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The map label for this gene is galE [C]

Identifier: 146338854

GI number: 146338854

Start: 1914006

End: 1914977

Strand: Direct

Name: galE [C]

Synonym: BRADO1793

Alternate gene names: 146338854

Gene position: 1914006-1914977 (Clockwise)

Preceding gene: 146338853

Following gene: 146338855

Centisome position: 25.67

GC content: 68.42

Gene sequence:

>972_bases
ATGCATGTCCTGATCTTCGGCGGCGCCGGCTTCGTCGGACTTAACACTGCGCAAGGACTGCTCGAGCGTGGGCATGCGGT
GACCGTATTCGATGCGGCACCGCTGCCGAGCGCGGCGCAGCGCGCCTTCGCACCGTTTGGTGAACGGCTGCACGTGATCA
ACGGCGACGTCATAGACGCCGACGCCGTCGCGGCCGCTATCGGCAATGGCTGCGATGCCGTCGTCATGGGCGCCGCGATC
ACCGCCGGTCCCGAACGCGATGCGTCCGATCCCGAGCGCATCCTCGCCGTGAATCTGCTCGCACAGGTACCGATCTTGCA
GGCCGCGCGGCGCAGCGGCGTCCGGCGCGTGATCAATCTGTCGTCGGCTGCATCCTACGGCGCCAGCGGCCAGCGCTTCG
CGGTGCTGGAGGAGACCACGCCGTGCGAACCGGTGTCGCTGTATGCGATCACGAAATTCACCTCCGAACGCGTCGCCGCG
CGACTGTCAGATCTTTGGCAGACCGACGTCATCAGCGTGCGCTTGTCCGCCGTGTTCGGGCCGTTCGAGCGCGGCGGGGG
CGTGCGCGATACGCCATCGCCGCAGGCGCTGATCGCGGCCTGCTGCGCGCGCAGCGAGCCGGCGTTGTTGAACGAAGCCG
GCGACAAGGACTGGGTCTACGCGGTCGATGTCGCCGATGCCGTGTGCCTGCTGCTCGAAGCGGAGAAGCCGCAGCAGACG
CTCTACAACATCTCGAACGAGGCGACGTTTACGGCCGAGGCGTGGGGCCGCGCCTTCGCGAGCGGTCGGTCCGGCTTCAC
CTGCCGCCTCGCGGAGCCGGGCGAAACGCCGACGGTGCCTGTGTTCGCGCCGGTCCGCGGCCGGCTGTCGACGATGCGGC
TGGCGAACGAGTTCGGCTGGCGCGCGCGGTTCGACTGCGCAGGCTCCGCCGAACATTTCGGCCGCTGGTTCGATCAGTAC
GGGAGGGATTGA

Upstream 100 bases:

>100_bases
AGAGCAGGTCAGGCGGCGCGCACGCTGCGTTTCCTCGTGGCGAGGAGGCTGCGCCAGCAGCCGTCTCGAACCATGAGGCC
CCGATGGAGAGGCTTTTCGC

Downstream 100 bases:

>100_bases
GATGCGGCTGAAGGATAAGGTCGCCATCATCACCGGCGGCGGCTCCGGCATCGGCCGCGCCAGCGCCGTGATGTTCGCGC
GCGAAGGTGCATTCATCGCG

Product: putative UDP-glucose 4-epimerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MHVLIFGGAGFVGLNTAQGLLERGHAVTVFDAAPLPSAAQRAFAPFGERLHVINGDVIDADAVAAAIGNGCDAVVMGAAI
TAGPERDASDPERILAVNLLAQVPILQAARRSGVRRVINLSSAASYGASGQRFAVLEETTPCEPVSLYAITKFTSERVAA
RLSDLWQTDVISVRLSAVFGPFERGGGVRDTPSPQALIAACCARSEPALLNEAGDKDWVYAVDVADAVCLLLEAEKPQQT
LYNISNEATFTAEAWGRAFASGRSGFTCRLAEPGETPTVPVFAPVRGRLSTMRLANEFGWRARFDCAGSAEHFGRWFDQY
GRD

Sequences:

>Translated_323_residues
MHVLIFGGAGFVGLNTAQGLLERGHAVTVFDAAPLPSAAQRAFAPFGERLHVINGDVIDADAVAAAIGNGCDAVVMGAAI
TAGPERDASDPERILAVNLLAQVPILQAARRSGVRRVINLSSAASYGASGQRFAVLEETTPCEPVSLYAITKFTSERVAA
RLSDLWQTDVISVRLSAVFGPFERGGGVRDTPSPQALIAACCARSEPALLNEAGDKDWVYAVDVADAVCLLLEAEKPQQT
LYNISNEATFTAEAWGRAFASGRSGFTCRLAEPGETPTVPVFAPVRGRLSTMRLANEFGWRARFDCAGSAEHFGRWFDQY
GRD
>Mature_323_residues
MHVLIFGGAGFVGLNTAQGLLERGHAVTVFDAAPLPSAAQRAFAPFGERLHVINGDVIDADAVAAAIGNGCDAVVMGAAI
TAGPERDASDPERILAVNLLAQVPILQAARRSGVRRVINLSSAASYGASGQRFAVLEETTPCEPVSLYAITKFTSERVAA
RLSDLWQTDVISVRLSAVFGPFERGGGVRDTPSPQALIAACCARSEPALLNEAGDKDWVYAVDVADAVCLLLEAEKPQQT
LYNISNEATFTAEAWGRAFASGRSGFTCRLAEPGETPTVPVFAPVRGRLSTMRLANEFGWRARFDCAGSAEHFGRWFDQY
GRD

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 34345; Mature: 34345

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLIFGGAGFVGLNTAQGLLERGHAVTVFDAAPLPSAAQRAFAPFGERLHVINGDVIDA
CEEEEEECCCEEECCHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCEEEEECCCEECH
DAVAAAIGNGCDAVVMGAAITAGPERDASDPERILAVNLLAQVPILQAARRSGVRRVINL
HHHHHHHCCCCCCEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAASYGASGQRFAVLEETTPCEPVSLYAITKFTSERVAARLSDLWQTDVISVRLSAVFG
HHHHCCCCCCCEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEHHHHHC
PFERGGGVRDTPSPQALIAACCARSEPALLNEAGDKDWVYAVDVADAVCLLLEAEKPQQT
CHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEHHHHHHHHHCCCCCHHH
LYNISNEATFTAEAWGRAFASGRSGFTCRLAEPGETPTVPVFAPVRGRLSTMRLANEFGW
HHCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RARFDCAGSAEHFGRWFDQYGRD
EEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHVLIFGGAGFVGLNTAQGLLERGHAVTVFDAAPLPSAAQRAFAPFGERLHVINGDVIDA
CEEEEEECCCEEECCHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCEEEEECCCEECH
DAVAAAIGNGCDAVVMGAAITAGPERDASDPERILAVNLLAQVPILQAARRSGVRRVINL
HHHHHHHCCCCCCEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAASYGASGQRFAVLEETTPCEPVSLYAITKFTSERVAARLSDLWQTDVISVRLSAVFG
HHHHCCCCCCCEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEHHHHHC
PFERGGGVRDTPSPQALIAACCARSEPALLNEAGDKDWVYAVDVADAVCLLLEAEKPQQT
CHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEHHHHHHHHHCCCCCHHH
LYNISNEATFTAEAWGRAFASGRSGFTCRLAEPGETPTVPVFAPVRGRLSTMRLANEFGW
HHCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RARFDCAGSAEHFGRWFDQYGRD
EEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]