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The map label for this gene is glyA

Identifier: 146301152

GI number: 146301152

Start: 4073571

End: 4074845

Strand: Reverse

Name: glyA

Synonym: Fjoh_3410

Alternate gene names: 146301152

Gene position: 4074845-4073571 (Counterclockwise)

Preceding gene: 146301154

Following gene: 146301151

Centisome position: 66.84

GC content: 38.04

Gene sequence:

>1275_bases
ATGCAACGCGACGAACAAATTTTTGATCTTATCCAAGAGGAAAAAGATAGACAAATTCACGGATTAGAACTTATCGCTTC
AGAAAATTTTGTAAGTGATGAGGTAATGGAAGCAGCTGGGTCTGTTTTAACTAACAAATATGCTGAGGGTTATCCTGGCA
AAAGATACTACGGCGGTTGTGAAGTAGTTGACGTTATCGAGCAGATTGCTATCGACAGAGCAAAAGAATTATTTGGAGCT
GAATATGCAAACGTACAGCCTCACTCAGGTTCTCAGGCAAACACTGCTGTTTACCACGCTTGTTTAAATCCTGGTGATAC
TATTTTAGGTTTTGACTTATCTCATGGTGGTCACTTAACGCACGGTTCGCCTGTAAACTTCTCAGGACGTTTATACCGTC
CGGTTTTCTACGGTGTAGATGCTGAAACAGGACGTTTAGATTATGATAAAATTCAGGAAATTGCAACTAAAGAACAGCCA
AAATTAATCATCGCAGGAGCTTCGGCTTATTCTCGTGATATGGATTTTGCTCGTTTCAGACAAATTGCAGACAGCGTAGG
AGCGATCTTATTTGCTGATATTTCTCACCCGGCAGGGCTTATTGCAAAAGGATTATTAAGCGATCCAATTCCACATTGTC
ATATCGTTTCTACAACGACTCACAAAACATTAAGAGGACCACGTGGAGGTCTAATCTTAATGGGGAAAGATTTCCCAAAT
CCACAAGGGTTAACAACTCCAAAAGGAGAAATCAGAATGATGTCTTCATTATTAGACTTAGCTGTTTTCCCAGGAAACCA
AGGTGGACCTTTAATGCATATTATCGCTGCTAAAGCGGTTGCTTTTGGTGAAGCTCTTAAAGATGAGTTCTTTACTTATG
CAATGCAGTTGCAAAAAAATGCAAACGCAATGGCAGATGCTTTCGTAAAAAGAGGATACAACATTATCTCTGGCGGAACT
GATAACCACATGATGCTTATCGATTTAAGAAATAAAAATATTTCTGGTAAAGAAGCTGAAAATGCATTAGTAAAAGCAGA
AATTACAGTAAACAAAAACATGGTTCCTTTTGATGATAAATCACCATTTATCACTTCTGGAATTCGTGTTGGAACAGCTG
CAATCACAACTCGTGGTTTAGTTGAAAAAGATATGGAAACTATCGTAGCACTTATCGATAAAGTATTAACAAATCATACA
AATGAAGACGTTATCGAAGAAGTTGCTGAAGAAGTAAACGAATTAATGAGCGAAAGACCAATTTTTGCGTATTAA

Upstream 100 bases:

>100_bases
TAATTATTTGTTGAAAAATTATATGTCAAATATATCATAATCTGCCAGTATCACAAACGTTTTTTTGTATTTTTGCAGGA
AATTAACGAAAAACGAAAAA

Downstream 100 bases:

>100_bases
AAATTAGTCGTAAAGTCTTAAAGTCGAAGGTCAAAAGTCTAAAAATGGAATGTGAATTTCTATGTTTAGTTTTTGATCTT
CGATTTTTCTTTTTAGGTTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MQRDEQIFDLIQEEKDRQIHGLELIASENFVSDEVMEAAGSVLTNKYAEGYPGKRYYGGCEVVDVIEQIAIDRAKELFGA
EYANVQPHSGSQANTAVYHACLNPGDTILGFDLSHGGHLTHGSPVNFSGRLYRPVFYGVDAETGRLDYDKIQEIATKEQP
KLIIAGASAYSRDMDFARFRQIADSVGAILFADISHPAGLIAKGLLSDPIPHCHIVSTTTHKTLRGPRGGLILMGKDFPN
PQGLTTPKGEIRMMSSLLDLAVFPGNQGGPLMHIIAAKAVAFGEALKDEFFTYAMQLQKNANAMADAFVKRGYNIISGGT
DNHMMLIDLRNKNISGKEAENALVKAEITVNKNMVPFDDKSPFITSGIRVGTAAITTRGLVEKDMETIVALIDKVLTNHT
NEDVIEEVAEEVNELMSERPIFAY

Sequences:

