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The map label for this gene is hyuA [C]

Identifier: 146338853

GI number: 146338853

Start: 1912364

End: 1913833

Strand: Direct

Name: hyuA [C]

Synonym: BRADO1792

Alternate gene names: 146338853

Gene position: 1912364-1913833 (Clockwise)

Preceding gene: 146338851

Following gene: 146338854

Centisome position: 25.65

GC content: 66.67

Gene sequence:

>1470_bases
ATGACCGAGCCGGCCTATGACCTGATCATCCGGGGAGGGCGCGTCGCCACCACCACCGACACGTTCGAGGCCGATGTCGC
CGTCTCCGGCGAGACCATCGCGGCGATCGGTCGCGGGCTCGGACCTGCGAAGCGCGAGATCGACGCGCGCGGCAAATACG
TACTGCCCGGCGGCGTCGACAGCCATTGCCACATCGAGCAGCTCTCGGCCGCGGGTATCGTCAATGCAGATACGTTCGAG
AGCGCGACGACCTCTGCGGCGTTTGGCGGCACGACGTCAGTGATTTCGTTCGCCGCGCAGCATGTGGGTATGCAGCTGCC
GCAGGTGCTCGAGGAGTATCACGCGCTGGCGCGCAAGGGCGCGGTCATCGACTATGCGTTCCACATGATCATCGCGGATC
CGACCAAGGACACCCTGGAGCGCGATCTGCCGGCGCTGATCCGGCAGGGCCACGGCTCGATCAAGATCTTCATGACCTAT
GACCGGCTCAAGATCGAAGACGAGCCGCTGCTCGACATTCTCGTCGCCGCGCGCGAGGGCGGCGCGATGCTGTGCGCGCA
TGCCGAGAACCACGGCATCATTTCCTGGATGGTGAAGCGGCTGCTGGCGCGCGGCTACACCGCGCCGAAATATCACGCCA
TCAGCCATGCGCGGTTCTCCGAGGCGGAGGCGTTCAACCGGCTGATCGGTATGGCCGCGCTGGTCGATCAGCCGATCATG
ATCTTCCACGTTTCGACCGCCGAAGGCGCCAGGGTCATCCGCGACGCGCGCGGGCAGGGGCTGAAGGTGTTCGCGGAGAC
CTGCCCGCAATATCTGTTCATGACGGCCGACGATTTGGACAAGCCAGGCATCGAGGGCGCGAAGTGGATGTGCAGCCCGC
CGCCGCGCCGCGCGCCGGACCAGGAGGCGCTGTGGCAGGCGCTGTCGCTCGGCGACCTGCAGACGATCTCGTCGGACCAC
GCGCCGTATCGCTTCGACGAGACCGGCAAGCTGCGCGCCGGTCCCAATCCGAACTTCAAGCAGATCGCCAACGGCCTGCC
GGGCCTCGAGCTGCGGCTGCCGCTGCTGTTCGACGCGATGGTGTCGAAGGGCCGGCTCGGCCTGAACAAGTTCGTCGAGC
TGACGGCGACAGCGCCGGCCAAGATCTACAATCTGCATCCGCGCAAGGGCGCGATCGCGATCGGGGCCGATGCAGATATC
GCGCTGTGGGATCCGGACAAGCGGGTGACGATTGCTGATGACAGCATGCACGATCTCGCCGGCTACACGCCGTTCGCGGG
GCGCAGCGTGCAGGGCTGGCCTGTGACCGTTCTGTCGCGCGGTCGGGTGATTGTCGATGATGGCAAGATCGCGGTGCAGC
CCGGCTCCGGCCAGTTCCTCGCCCGCAGCGGCGGCGAGGCGGCGAAGCCGACGGGACGTCTGGTGGCGGACATGGACCCC
GAGCAGAACTTTGGGGCGACGCTGCTGTGA

Upstream 100 bases:

>100_bases
CGTCGCGCGACAGGCAATCACTATGCCACGGCGGACGCAAGCGGGCCGGCGCGGCCTTCCAGCATCACGCCGTTCCATCT
GGCAGAACTAGGAGATCGCG

Downstream 100 bases:

>100_bases
GCCTGTTGGCGAAGCATCGCGGGCCTCGTCCTTCGAGACGGTCGCTTCGCGACCTCCTCAGGATGAGGGGACAGAGCAGG
TCAGGCGGCGCGCACGCTGC

Product: dihydropyrimidinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTEPAYDLIIRGGRVATTTDTFEADVAVSGETIAAIGRGLGPAKREIDARGKYVLPGGVDSHCHIEQLSAAGIVNADTFE
SATTSAAFGGTTSVISFAAQHVGMQLPQVLEEYHALARKGAVIDYAFHMIIADPTKDTLERDLPALIRQGHGSIKIFMTY
DRLKIEDEPLLDILVAAREGGAMLCAHAENHGIISWMVKRLLARGYTAPKYHAISHARFSEAEAFNRLIGMAALVDQPIM
IFHVSTAEGARVIRDARGQGLKVFAETCPQYLFMTADDLDKPGIEGAKWMCSPPPRRAPDQEALWQALSLGDLQTISSDH
APYRFDETGKLRAGPNPNFKQIANGLPGLELRLPLLFDAMVSKGRLGLNKFVELTATAPAKIYNLHPRKGAIAIGADADI
ALWDPDKRVTIADDSMHDLAGYTPFAGRSVQGWPVTVLSRGRVIVDDGKIAVQPGSGQFLARSGGEAAKPTGRLVADMDP
EQNFGATLL

Sequences:

>Translated_489_residues
MTEPAYDLIIRGGRVATTTDTFEADVAVSGETIAAIGRGLGPAKREIDARGKYVLPGGVDSHCHIEQLSAAGIVNADTFE
SATTSAAFGGTTSVISFAAQHVGMQLPQVLEEYHALARKGAVIDYAFHMIIADPTKDTLERDLPALIRQGHGSIKIFMTY
DRLKIEDEPLLDILVAAREGGAMLCAHAENHGIISWMVKRLLARGYTAPKYHAISHARFSEAEAFNRLIGMAALVDQPIM
IFHVSTAEGARVIRDARGQGLKVFAETCPQYLFMTADDLDKPGIEGAKWMCSPPPRRAPDQEALWQALSLGDLQTISSDH
APYRFDETGKLRAGPNPNFKQIANGLPGLELRLPLLFDAMVSKGRLGLNKFVELTATAPAKIYNLHPRKGAIAIGADADI
ALWDPDKRVTIADDSMHDLAGYTPFAGRSVQGWPVTVLSRGRVIVDDGKIAVQPGSGQFLARSGGEAAKPTGRLVADMDP
EQNFGATLL
>Mature_488_residues
TEPAYDLIIRGGRVATTTDTFEADVAVSGETIAAIGRGLGPAKREIDARGKYVLPGGVDSHCHIEQLSAAGIVNADTFES
ATTSAAFGGTTSVISFAAQHVGMQLPQVLEEYHALARKGAVIDYAFHMIIADPTKDTLERDLPALIRQGHGSIKIFMTYD
RLKIEDEPLLDILVAAREGGAMLCAHAENHGIISWMVKRLLARGYTAPKYHAISHARFSEAEAFNRLIGMAALVDQPIMI
FHVSTAEGARVIRDARGQGLKVFAETCPQYLFMTADDLDKPGIEGAKWMCSPPPRRAPDQEALWQALSLGDLQTISSDHA
PYRFDETGKLRAGPNPNFKQIANGLPGLELRLPLLFDAMVSKGRLGLNKFVELTATAPAKIYNLHPRKGAIAIGADADIA
LWDPDKRVTIADDSMHDLAGYTPFAGRSVQGWPVTVLSRGRVIVDDGKIAVQPGSGQFLARSGGEAAKPTGRLVADMDPE
QNFGATLL

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin. Has no activity on dihydropyrimidines [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503051, Length=461, Percent_Identity=34.2733188720174, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI62422571, Length=461, Percent_Identity=34.2733188720174, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI190194363, Length=462, Percent_Identity=35.0649350649351, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI4503375, Length=465, Percent_Identity=34.1935483870968, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI4503377, Length=462, Percent_Identity=33.1168831168831, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI4503379, Length=462, Percent_Identity=32.034632034632, Blast_Score=232, Evalue=8e-61,
Organism=Homo sapiens, GI19923821, Length=459, Percent_Identity=32.244008714597, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI18105007, Length=443, Percent_Identity=27.5395033860045, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI87082175, Length=461, Percent_Identity=35.3579175704989, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1786722, Length=468, Percent_Identity=29.4871794871795, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71989490, Length=465, Percent_Identity=32.0430107526882, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17539558, Length=459, Percent_Identity=30.2832244008715, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI86575075, Length=457, Percent_Identity=24.0700218818381, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI193204318, Length=407, Percent_Identity=27.5184275184275, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322218, Length=432, Percent_Identity=25.462962962963, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI17137462, Length=460, Percent_Identity=31.9565217391304, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI221377917, Length=465, Percent_Identity=33.1182795698925, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24642586, Length=429, Percent_Identity=29.3706293706294, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24644287, Length=303, Percent_Identity=31.023102310231, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24644289, Length=234, Percent_Identity=31.6239316239316, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859883, Length=239, Percent_Identity=30.1255230125523, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 52670; Mature: 52539

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPAYDLIIRGGRVATTTDTFEADVAVSGETIAAIGRGLGPAKREIDARGKYVLPGGVD
CCCCCEEEEEECCEEEEECCCCEEEEEECCCHHHHHHCCCCHHHHHHCCCCCEEECCCCC
SHCHIEQLSAAGIVNADTFESATTSAAFGGTTSVISFAAQHVGMQLPQVLEEYHALARKG
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
AVIDYAFHMIIADPTKDTLERDLPALIRQGHGSIKIFMTYDRLKIEDEPLLDILVAAREG
CEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEHHEECCCC
GAMLCAHAENHGIISWMVKRLLARGYTAPKYHAISHARFSEAEAFNRLIGMAALVDQPIM
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IFHVSTAEGARVIRDARGQGLKVFAETCPQYLFMTADDLDKPGIEGAKWMCSPPPRRAPD
EEEEECCCCHHHHHHCCCCCEEHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
QEALWQALSLGDLQTISSDHAPYRFDETGKLRAGPNPNFKQIANGLPGLELRLPLLFDAM
HHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCCCEEEHHHHHHHH
VSKGRLGLNKFVELTATAPAKIYNLHPRKGAIAIGADADIALWDPDKRVTIADDSMHDLA
HHCCCCCCCHHEEEEECCCEEEEECCCCCCCEEECCCCEEEEECCCCEEEEECCCHHHHC
GYTPFAGRSVQGWPVTVLSRGRVIVDDGKIAVQPGSGQFLARSGGEAAKPTGRLVADMDP
CCCCCCCCCCCCCCEEEEECCEEEEECCEEEEECCCCCEEEECCCCCCCCCCCEEECCCC
EQNFGATLL
CCCCCCCCC
>Mature Secondary Structure 
TEPAYDLIIRGGRVATTTDTFEADVAVSGETIAAIGRGLGPAKREIDARGKYVLPGGVD
CCCCEEEEEECCEEEEECCCCEEEEEECCCHHHHHHCCCCHHHHHHCCCCCEEECCCCC
SHCHIEQLSAAGIVNADTFESATTSAAFGGTTSVISFAAQHVGMQLPQVLEEYHALARKG
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
AVIDYAFHMIIADPTKDTLERDLPALIRQGHGSIKIFMTYDRLKIEDEPLLDILVAAREG
CEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEHHEECCCC
GAMLCAHAENHGIISWMVKRLLARGYTAPKYHAISHARFSEAEAFNRLIGMAALVDQPIM
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IFHVSTAEGARVIRDARGQGLKVFAETCPQYLFMTADDLDKPGIEGAKWMCSPPPRRAPD
EEEEECCCCHHHHHHCCCCCEEHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
QEALWQALSLGDLQTISSDHAPYRFDETGKLRAGPNPNFKQIANGLPGLELRLPLLFDAM
HHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCCCEEEHHHHHHHH
VSKGRLGLNKFVELTATAPAKIYNLHPRKGAIAIGADADIALWDPDKRVTIADDSMHDLA
HHCCCCCCCHHEEEEECCCEEEEECCCCCCCEEECCCCEEEEECCCCEEEEECCCHHHHC
GYTPFAGRSVQGWPVTVLSRGRVIVDDGKIAVQPGSGQFLARSGGEAAKPTGRLVADMDP
CCCCCCCCCCCCCCEEEEECCEEEEECCEEEEECCCCCEEEECCCCCCCCCCCEEECCCC
EQNFGATLL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765480 [H]