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The map label for this gene is echA12 [H]

Identifier: 146338039

GI number: 146338039

Start: 995632

End: 996462

Strand: Direct

Name: echA12 [H]

Synonym: BRADO0932

Alternate gene names: 146338039

Gene position: 995632-996462 (Clockwise)

Preceding gene: 146338038

Following gene: 146338040

Centisome position: 13.35

GC content: 65.22

Gene sequence:

>831_bases
ATGAAGGTGAGGAGAGAGCGCGCCATGTCCCAACCGCTGCTGATCGAGCATGAGAACGGCGTCGACCGGGTGACGCTCAA
TCGTCCGGAGAGCCTGAACGCGCTCGATCCGGCGCTGATCGACGCGCTCAATGCTTATTTTGAAAGCCTGCAGCGAAACC
GCGCGACGCGCGTCGTCGTGCTCAAGGGCGCGGGCAAGAACTTCTGCGCCGGCCTCGATCTCAAGGCCGCCATGCAGCGC
CGCGGCGGCGTCAACGAGCCGCCCACTGTGACCGAATCGCTCGACTCGCAGCGCCGCATCGCCGACATCGTCATGCTGAT
GCGGCGCTGTCCGCAGCCGATCATCGCGCTTGTGCAGGGCGCGGCCGCGGGCGGCGGCTTCGCGCTGGCGCTCGCCGCCG
ACATCCGCATCGCCGCACGCTCGGCGCGCATGAACTGCGCTTTCATCAAGCTCGGCCTCGGGGGCTGCGACATCGGCACC
AGCTACTTTTTGCCGCGCCTCGTCGGCGTCTCCGTCGCCTCCGAGCTGATTCTCACCGGACGCTTCATCGGCGCCGAACG
CGCGCTGATGGTCGGGCTGGTGTCGGAGATCGTCGAAGAGGATCAGCTCGATGATGCCGCCGCGCCGATCATCGATGCGA
TGATGACGGCCTCCCCAGTCGGGCTTCGCCTGTCGAAGGAATGCCTCAACATGAGCGTCGATGCGTCGTCGATCGAGGCC
GTCATCGCGATGGAAGATCGCAACCAGGTGCTGTGCAGCCGCTCGGAAGACTTCAAGGAAGGCATTGAAGCCTTCCTCCA
GAAGCGCAAGCCGGTCTATACCAACCGCTAG

Upstream 100 bases:

>100_bases
AGCCGGGACCCAGACTCCGCGGCGCCTGTGAGTGGCAAACTGGCAGACGCCGCGCTCTAACCAATGAAGCCGGTGGCTAT
GGATCCCGGCGTTCGCCGGG

Downstream 100 bases:

>100_bases
ATATCAAGATCCGCAAAGGACGATATTTCGGGAGACGCAAAATGGACGGGACGGCAGCGAAGGTGTTGGAGAAGCCGGCA
TTCCGCAAGGTCAACTGGCT

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKVRRERAMSQPLLIEHENGVDRVTLNRPESLNALDPALIDALNAYFESLQRNRATRVVVLKGAGKNFCAGLDLKAAMQR
RGGVNEPPTVTESLDSQRRIADIVMLMRRCPQPIIALVQGAAAGGGFALALAADIRIAARSARMNCAFIKLGLGGCDIGT
SYFLPRLVGVSVASELILTGRFIGAERALMVGLVSEIVEEDQLDDAAAPIIDAMMTASPVGLRLSKECLNMSVDASSIEA
VIAMEDRNQVLCSRSEDFKEGIEAFLQKRKPVYTNR

Sequences:

