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The map label for this gene is 146301151

Identifier: 146301151

GI number: 146301151

Start: 4072829

End: 4073422

Strand: Reverse

Name: 146301151

Synonym: Fjoh_3409

Alternate gene names: NA

Gene position: 4073422-4072829 (Counterclockwise)

Preceding gene: 146301152

Following gene: 146301150

Centisome position: 66.81

GC content: 36.36

Gene sequence:

>594_bases
ATGGGCGTATTAAGATTACAATTGCCAACAGACCCAAGATGGGTAAATATCGTTGAGAAAAACATCGAAGAGATTCTGAC
AGATCACGCTTGGTGCGAGCAGAAAGCAGCAACAAACGCAATTACAATTATTACCAATAATTCTGAACATCAGGATTTAG
TAAAAGATTTATTGGCTTTAGCCAAAGAAGAAATTGATCATTTTGAACAGGTTCACAACATTATCATCAAAAGAGGATTA
AAATTAGGACGTGAGCGTAAAGATGATTACGTAAATGAACTATACCAATACATGAAAAGAAGCGGAGACGGAAGCCGTGT
TTCTGGTCTTGTTGAAAGATTATTATTCTCAGCAATGATTGAAGCAAGAAGCTGTGAACGTTTTAAAGTGCTTTCAGAAA
ATATTAAAGACGAAGAATTAGCTGTTTTCTATAGAGAATTAATGGAAAGTGAAGCAGGACATTATACCACTTTCATCACA
TACGCACGTAAATACGGAACCGGAATAGACGTTGAAAAACGCTGGAGAGAATGGTTAGCATTCGAAGAATCAATTATTAC
CAATTACGGAAAAGGAGAAACGATTCACGGGTAG

Upstream 100 bases:

>100_bases
AATGTGAATTTCTATGTTTAGTTTTTGATCTTCGATTTTTCTTTTTAGGTTTTCTAATGACTTTATAACTTTTGACTTTC
AAACCTTAAGACTTAAAAAA

Downstream 100 bases:

>100_bases
TTTTTTTAGTTTTCAGTCTCAGTTTTCAGTCTCAGTTTTCAGTCTCAGTTTTCAGTCTCAGTTTTCAGTCTCACTTTTCA
GTTTTCACGTTTGTCAGGCT

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: TRNA-(MS[2]IO[6]A)-Hydroxylase; TRNA-(Ms[2]Io[6]A)-Hydroxylase; TRNA-(Ms(2)Io(6)A)-Hydroxylase; TRNA-(MS[2]IO[6]A)-Hydroxylase Subfamily; tRNA Hydroxylase; TRNA-(MS(2)IO(6)A)-Hydroxylase-Like; TRNA-(MS[2]IO[6]A)-Hydroxylase-Like Protein; TRNA-(MS(2)IO(6)A)-Hydroxylase; Probably TRNA-(MS[2]IO[6]A)-Hydroxylase; Hydroxylase; TRNA-(MS 2)O6-A-Hydroxylase; tRNA Hydroxylase-Like Protein

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MGVLRLQLPTDPRWVNIVEKNIEEILTDHAWCEQKAATNAITIITNNSEHQDLVKDLLALAKEEIDHFEQVHNIIIKRGL
KLGRERKDDYVNELYQYMKRSGDGSRVSGLVERLLFSAMIEARSCERFKVLSENIKDEELAVFYRELMESEAGHYTTFIT
YARKYGTGIDVEKRWREWLAFEESIITNYGKGETIHG

Sequences:

>Translated_197_residues
MGVLRLQLPTDPRWVNIVEKNIEEILTDHAWCEQKAATNAITIITNNSEHQDLVKDLLALAKEEIDHFEQVHNIIIKRGL
KLGRERKDDYVNELYQYMKRSGDGSRVSGLVERLLFSAMIEARSCERFKVLSENIKDEELAVFYRELMESEAGHYTTFIT
YARKYGTGIDVEKRWREWLAFEESIITNYGKGETIHG
>Mature_196_residues
GVLRLQLPTDPRWVNIVEKNIEEILTDHAWCEQKAATNAITIITNNSEHQDLVKDLLALAKEEIDHFEQVHNIIIKRGLK
LGRERKDDYVNELYQYMKRSGDGSRVSGLVERLLFSAMIEARSCERFKVLSENIKDEELAVFYRELMESEAGHYTTFITY
ARKYGTGIDVEKRWREWLAFEESIITNYGKGETIHG

Specific function: Unknown

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22954; Mature: 22823

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLRLQLPTDPRWVNIVEKNIEEILTDHAWCEQKAATNAITIITNNSEHQDLVKDLLAL
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
AKEEIDHFEQVHNIIIKRGLKLGRERKDDYVNELYQYMKRSGDGSRVSGLVERLLFSAMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EARSCERFKVLSENIKDEELAVFYRELMESEAGHYTTFITYARKYGTGIDVEKRWREWLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
FEESIITNYGKGETIHG
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GVLRLQLPTDPRWVNIVEKNIEEILTDHAWCEQKAATNAITIITNNSEHQDLVKDLLAL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
AKEEIDHFEQVHNIIIKRGLKLGRERKDDYVNELYQYMKRSGDGSRVSGLVERLLFSAMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EARSCERFKVLSENIKDEELAVFYRELMESEAGHYTTFITYARKYGTGIDVEKRWREWLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
FEESIITNYGKGETIHG
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA