| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is fadD [H]
Identifier: 146338040
GI number: 146338040
Start: 996504
End: 998372
Strand: Direct
Name: fadD [H]
Synonym: BRADO0933
Alternate gene names: 146338040
Gene position: 996504-998372 (Clockwise)
Preceding gene: 146338039
Following gene: 146338041
Centisome position: 13.36
GC content: 66.51
Gene sequence:
>1869_bases ATGGACGGGACGGCAGCGAAGGTGTTGGAGAAGCCGGCATTCCGCAAGGTCAACTGGCTGGCGCGCGACATCGACGTCGA GCGGCGCGCGGACGGCGTGATCGTCATCAAGTCGCGCATTCCCCTTCAAGCCTATGAGCCGCATCTCGCCGCGCCGCTGG CGCGCTGGGCCAAGGAAGCGCCGACGCGGACCTGGCTCGCGCAGCGCAGCGGCCCCGAGCGCCAGTGGCGCAAGCTGCCT TATGCCGAGGCGAAGCGCACCATCGATGGGCTGACGCAAGGGCTGCTCAATCTGAAGCTTGACGGCCGCCCGGTGGCGAT CCTCTCCGGCAATTCCATCGAGCACGCGCTGATGACCTTCGCCGCGATGCAGGCGCGATCGCCGGCGGCGCCGGTGTCGC CGGCCTATTCGCTCATGAGCCACGACCACCTCAAGCTCAAATATCTCTTCGGGCTGGTGAAGCCGGGCGTGGTCATGGTG CAGGACGGCCCGACCTTCGAGAAGGCGCTGAAAGCGCTCGATCTCGACGGCGTCACCGTCGTGCATGTCGCGCGTCCCTG CGAGGGCATTCCGAGCGTCAGCTTCGCCGATCTCGCCGCGACGCCCGTCACCGGCGACGTTGATGCCTCGATCGCGCAGA TCACGCCCGAGACTGTGGGCAAGCTGCTGTTCACCTCCGGTTCGACCGGCATGCCCAAGGCGGTCATCAACACGCAGCGC ATGATGTGCGCCAATGCGGCGATGATGATGCAGACGCGTCCGCGCGCGCCGGATGCGCCGGTCTCCACGGTGCTGGACTG GATGCCGTGGAATCACACCATGGGCGGCAATGCGGCGTTCAATCCGCTGCTGATCGATGGCGGCACGCTCTACATCGACG ACGGCCGGCCGATGCCCGGCATGTTCGACGAGACGATCAAGAATCTGCGCGAGGTGTCGCCGACCTACTACGCCAACGTT CCCGCAGGCTACGCCGCGCTCGCTGCGGCGATGGAGAAGGACGACGCGCTGTGCCGCTCGTTCTTCAAGGACCTCGGCAT CATGGCGTATGGCGGCGCGCGGCTGCCGGACGATCTCTATGAGCGCATGCAGGCGCTGGCGGTCAAGACCACCGGCGAGC GCATCGTGTTCTACACCGGCTGGGGCTCGACCGAGACCGCGCCGACCTCGACCGGCACCTATTGGAACACCGAGCGCGTC GGCCTGATCGGCCTGCCCTTCCCCGGTGTCGAGCTGAAGATGGTTCCGACCGGCGATAAATACGAGCTGCGGCTGCGCGG CATCAACGTGATGCCCGGCTATTTCGGCCAACCCGAGCTGACCAGGAAGGCGTTCGACGACGAAGGCTTCTACTGCATCG GCGACGCCGGCGTGTTCGTCGATCCCAATGATCCCGCGCAGGGCATCATCTTCGCCGGCCGTGTCGTCGAGGACTTCAAG TTGACCACCGGCACCTTCGTCCATGTCGGCTCGCTGCGCACCGATGCCATTGCCGCGGCCACGCCGGCGATCCATGACGC GCTCGTGGCGGGGCAGGACCGCGAATATATCGGCCTGCTGGCGTGGCCGAACCTGCACGCCTGCCGGCAACTAGTCGGCA ATCCCCAAGCGACCTTCGAGGACGTGGTGAAGCATCCGGCTGTCATCGATTGCGTCCGTCGCGGACTTCAGGCGCACAAC AAGGAGTGCGAGGGCGCCAGCAGCCGGCGCATCGGCCGCGCGATGCTGATGGTCGAGCCGCCGTCGATCGACGGCCACGA GCTGACCGACAAGGGCTACATCAACCAGCGCGCCGGCCTGGAACGCCGCGCCGCCCTGGTCGAGCGTCTCTATGCTGAAA CGCCCGACAGCGAGGTGATCGTGCTGTGA
Upstream 100 bases:
>100_bases TCAAGGAAGGCATTGAAGCCTTCCTCCAGAAGCGCAAGCCGGTCTATACCAACCGCTAGATATCAAGATCCGCAAAGGAC GATATTTCGGGAGACGCAAA
Downstream 100 bases:
>100_bases TGCTGTCATTGCCAGTCGCTCGGTGTCGTCCCCGCGAACGCGGGGACCCATACTCCCAGGCTCATCCGTATGCCGCCGAA GCCCGTTCCGGGCCCATCGA
Product: acyl-CoA synthetase
Products: NA
Alternate protein names: Long-chain acyl-CoA synthetase; Acyl-CoA synthetase [H]
Number of amino acids: Translated: 622; Mature: 622
Protein sequence:
>622_residues MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIVIKSRIPLQAYEPHLAAPLARWAKEAPTRTWLAQRSGPERQWRKLP YAEAKRTIDGLTQGLLNLKLDGRPVAILSGNSIEHALMTFAAMQARSPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMV QDGPTFEKALKALDLDGVTVVHVARPCEGIPSVSFADLAATPVTGDVDASIAQITPETVGKLLFTSGSTGMPKAVINTQR MMCANAAMMMQTRPRAPDAPVSTVLDWMPWNHTMGGNAAFNPLLIDGGTLYIDDGRPMPGMFDETIKNLREVSPTYYANV PAGYAALAAAMEKDDALCRSFFKDLGIMAYGGARLPDDLYERMQALAVKTTGERIVFYTGWGSTETAPTSTGTYWNTERV GLIGLPFPGVELKMVPTGDKYELRLRGINVMPGYFGQPELTRKAFDDEGFYCIGDAGVFVDPNDPAQGIIFAGRVVEDFK LTTGTFVHVGSLRTDAIAAATPAIHDALVAGQDREYIGLLAWPNLHACRQLVGNPQATFEDVVKHPAVIDCVRRGLQAHN KECEGASSRRIGRAMLMVEPPSIDGHELTDKGYINQRAGLERRAALVERLYAETPDSEVIVL
Sequences:
>Translated_622_residues MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIVIKSRIPLQAYEPHLAAPLARWAKEAPTRTWLAQRSGPERQWRKLP YAEAKRTIDGLTQGLLNLKLDGRPVAILSGNSIEHALMTFAAMQARSPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMV QDGPTFEKALKALDLDGVTVVHVARPCEGIPSVSFADLAATPVTGDVDASIAQITPETVGKLLFTSGSTGMPKAVINTQR MMCANAAMMMQTRPRAPDAPVSTVLDWMPWNHTMGGNAAFNPLLIDGGTLYIDDGRPMPGMFDETIKNLREVSPTYYANV PAGYAALAAAMEKDDALCRSFFKDLGIMAYGGARLPDDLYERMQALAVKTTGERIVFYTGWGSTETAPTSTGTYWNTERV GLIGLPFPGVELKMVPTGDKYELRLRGINVMPGYFGQPELTRKAFDDEGFYCIGDAGVFVDPNDPAQGIIFAGRVVEDFK LTTGTFVHVGSLRTDAIAAATPAIHDALVAGQDREYIGLLAWPNLHACRQLVGNPQATFEDVVKHPAVIDCVRRGLQAHN KECEGASSRRIGRAMLMVEPPSIDGHELTDKGYINQRAGLERRAALVERLYAETPDSEVIVL >Mature_622_residues MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIVIKSRIPLQAYEPHLAAPLARWAKEAPTRTWLAQRSGPERQWRKLP YAEAKRTIDGLTQGLLNLKLDGRPVAILSGNSIEHALMTFAAMQARSPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMV QDGPTFEKALKALDLDGVTVVHVARPCEGIPSVSFADLAATPVTGDVDASIAQITPETVGKLLFTSGSTGMPKAVINTQR MMCANAAMMMQTRPRAPDAPVSTVLDWMPWNHTMGGNAAFNPLLIDGGTLYIDDGRPMPGMFDETIKNLREVSPTYYANV PAGYAALAAAMEKDDALCRSFFKDLGIMAYGGARLPDDLYERMQALAVKTTGERIVFYTGWGSTETAPTSTGTYWNTERV GLIGLPFPGVELKMVPTGDKYELRLRGINVMPGYFGQPELTRKAFDDEGFYCIGDAGVFVDPNDPAQGIIFAGRVVEDFK LTTGTFVHVGSLRTDAIAAATPAIHDALVAGQDREYIGLLAWPNLHACRQLVGNPQATFEDVVKHPAVIDCVRRGLQAHN KECEGASSRRIGRAMLMVEPPSIDGHELTDKGYINQRAGLERRAALVERLYAETPDSEVIVL
Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1788107, Length=284, Percent_Identity=28.5211267605634, Blast_Score=72, Evalue=1e-13, Organism=Escherichia coli, GI145693145, Length=350, Percent_Identity=26, Blast_Score=71, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17564090, Length=249, Percent_Identity=27.710843373494, Blast_Score=67, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR020845 - InterPro: IPR000873 [H]
Pfam domain/function: PF00501 AMP-binding [H]
EC number: =6.2.1.3 [H]
Molecular weight: Translated: 67762; Mature: 67762
Theoretical pI: Translated: 6.65; Mature: 6.65