>Translated_276_residues
MKVRRERAMSQPLLIEHENGVDRVTLNRPESLNALDPALIDALNAYFESLQRNRATRVVVLKGAGKNFCAGLDLKAAMQR
RGGVNEPPTVTESLDSQRRIADIVMLMRRCPQPIIALVQGAAAGGGFALALAADIRIAARSARMNCAFIKLGLGGCDIGT
SYFLPRLVGVSVASELILTGRFIGAERALMVGLVSEIVEEDQLDDAAAPIIDAMMTASPVGLRLSKECLNMSVDASSIEA
VIAMEDRNQVLCSRSEDFKEGIEAFLQKRKPVYTNR
>Mature_276_residues
MKVRRERAMSQPLLIEHENGVDRVTLNRPESLNALDPALIDALNAYFESLQRNRATRVVVLKGAGKNFCAGLDLKAAMQR
RGGVNEPPTVTESLDSQRRIADIVMLMRRCPQPIIALVQGAAAGGGFALALAADIRIAARSARMNCAFIKLGLGGCDIGT
SYFLPRLVGVSVASELILTGRFIGAERALMVGLVSEIVEEDQLDDAAAPIIDAMMTASPVGLRLSKECLNMSVDASSIEA
VIAMEDRNQVLCSRSEDFKEGIEAFLQKRKPVYTNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=260, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=30.8880308880309, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4502327, Length=267, Percent_Identity=31.4606741573034, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI31542718, Length=271, Percent_Identity=25.830258302583, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=26.8867924528302, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=26.8867924528302, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=30.6878306878307, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI260275230, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI260274832, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=254, Percent_Identity=26.7716535433071, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=35.8208955223881, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=32.1969696969697, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=32.4817518248175, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=31.2741312741313, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=29.0566037735849, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=28.7179487179487, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=31.9148936170213, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=263, Percent_Identity=31.1787072243346, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=29.3859649122807, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=33.4645669291339, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=30.8016877637131, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=29.7619047619048, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=28.1481481481481, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=31.0502283105023, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=27.7992277992278, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=264, Percent_Identity=30.3030303030303, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=30.8080808080808, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=30.8080808080808, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=26.984126984127, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=200, Percent_Identity=26, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=33.2075471698113, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=33.984375, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=33.984375, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920382, Length=266, Percent_Identity=31.5789473684211, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922422, Length=266, Percent_Identity=25.5639097744361, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=285, Percent_Identity=27.719298245614, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571730, Length=251, Percent_Identity=25.4980079681275, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571729, Length=251, Percent_Identity=25.4980079681275, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24650670, Length=249, Percent_Identity=27.3092369477912, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24654903, Length=202, Percent_Identity=24.7524752475248, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29901; Mature: 29901

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRRERAMSQPLLIEHENGVDRVTLNRPESLNALDPALIDALNAYFESLQRNRATRVVV
CCCCHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
LKGAGKNFCAGLDLKAAMQRRGGVNEPPTVTESLDSQRRIADIVMLMRRCPQPIIALVQG
EECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
AAAGGGFALALAADIRIAARSARMNCAFIKLGLGGCDIGTSYFLPRLVGVSVASELILTG
CCCCCCEEEEEHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RFIGAERALMVGLVSEIVEEDQLDDAAAPIIDAMMTASPVGLRLSKECLNMSVDASSIEA
HHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHEE
VIAMEDRNQVLCSRSEDFKEGIEAFLQKRKPVYTNR
EEEECCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKVRRERAMSQPLLIEHENGVDRVTLNRPESLNALDPALIDALNAYFESLQRNRATRVVV
CCCCHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
LKGAGKNFCAGLDLKAAMQRRGGVNEPPTVTESLDSQRRIADIVMLMRRCPQPIIALVQG
EECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
AAAGGGFALALAADIRIAARSARMNCAFIKLGLGGCDIGTSYFLPRLVGVSVASELILTG
CCCCCCEEEEEHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RFIGAERALMVGLVSEIVEEDQLDDAAAPIIDAMMTASPVGLRLSKECLNMSVDASSIEA
HHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHEE
VIAMEDRNQVLCSRSEDFKEGIEAFLQKRKPVYTNR
EEEECCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]