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The map label for this gene is citA [H]

Identifier: 146338021

GI number: 146338021

Start: 975694

End: 977007

Strand: Reverse

Name: citA [H]

Synonym: BRADO0914

Alternate gene names: 146338021

Gene position: 977007-975694 (Counterclockwise)

Preceding gene: 146338022

Following gene: 146338020

Centisome position: 13.1

GC content: 65.68

Gene sequence:

>1314_bases
GTGTCATCGACCCCCATCCAGCAGCCGGCCACCATCAAGTCGCGCATCGGCGCGATCCTGCGCGCCACCAGCGGCAATTT
CCTCGAGCAGTTCGACTTCTTCCTGTTCGGATTCTATGCGGCCGCGATCGGCAAGGCGTTCTTCCCGTCCACCGACGAGA
CGGCGTCGCTGCTCAACACGTTCGGCGTGTTCTGGCTCGGCGCGCTGATGCGCCCGGTCGGAGCGATCGTGCTCGGCGCC
TATATCGACAAGATCGGCCGCCGCCAGGGCCTGATCGTGACGCTTGGCATCATGGCCGCCGGCACTGTCGTGATCGCGTT
CTGCCCGACCTATGAGACCATTGGCGTCGCTGCGCCGATCATCGTCCTGCTCGGCCGCCTGCTGCAAGGCTTCTCGGCCG
GCGTCGAGCTCGGCGGCGTGTCGGTCTATCTGTCGGAGATCGCCACGCCCGGCAATCGCGGCTTCTACACCTCGTTCCAA
TCGTCGAGCCAGCAGGTCGCGATCTTCGTGGCCTCCATTCTCGGCTATCTCCTGAGCGAATCCATGCCGGCGGCGACCGT
GACCGCGTGGGGCTGGCGCATCCCGTTCTTCGTCGGCTGCCTGATCATTCCGCTGATCTTCTTCCTGCGCCGCACGCTGG
AGGAGACGCCGGCGTTCCTGGCGATGAAGAAGCACCCGACCACCAGCGAGGTATTCGCCTCGGCACTCGCCAACTGGCGC
ATCGTGCTGCTCGGCATGATGATCGCGATCCTGACCACGACGACGTTCTACTTCGTCACCGTCTACACGCCGACCTTCGG
CAAGACCGTGCTGAAGCTCTCGACCGCCGATGCACTGCTTGTGACGCTGCTGGTCGCCGTCACCAACTTCATCTGGAACC
CGGTCGGCGGCGCGCTGTCCGACCGTATCGGCCGCAAGCCGGTACTGATCGGAATCGCCGTGCTGTCACTGCTGACGGCC
TATCCGGCGCTGAGCTGGCTGGTGTCGGCACCGACCTTCGGCAAGATGCTGGCGGTCGAGATGATGTTCTCGTTCTATTT
CGGCATGTACAGCGGCACCATGCTCGGCGCGCTGGTCGAGATCGTGCCGGCGCATGTCCGCACCACCTGCTTCTCGCTGG
CCTTCGCGCTCGCAGCCGCACTGTTCGGTACCTTCACGCCGTTCGCAGCGACCTGGCTGATCGAGAAGACCGGCGACAAG
GCCTCGCCCGGCTTCTGGCTGATGTTCGCCGCCGCGCTCGGCATCATCGCCGCGCTGACCGTCTATCGCGGCGGCCAGCA
GGCGGTGGCGGCGCGCGAGCCTGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGCCGGCACGATGTCGCGTGGCAGATTGCCACACCCTTTCTCATCGGCCGGCGCAGAAGTTCTTCCGCACGAGCCACTC
GACGGTAGGAGTTTTCTCGC

Downstream 100 bases:

>100_bases
TCGGCTTGTTTGTTGACGCCAAGGGCGGCATCATCCGATGCCGCCCTTTTTGTTTGCGGCCGGAGCGGGAGCAAGGTCTG
TGATGGATGAACTGATTGTC

Product: citrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSSTPIQQPATIKSRIGAILRATSGNFLEQFDFFLFGFYAAAIGKAFFPSTDETASLLNTFGVFWLGALMRPVGAIVLGA
YIDKIGRRQGLIVTLGIMAAGTVVIAFCPTYETIGVAAPIIVLLGRLLQGFSAGVELGGVSVYLSEIATPGNRGFYTSFQ
SSSQQVAIFVASILGYLLSESMPAATVTAWGWRIPFFVGCLIIPLIFFLRRTLEETPAFLAMKKHPTTSEVFASALANWR
IVLLGMMIAILTTTTFYFVTVYTPTFGKTVLKLSTADALLVTLLVAVTNFIWNPVGGALSDRIGRKPVLIGIAVLSLLTA
YPALSWLVSAPTFGKMLAVEMMFSFYFGMYSGTMLGALVEIVPAHVRTTCFSLAFALAAALFGTFTPFAATWLIEKTGDK
ASPGFWLMFAAALGIIAALTVYRGGQQAVAAREPAAA

Sequences:

>Translated_437_residues
MSSTPIQQPATIKSRIGAILRATSGNFLEQFDFFLFGFYAAAIGKAFFPSTDETASLLNTFGVFWLGALMRPVGAIVLGA
YIDKIGRRQGLIVTLGIMAAGTVVIAFCPTYETIGVAAPIIVLLGRLLQGFSAGVELGGVSVYLSEIATPGNRGFYTSFQ
SSSQQVAIFVASILGYLLSESMPAATVTAWGWRIPFFVGCLIIPLIFFLRRTLEETPAFLAMKKHPTTSEVFASALANWR
IVLLGMMIAILTTTTFYFVTVYTPTFGKTVLKLSTADALLVTLLVAVTNFIWNPVGGALSDRIGRKPVLIGIAVLSLLTA
YPALSWLVSAPTFGKMLAVEMMFSFYFGMYSGTMLGALVEIVPAHVRTTCFSLAFALAAALFGTFTPFAATWLIEKTGDK
ASPGFWLMFAAALGIIAALTVYRGGQQAVAAREPAAA
>Mature_436_residues
SSTPIQQPATIKSRIGAILRATSGNFLEQFDFFLFGFYAAAIGKAFFPSTDETASLLNTFGVFWLGALMRPVGAIVLGAY
IDKIGRRQGLIVTLGIMAAGTVVIAFCPTYETIGVAAPIIVLLGRLLQGFSAGVELGGVSVYLSEIATPGNRGFYTSFQS
SSQQVAIFVASILGYLLSESMPAATVTAWGWRIPFFVGCLIIPLIFFLRRTLEETPAFLAMKKHPTTSEVFASALANWRI
VLLGMMIAILTTTTFYFVTVYTPTFGKTVLKLSTADALLVTLLVAVTNFIWNPVGGALSDRIGRKPVLIGIAVLSLLTAY
PALSWLVSAPTFGKMLAVEMMFSFYFGMYSGTMLGALVEIVPAHVRTTCFSLAFALAAALFGTFTPFAATWLIEKTGDKA
SPGFWLMFAAALGIIAALTVYRGGQQAVAAREPAAA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=32.183908045977, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788942, Length=427, Percent_Identity=33.9578454332553, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=30.4651162790698, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788292, Length=403, Percent_Identity=26.7990074441687, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=29.5238095238095, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46714; Mature: 46582

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPIQQPATIKSRIGAILRATSGNFLEQFDFFLFGFYAAAIGKAFFPSTDETASLLNT
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FGVFWLGALMRPVGAIVLGAYIDKIGRRQGLIVTLGIMAAGTVVIAFCPTYETIGVAAPI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCEEEEECCCHHHHHHHHHH
IVLLGRLLQGFSAGVELGGVSVYLSEIATPGNRGFYTSFQSSSQQVAIFVASILGYLLSE
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHC
SMPAATVTAWGWRIPFFVGCLIIPLIFFLRRTLEETPAFLAMKKHPTTSEVFASALANWR
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHH
IVLLGMMIAILTTTTFYFVTVYTPTFGKTVLKLSTADALLVTLLVAVTNFIWNPVGGALS
HHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DRIGRKPVLIGIAVLSLLTAYPALSWLVSAPTFGKMLAVEMMFSFYFGMYSGTMLGALVE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVPAHVRTTCFSLAFALAAALFGTFTPFAATWLIEKTGDKASPGFWLMFAAALGIIAALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
VYRGGQQAVAAREPAAA
HHCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SSTPIQQPATIKSRIGAILRATSGNFLEQFDFFLFGFYAAAIGKAFFPSTDETASLLNT
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FGVFWLGALMRPVGAIVLGAYIDKIGRRQGLIVTLGIMAAGTVVIAFCPTYETIGVAAPI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCEEEEECCCHHHHHHHHHH
IVLLGRLLQGFSAGVELGGVSVYLSEIATPGNRGFYTSFQSSSQQVAIFVASILGYLLSE
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHC
SMPAATVTAWGWRIPFFVGCLIIPLIFFLRRTLEETPAFLAMKKHPTTSEVFASALANWR
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHH
IVLLGMMIAILTTTTFYFVTVYTPTFGKTVLKLSTADALLVTLLVAVTNFIWNPVGGALS
HHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DRIGRKPVLIGIAVLSLLTAYPALSWLVSAPTFGKMLAVEMMFSFYFGMYSGTMLGALVE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVPAHVRTTCFSLAFALAAALFGTFTPFAATWLIEKTGDKASPGFWLMFAAALGIIAALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
VYRGGQQAVAAREPAAA
HHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]