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The map label for this gene is murE [H]

Identifier: 146299566

GI number: 146299566

Start: 2086838

End: 2088301

Strand: Direct

Name: murE [H]

Synonym: Fjoh_1806

Alternate gene names: 146299566

Gene position: 2086838-2088301 (Clockwise)

Preceding gene: 146299565

Following gene: 146299567

Centisome position: 34.23

GC content: 34.49

Gene sequence:

>1464_bases
GTGAAAGTATTAAAAGACATATTATATAAAGTAACTATTGAATCTGTAACAGGTTCAACAGATATTGATATTCAGAAAAT
CGAATTTGATTCACGCAAAGTAGAATCAAATGATGTTTTTGTGGCAATTCGCGGTTCACTTTCTGATGGACACGATTATA
TTGAAAAAGCAATTCAGTTAGGTGCAAAAGCTATTATCTGCGACAAACTTCCTGAAAATATTCAGAAAGACGTTACTTAT
ATTCAGGTCAAAGATACCAATACAGCTTTGGCTTTTATGGCAGCTAATTATTTTGGAGATCCATCTGCAAAACTAAAATT
AGTCGGCGTAACAGGTACAAACGGAAAAACGACAATTGCATCATTATTGTTTCAGCTGTTTGAAAAAGCAGGCTTTAAAG
TCGGCTTATTATCAACAGTAAAAATTGTAGTTGATAAAACAGAATATCCGGCGACACACACAACACCAGATTCTTTAACC
ATAAATCATTATTTAAATGAAATGGCTGAAGCTGGAGTTACGCACTGTTTTATGGAAGTAAGTTCACACGGAATCCACCA
AAAACGTACTGAAGCTTTGCATTTTGTGGGCGGAATTTTCACGAATCTTTCGCATGATCACTTAGATTATCACCCAACGT
TTGCTGAATATAGAGACGTAAAAAAATCATTTTTTGATTCTCTGCCAAAAACTGCTTTTGTATTATCAAACATTGATGAT
AAAAATGGTTCAGTAATGCTGCAAAATACTGCTGCTAAAAAACTGACTTACGCTTTGAAATCTTATGCAGATTACAGAGC
TCAGATTTTAGAAAGCCAGTTGTCCGGATTATTACTGAAAGTTAATGATAATGAAGTTTGGGTAAAACTAATTGGGACTT
TCAACGCTTACAATGTTTTAGCTATTTATGGTACTGCAGTAGAGCTTGGAATTGATAGTTTAGAAGCGTTGCGTTTATTG
TCTGATTTAGAAAGTGTTTCGGGGCGTTTTCAGTATATCGTTTCAGATAACGGAATTACAGCTGTTGTAGATTATGCACA
TACGCCGGATGCATTAGAAAATGTTCTAAAGACGATAAATGATATCCGTACTAAAAACGAACAATTGATTACAGTTGTGG
GATGCGGCGGAAATCGTGATAAAACAAAAAGACCAATTATGGCAAAAATTGCGACAGATCTTAGTGATAAAGCAATTTTA
ACATCAGACAACCCAAGAAACGAAGATCCCGAAGTGATTTTGGATGAAATGGAACAAGGTGTTGAAGGTCATAATTATAA
AAAAGTACTGAGAATTACCGACAGAAAACAAGCTATTAAAACAGCTTGCCAATTAGCTCAGTCAAAAGATATCATTTTAA
TTGCCGGTAAAGGACACGAGACTTATCAGGAAATAAATGGGGTTCGCCATCATTTTGATGATATGGAGACAGTAAAAGAA
ATTTTAGAACAACTGAATAAATAA

Upstream 100 bases:

>100_bases
GTTTGAAAGTAAAAGTAAATGGAATGGGGAAAGTAAAAAATCAATCGATTCAAGCTGGAACCAGTATTAGCAAAAACACA
ACAATTGTATTAGAATTATC

Downstream 100 bases:

>100_bases
TAAAAGATTTTAAGTTCAAATACCACCACACAATTGGAATTTGGAATTTCAAAATTGGAATTTAAAAAATAGAGAAATAT
GCTGTACTATTTATTTGAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKVLKDILYKVTIESVTGSTDIDIQKIEFDSRKVESNDVFVAIRGSLSDGHDYIEKAIQLGAKAIICDKLPENIQKDVTY
IQVKDTNTALAFMAANYFGDPSAKLKLVGVTGTNGKTTIASLLFQLFEKAGFKVGLLSTVKIVVDKTEYPATHTTPDSLT
INHYLNEMAEAGVTHCFMEVSSHGIHQKRTEALHFVGGIFTNLSHDHLDYHPTFAEYRDVKKSFFDSLPKTAFVLSNIDD
KNGSVMLQNTAAKKLTYALKSYADYRAQILESQLSGLLLKVNDNEVWVKLIGTFNAYNVLAIYGTAVELGIDSLEALRLL
SDLESVSGRFQYIVSDNGITAVVDYAHTPDALENVLKTINDIRTKNEQLITVVGCGGNRDKTKRPIMAKIATDLSDKAIL
TSDNPRNEDPEVILDEMEQGVEGHNYKKVLRITDRKQAIKTACQLAQSKDIILIAGKGHETYQEINGVRHHFDDMETVKE
ILEQLNK

Sequences:

>Translated_487_residues
MKVLKDILYKVTIESVTGSTDIDIQKIEFDSRKVESNDVFVAIRGSLSDGHDYIEKAIQLGAKAIICDKLPENIQKDVTY
IQVKDTNTALAFMAANYFGDPSAKLKLVGVTGTNGKTTIASLLFQLFEKAGFKVGLLSTVKIVVDKTEYPATHTTPDSLT
INHYLNEMAEAGVTHCFMEVSSHGIHQKRTEALHFVGGIFTNLSHDHLDYHPTFAEYRDVKKSFFDSLPKTAFVLSNIDD
KNGSVMLQNTAAKKLTYALKSYADYRAQILESQLSGLLLKVNDNEVWVKLIGTFNAYNVLAIYGTAVELGIDSLEALRLL
SDLESVSGRFQYIVSDNGITAVVDYAHTPDALENVLKTINDIRTKNEQLITVVGCGGNRDKTKRPIMAKIATDLSDKAIL
TSDNPRNEDPEVILDEMEQGVEGHNYKKVLRITDRKQAIKTACQLAQSKDIILIAGKGHETYQEINGVRHHFDDMETVKE
ILEQLNK
>Mature_487_residues
MKVLKDILYKVTIESVTGSTDIDIQKIEFDSRKVESNDVFVAIRGSLSDGHDYIEKAIQLGAKAIICDKLPENIQKDVTY
IQVKDTNTALAFMAANYFGDPSAKLKLVGVTGTNGKTTIASLLFQLFEKAGFKVGLLSTVKIVVDKTEYPATHTTPDSLT
INHYLNEMAEAGVTHCFMEVSSHGIHQKRTEALHFVGGIFTNLSHDHLDYHPTFAEYRDVKKSFFDSLPKTAFVLSNIDD
KNGSVMLQNTAAKKLTYALKSYADYRAQILESQLSGLLLKVNDNEVWVKLIGTFNAYNVLAIYGTAVELGIDSLEALRLL
SDLESVSGRFQYIVSDNGITAVVDYAHTPDALENVLKTINDIRTKNEQLITVVGCGGNRDKTKRPIMAKIATDLSDKAIL
TSDNPRNEDPEVILDEMEQGVEGHNYKKVLRITDRKQAIKTACQLAQSKDIILIAGKGHETYQEINGVRHHFDDMETVKE
ILEQLNK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=481, Percent_Identity=35.1351351351351, Blast_Score=236, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54125; Mature: 54125

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLKDILYKVTIESVTGSTDIDIQKIEFDSRKVESNDVFVAIRGSLSDGHDYIEKAIQL
CCHHHHHHHHEEHHHCCCCCCCEEEEEECCCCEECCCCEEEEEECCCCCCHHHHHHHHHH
GAKAIICDKLPENIQKDVTYIQVKDTNTALAFMAANYFGDPSAKLKLVGVTGTNGKTTIA
CCCEEEHHHCHHHHHCCCEEEEEECCCCEEEEEEHHHCCCCCCEEEEEEEECCCCHHHHH
SLLFQLFEKAGFKVGLLSTVKIVVDKTEYPATHTTPDSLTINHYLNEMAEAGVTHCFMEV
HHHHHHHHHCCCEEEHHEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
SSHGIHQKRTEALHFVGGIFTNLSHDHLDYHPTFAEYRDVKKSFFDSLPKTAFVLSNIDD
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
KNGSVMLQNTAAKKLTYALKSYADYRAQILESQLSGLLLKVNDNEVWVKLIGTFNAYNVL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEECCCCEEEE
AIYGTAVELGIDSLEALRLLSDLESVSGRFQYIVSDNGITAVVDYAHTPDALENVLKTIN
EEECCHHHHCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHH
DIRTKNEQLITVVGCGGNRDKTKRPIMAKIATDLSDKAILTSDNPRNEDPEVILDEMEQG
HHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCC
VEGHNYKKVLRITDRKQAIKTACQLAQSKDIILIAGKGHETYQEINGVRHHFDDMETVKE
CCCCCCHHHEEEHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHH
ILEQLNK
HHHHHCC
>Mature Secondary Structure
MKVLKDILYKVTIESVTGSTDIDIQKIEFDSRKVESNDVFVAIRGSLSDGHDYIEKAIQL
CCHHHHHHHHEEHHHCCCCCCCEEEEEECCCCEECCCCEEEEEECCCCCCHHHHHHHHHH
GAKAIICDKLPENIQKDVTYIQVKDTNTALAFMAANYFGDPSAKLKLVGVTGTNGKTTIA
CCCEEEHHHCHHHHHCCCEEEEEECCCCEEEEEEHHHCCCCCCEEEEEEEECCCCHHHHH
SLLFQLFEKAGFKVGLLSTVKIVVDKTEYPATHTTPDSLTINHYLNEMAEAGVTHCFMEV
HHHHHHHHHCCCEEEHHEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
SSHGIHQKRTEALHFVGGIFTNLSHDHLDYHPTFAEYRDVKKSFFDSLPKTAFVLSNIDD
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
KNGSVMLQNTAAKKLTYALKSYADYRAQILESQLSGLLLKVNDNEVWVKLIGTFNAYNVL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEECCCCEEEE
AIYGTAVELGIDSLEALRLLSDLESVSGRFQYIVSDNGITAVVDYAHTPDALENVLKTIN
EEECCHHHHCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHH
DIRTKNEQLITVVGCGGNRDKTKRPIMAKIATDLSDKAILTSDNPRNEDPEVILDEMEQG
HHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCC
VEGHNYKKVLRITDRKQAIKTACQLAQSKDIILIAGKGHETYQEINGVRHHFDDMETVKE
CCCCCCHHHEEEHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHH
ILEQLNK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA