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The map label for this gene is hisF

Identifier: 146300621

GI number: 146300621

Start: 3408959

End: 3409714

Strand: Reverse

Name: hisF

Synonym: Fjoh_2872

Alternate gene names: 146300621

Gene position: 3409714-3408959 (Counterclockwise)

Preceding gene: 146300622

Following gene: 146300620

Centisome position: 55.93

GC content: 38.62

Gene sequence:

>756_bases
ATGCTAGCAAAAAGAATAATACCCTGCTTAGATATAAAGAACGGAAGAACTGTAAAAGGCGTTAATTTCGTTGATTTGCG
TGATGCAGGCGATCCTGTAGAACTGGCTGAAATTTATTCGAGAGAAGGTGCAGACGAATTGGTTTTCCTGGATATTTCGG
CAACTGAGGAAAGACGTAAAACGCTGGTAAATATGGTGCGAAGCGTGGCAGAAAAAATCAACATTCCGTTTACTGTTGGC
GGTGGGATTTCATCTGTTGAAGATGTTGATATTCTGCTGAATAATGGAGCTGATAAAGTTTCAATTAATTCATCGGCAGT
TAAAAATCCGCAGTTGATTAATGATTTGGCTCAGAAATTTGGAAGTCAGTGTGTTGTTGTAGCAATCGATGCTAAACAAA
TTGACGGACAATGGATTGTACATTTAGTCGGCGGAAAAGTTCCGACTGAATTGAATTTATTCGATTGGGCTGTTGAAGTG
GCAGAACGAGGAGCAGGAGAAATCCTTTTTACTTCGATGGATAATGACGGAACTAAAAATGGCTTTGCAAACGAAGCTCT
GGCAAAACTATCAGAATTAGTAAATATTCCAATTATTGCTTCGGGAGGCGCTGGAAACATACAGCATTTCGTAGATTCGT
TTAAAGAAGGAAAAGCAGACGCCGCTTTGGCTGCAAGTGTTTTTCATTTTAAAGAAATCGAAATCAAAGCATTAAAGCAA
GAATTAAGAAAGAATGGTGTTGAAGTTAGACTTTAA

Upstream 100 bases:

>100_bases
TTAGAGAATTACATAATAGGTTGATAGTTATTGGTTGTCGCTCGATAGTTTATCATATTGCCCAACAACCAACAACCAAC
AACCAACAACCAAATAAAAA

Downstream 100 bases:

>100_bases
GAGAAGCAGGAAACAAGAAGAAAGAGAAGATTTAATCTAAAATAAAAGAGTTCAGCAATCTAAAATCTGAATTCTAAAAT
CTAAAATAATTATGGATATC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDIKNGRTVKGVNFVDLRDAGDPVELAEIYSREGADELVFLDISATEERRKTLVNMVRSVAEKINIPFTVG
GGISSVEDVDILLNNGADKVSINSSAVKNPQLINDLAQKFGSQCVVVAIDAKQIDGQWIVHLVGGKVPTELNLFDWAVEV
AERGAGEILFTSMDNDGTKNGFANEALAKLSELVNIPIIASGGAGNIQHFVDSFKEGKADAALAASVFHFKEIEIKALKQ
ELRKNGVEVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDIKNGRTVKGVNFVDLRDAGDPVELAEIYSREGADELVFLDISATEERRKTLVNMVRSVAEKINIPFTVG
GGISSVEDVDILLNNGADKVSINSSAVKNPQLINDLAQKFGSQCVVVAIDAKQIDGQWIVHLVGGKVPTELNLFDWAVEV
AERGAGEILFTSMDNDGTKNGFANEALAKLSELVNIPIIASGGAGNIQHFVDSFKEGKADAALAASVFHFKEIEIKALKQ
ELRKNGVEVRL
>Mature_251_residues
MLAKRIIPCLDIKNGRTVKGVNFVDLRDAGDPVELAEIYSREGADELVFLDISATEERRKTLVNMVRSVAEKINIPFTVG
GGISSVEDVDILLNNGADKVSINSSAVKNPQLINDLAQKFGSQCVVVAIDAKQIDGQWIVHLVGGKVPTELNLFDWAVEV
AERGAGEILFTSMDNDGTKNGFANEALAKLSELVNIPIIASGGAGNIQHFVDSFKEGKADAALAASVFHFKEIEIKALKQ
ELRKNGVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=48.6381322957198, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=28.9099526066351, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=29.6178343949045, Blast_Score=138, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_FLAJ1 (A5FFX6)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195212.1
- ProteinModelPortal:   A5FFX6
- SMR:   A5FFX6
- STRING:   A5FFX6
- GeneID:   5090501
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2872
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   FJOH376686:FJOH_2872-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27113; Mature: 27113

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDIKNGRTVKGVNFVDLRDAGDPVELAEIYSREGADELVFLDISATEERRK
CCHHHCCCCEECCCCCEECCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHH
TLVNMVRSVAEKINIPFTVGGGISSVEDVDILLNNGADKVSINSSAVKNPQLINDLAQKF
HHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCCCEEEECCHHCCCHHHHHHHHHHH
GSQCVVVAIDAKQIDGQWIVHLVGGKVPTELNLFDWAVEVAERGAGEILFTSMDNDGTKN
CCCEEEEEEECEECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GFANEALAKLSELVNIPIIASGGAGNIQHFVDSFKEGKADAALAASVFHFKEIEIKALKQ
CHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ELRKNGVEVRL
HHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDIKNGRTVKGVNFVDLRDAGDPVELAEIYSREGADELVFLDISATEERRK
CCHHHCCCCEECCCCCEECCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHH
TLVNMVRSVAEKINIPFTVGGGISSVEDVDILLNNGADKVSINSSAVKNPQLINDLAQKF
HHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCCCEEEECCHHCCCHHHHHHHHHHH
GSQCVVVAIDAKQIDGQWIVHLVGGKVPTELNLFDWAVEVAERGAGEILFTSMDNDGTKN
CCCEEEEEEECEECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GFANEALAKLSELVNIPIIASGGAGNIQHFVDSFKEGKADAALAASVFHFKEIEIKALKQ
CHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ELRKNGVEVRL
HHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA