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The map label for this gene is gcvA [H]

Identifier: 146338022

GI number: 146338022

Start: 977128

End: 978048

Strand: Reverse

Name: gcvA [H]

Synonym: BRADO0915

Alternate gene names: 146338022

Gene position: 978048-977128 (Counterclockwise)

Preceding gene: 146338025

Following gene: 146338021

Centisome position: 13.12

GC content: 67.32

Gene sequence:

>921_bases
ATGGCGCGGCTACCGTCGCTGAATGGGTTGCGGGCGTTCGAGGCCGCGGCGCGGCATCTGAGCTTCACCCGCGCCGCCTC
CGAGCTGAACGTCACGCAGACGGCCATCAGCCATCAGATCAAGCGCCTGGAGGACGAGCTCGGCATCCGCCTGTTCCTGC
GCCAGAACCGCAGCCTCGCGCTGACGCCGCAGGCCCGCGACTATCTTCCGCTGGTGCGGGCCGCCTTCGACGATCTGCGC
TTGGCGACCGAGCGGCTGGTGCGGAAGGATAACGCCAAGGTGCTGACCGTCTCCACCATCGCCTCGCTCGCCGCCAAATG
GCTGCTGCCGCGGCTGACGGCTTTCCAGGAAGCTCATCCCACCATCGACGTCCGCATCACCACCTCGACCGGGCTGGTCG
ATTTCCGCAAGGACGATGTCGACGCCGCCATTCGCTACGGCCGCGGCCACTGGCCAGCGGTGCGTGCGGAATGGCTGATG
GCTGATGAGCTGTTTCCGGTGTGCAGCCCGGCGCTGCTGCAGGGAACCAAGCCGCTGCGCAGCCCCGAGGACCTCGCGCA
TCACACGCTGCTGCAGGCGAGCGGTGGCTATGACGACGACTGGCGGCTCTGGCTGACGGCAGCGGGCCTGCCTGTCGAGA
TGTCCCGGCAGAGCCGGCTGAGCTTCGACCTGTCGCTGATGACCGTGCAGGCGGCGATCGACGGACTCGGCGTCGCGATC
GGGCGCACGGCCTACGTCCAGGACGATATCGCCAAGGGGCGCCTCGTGGTTCCGTTCGAGATGATGCTCCCGAGCGACGC
CGGCTTCTATCTGGTCACGCCGGAGGCGCGCACCGATCCGCCGAAGCTCAAGGCGTTTCGGCAATGGCTCAAGAGTGCGG
CGCAGCAGAAGAACGTCTCGTTGAATCCCCTTCCGCCTTAA

Upstream 100 bases:

>100_bases
ATAAAGCGTGAAGCTGCAATATCGAGGGCTTGCCTTTCATCTACAAACGACATTTATTACCGTTTCGGATGAAATAAGCT
CATGAATGAAGGAGACGCGG

Downstream 100 bases:

>100_bases
GGATTTGGCGTCAAATCTATTGGCCGGCACGATGTCGCGTGGCAGATTGCCACACCCTTTCTCATCGGCCGGCGCAGAAG
TTCTTCCGCACGAGCCACTC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MARLPSLNGLRAFEAAARHLSFTRAASELNVTQTAISHQIKRLEDELGIRLFLRQNRSLALTPQARDYLPLVRAAFDDLR
LATERLVRKDNAKVLTVSTIASLAAKWLLPRLTAFQEAHPTIDVRITTSTGLVDFRKDDVDAAIRYGRGHWPAVRAEWLM
ADELFPVCSPALLQGTKPLRSPEDLAHHTLLQASGGYDDDWRLWLTAAGLPVEMSRQSRLSFDLSLMTVQAAIDGLGVAI
GRTAYVQDDIAKGRLVVPFEMMLPSDAGFYLVTPEARTDPPKLKAFRQWLKSAAQQKNVSLNPLPP

Sequences:

>Translated_306_residues
MARLPSLNGLRAFEAAARHLSFTRAASELNVTQTAISHQIKRLEDELGIRLFLRQNRSLALTPQARDYLPLVRAAFDDLR
LATERLVRKDNAKVLTVSTIASLAAKWLLPRLTAFQEAHPTIDVRITTSTGLVDFRKDDVDAAIRYGRGHWPAVRAEWLM
ADELFPVCSPALLQGTKPLRSPEDLAHHTLLQASGGYDDDWRLWLTAAGLPVEMSRQSRLSFDLSLMTVQAAIDGLGVAI
GRTAYVQDDIAKGRLVVPFEMMLPSDAGFYLVTPEARTDPPKLKAFRQWLKSAAQQKNVSLNPLPP
>Mature_305_residues
ARLPSLNGLRAFEAAARHLSFTRAASELNVTQTAISHQIKRLEDELGIRLFLRQNRSLALTPQARDYLPLVRAAFDDLRL
ATERLVRKDNAKVLTVSTIASLAAKWLLPRLTAFQEAHPTIDVRITTSTGLVDFRKDDVDAAIRYGRGHWPAVRAEWLMA
DELFPVCSPALLQGTKPLRSPEDLAHHTLLQASGGYDDDWRLWLTAAGLPVEMSRQSRLSFDLSLMTVQAAIDGLGVAIG
RTAYVQDDIAKGRLVVPFEMMLPSDAGFYLVTPEARTDPPKLKAFRQWLKSAAQQKNVSLNPLPP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=49.6644295302013, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=37.2881355932203, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=32.5423728813559, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.125, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=25.3289473684211, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=24.9134948096886, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789639, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=25.4098360655738, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34027; Mature: 33895

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLPSLNGLRAFEAAARHLSFTRAASELNVTQTAISHQIKRLEDELGIRLFLRQNRSLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEE
LTPQARDYLPLVRAAFDDLRLATERLVRKDNAKVLTVSTIASLAAKWLLPRLTAFQEAHP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
TIDVRITTSTGLVDFRKDDVDAAIRYGRGHWPAVRAEWLMADELFPVCSPALLQGTKPLR
EEEEEEECCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCC
SPEDLAHHTLLQASGGYDDDWRLWLTAAGLPVEMSRQSRLSFDLSLMTVQAAIDGLGVAI
CHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEECCCCCCCEEHHHHHHHHHHHHHHHHHH
GRTAYVQDDIAKGRLVVPFEMMLPSDAGFYLVTPEARTDPPKLKAFRQWLKSAAQQKNVS
CCCEEECHHHHCCEEEEEEHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
LNPLPP
CCCCCC
>Mature Secondary Structure 
ARLPSLNGLRAFEAAARHLSFTRAASELNVTQTAISHQIKRLEDELGIRLFLRQNRSLA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEE
LTPQARDYLPLVRAAFDDLRLATERLVRKDNAKVLTVSTIASLAAKWLLPRLTAFQEAHP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
TIDVRITTSTGLVDFRKDDVDAAIRYGRGHWPAVRAEWLMADELFPVCSPALLQGTKPLR
EEEEEEECCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCC
SPEDLAHHTLLQASGGYDDDWRLWLTAAGLPVEMSRQSRLSFDLSLMTVQAAIDGLGVAI
CHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEECCCCCCCEEHHHHHHHHHHHHHHHHHH
GRTAYVQDDIAKGRLVVPFEMMLPSDAGFYLVTPEARTDPPKLKAFRQWLKSAAQQKNVS
CCCEEECHHHHCCEEEEEEHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
LNPLPP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]