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The map label for this gene is rhgT [H]

Identifier: 146301982

GI number: 146301982

Start: 5124512

End: 5125168

Strand: Reverse

Name: rhgT [H]

Synonym: Fjoh_4246

Alternate gene names: 146301982

Gene position: 5125168-5124512 (Counterclockwise)

Preceding gene: 146301983

Following gene: 146301981

Centisome position: 84.06

GC content: 33.79

Gene sequence:

>657_bases
ATGGCCAATAAGAAAGATCCGGATCGAAATCCAGAACACGGATGGGCTCAAGTATTGCAGTCTTTCTTTAATGATGATAT
TATAGTAGTAAACAAAGCCGTAAATGGCAGAAGTACCAAAAGCTTTATAAATGAAAAGCGCTGGGACTCTATTTATAAAA
AACTGAAAAAAGGAGATTACGTATTTATAGAATTTGGGCATAACGATGAAAAAATAGAAGACTCAACCCGTTATACCAAT
CCACATACTACATATAGGTATAATTTGATTCGTTTTGTAAAAGAAAGTCGTGAAAAAGGAGCCATTCCGGTTTTGCTTAC
ATCTATTGCCAGACGTAATTTTAATGAAAAAGGAGTTTTAGTTCCTACTCATGGCGATTATCCGCTTGAAACACGTTTAA
CTGCACAAGAATATAAAGTACCTTTTATAGATTTAGAATACTATACAGAATTGTTAGAACAATCTTATGGTCCGGAAAAA
TCAAAAAAACTGCATCTTCATTTTAAAGCAGCAGAGAATCCATATTATGATAAAGACAAAGCAGATGATACACATCTTTC
TTTAGAAGGGGCAAAAGCAATAGCACAAATTGTAATCAATAGGATTAAAGCGGAAGAAGATCCGTCTCTTTCAAGGCTTA
AAAAGGGAATCAAATAG

Upstream 100 bases:

>100_bases
ATAAAAAGAATAAAATGAAAAAATCATCTGCTTAATATTATTAATCTCCACAACCTGTTTCGCACAAAAAACAACCTTAT
ATACTATAGGTGACTCCACA

Downstream 100 bases:

>100_bases
TATCAAACGCTATTACTACTTAAATGTAAAGAGGATTAATACTTCTCGATGCCGATCTATTATATAATATAGACTATAAT
TAAAGAGAATATATAGATGA

Product: GDSL family lipase

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MANKKDPDRNPEHGWAQVLQSFFNDDIIVVNKAVNGRSTKSFINEKRWDSIYKKLKKGDYVFIEFGHNDEKIEDSTRYTN
PHTTYRYNLIRFVKESREKGAIPVLLTSIARRNFNEKGVLVPTHGDYPLETRLTAQEYKVPFIDLEYYTELLEQSYGPEK
SKKLHLHFKAAENPYYDKDKADDTHLSLEGAKAIAQIVINRIKAEEDPSLSRLKKGIK

Sequences:

>Translated_218_residues
MANKKDPDRNPEHGWAQVLQSFFNDDIIVVNKAVNGRSTKSFINEKRWDSIYKKLKKGDYVFIEFGHNDEKIEDSTRYTN
PHTTYRYNLIRFVKESREKGAIPVLLTSIARRNFNEKGVLVPTHGDYPLETRLTAQEYKVPFIDLEYYTELLEQSYGPEK
SKKLHLHFKAAENPYYDKDKADDTHLSLEGAKAIAQIVINRIKAEEDPSLSRLKKGIK
>Mature_217_residues
ANKKDPDRNPEHGWAQVLQSFFNDDIIVVNKAVNGRSTKSFINEKRWDSIYKKLKKGDYVFIEFGHNDEKIEDSTRYTNP
HTTYRYNLIRFVKESREKGAIPVLLTSIARRNFNEKGVLVPTHGDYPLETRLTAQEYKVPFIDLEYYTELLEQSYGPEKS
KKLHLHFKAAENPYYDKDKADDTHLSLEGAKAIAQIVINRIKAEEDPSLSRLKKGIK

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 25352; Mature: 25221

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKDPDRNPEHGWAQVLQSFFNDDIIVVNKAVNGRSTKSFINEKRWDSIYKKLKKGDY
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCE
VFIEFGHNDEKIEDSTRYTNPHTTYRYNLIRFVKESREKGAIPVLLTSIARRNFNEKGVL
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEE
VPTHGDYPLETRLTAQEYKVPFIDLEYYTELLEQSYGPEKSKKLHLHFKAAENPYYDKDK
EECCCCCCCCCCCCHHHHCCCEEEHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCC
ADDTHLSLEGAKAIAQIVINRIKAEEDPSLSRLKKGIK
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
ANKKDPDRNPEHGWAQVLQSFFNDDIIVVNKAVNGRSTKSFINEKRWDSIYKKLKKGDY
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCE
VFIEFGHNDEKIEDSTRYTNPHTTYRYNLIRFVKESREKGAIPVLLTSIARRNFNEKGVL
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEE
VPTHGDYPLETRLTAQEYKVPFIDLEYYTELLEQSYGPEKSKKLHLHFKAAENPYYDKDK
EECCCCCCCCCCCCHHHHCCCEEEHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCC
ADDTHLSLEGAKAIAQIVINRIKAEEDPSLSRLKKGIK
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]