Prosite motif: PS00455 AMP_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 3.5 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIVIKSRIPLQAYEPHLAAPLARWAKEA CCCHHHHHHHCCCHHHHHHHHHHCCHHHCCCCEEEEECCCCCHHCCCHHHHHHHHHHHHC PTRTWLAQRSGPERQWRKLPYAEAKRTIDGLTQGLLNLKLDGRPVAILSGNSIEHALMTF CCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCHHHHHHHH AAMQARSPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMVQDGPTFEKALKALDLDGVTV HHHHHCCCCCCCCHHHHHHCCCCEEHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCEEE VHVARPCEGIPSVSFADLAATPVTGDVDASIAQITPETVGKLLFTSGSTGMPKAVINTQR EEEECCCCCCCCCCHHHHCCCCCCCCCCCHHHHCCHHHHHHEEEECCCCCCCHHHHHHHH MMCANAAMMMQTRPRAPDAPVSTVLDWMPWNHTMGGNAAFNPLLIDGGTLYIDDGRPMPG HHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCC MFDETIKNLREVSPTYYANVPAGYAALAAAMEKDDALCRSFFKDLGIMAYGGARLPDDLY HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHH ERMQALAVKTTGERIVFYTGWGSTETAPTSTGTYWNTERVGLIGLPFPGVELKMVPTGDK HHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCEECCCCEEEEECCCCCCEEEEECCCCC YELRLRGINVMPGYFGQPELTRKAFDDEGFYCIGDAGVFVDPNDPAQGIIFAGRVVEDFK EEEEEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCEEEECCCCCCCCEEEECEEEECEE LTTGTFVHVGSLRTDAIAAATPAIHDALVAGQDREYIGLLAWPNLHACRQLVGNPQATFE ECCCCEEEECCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCHHHH DVVKHPAVIDCVRRGLQAHNKECEGASSRRIGRAMLMVEPPSIDGHELTDKGYINQRAGL HHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCCCCHHCCH ERRAALVERLYAETPDSEVIVL HHHHHHHHHHHHCCCCCCEEEC >Mature Secondary Structure MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIVIKSRIPLQAYEPHLAAPLARWAKEA CCCHHHHHHHCCCHHHHHHHHHHCCHHHCCCCEEEEECCCCCHHCCCHHHHHHHHHHHHC PTRTWLAQRSGPERQWRKLPYAEAKRTIDGLTQGLLNLKLDGRPVAILSGNSIEHALMTF CCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCHHHHHHHH AAMQARSPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMVQDGPTFEKALKALDLDGVTV HHHHHCCCCCCCCHHHHHHCCCCEEHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCEEE VHVARPCEGIPSVSFADLAATPVTGDVDASIAQITPETVGKLLFTSGSTGMPKAVINTQR EEEECCCCCCCCCCHHHHCCCCCCCCCCCHHHHCCHHHHHHEEEECCCCCCCHHHHHHHH MMCANAAMMMQTRPRAPDAPVSTVLDWMPWNHTMGGNAAFNPLLIDGGTLYIDDGRPMPG HHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCC MFDETIKNLREVSPTYYANVPAGYAALAAAMEKDDALCRSFFKDLGIMAYGGARLPDDLY HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHH ERMQALAVKTTGERIVFYTGWGSTETAPTSTGTYWNTERVGLIGLPFPGVELKMVPTGDK HHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCEECCCCEEEEECCCCCCEEEEECCCCC YELRLRGINVMPGYFGQPELTRKAFDDEGFYCIGDAGVFVDPNDPAQGIIFAGRVVEDFK EEEEEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCEEEECCCCCCCCEEEECEEEECEE LTTGTFVHVGSLRTDAIAAATPAIHDALVAGQDREYIGLLAWPNLHACRQLVGNPQATFE ECCCCEEEECCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCHHHH DVVKHPAVIDCVRRGLQAHNKECEGASSRRIGRAMLMVEPPSIDGHELTDKGYINQRAGL HHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCCCCHHCCH ERRAALVERLYAETPDSEVIVL HHHHHHHHHHHHCCCCCCEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]