGACAILPRIIGQSRASELL
HHHHHCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHH
YTGRFMSADEGERWGFFSRIVAPADVLAQAVTLAAEIANGPTFANTMTKRMLAMEWAMSV
HCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EEAIEAEAVAQALCMTTEDFNRAFEAFAAKSKPVFQGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SKPANPVTVPLADYKPSHFLLTVKDRVATVTLNRPERKNPLTFESYRELTDFFRACAFD
CCCCCCEEEEECCCCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
NDVKAIVVTGAGGNFSSGGDVFEIIGPLVKMDTKGLTAFTRMTGDLVKAMRACPQPIVAA
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VEGICAGAGAIVAMASDIRLAATGAKVAFLFNKVGLAGCDMGACAILPRIIGQSRASELL
HHHHHCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHH
YTGRFMSADEGERWGFFSRIVAPADVLAQAVTLAAEIANGPTFANTMTKRMLAMEWAMSV
HCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EEAIEAEAVAQALCMTTEDFNRAFEAFAAKSKPVFQGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]