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The map label for this gene is hypB [H]

Identifier: 146301643

GI number: 146301643

Start: 4650490

End: 4651344

Strand: Reverse

Name: hypB [H]

Synonym: Fjoh_3904

Alternate gene names: 146301643

Gene position: 4651344-4650490 (Counterclockwise)

Preceding gene: 146301644

Following gene: 146301642

Centisome position: 76.29

GC content: 36.02

Gene sequence:

>855_bases
ATGTGTACCACTTGCGGATGCAGTTCTGATGAAAACGAAATACGATTTACCAAAATAGGAGAGAAACAATTCCATTCTCA
TTCCGGTCACTATCACCACCACGATCACGATCATGACCACCACCATGAGCATCATCACGACCATGATCATGACAACGACG
ATCACAATCATCATCATGACCATTTTCACAATCATTCTCATGAAATAAGTGTGATACAGTTAGAAAAGGATATTTTGTAT
AAAAACCAGCTTACTGCTGAGAGAAACAGAGGATTTTTTGAAGCCCTGAATATTTTTTCTATCAATCTGGTAAGTTCTCC
GGGCTCCGGAAAAACCTCTTTATTAGAAAGAACCATTTCAGATTTAAAAGACAAAATTGCTTTTTCTGTAATCGAAGGTG
ACCAGCAGACTACAAATGATGCCGACAGAATCCATAAATTAAATGTTCCTGTAATTCAGATTAATACAGGAAAAGGCTGC
CATTTAGACAGCGAAATGATATCAAAAGCCGTAAAAGAATTAAAACCCGTTCAGGATTCTGTTTTAATGATCGAAAATGT
TGGAAATCTGGTTTGTCCGGCTATGTTTGATTTGGGAGAATTAAAACGAATCGTAATTATATCAGTTACCGAAGGTGAGG
ATAAACCGCTTAAATATCCGGATATGTTCTCAGGATCGCAAATCTGTATTATTAATAAAATTGATCTGCTTCCGTATTTG
AAATTTGATTTGGAGAAACTGAAAGAATATGCAAAACAGGTAAATCCGCATTTGACTTTCTTTGAAGTTTCTGCAGCATC
AGGAGAAGGTTTAGCAGCCTGGTACGATTATTTAACAAAATCTATAAAACAATAA

Upstream 100 bases:

>100_bases
AAAGTTTTGACAGCTGCCCAAATTGTAAGAGTCCGTTTAAGGAAATTATTGCCGGCAGAGAATTGAAAATAAAGAAAATA
ATCATAAAATAAACTGAGTT

Downstream 100 bases:

>100_bases
GTATGTGTTTAGCAGTTCCCGGTCGACTTGAAAAAATCACAGATGAGCTTGATGAAACGTTTAGAATTGGAAATGTTTCT
TTTGACGGTATTATCAAAGA

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MCTTCGCSSDENEIRFTKIGEKQFHSHSGHYHHHDHDHDHHHEHHHDHDHDNDDHNHHHDHFHNHSHEISVIQLEKDILY
KNQLTAERNRGFFEALNIFSINLVSSPGSGKTSLLERTISDLKDKIAFSVIEGDQQTTNDADRIHKLNVPVIQINTGKGC
HLDSEMISKAVKELKPVQDSVLMIENVGNLVCPAMFDLGELKRIVIISVTEGEDKPLKYPDMFSGSQICIINKIDLLPYL
KFDLEKLKEYAKQVNPHLTFFEVSAASGEGLAAWYDYLTKSIKQ

Sequences:

>Translated_284_residues
MCTTCGCSSDENEIRFTKIGEKQFHSHSGHYHHHDHDHDHHHEHHHDHDHDNDDHNHHHDHFHNHSHEISVIQLEKDILY
KNQLTAERNRGFFEALNIFSINLVSSPGSGKTSLLERTISDLKDKIAFSVIEGDQQTTNDADRIHKLNVPVIQINTGKGC
HLDSEMISKAVKELKPVQDSVLMIENVGNLVCPAMFDLGELKRIVIISVTEGEDKPLKYPDMFSGSQICIINKIDLLPYL
KFDLEKLKEYAKQVNPHLTFFEVSAASGEGLAAWYDYLTKSIKQ
>Mature_284_residues
MCTTCGCSSDENEIRFTKIGEKQFHSHSGHYHHHDHDHDHHHEHHHDHDHDNDDHNHHHDHFHNHSHEISVIQLEKDILY
KNQLTAERNRGFFEALNIFSINLVSSPGSGKTSLLERTISDLKDKIAFSVIEGDQQTTNDADRIHKLNVPVIQINTGKGC
HLDSEMISKAVKELKPVQDSVLMIENVGNLVCPAMFDLGELKRIVIISVTEGEDKPLKYPDMFSGSQICIINKIDLLPYL
KFDLEKLKEYAKQVNPHLTFFEVSAASGEGLAAWYDYLTKSIKQ

Specific function: May work in the mobilization of nickel into hydrogenase enzyme. Binds 9 nickel ions per molecule [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=297, Percent_Identity=43.7710437710438, Blast_Score=250, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 32435; Mature: 32435

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTTCGCSSDENEIRFTKIGEKQFHSHSGHYHHHDHDHDHHHEHHHDHDHDNDDHNHHHD
CCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
HFHNHSHEISVIQLEKDILYKNQLTAERNRGFFEALNIFSINLVSSPGSGKTSLLERTIS
HHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHH
DLKDKIAFSVIEGDQQTTNDADRIHKLNVPVIQINTGKGCHLDSEMISKAVKELKPVQDS
HHHHHHEEEEECCCCCCCCHHHHEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHH
VLMIENVGNLVCPAMFDLGELKRIVIISVTEGEDKPLKYPDMFSGSQICIINKIDLLPYL
EEEEHHCCCHHHHHHHCHHCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHH
KFDLEKLKEYAKQVNPHLTFFEVSAASGEGLAAWYDYLTKSIKQ
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MCTTCGCSSDENEIRFTKIGEKQFHSHSGHYHHHDHDHDHHHEHHHDHDHDNDDHNHHHD
CCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
HFHNHSHEISVIQLEKDILYKNQLTAERNRGFFEALNIFSINLVSSPGSGKTSLLERTIS
HHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHH
DLKDKIAFSVIEGDQQTTNDADRIHKLNVPVIQINTGKGCHLDSEMISKAVKELKPVQDS
HHHHHHEEEEECCCCCCCCHHHHEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHH
VLMIENVGNLVCPAMFDLGELKRIVIISVTEGEDKPLKYPDMFSGSQICIINKIDLLPYL
EEEEHHCCCHHHHHHHCHHCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHH
KFDLEKLKEYAKQVNPHLTFFEVSAASGEGLAAWYDYLTKSIKQ
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8305450; 12597275 [H]