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The map label for this gene is 146338851

Identifier: 146338851

GI number: 146338851

Start: 1910427

End: 1911353

Strand: Direct

Name: 146338851

Synonym: BRADO1790

Alternate gene names: NA

Gene position: 1910427-1911353 (Clockwise)

Preceding gene: 146338850

Following gene: 146338853

Centisome position: 25.62

GC content: 65.05

Gene sequence:

>927_bases
ATGCGCATCATCAACGTCGCCGCCGCCCAGATGGGGCCGATCCAGCGGGCCGACAGCCGGGAGGCCGTCGTCGCACGCAT
GATCGCGCTGCTGGACGAGGCCAAGCACAAGGGCGCCGACCTCATCGTCTATCCGGAGCTGGCGCTGACGACGTTCTTTC
CGCGCTGGTACATGGAGGATCAGGCCGAGGTCGATACATGGTTCGAGCGTGACATGCCCAACGCCGCCGTGCAGCCGCTG
TTCGATCGCGCCGCGCAGCATGAGATAGCGATGTATCTCGGCTATGCCGAGCTGACGCTGGACGGGCATCACTACAACAC
GGCTGTTCTGACCGATCGCAGCAGCAACATCGTCGGCAAGTATCGCAAGGTGCATCTGCCCGGCCACGAGGAATTCGAGC
CGGAGCGCAGCCACCAGCATCTGGAGAAGCGCTATTTCGAGCCGGGCGATCTCGGCTTTCCGGTCTGGCGCAATCTCGGA
GGGATCGTCGGGATGGCGATCTGCAACGACCGCCGCTGGCCGGAGACGTATCGCGTGATGGGTCTGCAGGATGTCGAGCT
GGTGCTGATCGGCTACAACACGCCGTCGGTGAACTCGCTGAAGTCGGCGGAAGGGCTGCAGCAGCGGCTGTTCCACAATC
GTCTCTCCGCGCAGGCCGGTGCGTATCAGAACTCGTGCTGGGTGGTGGCGGTCGCCAAGGCCGGCGTCGAGGACGGCCAT
CATCTGATCGGCGGCAGCCTGATCGTCAATCCCGACGGCGAGATCGTCGCGGAGGCGGTGACGGCAGGCGACGAGGTGCT
GGTCGCGGCGTGCGATATGGATGCGACGCGCTTCGGCAAGCAGACGATCTTCGATTTCGCCCGGCATCGACGGGTGGAGC
ACTATGGGCTGATCACCAGCCGCACGGGCGCGGTGCCGCCGGAGTGA

Upstream 100 bases:

>100_bases
CCGTGCGCGGCTGTGGTGGGCACGCTTCCGCCGTCCGTCGTCGATGCACTGAGCAAGACGCCGCTTTGCCCACCCTACGG
ACTGACTGTGGAGCTACACC

Downstream 100 bases:

>100_bases
GGCAGTTCGTGGTCAACCAATAGCGCAGTTCCAAATTCGGTGTCGTCCCGGCGAACGCCGGGACCCATACCGCGGAATCT
ATCGAGGGACGTCGCTGATA

Product: N-carbamoyl-D-amino acid hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRIINVAAAQMGPIQRADSREAVVARMIALLDEAKHKGADLIVYPELALTTFFPRWYMEDQAEVDTWFERDMPNAAVQPL
FDRAAQHEIAMYLGYAELTLDGHHYNTAVLTDRSSNIVGKYRKVHLPGHEEFEPERSHQHLEKRYFEPGDLGFPVWRNLG
GIVGMAICNDRRWPETYRVMGLQDVELVLIGYNTPSVNSLKSAEGLQQRLFHNRLSAQAGAYQNSCWVVAVAKAGVEDGH
HLIGGSLIVNPDGEIVAEAVTAGDEVLVAACDMDATRFGKQTIFDFARHRRVEHYGLITSRTGAVPPE

Sequences:

>Translated_308_residues
MRIINVAAAQMGPIQRADSREAVVARMIALLDEAKHKGADLIVYPELALTTFFPRWYMEDQAEVDTWFERDMPNAAVQPL
FDRAAQHEIAMYLGYAELTLDGHHYNTAVLTDRSSNIVGKYRKVHLPGHEEFEPERSHQHLEKRYFEPGDLGFPVWRNLG
GIVGMAICNDRRWPETYRVMGLQDVELVLIGYNTPSVNSLKSAEGLQQRLFHNRLSAQAGAYQNSCWVVAVAKAGVEDGH
HLIGGSLIVNPDGEIVAEAVTAGDEVLVAACDMDATRFGKQTIFDFARHRRVEHYGLITSRTGAVPPE
>Mature_308_residues
MRIINVAAAQMGPIQRADSREAVVARMIALLDEAKHKGADLIVYPELALTTFFPRWYMEDQAEVDTWFERDMPNAAVQPL
FDRAAQHEIAMYLGYAELTLDGHHYNTAVLTDRSSNIVGKYRKVHLPGHEEFEPERSHQHLEKRYFEPGDLGFPVWRNLG
GIVGMAICNDRRWPETYRVMGLQDVELVLIGYNTPSVNSLKSAEGLQQRLFHNRLSAQAGAYQNSCWVVAVAKAGVEDGH
HLIGGSLIVNPDGEIVAEAVTAGDEVLVAACDMDATRFGKQTIFDFARHRRVEHYGLITSRTGAVPPE

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17533173, Length=319, Percent_Identity=23.5109717868339, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 34453; Mature: 34453

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIINVAAAQMGPIQRADSREAVVARMIALLDEAKHKGADLIVYPELALTTFFPRWYMED
CEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCC
QAEVDTWFERDMPNAAVQPLFDRAAQHEIAMYLGYAELTLDGHHYNTAVLTDRSSNIVGK
CHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCEEEEECCCCCCCCC
YRKVHLPGHEEFEPERSHQHLEKRYFEPGDLGFPVWRNLGGIVGMAICNDRRWPETYRVM
EEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCHHEEEEEECCCCCCHHHHEE
GLQDVELVLIGYNTPSVNSLKSAEGLQQRLFHNRLSAQAGAYQNSCWVVAVAKAGVEDGH
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
HLIGGSLIVNPDGEIVAEAVTAGDEVLVAACDMDATRFGKQTIFDFARHRRVEHYGLITS
EEECCEEEECCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHCEEEC
RTGAVPPE
CCCCCCCC
>Mature Secondary Structure
MRIINVAAAQMGPIQRADSREAVVARMIALLDEAKHKGADLIVYPELALTTFFPRWYMED
CEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCC
QAEVDTWFERDMPNAAVQPLFDRAAQHEIAMYLGYAELTLDGHHYNTAVLTDRSSNIVGK
CHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCEEEEECCCCCCCCC
YRKVHLPGHEEFEPERSHQHLEKRYFEPGDLGFPVWRNLGGIVGMAICNDRRWPETYRVM
EEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCHHEEEEEECCCCCCHHHHEE
GLQDVELVLIGYNTPSVNSLKSAEGLQQRLFHNRLSAQAGAYQNSCWVVAVAKAGVEDGH
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
HLIGGSLIVNPDGEIVAEAVTAGDEVLVAACDMDATRFGKQTIFDFARHRRVEHYGLITS
EEECCEEEECCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHCEEEC
RTGAVPPE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]