>Translated_424_residues
MQRDEQIFDLIQEEKDRQIHGLELIASENFVSDEVMEAAGSVLTNKYAEGYPGKRYYGGCEVVDVIEQIAIDRAKELFGA
EYANVQPHSGSQANTAVYHACLNPGDTILGFDLSHGGHLTHGSPVNFSGRLYRPVFYGVDAETGRLDYDKIQEIATKEQP
KLIIAGASAYSRDMDFARFRQIADSVGAILFADISHPAGLIAKGLLSDPIPHCHIVSTTTHKTLRGPRGGLILMGKDFPN
PQGLTTPKGEIRMMSSLLDLAVFPGNQGGPLMHIIAAKAVAFGEALKDEFFTYAMQLQKNANAMADAFVKRGYNIISGGT
DNHMMLIDLRNKNISGKEAENALVKAEITVNKNMVPFDDKSPFITSGIRVGTAAITTRGLVEKDMETIVALIDKVLTNHT
NEDVIEEVAEEVNELMSERPIFAY
>Mature_424_residues
MQRDEQIFDLIQEEKDRQIHGLELIASENFVSDEVMEAAGSVLTNKYAEGYPGKRYYGGCEVVDVIEQIAIDRAKELFGA
EYANVQPHSGSQANTAVYHACLNPGDTILGFDLSHGGHLTHGSPVNFSGRLYRPVFYGVDAETGRLDYDKIQEIATKEQP
KLIIAGASAYSRDMDFARFRQIADSVGAILFADISHPAGLIAKGLLSDPIPHCHIVSTTTHKTLRGPRGGLILMGKDFPN
PQGLTTPKGEIRMMSSLLDLAVFPGNQGGPLMHIIAAKAVAFGEALKDEFFTYAMQLQKNANAMADAFVKRGYNIISGGT
DNHMMLIDLRNKNISGKEAENALVKAEITVNKNMVPFDDKSPFITSGIRVGTAAITTRGLVEKDMETIVALIDKVLTNHT
NEDVIEEVAEEVNELMSERPIFAY

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=403, Percent_Identity=47.6426799007444, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI261862352, Length=403, Percent_Identity=47.6426799007444, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862350, Length=403, Percent_Identity=47.6426799007444, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862348, Length=403, Percent_Identity=47.6426799007444, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=47.2772277227723, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI22547186, Length=402, Percent_Identity=45.771144278607, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI22547189, Length=400, Percent_Identity=41.75, Blast_Score=295, Evalue=7e-80,
Organism=Escherichia coli, GI1788902, Length=420, Percent_Identity=57.8571428571429, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=396, Percent_Identity=46.4646464646465, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=396, Percent_Identity=46.4646464646465, Blast_Score=342, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=414, Percent_Identity=42.7536231884058, Blast_Score=346, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=404, Percent_Identity=42.3267326732673, Blast_Score=315, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24640005, Length=408, Percent_Identity=48.0392156862745, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=406, Percent_Identity=48.2758620689655, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_FLAJ1 (A5FEF4)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195743.1
- ProteinModelPortal:   A5FEF4
- SMR:   A5FEF4
- STRING:   A5FEF4
- GeneID:   5093658
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3410
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   RPRMLIG
- ProtClustDB:   PRK00011
- BioCyc:   FJOH376686:FJOH_3410-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46391; Mature: 46391

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRDEQIFDLIQEEKDRQIHGLELIASENFVSDEVMEAAGSVLTNKYAEGYPGKRYYGGC
CCCHHHHHHHHHHHHCCCEECEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
EVVDVIEQIAIDRAKELFGAEYANVQPHSGSQANTAVYHACLNPGDTILGFDLSHGGHLT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEECCCCCEEEEEEECCCCCCC
HGSPVNFSGRLYRPVFYGVDAETGRLDYDKIQEIATKEQPKLIIAGASAYSRDMDFARFR
CCCCCCCCCEEECCEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHCCCHHHHHH
QIADSVGAILFADISHPAGLIAKGLLSDPIPHCHIVSTTTHKTLRGPRGGLILMGKDFPN
HHHHHHCEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCEEEEECCCCC
PQGLTTPKGEIRMMSSLLDLAVFPGNQGGPLMHIIAAKAVAFGEALKDEFFTYAMQLQKN
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANAMADAFVKRGYNIISGGTDNHMMLIDLRNKNISGKEAENALVKAEITVNKNMVPFDDK
CHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
SPFITSGIRVGTAAITTRGLVEKDMETIVALIDKVLTNHTNEDVIEEVAEEVNELMSERP
CCCEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
IFAY
CCCC
>Mature Secondary Structure
MQRDEQIFDLIQEEKDRQIHGLELIASENFVSDEVMEAAGSVLTNKYAEGYPGKRYYGGC
CCCHHHHHHHHHHHHCCCEECEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
EVVDVIEQIAIDRAKELFGAEYANVQPHSGSQANTAVYHACLNPGDTILGFDLSHGGHLT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEECCCCCEEEEEEECCCCCCC
HGSPVNFSGRLYRPVFYGVDAETGRLDYDKIQEIATKEQPKLIIAGASAYSRDMDFARFR
CCCCCCCCCEEECCEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHCCCHHHHHH
QIADSVGAILFADISHPAGLIAKGLLSDPIPHCHIVSTTTHKTLRGPRGGLILMGKDFPN
HHHHHHCEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCEEEEECCCCC
PQGLTTPKGEIRMMSSLLDLAVFPGNQGGPLMHIIAAKAVAFGEALKDEFFTYAMQLQKN
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANAMADAFVKRGYNIISGGTDNHMMLIDLRNKNISGKEAENALVKAEITVNKNMVPFDDK
CHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
SPFITSGIRVGTAAITTRGLVEKDMETIVALIDKVLTNHTNEDVIEEVAEEVNELMSERP
CCCEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
IFAY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA