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The map label for this gene is hisA

Identifier: 146300622

GI number: 146300622

Start: 3409791

End: 3410513

Strand: Reverse

Name: hisA

Synonym: Fjoh_2873

Alternate gene names: 146300622

Gene position: 3410513-3409791 (Counterclockwise)

Preceding gene: 146300623

Following gene: 146300621

Centisome position: 55.94

GC content: 33.89

Gene sequence:

>723_bases
ATGAGAATAATACCAGCCATAGATATCATTGAAGGAAAATGCGTTCGTTTGTCCAAAGGTGATTATGATACCAAAATAAT
TTACAATGAAAATCCGCTTGAAGTAGCGAAATCATTTGAAGCATACGGAATTGAATATTTACATTTAGTAGATCTTGATG
GTGCAAAATCAAGTAAAATTGTCAATTATAAAATATTAGAACAAATTGCAACGCAAACGAGCTTAAAAATTGATTTTGGA
GGCGGATTAAAGTCGGATGATGATTTGAGAATTGCTTTTGAAAGCGGTGCAAACCAAATTACCGGCGGAAGTATTGCTGT
AAAAAACAGAACGATTTTCGAAAAATGGATTTCAGAATACGGTTCAGAAAAAATTATTTTGGGAGCTGATGCAAAAGATG
AAAAAATTGCGGTTTCTGGCTGGTTAGAAGAATCAAATGAAGATTTAGTTCCGTTTATTCAGGATTATCAAAATAAAGGA
ATTCAATACGTTATTTGCACCGATATCGCCAAAGACGGAATGCTGCAAGGTCCAAGTTTTGATTTGTACAGTAAAATTTT
AGCAGAAGCTAAAGGCGTAAAACTAATCGCTTCTGGAGGAATCTCAACTTTTGACGAATTGCCAAAATTAGCCGAATTAG
GTTGTGAAGGAACGATTATAGGAAAAGCAATTTACGAAGGAAGAATTACCTTAAAGCAGTTAGAGAATTACATAATAGGT
TGA

Upstream 100 bases:

>100_bases
AATGTAAAATTTCAATATCAAAAATCAATCAAAATATCAATTTTTGAGAATCAAAAAGACTTTAAGACTTTAGACTTTCA
AACTTTAAGACTAAAATAAA

Downstream 100 bases:

>100_bases
TAGTTATTGGTTGTCGCTCGATAGTTTATCATATTGCCCAACAACCAACAACCAACAACCAACAACCAAATAAAAAATGC
TAGCAAAAAGAATAATACCC

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRIIPAIDIIEGKCVRLSKGDYDTKIIYNENPLEVAKSFEAYGIEYLHLVDLDGAKSSKIVNYKILEQIATQTSLKIDFG
GGLKSDDDLRIAFESGANQITGGSIAVKNRTIFEKWISEYGSEKIILGADAKDEKIAVSGWLEESNEDLVPFIQDYQNKG
IQYVICTDIAKDGMLQGPSFDLYSKILAEAKGVKLIASGGISTFDELPKLAELGCEGTIIGKAIYEGRITLKQLENYIIG

Sequences:

>Translated_240_residues
MRIIPAIDIIEGKCVRLSKGDYDTKIIYNENPLEVAKSFEAYGIEYLHLVDLDGAKSSKIVNYKILEQIATQTSLKIDFG
GGLKSDDDLRIAFESGANQITGGSIAVKNRTIFEKWISEYGSEKIILGADAKDEKIAVSGWLEESNEDLVPFIQDYQNKG
IQYVICTDIAKDGMLQGPSFDLYSKILAEAKGVKLIASGGISTFDELPKLAELGCEGTIIGKAIYEGRITLKQLENYIIG
>Mature_240_residues
MRIIPAIDIIEGKCVRLSKGDYDTKIIYNENPLEVAKSFEAYGIEYLHLVDLDGAKSSKIVNYKILEQIATQTSLKIDFG
GGLKSDDDLRIAFESGANQITGGSIAVKNRTIFEKWISEYGSEKIILGADAKDEKIAVSGWLEESNEDLVPFIQDYQNKG
IQYVICTDIAKDGMLQGPSFDLYSKILAEAKGVKLIASGGISTFDELPKLAELGCEGTIIGKAIYEGRITLKQLENYIIG

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=236, Percent_Identity=34.7457627118644, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1788336, Length=248, Percent_Identity=23.3870967741935, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_FLAJ1 (A5FFX7)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195213.1
- ProteinModelPortal:   A5FFX7
- SMR:   A5FFX7
- STRING:   A5FFX7
- GeneID:   5092693
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2873
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   LLAINGW
- ProtClustDB:   CLSK822682
- BioCyc:   FJOH376686:FJOH_2873-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26371; Mature: 26371

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIPAIDIIEGKCVRLSKGDYDTKIIYNENPLEVAKSFEAYGIEYLHLVDLDGAKSSKI
CCCCCCEEEECCCEEEECCCCCCEEEEECCCHHHHHHHHHHCCEEEEEEEECCCCCCCEE
VNYKILEQIATQTSLKIDFGGGLKSDDDLRIAFESGANQITGGSIAVKNRTIFEKWISEY
EHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCCCEECCCEEEEECCHHHHHHHHHH
GSEKIILGADAKDEKIAVSGWLEESNEDLVPFIQDYQNKGIQYVICTDIAKDGMLQGPSF
CCCEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
DLYSKILAEAKGVKLIASGGISTFDELPKLAELGCEGTIIGKAIYEGRITLKQLENYIIG
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MRIIPAIDIIEGKCVRLSKGDYDTKIIYNENPLEVAKSFEAYGIEYLHLVDLDGAKSSKI
CCCCCCEEEECCCEEEECCCCCCEEEEECCCHHHHHHHHHHCCEEEEEEEECCCCCCCEE
VNYKILEQIATQTSLKIDFGGGLKSDDDLRIAFESGANQITGGSIAVKNRTIFEKWISEY
EHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCCCEECCCEEEEECCHHHHHHHHHH
GSEKIILGADAKDEKIAVSGWLEESNEDLVPFIQDYQNKGIQYVICTDIAKDGMLQGPSF
CCCEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
DLYSKILAEAKGVKLIASGGISTFDELPKLAELGCEGTIIGKAIYEGRITLKQLENYIIG
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA