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The map label for this gene is pdhB [H]

Identifier: 146299235

GI number: 146299235

Start: 1688870

End: 1689847

Strand: Direct

Name: pdhB [H]

Synonym: Fjoh_1475

Alternate gene names: 146299235

Gene position: 1688870-1689847 (Clockwise)

Preceding gene: 146299230

Following gene: 146299236

Centisome position: 27.7

GC content: 38.96

Gene sequence:

>978_bases
ATGAGAACAATACAATTTAGAGAGGCCATTTGTGAAGCAATGAGCGAAGAAATGCGCCGCGATGAATCCATATATTTAAT
GGGAGAAGAGGTTGCAGAATACAACGGAGCTTACAAAGCTTCAAAAGGAATGCTTGCTGAGTTTGGTGAAAAGAGAGTGA
TCGATACTCCAATCGCTGAGCTTGGATTTTCAGGAATTGCAGTAGGTTCTGCAATGAATGGAAACCGCCCAATTGTAGAA
TATATGACATTCAACTTCTGTTTAGTTGGTATTGATCAAATTATAAATAACGCTGCTAAAATGCGTCAAATGACAGGAGG
ACAGTTTAATGTGCCTATTGTTTTCCGCGGACCAACGGCTTCTGCTGGTCAATTAGGAGCTACTCACTCACAAGCTTTAG
AAAACTGGTTTGCTAATACTCCAGGTCTTAAAGTTGTTGTTCCTTCAACTCCTTACGATGCAAAAGGACTTTTAAAATCA
GCAATTCGTGATAATGATCCGGTTATTTTCATGGAATCTGAGCAAATGTACGGAGACAAAGGTGAAGTGCCAGACGGAGA
ATACACAATTCCTCTAGGTGTTGCTGATGTTAAACGTGAAGGAACTGATGTAACTATCGTTTCTTTTGGAAAAATCATCA
AAGAAGCTTTTATCGCTGCTGATGAATTAGCTAAAGAAGGAATTTCATGTGAAATTATCGATTTAAGAACTGTTCGTCCA
ATGGATAAAGATGCGATCTTAAAATCGGTTAAAAAAACAAACCGTTTAGTAATTCTTGAAGAAGCTTGGCCGGTTGCCAG
CCTTTCTTCTGAAATTTCTTATATCGTTCAGGAGCAGGCATTCGATTTCCTTGATGCGCCAATTCAACGTATTACAACTG
CAGATACTCCTGCGCCGTATTCTCCAGTATTGCTTAAAGACTGGTTGCCAAATGCAGGTGATGTAGTAAAAGCAGTTAAA
AAAGTGTTATACAAATAG

Upstream 100 bases:

>100_bases
AGAGAAAGAACTTCATGCGATTTTAAATTCAATTTATGCATTTAAGTTATTTAAAATATTTATTTTTGCTCTTCAGAAAA
TTCAACATACAATAAAAAAT

Downstream 100 bases:

>100_bases
TATATATTTAAAATACTCACAAAACTTCATCATTCATAGTTAATTGATGAAGTTTTTTTTTGCCCAGACAATGAAAAGAA
CAATTTTACTCAGCCTATTT

Product: transketolase, central region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRTIQFREAICEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGMLAEFGEKRVIDTPIAELGFSGIAVGSAMNGNRPIVE
YMTFNFCLVGIDQIINNAAKMRQMTGGQFNVPIVFRGPTASAGQLGATHSQALENWFANTPGLKVVVPSTPYDAKGLLKS
AIRDNDPVIFMESEQMYGDKGEVPDGEYTIPLGVADVKREGTDVTIVSFGKIIKEAFIAADELAKEGISCEIIDLRTVRP
MDKDAILKSVKKTNRLVILEEAWPVASLSSEISYIVQEQAFDFLDAPIQRITTADTPAPYSPVLLKDWLPNAGDVVKAVK
KVLYK

Sequences:

>Translated_325_residues
MRTIQFREAICEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGMLAEFGEKRVIDTPIAELGFSGIAVGSAMNGNRPIVE
YMTFNFCLVGIDQIINNAAKMRQMTGGQFNVPIVFRGPTASAGQLGATHSQALENWFANTPGLKVVVPSTPYDAKGLLKS
AIRDNDPVIFMESEQMYGDKGEVPDGEYTIPLGVADVKREGTDVTIVSFGKIIKEAFIAADELAKEGISCEIIDLRTVRP
MDKDAILKSVKKTNRLVILEEAWPVASLSSEISYIVQEQAFDFLDAPIQRITTADTPAPYSPVLLKDWLPNAGDVVKAVK
KVLYK
>Mature_325_residues
MRTIQFREAICEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGMLAEFGEKRVIDTPIAELGFSGIAVGSAMNGNRPIVE
YMTFNFCLVGIDQIINNAAKMRQMTGGQFNVPIVFRGPTASAGQLGATHSQALENWFANTPGLKVVVPSTPYDAKGLLKS
AIRDNDPVIFMESEQMYGDKGEVPDGEYTIPLGVADVKREGTDVTIVSFGKIIKEAFIAADELAKEGISCEIIDLRTVRP
MDKDAILKSVKKTNRLVILEEAWPVASLSSEISYIVQEQAFDFLDAPIQRITTADTPAPYSPVLLKDWLPNAGDVVKAVK
KVLYK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=49.8461538461538, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=47.0769230769231, Blast_Score=311, Evalue=4e-85,
Organism=Homo sapiens, GI4557353, Length=321, Percent_Identity=38.9408099688474, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI34101272, Length=321, Percent_Identity=38.9408099688474, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1786622, Length=234, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=50.920245398773, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17506935, Length=318, Percent_Identity=39.937106918239, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=48.780487804878, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=47.7064220183486, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=47.7064220183486, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI160714828, Length=320, Percent_Identity=36.5625, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI160714832, Length=320, Percent_Identity=36.5625, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24650943, Length=92, Percent_Identity=51.0869565217391, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650945, Length=92, Percent_Identity=51.0869565217391, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35704; Mature: 35704

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIQFREAICEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGMLAEFGEKRVIDTPIAE
CCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCHHCCCCHHHHHCCCCCCCCCHHH
LGFSGIAVGSAMNGNRPIVEYMTFNFCLVGIDQIINNAAKMRQMTGGQFNVPIVFRGPTA
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEECCCC
SAGQLGATHSQALENWFANTPGLKVVVPSTPYDAKGLLKSAIRDNDPVIFMESEQMYGDK
CCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECHHHCCCC
GEVPDGEYTIPLGVADVKREGTDVTIVSFGKIIKEAFIAADELAKEGISCEIIDLRTVRP
CCCCCCCEEEEECHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
MDKDAILKSVKKTNRLVILEEAWPVASLSSEISYIVQEQAFDFLDAPIQRITTADTPAPY
CCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SPVLLKDWLPNAGDVVKAVKKVLYK
CCHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRTIQFREAICEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGMLAEFGEKRVIDTPIAE
CCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCHHCCCCHHHHHCCCCCCCCCHHH
LGFSGIAVGSAMNGNRPIVEYMTFNFCLVGIDQIINNAAKMRQMTGGQFNVPIVFRGPTA
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEECCCC
SAGQLGATHSQALENWFANTPGLKVVVPSTPYDAKGLLKSAIRDNDPVIFMESEQMYGDK
CCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECHHHCCCC
GEVPDGEYTIPLGVADVKREGTDVTIVSFGKIIKEAFIAADELAKEGISCEIIDLRTVRP
CCCCCCCEEEEECHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
MDKDAILKSVKKTNRLVILEEAWPVASLSSEISYIVQEQAFDFLDAPIQRITTADTPAPY
CCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SPVLLKDWLPNAGDVVKAVKKVLYK
CCHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]