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The map label for this gene is topA [H]

Identifier: 146299611

GI number: 146299611

Start: 2132134

End: 2134653

Strand: Direct

Name: topA [H]

Synonym: Fjoh_1851

Alternate gene names: 146299611

Gene position: 2132134-2134653 (Clockwise)

Preceding gene: 146299600

Following gene: 146299612

Centisome position: 34.97

GC content: 36.59

Gene sequence:

>2520_bases
ATGGCAAAGAATTTAGTAATAGTGGAGTCACCTGCAAAGGCGAAAACGATCGAGAAATTTCTTGGGAGTGATTTTCAGGT
GGAGTCAAGTTATGGTCACATAGCAGACTTACCATCCAAGGAAATAGGGGTAGATGTAGAGAATGGTTTTAAACCTAAAT
ATGAAGTTTCCCCGGATAAAAAAGCCTTGGTAACGAAACTAAAATCGCTATCTAAAAATGCCGAAACGGTTTGGTTAGCA
TCCGATGAGGACCGCGAGGGAGAGGCTATTTCATGGCACTTGGCGGAAGAATTAAAACTGGATACAAAAAAGACCAAACG
AATTGTTTTTCACGAGATTACAAAATCTGCGATTCTTAAAGCAATTGACAATCCAAGAGAAATTGATTATAATTTAGTTA
ATGCGCAGCAAGCGAGAAGAGTATTAGATCGTTTAGTGGGTTATGAATTATCTCCGGTTTTATGGAGAAAAATTAAAGGC
GGACTTTCTGCAGGTCGTGTACAATCTGTTTCTGTACGTTTGATTGTTGAAAGAGAACGCGAAATTCAAAGTTTTAATGC
AGTGGCAACCTATTCAATTGTTGCTGAATTTGTAAACGAAGCTGGAAAAGCTTTTAAAGCAAAACTGCCAAAAAACTTCA
ATACAAAAAAAGAAGCCGAAGATTTTTTAAACAAAAACATCGGTTCTAAATATAAGGTAGCTGATTTAGAAACTAAACCT
ACCAAAAAATCTCCAACAGCACCTTTTACAACTTCAACGTTGCAGCAGGAAGCAGCAAGAAAATTATACCTGCCGGTTGG
AATTACCATGCAGTTAGCACAGCGTTTATATGAGGCGGGACTTATTACTTATATGAGAACCGACTCGGTAAATCTTTCTA
AAGAAGCAATGGATGCTGCACAAGCAGAAATCATAAAATCTTACGGGGAAGAATTTTCTAAACCGAGAATTTTTGCAACA
AAAAGTAAAGGAGCACAAGAAGCGCACGAAGCAATTCGTCCGACTGATATGTCACGCCATACTGTAAATATCGATCGTGA
TCAGGCTCGTTTGTATGATTTGATCTGGAAAAGAACTTTAGCATCTCAAATGAGCGATGCGCAGCTTGAAAGAACAAACG
TAAAAATAGAAGCTGATAATCATGATGAGCTTTTTACAGCTTCTGGAGAAGTTTTGCTTTTTGAAGGTTTCTTAAAAGTG
TATTTAGAAGGTCATGATGATGATGAGGAAGAGCAAGAAGGAATGCTTCCGGCTTTAAAAGTAAACGAAAAGCTGGCTAA
TAACTATATTACAGCTACAGAACGATATTCAAGACCGCCTGCACGTTATACAGAGGCATCTTTGGTTAAAAAACTAGAGG
AACTTGGTATCGGACGTCCGTCTACTTATGCACCAACTATTTCAACAATTATCAATAGAAATTATGTTGAAAAAGGAACG
CTTGAAGGGCAGGAACGTAACTATACACAGTTGACTTTACAAAATAGTAAAGTAGGTGAGAAGCTTTTAAAAGAAAATAC
TGGTTCTGATAAAGGAAAGCTGGTTCCTACTGATATTGGTACTATCGTAACAGATTTCTTAGTTAAGAATTTCGGGAATA
TTTTAGATTATAATTTTACTGCAAAAGTTGAGCAGGATTTTGATGAAATTGCCGAAGGAAATATCGACTGGGCAATCATG
ATGCAGGATTTCTACAATAAATTTCACCCAAATGTAAAAGAAGTTGAAGCTAATGCTGAACGTGAAAGCGGTGAAAGAAT
TTTAGGAAAAGATGCAGATGGAAGACAAGTTTCTGTTCGTTTAGGGAAATTTGGACCAATGGCTCAAATTGGAGAAGCAG
ATGATGAAGATAAAAAGTTTGCCAGCCTGATGGCAGATCAAAATATTGGAAATATCACACTTGAAGAAGCTTTGAATTTA
TTCTTGCTTCCTAAAAATTTAGGAGAATATAAAGGAGAAGAAGTAGAAGTGAGCAATGGTCGTTACGGTCCGTATGTTCG
TCACGGAAGTGTGTTTATTTCATTACCTCGCGGTGAAGATCCTTTGAATGTTACAAAAGAAAGAGCTAAGGAATTAATTG
ACGAAAAAGCACTTGCTGATGCGCCAATTGCTGTTTACAAAGGAGAAGCAGTTCAAAAAGGTGTGGGACGTTTTGGTCCT
TTTATTAAATGGAACGGTCTTTTTGTAAATGTGAGCAAAAAATACAATTTCGATAATTTATCTCAGGCAGATGTTGAAGA
ATTAATTGAAGATAAATTACAAAAGAATATTGATAAAGTTCTTCACAATTGGGAAGAAGAAGGAATTGTAGTTGAAAAAG
CCCGCTGGGGACGTTCAGTTATTTTAAAAGGTAAAATCAAAATTGAATTAAGTAAAGATGTTGATGCGACAAAATTAACA
TTGGCCGAAGTTCAGGAAATGATCGAGAAAAAAGCACCGGCTAAAAAAACAGCAGCAAAGAAAACCACAGCAGCTAAAAA
AACTACAACCGCTAAAAAAGCACCAGCAAAAAAGAAATAA

Upstream 100 bases:

>100_bases
CTGCAATTTTAAGTTTCATAATATCAAAATACCTCAAAAATGCATTCGTAAGGTTAATATTTAAAAAAAATAAATACTTT
TGTTGCAGAAAATAAGAGCA

Downstream 100 bases:

>100_bases
AGAATGGAATTTGACTTTCTAGAGCCTTTAAATGACGGAATTTTAAAATTCATTAGTTCATTGTCTACACAAGAACTGGG
GAGCAAAATTGTTTTGCATA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 839; Mature: 838

Protein sequence:

>839_residues
MAKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGVDVENGFKPKYEVSPDKKALVTKLKSLSKNAETVWLA
SDEDREGEAISWHLAEELKLDTKKTKRIVFHEITKSAILKAIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWRKIKG
GLSAGRVQSVSVRLIVEREREIQSFNAVATYSIVAEFVNEAGKAFKAKLPKNFNTKKEAEDFLNKNIGSKYKVADLETKP
TKKSPTAPFTTSTLQQEAARKLYLPVGITMQLAQRLYEAGLITYMRTDSVNLSKEAMDAAQAEIIKSYGEEFSKPRIFAT
KSKGAQEAHEAIRPTDMSRHTVNIDRDQARLYDLIWKRTLASQMSDAQLERTNVKIEADNHDELFTASGEVLLFEGFLKV
YLEGHDDDEEEQEGMLPALKVNEKLANNYITATERYSRPPARYTEASLVKKLEELGIGRPSTYAPTISTIINRNYVEKGT
LEGQERNYTQLTLQNSKVGEKLLKENTGSDKGKLVPTDIGTIVTDFLVKNFGNILDYNFTAKVEQDFDEIAEGNIDWAIM
MQDFYNKFHPNVKEVEANAERESGERILGKDADGRQVSVRLGKFGPMAQIGEADDEDKKFASLMADQNIGNITLEEALNL
FLLPKNLGEYKGEEVEVSNGRYGPYVRHGSVFISLPRGEDPLNVTKERAKELIDEKALADAPIAVYKGEAVQKGVGRFGP
FIKWNGLFVNVSKKYNFDNLSQADVEELIEDKLQKNIDKVLHNWEEEGIVVEKARWGRSVILKGKIKIELSKDVDATKLT
LAEVQEMIEKKAPAKKTAAKKTTAAKKTTTAKKAPAKKK

Sequences:

>Translated_839_residues
MAKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGVDVENGFKPKYEVSPDKKALVTKLKSLSKNAETVWLA
SDEDREGEAISWHLAEELKLDTKKTKRIVFHEITKSAILKAIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWRKIKG
GLSAGRVQSVSVRLIVEREREIQSFNAVATYSIVAEFVNEAGKAFKAKLPKNFNTKKEAEDFLNKNIGSKYKVADLETKP
TKKSPTAPFTTSTLQQEAARKLYLPVGITMQLAQRLYEAGLITYMRTDSVNLSKEAMDAAQAEIIKSYGEEFSKPRIFAT
KSKGAQEAHEAIRPTDMSRHTVNIDRDQARLYDLIWKRTLASQMSDAQLERTNVKIEADNHDELFTASGEVLLFEGFLKV
YLEGHDDDEEEQEGMLPALKVNEKLANNYITATERYSRPPARYTEASLVKKLEELGIGRPSTYAPTISTIINRNYVEKGT
LEGQERNYTQLTLQNSKVGEKLLKENTGSDKGKLVPTDIGTIVTDFLVKNFGNILDYNFTAKVEQDFDEIAEGNIDWAIM
MQDFYNKFHPNVKEVEANAERESGERILGKDADGRQVSVRLGKFGPMAQIGEADDEDKKFASLMADQNIGNITLEEALNL
FLLPKNLGEYKGEEVEVSNGRYGPYVRHGSVFISLPRGEDPLNVTKERAKELIDEKALADAPIAVYKGEAVQKGVGRFGP
FIKWNGLFVNVSKKYNFDNLSQADVEELIEDKLQKNIDKVLHNWEEEGIVVEKARWGRSVILKGKIKIELSKDVDATKLT
LAEVQEMIEKKAPAKKTAAKKTTAAKKTTTAKKAPAKKK
>Mature_838_residues
AKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGVDVENGFKPKYEVSPDKKALVTKLKSLSKNAETVWLAS
DEDREGEAISWHLAEELKLDTKKTKRIVFHEITKSAILKAIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWRKIKGG
LSAGRVQSVSVRLIVEREREIQSFNAVATYSIVAEFVNEAGKAFKAKLPKNFNTKKEAEDFLNKNIGSKYKVADLETKPT
KKSPTAPFTTSTLQQEAARKLYLPVGITMQLAQRLYEAGLITYMRTDSVNLSKEAMDAAQAEIIKSYGEEFSKPRIFATK
SKGAQEAHEAIRPTDMSRHTVNIDRDQARLYDLIWKRTLASQMSDAQLERTNVKIEADNHDELFTASGEVLLFEGFLKVY
LEGHDDDEEEQEGMLPALKVNEKLANNYITATERYSRPPARYTEASLVKKLEELGIGRPSTYAPTISTIINRNYVEKGTL
EGQERNYTQLTLQNSKVGEKLLKENTGSDKGKLVPTDIGTIVTDFLVKNFGNILDYNFTAKVEQDFDEIAEGNIDWAIMM
QDFYNKFHPNVKEVEANAERESGERILGKDADGRQVSVRLGKFGPMAQIGEADDEDKKFASLMADQNIGNITLEEALNLF
LLPKNLGEYKGEEVEVSNGRYGPYVRHGSVFISLPRGEDPLNVTKERAKELIDEKALADAPIAVYKGEAVQKGVGRFGPF
IKWNGLFVNVSKKYNFDNLSQADVEELIEDKLQKNIDKVLHNWEEEGIVVEKARWGRSVILKGKIKIELSKDVDATKLTL
AEVQEMIEKKAPAKKTAAKKTTAAKKTTTAKKAPAKKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=434, Percent_Identity=27.6497695852535, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI4507635, Length=552, Percent_Identity=24.2753623188406, Blast_Score=97, Evalue=8e-20,
Organism=Escherichia coli, GI1787529, Length=656, Percent_Identity=39.4817073170732, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1788061, Length=481, Percent_Identity=25.987525987526, Blast_Score=106, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=451, Percent_Identity=25.4988913525499, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=522, Percent_Identity=25.095785440613, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=570, Percent_Identity=25.2631578947368, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585251, Length=485, Percent_Identity=26.8041237113402, Blast_Score=107, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24640096, Length=500, Percent_Identity=24.6, Blast_Score=94, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 94104; Mature: 93973

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGVDVENGFKPKYEVSPDK
CCCCEEEEECCCHHHHHHHHCCCCEEEECCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCH
KALVTKLKSLSKNAETVWLASDEDREGEAISWHLAEELKLDTKKTKRIVFHEITKSAILK
HHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHH
AIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWRKIKGGLSAGRVQSVSVRLIVERER
HCCCCHHCCEEHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEEHHH
EIQSFNAVATYSIVAEFVNEAGKAFKAKLPKNFNTKKEAEDFLNKNIGSKYKVADLETKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCC
TKKSPTAPFTTSTLQQEAARKLYLPVGITMQLAQRLYEAGLITYMRTDSVNLSKEAMDAA
CCCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
QAEIIKSYGEEFSKPRIFATKSKGAQEAHEAIRPTDMSRHTVNIDRDQARLYDLIWKRTL
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHH
ASQMSDAQLERTNVKIEADNHDELFTASGEVLLFEGFLKVYLEGHDDDEEEQEGMLPALK
HHHHHHHHEECCEEEEEECCCCCEEECCCCEEEEECHHHHEECCCCCCCHHHHCCCCHHH
VNEKLANNYITATERYSRPPARYTEASLVKKLEELGIGRPSTYAPTISTIINRNYVEKGT
HHHHHHCCCEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
LEGQERNYTQLTLQNSKVGEKLLKENTGSDKGKLVPTDIGTIVTDFLVKNFGNILDYNFT
CCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHCCHHCCCCEE
AKVEQDFDEIAEGNIDWAIMMQDFYNKFHPNVKEVEANAERESGERILGKDADGRQVSVR
EHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCHHHHHCCCCHHCCCEEECCCCCCCEEEEE
LGKFGPMAQIGEADDEDKKFASLMADQNIGNITLEEALNLFLLPKNLGEYKGEEVEVSNG
ECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEHHHCCCEEEECCCHHCCCCCEEEECCC
RYGPYVRHGSVFISLPRGEDPLNVTKERAKELIDEKALADAPIAVYKGEAVQKGVGRFGP
CCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCC
FIKWNGLFVNVSKKYNFDNLSQADVEELIEDKLQKNIDKVLHNWEEEGIVVEKARWGRSV
CEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEE
ILKGKIKIELSKDVDATKLTLAEVQEMIEKKAPAKKTAAKKTTAAKKTTTAKKAPAKKK
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
AKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGVDVENGFKPKYEVSPDK
CCCEEEEECCCHHHHHHHHCCCCEEEECCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCH
KALVTKLKSLSKNAETVWLASDEDREGEAISWHLAEELKLDTKKTKRIVFHEITKSAILK
HHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHH
AIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWRKIKGGLSAGRVQSVSVRLIVERER
HCCCCHHCCEEHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEEHHH
EIQSFNAVATYSIVAEFVNEAGKAFKAKLPKNFNTKKEAEDFLNKNIGSKYKVADLETKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCC
TKKSPTAPFTTSTLQQEAARKLYLPVGITMQLAQRLYEAGLITYMRTDSVNLSKEAMDAA
CCCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
QAEIIKSYGEEFSKPRIFATKSKGAQEAHEAIRPTDMSRHTVNIDRDQARLYDLIWKRTL
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHH
ASQMSDAQLERTNVKIEADNHDELFTASGEVLLFEGFLKVYLEGHDDDEEEQEGMLPALK
HHHHHHHHEECCEEEEEECCCCCEEECCCCEEEEECHHHHEECCCCCCCHHHHCCCCHHH
VNEKLANNYITATERYSRPPARYTEASLVKKLEELGIGRPSTYAPTISTIINRNYVEKGT
HHHHHHCCCEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
LEGQERNYTQLTLQNSKVGEKLLKENTGSDKGKLVPTDIGTIVTDFLVKNFGNILDYNFT
CCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHCCHHCCCCEE
AKVEQDFDEIAEGNIDWAIMMQDFYNKFHPNVKEVEANAERESGERILGKDADGRQVSVR
EHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCHHHHHCCCCHHCCCEEECCCCCCCEEEEE
LGKFGPMAQIGEADDEDKKFASLMADQNIGNITLEEALNLFLLPKNLGEYKGEEVEVSNG
ECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEHHHCCCEEEECCCHHCCCCCEEEECCC
RYGPYVRHGSVFISLPRGEDPLNVTKERAKELIDEKALADAPIAVYKGEAVQKGVGRFGP
CCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCC
FIKWNGLFVNVSKKYNFDNLSQADVEELIEDKLQKNIDKVLHNWEEEGIVVEKARWGRSV
CEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEE
ILKGKIKIELSKDVDATKLTLAEVQEMIEKKAPAKKTAAKKTTAAKKTTTAKKAPAKKK
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]