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The map label for this gene is cca [H]

Identifier: 146302633

GI number: 146302633

Start: 5928627

End: 5930114

Strand: Direct

Name: cca [H]

Synonym: Fjoh_4906

Alternate gene names: 146302633

Gene position: 5928627-5930114 (Clockwise)

Preceding gene: 146302631

Following gene: 146302634

Centisome position: 97.24

GC content: 35.35

Gene sequence:

>1488_bases
ATGCATTTTATTTCTACTGAAATTAGAAATGCTTTTTATTCAAAAACTGAATTCGTGGCGATACAAACAAATTATAAAAC
TGCTTTACAAAACAAAATCTTCGATGTTATTTCGAAAGCTTCTCAGGAATTAAAAGTTGACTCTTATGTGATAGGAGGTT
TTGTTCGTGATTTGCTTTTAAATCGAGGTTCTAAAAAAGACATTGATGTTGTTGCAGTTGGAAGCGGCATCGAATTAGCT
CTTAAAGTTTCAGATTTACTTCCTAACAAACCAAAAGTTCAGGTTTTTAAAACTTATGGAACAGCGATGTTACGTTTTGA
AGATACTGATATCGAGTTTGTCGGTGCCCGAAAAGAATCTTATACCCGAGACAGCCGAAATCCGATTGTCGAAAACGGAA
CTTTACAAGACGATCAAAATCGTCGTGATTTTACGATCAATGCATTGGCTTTATCTTTAAATTCAACTAATTTCGGAGAT
CTTCTGGATCCGTTTGACGGTTTAACCGATTTAGAAAATAAAACAATCAAAACGCCTTTGGATCCAGATATTACGTATTC
TGATGATCCTTTGCGAATGCTTCGCGCCATTCGTTTTGCAACGCAGTTGAATTTTGAAATTGAAGAAAATTCATTGAATG
CCATCACAAAAAATGCTGATCGCATCAAAATAATTTCCGGTGAAAGAATAGTTGATGAATTAAACAAAATTCTTTCTACA
CCAAAACCTTCATCCGGATTTTTACTTTTATACAAAACTGGACTTTTAGATTTAATATTACCTGAATTAACGGCGTTGAA
TCAGGTAGAAGAAATTGAAGGCCATACGCATAAAAACAATTTTTATCATACACTTGAAGTTGTTGACAACATCTGTCCGA
ACACAGACGACGTTTGGCTTCGCTGGTCGGCATTACTGCATGATATTGGAAAAGCACCAACGAAACGTTTTACAAAAAAA
CAAGGATGGACTTTTCACGGACATGAATTTTTGGGCGGAAAAATGGCGAAGAAAATTTTCGAACGTTTGCACATGCCTTT
GAACCATAAAATGAAATTCGTTCAAAAAATGGTTATCATGAGTTCACGTCCCATTGTTTTGGCGCAGGATATTGTGACCG
ACAGTGCAGTTCGCCGTTTGGTTTTTGATGCTGGTGAAGATGTAGAAAACCTAATGACTTTGTGTGAAGCAGATATCACA
ACCAAAAATCCGTCAAAATTCAAGAAATATCATAAAAATTTCGAAATCGTCCGCAAGAAAATTGTAGAAGTCGAAGAACG
CGATCATGTACGTAATTTTCAGCCGCCTATTTCCGGCGAAGAAATTATGGAAATATTCGATTTAAAACCTTCACGAGAAA
TCGGAATTTTGAAAGAAGCAGTGAAGGAAGCAATTTTAGAAGGTGATATTCCAAATGAATATCAAGCTGCTTATGATTTT
GTGATTAAAAGAGCAGCGAAATTAGGCTTAAAAAAAGTTGAGAAATAA

Upstream 100 bases:

>100_bases
AACCTTTCCCAATTTATAAAAATCGACGCTGCAAAATGTGAAATTGTTCAAAAAAGTGAGCGATAGATTATAATAGGCAT
TTTCTATGTTGTAAATTTGC

Downstream 100 bases:

>100_bases
GTAGTATTTAATAAAATGAAAAAAGAGAATAAATCAGTAATCATTTGGTTACTATCAGGCTGTGTTTTATTGTTTTTAAT
GGTTGTTGTGGGAGGAATTA

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MHFISTEIRNAFYSKTEFVAIQTNYKTALQNKIFDVISKASQELKVDSYVIGGFVRDLLLNRGSKKDIDVVAVGSGIELA
LKVSDLLPNKPKVQVFKTYGTAMLRFEDTDIEFVGARKESYTRDSRNPIVENGTLQDDQNRRDFTINALALSLNSTNFGD
LLDPFDGLTDLENKTIKTPLDPDITYSDDPLRMLRAIRFATQLNFEIEENSLNAITKNADRIKIISGERIVDELNKILST
PKPSSGFLLLYKTGLLDLILPELTALNQVEEIEGHTHKNNFYHTLEVVDNICPNTDDVWLRWSALLHDIGKAPTKRFTKK
QGWTFHGHEFLGGKMAKKIFERLHMPLNHKMKFVQKMVIMSSRPIVLAQDIVTDSAVRRLVFDAGEDVENLMTLCEADIT
TKNPSKFKKYHKNFEIVRKKIVEVEERDHVRNFQPPISGEEIMEIFDLKPSREIGILKEAVKEAILEGDIPNEYQAAYDF
VIKRAAKLGLKKVEK

Sequences:

>Translated_495_residues
MHFISTEIRNAFYSKTEFVAIQTNYKTALQNKIFDVISKASQELKVDSYVIGGFVRDLLLNRGSKKDIDVVAVGSGIELA
LKVSDLLPNKPKVQVFKTYGTAMLRFEDTDIEFVGARKESYTRDSRNPIVENGTLQDDQNRRDFTINALALSLNSTNFGD
LLDPFDGLTDLENKTIKTPLDPDITYSDDPLRMLRAIRFATQLNFEIEENSLNAITKNADRIKIISGERIVDELNKILST
PKPSSGFLLLYKTGLLDLILPELTALNQVEEIEGHTHKNNFYHTLEVVDNICPNTDDVWLRWSALLHDIGKAPTKRFTKK
QGWTFHGHEFLGGKMAKKIFERLHMPLNHKMKFVQKMVIMSSRPIVLAQDIVTDSAVRRLVFDAGEDVENLMTLCEADIT
TKNPSKFKKYHKNFEIVRKKIVEVEERDHVRNFQPPISGEEIMEIFDLKPSREIGILKEAVKEAILEGDIPNEYQAAYDF
VIKRAAKLGLKKVEK
>Mature_495_residues
MHFISTEIRNAFYSKTEFVAIQTNYKTALQNKIFDVISKASQELKVDSYVIGGFVRDLLLNRGSKKDIDVVAVGSGIELA
LKVSDLLPNKPKVQVFKTYGTAMLRFEDTDIEFVGARKESYTRDSRNPIVENGTLQDDQNRRDFTINALALSLNSTNFGD
LLDPFDGLTDLENKTIKTPLDPDITYSDDPLRMLRAIRFATQLNFEIEENSLNAITKNADRIKIISGERIVDELNKILST
PKPSSGFLLLYKTGLLDLILPELTALNQVEEIEGHTHKNNFYHTLEVVDNICPNTDDVWLRWSALLHDIGKAPTKRFTKK
QGWTFHGHEFLGGKMAKKIFERLHMPLNHKMKFVQKMVIMSSRPIVLAQDIVTDSAVRRLVFDAGEDVENLMTLCEADIT
TKNPSKFKKYHKNFEIVRKKIVEVEERDHVRNFQPPISGEEIMEIFDLKPSREIGILKEAVKEAILEGDIPNEYQAAYDF
VIKRAAKLGLKKVEK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=235, Percent_Identity=30.2127659574468, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789436, Length=309, Percent_Identity=31.0679611650485, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081691, Length=245, Percent_Identity=27.7551020408163, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=214, Percent_Identity=31.3084112149533, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=235, Percent_Identity=32.7659574468085, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281360112, Length=251, Percent_Identity=28.2868525896414, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21357337, Length=251, Percent_Identity=28.2868525896414, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 56451; Mature: 56451

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFISTEIRNAFYSKTEFVAIQTNYKTALQNKIFDVISKASQELKVDSYVIGGFVRDLLL
CCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRGSKKDIDVVAVGSGIELALKVSDLLPNKPKVQVFKTYGTAMLRFEDTDIEFVGARKES
CCCCCCCCEEEEECCCCEEEEEEHHHCCCCCCEEEEEECCCEEEEEECCCEEEEECCCHH
YTRDSRNPIVENGTLQDDQNRRDFTINALALSLNSTNFGDLLDPFDGLTDLENKTIKTPL
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHCCCCCCHHCCCCCCCCCC
DPDITYSDDPLRMLRAIRFATQLNFEIEENSLNAITKNADRIKIISGERIVDELNKILST
CCCCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHCCCCEEEEECCHHHHHHHHHHHCC
PKPSSGFLLLYKTGLLDLILPELTALNQVEEIEGHTHKNNFYHTLEVVDNICPNTDDVWL
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHH
RWSALLHDIGKAPTKRFTKKQGWTFHGHEFLGGKMAKKIFERLHMPLNHKMKFVQKMVIM
HHHHHHHHHCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SSRPIVLAQDIVTDSAVRRLVFDAGEDVENLMTLCEADITTKNPSKFKKYHKNFEIVRKK
CCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IVEVEERDHVRNFQPPISGEEIMEIFDLKPSREIGILKEAVKEAILEGDIPNEYQAAYDF
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VIKRAAKLGLKKVEK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFISTEIRNAFYSKTEFVAIQTNYKTALQNKIFDVISKASQELKVDSYVIGGFVRDLLL
CCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRGSKKDIDVVAVGSGIELALKVSDLLPNKPKVQVFKTYGTAMLRFEDTDIEFVGARKES
CCCCCCCCEEEEECCCCEEEEEEHHHCCCCCCEEEEEECCCEEEEEECCCEEEEECCCHH
YTRDSRNPIVENGTLQDDQNRRDFTINALALSLNSTNFGDLLDPFDGLTDLENKTIKTPL
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHCCCCCCHHCCCCCCCCCC
DPDITYSDDPLRMLRAIRFATQLNFEIEENSLNAITKNADRIKIISGERIVDELNKILST
CCCCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHCCCCEEEEECCHHHHHHHHHHHCC
PKPSSGFLLLYKTGLLDLILPELTALNQVEEIEGHTHKNNFYHTLEVVDNICPNTDDVWL
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHH
RWSALLHDIGKAPTKRFTKKQGWTFHGHEFLGGKMAKKIFERLHMPLNHKMKFVQKMVIM
HHHHHHHHHCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SSRPIVLAQDIVTDSAVRRLVFDAGEDVENLMTLCEADITTKNPSKFKKYHKNFEIVRKK
CCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IVEVEERDHVRNFQPPISGEEIMEIFDLKPSREIGILKEAVKEAILEGDIPNEYQAAYDF
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VIKRAAKLGLKKVEK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA