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The map label for this gene is yfiK [H]

Identifier: 146302462

GI number: 146302462

Start: 5725551

End: 5726180

Strand: Direct

Name: yfiK [H]

Synonym: Fjoh_4735

Alternate gene names: 146302462

Gene position: 5725551-5726180 (Clockwise)

Preceding gene: 146302455

Following gene: 146302463

Centisome position: 93.91

GC content: 38.41

Gene sequence:

>630_bases
ATGAAAATAAGAGTTGCCATAGTAGAAGATGATAAACATTACAGTCAGGCCCTGAAGAGTATCATCGATTTTCAGGAAGA
CATGGAATGCGCAGCTCAGTTTTTTAATGGTAAAAGCGCATTGCAAAAATTGGAAGACCTGGAACCAGACGTGGTTTTAA
TGGATATTCAGCTTCAGGATGCATCAGGAATTGATATCGTTTCTAAATTGACAGAAGCAATGCCGGGAACGAGTTTTATA
ATGTGCACCAGTTTCGAAAACGACGAAAAAATCTTTTCGGCCCTGCGTGCAGGCGCGAGCGGTTATCTTGTAAAAGGTGA
TCCTCTCGATAAAATTATTCAGGCTATTCTCGAAGCTCATAAAGGAGGCGCACCCATGAGTTTTACAATTGCAAAGCGGG
TTTTACAGCATTTTCAGGAATCGAAAGTACAGGTACAAAGTTTGGCCTTACTTACCGTAACCGAAAAAGAAGTTCTTGAA
CTGCTGGCACAAGGACTGCTCTACAAAGAAATTGCCGATAAAAAAAATGTCACCATCGACACTATAAAAAAACACATTGG
CAATATCTACAGAAAATTACAAGTAAGTAATAAAATTGAAGCTATTAATAAGTTTAACACCAAAAACTAA

Upstream 100 bases:

>100_bases
GTAAGTTTATCCACGTTAAGCTTAATTCTGAAATATCACCCAAAAGGGTAATTGTGGTCAAAATACTTATTACCCAACTT
TGTAATGAAAATGTAACAAC

Downstream 100 bases:

>100_bases
CAAATCATGGAACTAGAAAAATTAAAACAAGAAAAAATCGATGTAATTGATTTTGCAAAAAAAATTGATAGAGAAATCGA
AAATCTTTTTGTCGAGAACT

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKIRVAIVEDDKHYSQALKSIIDFQEDMECAAQFFNGKSALQKLEDLEPDVVLMDIQLQDASGIDIVSKLTEAMPGTSFI
MCTSFENDEKIFSALRAGASGYLVKGDPLDKIIQAILEAHKGGAPMSFTIAKRVLQHFQESKVQVQSLALLTVTEKEVLE
LLAQGLLYKEIADKKNVTIDTIKKHIGNIYRKLQVSNKIEAINKFNTKN

Sequences:

>Translated_209_residues
MKIRVAIVEDDKHYSQALKSIIDFQEDMECAAQFFNGKSALQKLEDLEPDVVLMDIQLQDASGIDIVSKLTEAMPGTSFI
MCTSFENDEKIFSALRAGASGYLVKGDPLDKIIQAILEAHKGGAPMSFTIAKRVLQHFQESKVQVQSLALLTVTEKEVLE
LLAQGLLYKEIADKKNVTIDTIKKHIGNIYRKLQVSNKIEAINKFNTKN
>Mature_209_residues
MKIRVAIVEDDKHYSQALKSIIDFQEDMECAAQFFNGKSALQKLEDLEPDVVLMDIQLQDASGIDIVSKLTEAMPGTSFI
MCTSFENDEKIFSALRAGASGYLVKGDPLDKIIQAILEAHKGGAPMSFTIAKRVLQHFQESKVQVQSLALLTVTEKEVLE
LLAQGLLYKEIADKKNVTIDTIKKHIGNIYRKLQVSNKIEAINKFNTKN

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=29.5566502463054, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787473, Length=198, Percent_Identity=24.2424242424242, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=32.7731092436975, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788222, Length=199, Percent_Identity=22.1105527638191, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23317; Mature: 23317

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRVAIVEDDKHYSQALKSIIDFQEDMECAAQFFNGKSALQKLEDLEPDVVLMDIQLQD
CEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECC
ASGIDIVSKLTEAMPGTSFIMCTSFENDEKIFSALRAGASGYLVKGDPLDKIIQAILEAH
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
KGGAPMSFTIAKRVLQHFQESKVQVQSLALLTVTEKEVLELLAQGLLYKEIADKKNVTID
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCCCCHHH
TIKKHIGNIYRKLQVSNKIEAINKFNTKN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIRVAIVEDDKHYSQALKSIIDFQEDMECAAQFFNGKSALQKLEDLEPDVVLMDIQLQD
CEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECC
ASGIDIVSKLTEAMPGTSFIMCTSFENDEKIFSALRAGASGYLVKGDPLDKIIQAILEAH
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
KGGAPMSFTIAKRVLQHFQESKVQVQSLALLTVTEKEVLELLAQGLLYKEIADKKNVTID
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCCCCHHH
TIKKHIGNIYRKLQVSNKIEAINKFNTKN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]