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The map label for this gene is hisH [H]

Identifier: 146300623

GI number: 146300623

Start: 3410607

End: 3411188

Strand: Reverse

Name: hisH [H]

Synonym: Fjoh_2874

Alternate gene names: 146300623

Gene position: 3411188-3410607 (Counterclockwise)

Preceding gene: 146300624

Following gene: 146300622

Centisome position: 55.95

GC content: 34.88

Gene sequence:

>582_bases
ATGAAAATAGTAATTATAAATTACGGAGCAGGAAATATTCAGAGCATTATGTTTGCTATTGAAAGACTGGGTTTTAAGGC
GGTTTTGAGTAATAACCCGGACGAAATTAAATTGGCAGATAAAGTAATTTTTCCTGGCGTGGGAGAGGCGAGTTCGGCTA
TGGCAAAACTTCGTGAAAGTGGTTTAGATAGTCTGATTCCGCAATTGAAACAGCCCGTTTTAGGAATTTGTCTCGGAATG
CAGTTAATGTGCAATAAATCGGAAGAAGGAAACACAGAAGGTTTAGGAATTTTTGATGTTGATGTTTTGAAATTTTCAAA
CAATGTAAAAGTGCCGCAAATGGGATGGAATCAGATTTATGATTTAAAAACCGATTTGTTTAAAGGAATTTCTGAAAACG
AGTTCATGTATCTGGTTCATAGTTTTTACGCTCCAAATTGTGCTGAATCTATCGCAACAACAAATTACGATGTAGAATAC
GCATCGGCATTACAAAAAGATAATTTTTATGGAACCCAATTTCACCCAGAAAAAAGCGGTGATGTTGGAGAAAAGATTCT
AGGTAATTTTTTAAAAATGTAA

Upstream 100 bases:

>100_bases
AGTCGAAAGTTGAAAGTCATAAAGTCAAAAGATTCCTAAATTATGGATTTCAGAAAGCGACTTTAAGACTTTGGACTTTA
GACTTTAAGACTAAAATTAA

Downstream 100 bases:

>100_bases
AATTTCAATATCAAAAATCAATCAAAATATCAATTTTTGAGAATCAAAAAGACTTTAAGACTTTAGACTTTCAAACTTTA
AGACTAAAATAAAATGAGAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKIVIINYGAGNIQSIMFAIERLGFKAVLSNNPDEIKLADKVIFPGVGEASSAMAKLRESGLDSLIPQLKQPVLGICLGM
QLMCNKSEEGNTEGLGIFDVDVLKFSNNVKVPQMGWNQIYDLKTDLFKGISENEFMYLVHSFYAPNCAESIATTNYDVEY
ASALQKDNFYGTQFHPEKSGDVGEKILGNFLKM

Sequences:

>Translated_193_residues
MKIVIINYGAGNIQSIMFAIERLGFKAVLSNNPDEIKLADKVIFPGVGEASSAMAKLRESGLDSLIPQLKQPVLGICLGM
QLMCNKSEEGNTEGLGIFDVDVLKFSNNVKVPQMGWNQIYDLKTDLFKGISENEFMYLVHSFYAPNCAESIATTNYDVEY
ASALQKDNFYGTQFHPEKSGDVGEKILGNFLKM
>Mature_193_residues
MKIVIINYGAGNIQSIMFAIERLGFKAVLSNNPDEIKLADKVIFPGVGEASSAMAKLRESGLDSLIPQLKQPVLGICLGM
QLMCNKSEEGNTEGLGIFDVDVLKFSNNVKVPQMGWNQIYDLKTDLFKGISENEFMYLVHSFYAPNCAESIATTNYDVEY
ASALQKDNFYGTQFHPEKSGDVGEKILGNFLKM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=47.4489795918367, Blast_Score=183, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=33.1730769230769, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 21350; Mature: 21350

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIINYGAGNIQSIMFAIERLGFKAVLSNNPDEIKLADKVIFPGVGEASSAMAKLRES
CEEEEEECCCCHHHHHHHHHHHHCHHEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHC
GLDSLIPQLKQPVLGICLGMQLMCNKSEEGNTEGLGIFDVDVLKFSNNVKVPQMGWNQIY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHH
DLKTDLFKGISENEFMYLVHSFYAPNCAESIATTNYDVEYASALQKDNFYGTQFHPEKSG
HHHHHHHCCCCCCCCEEEEHHHCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
DVGEKILGNFLKM
CHHHHHHHHHHCC
>Mature Secondary Structure
MKIVIINYGAGNIQSIMFAIERLGFKAVLSNNPDEIKLADKVIFPGVGEASSAMAKLRES
CEEEEEECCCCHHHHHHHHHHHHCHHEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHC
GLDSLIPQLKQPVLGICLGMQLMCNKSEEGNTEGLGIFDVDVLKFSNNVKVPQMGWNQIY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHH
DLKTDLFKGISENEFMYLVHSFYAPNCAESIATTNYDVEYASALQKDNFYGTQFHPEKSG
HHHHHHHCCCCCCCCEEEEHHHCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
DVGEKILGNFLKM
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA