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The map label for this gene is emrB [H]

Identifier: 146302631

GI number: 146302631

Start: 5925668

End: 5927257

Strand: Direct

Name: emrB [H]

Synonym: Fjoh_4904

Alternate gene names: 146302631

Gene position: 5925668-5927257 (Clockwise)

Preceding gene: 146302630

Following gene: 146302633

Centisome position: 97.19

GC content: 37.8

Gene sequence:

>1590_bases
ATGGCAGGAGCAGTACAAGCAGACGACGATTTAGTAGAATACGGTTTCAGACGTGTTATCATTACGATTACAGCAGTGCT
TTGTGCACTGTTGGAAATTGTAGATACTACGATTGTAAACGTAGCGCTGACAGACATGCGCGGAAGTCTTGGTGCTACCT
TGACTGATGTGGCATGGGTTATTACAGCATACGCAATTGCGAATGTTATTGTAATTCCGATGACGAGCTGGCTATCACAG
CAATTTGGAAGACGTAATTATTTTGTGGCCTCCATTATAATATTTACGGTCTGTTCTTTTTTGTGTGGTAATGCCACAAA
TATTTGGGAACTTGTAGCTTTCCGATTCGTACAAGGTATGGGCGGTGGAGCATTACTGGTAACAGCCCAAACGATTATTA
CAGAAAGTTATCCCGTAGCAAAACGTGGAATGGCACAGGCTATTTACGGAATGGGTGTAATTGTAGGTCCAACTTTAGGT
CCGCCTTTGGGAGGATATTTAGTAGATAATTACTCTTGGCCTTATATATTTTACATCAATATTCCGTTGGGAATTATTGC
CACTATTTTGGCTTTAACATTTGTTAGAAGTCCGAAATATGGAGAAAAATTAAAAGCCAATCAGGTTGACTGGTGGGGAA
TTATTCTGTTGAGTACCTTTATTGGTTCTTTACAATTCGTATTAGAACACGGGCAGCAAGACGACTGGTTTAACGATTCA
TTAATCGTAACCTTAAGTGTTGTTACCGTTCTTGGATTGGTTTTATTTATCTGGAGAGAGCTTACTTATAAACATCCAAT
CGTAAACTTAAGTGTTTTAAAAGACGGAAACTTAAGAATTGGAACCGTAATGTGTTTTATTCTTGGTTTCGGTTTATACG
GATCAACATTGATTATTCCAATTTACACGCAGTCAATTTTAGGATGGACTGCAACTGATGCAGGTTTATTATTAATTCCG
GGATCTATTACAACGGCAATTATGATGCCTTTTGTTGGAAATATGATTCAGAAAGGTGTACCGCAAGGATATATGGTTGG
AGTAGGATTTTTAATTTTCTTCTTCTTTACTTTCATGATGTACAGCCGAATGACGCCTGATACTGGAGTTGAACATATGT
ACTGGCCTTTAATTTTGAGAGGAATTGGTCTAGGATTACTTTTCGTTCCTATTACAACACTTTCTCTTTCAACTTTAAAA
GGAAAACAAATTGGTGAAGGAGCAGCATTTACAGGAATGATGCGTCAGTTAGGCGGATCATTTGGTATTGCGATTATTAC
CACTTTCATCACTCGTTTCAGTCAGTCACACAGAGTAGATTTAATTAATAATTTAGATCCGGCGAAATTTGAAGTGCAAC
AGCGAATTGCAGGAATGCAGCACGCGTTCATGGCAAAAGGATATAGCGCAGATGTTGCTTTGAAAAAAGCATATCAGGCT
ATAGAGTATTCTGTAATGAAACAAAGTACTGTAATGGCTTATATGGATATTTTCCTTTATCTGGGAATCATGTTTTTATG
TTGCATACCGATTATTCTCTTTATCAAAAAAGGGAAAAACAAAATTAGTGCAGCCGACGCAATGCATTAA

Upstream 100 bases:

>100_bases
ACCAGTAAAAATTACTATAGACGAATCTAACGATCCTGAAAAAGTAAAACTTTTAAGACCAGGAATGAACGTTGACGTTG
ATGTACATTTAAAATAAAAC

Downstream 100 bases:

>100_bases
TAATAATTTTATAATACGCAGAAACGCCGAATTCATCATTTAGAATTCGGCGTTTATTTTTTTATAGTCGCAGTGTGCAG
TGGCAGTATTCAGTTTATAA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAGAVQADDDLVEYGFRRVIITITAVLCALLEIVDTTIVNVALTDMRGSLGATLTDVAWVITAYAIANVIVIPMTSWLSQ
QFGRRNYFVASIIIFTVCSFLCGNATNIWELVAFRFVQGMGGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLG
PPLGGYLVDNYSWPYIFYINIPLGIIATILALTFVRSPKYGEKLKANQVDWWGIILLSTFIGSLQFVLEHGQQDDWFNDS
LIVTLSVVTVLGLVLFIWRELTYKHPIVNLSVLKDGNLRIGTVMCFILGFGLYGSTLIIPIYTQSILGWTATDAGLLLIP
GSITTAIMMPFVGNMIQKGVPQGYMVGVGFLIFFFFTFMMYSRMTPDTGVEHMYWPLILRGIGLGLLFVPITTLSLSTLK
GKQIGEGAAFTGMMRQLGGSFGIAIITTFITRFSQSHRVDLINNLDPAKFEVQQRIAGMQHAFMAKGYSADVALKKAYQA
IEYSVMKQSTVMAYMDIFLYLGIMFLCCIPIILFIKKGKNKISAADAMH

Sequences:

>Translated_529_residues
MAGAVQADDDLVEYGFRRVIITITAVLCALLEIVDTTIVNVALTDMRGSLGATLTDVAWVITAYAIANVIVIPMTSWLSQ
QFGRRNYFVASIIIFTVCSFLCGNATNIWELVAFRFVQGMGGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLG
PPLGGYLVDNYSWPYIFYINIPLGIIATILALTFVRSPKYGEKLKANQVDWWGIILLSTFIGSLQFVLEHGQQDDWFNDS
LIVTLSVVTVLGLVLFIWRELTYKHPIVNLSVLKDGNLRIGTVMCFILGFGLYGSTLIIPIYTQSILGWTATDAGLLLIP
GSITTAIMMPFVGNMIQKGVPQGYMVGVGFLIFFFFTFMMYSRMTPDTGVEHMYWPLILRGIGLGLLFVPITTLSLSTLK
GKQIGEGAAFTGMMRQLGGSFGIAIITTFITRFSQSHRVDLINNLDPAKFEVQQRIAGMQHAFMAKGYSADVALKKAYQA
IEYSVMKQSTVMAYMDIFLYLGIMFLCCIPIILFIKKGKNKISAADAMH
>Mature_528_residues
AGAVQADDDLVEYGFRRVIITITAVLCALLEIVDTTIVNVALTDMRGSLGATLTDVAWVITAYAIANVIVIPMTSWLSQQ
FGRRNYFVASIIIFTVCSFLCGNATNIWELVAFRFVQGMGGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLGP
PLGGYLVDNYSWPYIFYINIPLGIIATILALTFVRSPKYGEKLKANQVDWWGIILLSTFIGSLQFVLEHGQQDDWFNDSL
IVTLSVVTVLGLVLFIWRELTYKHPIVNLSVLKDGNLRIGTVMCFILGFGLYGSTLIIPIYTQSILGWTATDAGLLLIPG
SITTAIMMPFVGNMIQKGVPQGYMVGVGFLIFFFFTFMMYSRMTPDTGVEHMYWPLILRGIGLGLLFVPITTLSLSTLKG
KQIGEGAAFTGMMRQLGGSFGIAIITTFITRFSQSHRVDLINNLDPAKFEVQQRIAGMQHAFMAKGYSADVALKKAYQAI
EYSVMKQSTVMAYMDIFLYLGIMFLCCIPIILFIKKGKNKISAADAMH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=504, Percent_Identity=32.1428571428571, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=31.1377245508982, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=28.780487804878, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=26.1083743842365, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788392, Length=415, Percent_Identity=28.1927710843373, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319770, Length=411, Percent_Identity=25.0608272506083, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=277, Percent_Identity=25.2707581227437, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=309, Percent_Identity=22.9773462783171, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58195; Mature: 58064

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAVQADDDLVEYGFRRVIITITAVLCALLEIVDTTIVNVALTDMRGSLGATLTDVAWV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ITAYAIANVIVIPMTSWLSQQFGRRNYFVASIIIFTVCSFLCGNATNIWELVAFRFVQGM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
GGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLGPPLGGYLVDNYSWPYIFYIN
CCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCEEECCCCCCEEEEEE
IPLGIIATILALTFVRSPKYGEKLKANQVDWWGIILLSTFIGSLQFVLEHGQQDDWFNDS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LIVTLSVVTVLGLVLFIWRELTYKHPIVNLSVLKDGNLRIGTVMCFILGFGLYGSTLIIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEE
IYTQSILGWTATDAGLLLIPGSITTAIMMPFVGNMIQKGVPQGYMVGVGFLIFFFFTFMM
EHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YSRMTPDTGVEHMYWPLILRGIGLGLLFVPITTLSLSTLKGKQIGEGAAFTGMMRQLGGS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
FGIAIITTFITRFSQSHRVDLINNLDPAKFEVQQRIAGMQHAFMAKGYSADVALKKAYQA
CHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IEYSVMKQSTVMAYMDIFLYLGIMFLCCIPIILFIKKGKNKISAADAMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
AGAVQADDDLVEYGFRRVIITITAVLCALLEIVDTTIVNVALTDMRGSLGATLTDVAWV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ITAYAIANVIVIPMTSWLSQQFGRRNYFVASIIIFTVCSFLCGNATNIWELVAFRFVQGM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
GGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLGPPLGGYLVDNYSWPYIFYIN
CCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCEEECCCCCCEEEEEE
IPLGIIATILALTFVRSPKYGEKLKANQVDWWGIILLSTFIGSLQFVLEHGQQDDWFNDS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LIVTLSVVTVLGLVLFIWRELTYKHPIVNLSVLKDGNLRIGTVMCFILGFGLYGSTLIIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEE
IYTQSILGWTATDAGLLLIPGSITTAIMMPFVGNMIQKGVPQGYMVGVGFLIFFFFTFMM
EHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YSRMTPDTGVEHMYWPLILRGIGLGLLFVPITTLSLSTLKGKQIGEGAAFTGMMRQLGGS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
FGIAIITTFITRFSQSHRVDLINNLDPAKFEVQQRIAGMQHAFMAKGYSADVALKKAYQA
CHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IEYSVMKQSTVMAYMDIFLYLGIMFLCCIPIILFIKKGKNKISAADAMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]