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The map label for this gene is tilS [H]

Identifier: 146299894

GI number: 146299894

Start: 2587887

End: 2589197

Strand: Direct

Name: tilS [H]

Synonym: Fjoh_2138

Alternate gene names: 146299894

Gene position: 2587887-2589197 (Clockwise)

Preceding gene: 146299893

Following gene: 146299895

Centisome position: 42.45

GC content: 32.19

Gene sequence:

>1311_bases
ATGCTTTCAAAATTTCAAAATCATATCGTATCCAAATTTCCTTTCTTAAAACAGAAAAAGCTTTTTCTGGCTGTAAGCGG
AGGACTAGACAGTATGGTTTTACTGCATTTGTTTAAGCAGTTAAACTATGAAATTACTGTTTTACATTGTAATTTTCAGC
TTAGGGGATTGGAAAGTTTTGGCGATCAGGAATTTATTCAAAATTATTGTGATAAAAATAATATCAAAATTTTTACTACT
CAATTTGATACTCAGGCGTTTGCAGAAGATTATAAGCTTTCGATACAAGTTGCGGCAAGAGAACTTCGATACAGTTGGTT
TTATGAGCTTTTAGAAGAAAAACACTTCGATTATCTCTTAACTGCCCATCATGCCGATGATAATCTGGAAACTTTTATCA
TCAATTTAACCCGCGGAACCGGATTAGACGGCTTAACTGGAATTCCGGAACAAAACGATAAAATTATTCGTCCATTACTT
CCATTTTCAAGAGAAGAAATTTTAAAATACGCTCAGGAAAATAATATAGAATGGCGTGAGGACAGCAGTAATGCTTCGAC
TAAATATGTTCGAAATAAAATTCGTCATGATTTGGTTCCGGTTTTAAAACAAATCAATCCTAATTTTTTGGATGCTTTTC
AAAAAACACAATCTTTTCTGCAGGAATCAAAAGAAATGGTAGAAGATGCTTCGATTGTGATTTATCAGCAAGTTGCCAAA
GAAGCCGGAGACGACATTCATTTTGATTTAAACCAGCTTAAAAAACTTCCAAATTATAAATCGTATTTGTATCAATGGCT
GAATGAATTTGGTTTTTCGGCATGGAAAGATATTTATGATTTGGTTGAAGGACAATCCGGGAAACAAGTTTTTTCAGATG
AATTCAGACTGCTGAAAAATCGCGAAATTTTGATTTTGACTCCAATTTCAGAAACATCAGAAAAAGAAGAATTTGAAATT
TATGAAAACGATACAGAAGTTAATTTTCCCTTAAAAATGACACTTTGTAACGTAGGTCACACAACATTCGGTTCAAATAG
AACTATATTTGTCGACGCTGAAAAAATCCAGTTTCCTTTGAAATTACGTAAATGGAATGAAGGCGATGTTTTTCAGCCTT
TTGGAATGAATGGTAAGTCTAAAAAAGTAAGCAAACTTTTTAAAGACGAAAAAATGTCCCTGATCGAAAAAGAAAAAACA
TGGATTTTGTCTTCTGATGATCAAATAGTCTGGATTGTTGGAATTAGACAGGACGAACGTTTTAAAATAAATAATACCAC
AAATAAAATACTTAAAATAGAATTGCAATAA

Upstream 100 bases:

>100_bases
TTTTATTGCCTGTATTTTAAAGGGAAAAATTCGTGGCAGAAAAAATCCGCTTAACATTTCATTTAAAAATAGATCGAATT
TAATTTTTTACTTTTATCGA

Downstream 100 bases:

>100_bases
TGAACTTTAATCAAAATCATCGAGCGATACTTTCAAGAAGTATCTGGAATAAAATAACCGCCTTTTTACTGCTGTTCTTT
TTTACTTTAGCAGGAAATGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLSKFQNHIVSKFPFLKQKKLFLAVSGGLDSMVLLHLFKQLNYEITVLHCNFQLRGLESFGDQEFIQNYCDKNNIKIFTT
QFDTQAFAEDYKLSIQVAARELRYSWFYELLEEKHFDYLLTAHHADDNLETFIINLTRGTGLDGLTGIPEQNDKIIRPLL
PFSREEILKYAQENNIEWREDSSNASTKYVRNKIRHDLVPVLKQINPNFLDAFQKTQSFLQESKEMVEDASIVIYQQVAK
EAGDDIHFDLNQLKKLPNYKSYLYQWLNEFGFSAWKDIYDLVEGQSGKQVFSDEFRLLKNREILILTPISETSEKEEFEI
YENDTEVNFPLKMTLCNVGHTTFGSNRTIFVDAEKIQFPLKLRKWNEGDVFQPFGMNGKSKKVSKLFKDEKMSLIEKEKT
WILSSDDQIVWIVGIRQDERFKINNTTNKILKIELQ

Sequences:

>Translated_436_residues
MLSKFQNHIVSKFPFLKQKKLFLAVSGGLDSMVLLHLFKQLNYEITVLHCNFQLRGLESFGDQEFIQNYCDKNNIKIFTT
QFDTQAFAEDYKLSIQVAARELRYSWFYELLEEKHFDYLLTAHHADDNLETFIINLTRGTGLDGLTGIPEQNDKIIRPLL
PFSREEILKYAQENNIEWREDSSNASTKYVRNKIRHDLVPVLKQINPNFLDAFQKTQSFLQESKEMVEDASIVIYQQVAK
EAGDDIHFDLNQLKKLPNYKSYLYQWLNEFGFSAWKDIYDLVEGQSGKQVFSDEFRLLKNREILILTPISETSEKEEFEI
YENDTEVNFPLKMTLCNVGHTTFGSNRTIFVDAEKIQFPLKLRKWNEGDVFQPFGMNGKSKKVSKLFKDEKMSLIEKEKT
WILSSDDQIVWIVGIRQDERFKINNTTNKILKIELQ
>Mature_436_residues
MLSKFQNHIVSKFPFLKQKKLFLAVSGGLDSMVLLHLFKQLNYEITVLHCNFQLRGLESFGDQEFIQNYCDKNNIKIFTT
QFDTQAFAEDYKLSIQVAARELRYSWFYELLEEKHFDYLLTAHHADDNLETFIINLTRGTGLDGLTGIPEQNDKIIRPLL
PFSREEILKYAQENNIEWREDSSNASTKYVRNKIRHDLVPVLKQINPNFLDAFQKTQSFLQESKEMVEDASIVIYQQVAK
EAGDDIHFDLNQLKKLPNYKSYLYQWLNEFGFSAWKDIYDLVEGQSGKQVFSDEFRLLKNREILILTPISETSEKEEFEI
YENDTEVNFPLKMTLCNVGHTTFGSNRTIFVDAEKIQFPLKLRKWNEGDVFQPFGMNGKSKKVSKLFKDEKMSLIEKEKT
WILSSDDQIVWIVGIRQDERFKINNTTNKILKIELQ

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=219, Percent_Identity=31.9634703196347, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51225; Mature: 51225

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKFQNHIVSKFPFLKQKKLFLAVSGGLDSMVLLHLFKQLNYEITVLHCNFQLRGLESF
CCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEECEEEECCHHC
GDQEFIQNYCDKNNIKIFTTQFDTQAFAEDYKLSIQVAARELRYSWFYELLEEKHFDYLL
CCHHHHHHHCCCCCEEEEEEECCHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCEEE
TAHHADDNLETFIINLTRGTGLDGLTGIPEQNDKIIRPLLPFSREEILKYAQENNIEWRE
EEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCC
DSSNASTKYVRNKIRHDLVPVLKQINPNFLDAFQKTQSFLQESKEMVEDASIVIYQQVAK
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGDDIHFDLNQLKKLPNYKSYLYQWLNEFGFSAWKDIYDLVEGQSGKQVFSDEFRLLKN
HCCCCEEECHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
REILILTPISETSEKEEFEIYENDTEVNFPLKMTLCNVGHTTFGSNRTIFVDAEKIQFPL
CCEEEEECCCCCCCCCCEEEEECCCEECCEEEEEEEECCCCCCCCCCEEEEEHHHEEECE
KLRKWNEGDVFQPFGMNGKSKKVSKLFKDEKMSLIEKEKTWILSSDDQIVWIVGIRQDER
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCC
FKINNTTNKILKIELQ
EEEECCCCEEEEEEEC
>Mature Secondary Structure
MLSKFQNHIVSKFPFLKQKKLFLAVSGGLDSMVLLHLFKQLNYEITVLHCNFQLRGLESF
CCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEECEEEECCHHC
GDQEFIQNYCDKNNIKIFTTQFDTQAFAEDYKLSIQVAARELRYSWFYELLEEKHFDYLL
CCHHHHHHHCCCCCEEEEEEECCHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCEEE
TAHHADDNLETFIINLTRGTGLDGLTGIPEQNDKIIRPLLPFSREEILKYAQENNIEWRE
EEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCC
DSSNASTKYVRNKIRHDLVPVLKQINPNFLDAFQKTQSFLQESKEMVEDASIVIYQQVAK
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGDDIHFDLNQLKKLPNYKSYLYQWLNEFGFSAWKDIYDLVEGQSGKQVFSDEFRLLKN
HCCCCEEECHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
REILILTPISETSEKEEFEIYENDTEVNFPLKMTLCNVGHTTFGSNRTIFVDAEKIQFPL
CCEEEEECCCCCCCCCCEEEEECCCEECCEEEEEEEECCCCCCCCCCEEEEEHHHEEECE
KLRKWNEGDVFQPFGMNGKSKKVSKLFKDEKMSLIEKEKTWILSSDDQIVWIVGIRQDER
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCC
FKINNTTNKILKIELQ
EEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA