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The map label for this gene is insB1 [H]

Identifier: 146301435

GI number: 146301435

Start: 4386279

End: 4386968

Strand: Reverse

Name: insB1 [H]

Synonym: Fjoh_3693

Alternate gene names: 146301435

Gene position: 4386968-4386279 (Counterclockwise)

Preceding gene: 146301436

Following gene: 146301433

Centisome position: 71.95

GC content: 31.45

Gene sequence:

>690_bases
ATGCGACATAGTGTTCCAAAGTTAGTGATGGTAATAAATTGTCCAAAATGTAAAGAGAAAAAGGTTATTAAAAATGGGAC
AACAAAAAACAATAAACAGCAGTATTATTGCAAAATGTGCTTTTACCGTTTTATACAAAATTATACAAATCAAGCTTACA
AATTAGATATTAATCAAAAGAATGTTCAATTAACCAAAGAGGGATTAGGTATAAGAAGCACTGCAAGAATATTGGAAATA
TCAGCCACAACATTATTGAAAAGAATTGTGTCAATTGGCAGAAAAATTAATCAGCCGATTATAAGCAAATGTAAAACTTA
TGAAGTTGATGAAATGTGTACTTACATAGGAAGTAAGCAAAATTTTATCTGGCTGGTTTATGCATTGGACAAGAATTCTA
AAACTGTTGTCAGTTTTAATGTTGCTAAACGAACCAACAAAACTCTAAGCCGTGTTCTGGATACTTTAAAACTATCTGAA
GCAAAAAAGATATTTACTGGCAGACTTAAAAACTACAGGTATCTGCTTGATGAGAAAATGCATTCAGTAAAACGTTTCGG
GACAAACCATATCGAAAGAAAGAATCTGACACTCAGAACTCATCTGAAAAGACTCAACAGGCGGACAATCTGCTCCAGTA
AAAGTTTATTGATTTTTACTGCTGTTTTGAAGATTTATTTTTGGATTTGA

Upstream 100 bases:

>100_bases
TGAAATTGAACAAATTTTAGAAATAAATAATTTAAAGATGAGTAATAATGCTCATCTTTTTTGTTTTAATATTGCCTTAA
ATAAGCTAAAATGAGTAAAA

Downstream 100 bases:

>100_bases
GCAATAAAAGAGTAAGTTTATCCTTTAAAATCATGTTCTTAAAAATTTAGCATTTAACAGGTAAATACTTGTAAAAACAG
TCACAGACCCTTGTAAGTAA

Product: IS1 transposase

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRHSVPKLVMVINCPKCKEKKVIKNGTTKNNKQQYYCKMCFYRFIQNYTNQAYKLDINQKNVQLTKEGLGIRSTARILEI
SATTLLKRIVSIGRKINQPIISKCKTYEVDEMCTYIGSKQNFIWLVYALDKNSKTVVSFNVAKRTNKTLSRVLDTLKLSE
AKKIFTGRLKNYRYLLDEKMHSVKRFGTNHIERKNLTLRTHLKRLNRRTICSSKSLLIFTAVLKIYFWI

Sequences:

>Translated_229_residues
MRHSVPKLVMVINCPKCKEKKVIKNGTTKNNKQQYYCKMCFYRFIQNYTNQAYKLDINQKNVQLTKEGLGIRSTARILEI
SATTLLKRIVSIGRKINQPIISKCKTYEVDEMCTYIGSKQNFIWLVYALDKNSKTVVSFNVAKRTNKTLSRVLDTLKLSE
AKKIFTGRLKNYRYLLDEKMHSVKRFGTNHIERKNLTLRTHLKRLNRRTICSSKSLLIFTAVLKIYFWI
>Mature_229_residues
MRHSVPKLVMVINCPKCKEKKVIKNGTTKNNKQQYYCKMCFYRFIQNYTNQAYKLDINQKNVQLTKEGLGIRSTARILEI
SATTLLKRIVSIGRKINQPIISKCKTYEVDEMCTYIGSKQNFIWLVYALDKNSKTVVSFNVAKRTNKTLSRVLDTLKLSE
AKKIFTGRLKNYRYLLDEKMHSVKRFGTNHIERKNLTLRTHLKRLNRRTICSSKSLLIFTAVLKIYFWI

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1787223, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789853, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788203, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786203, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786470, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786459, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 26917; Mature: 26917

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSVPKLVMVINCPKCKEKKVIKNGTTKNNKQQYYCKMCFYRFIQNYTNQAYKLDINQK
CCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
NVQLTKEGLGIRSTARILEISATTLLKRIVSIGRKINQPIISKCKTYEVDEMCTYIGSKQ
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCC
NFIWLVYALDKNSKTVVSFNVAKRTNKTLSRVLDTLKLSEAKKIFTGRLKNYRYLLDEKM
CEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSVKRFGTNHIERKNLTLRTHLKRLNRRTICSSKSLLIFTAVLKIYFWI
HHHHHHCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHSVPKLVMVINCPKCKEKKVIKNGTTKNNKQQYYCKMCFYRFIQNYTNQAYKLDINQK
CCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
NVQLTKEGLGIRSTARILEISATTLLKRIVSIGRKINQPIISKCKTYEVDEMCTYIGSKQ
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCC
NFIWLVYALDKNSKTVVSFNVAKRTNKTLSRVLDTLKLSEAKKIFTGRLKNYRYLLDEKM
CEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSVKRFGTNHIERKNLTLRTHLKRLNRRTICSSKSLLIFTAVLKIYFWI
HHHHHHCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]