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The map label for this gene is mscL

Identifier: 146299079

GI number: 146299079

Start: 1511089

End: 1511469

Strand: Direct

Name: mscL

Synonym: Fjoh_1319

Alternate gene names: 146299079

Gene position: 1511089-1511469 (Clockwise)

Preceding gene: 146299078

Following gene: 146299080

Centisome position: 24.78

GC content: 35.43

Gene sequence:

>381_bases
ATGGGATTTTTTTCAGAATTTAAGGAATTTGCAATGAAAGGCAACGTGGTTGATCTTGCTGTCGGTGTAATCATTGGAGC
TGCTTTTGGTAAAATCGTGAGCTCATTTATAGAAGATGTAATTACGCCGCTGTTATTGAAACCGGCTTTAGATGCTGCTA
ATTTATCAACTATTGAGCAGTTAACTGCTTTTGGAGGAGTAAAATACGGACTGTTTTTATCGGCTGTAATAAACTTTATA
ATTGTTGCATTTGTTTTATTTTTGATCATTAAAGCAATGAACCATGCTAAAAAGAAAGATGTTGCACCGCCACCGCCTCC
AGCCGGACCAACTCAGGAAGAATTGCTTACACAAATTAGAGATTTATTAAAAAATAAATAA

Upstream 100 bases:

>100_bases
CGCAGCGTGTAAAACGAGTATTTTTTAGATAGAGTACCGAACTTTCAGGAAAATTGCGTATTTTCGATATTCAATTATTA
TAAACAAAAACAGATCAGAT

Downstream 100 bases:

>100_bases
AAATATACCGCTGCACTAAGTCTAACTTAACCGCCTTCTAAAGAGGCGGTTTTTTTACAAATTGTTTAAAATACAACATT
TTGTTATTTTTTGTGAATCA

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MGFFSEFKEFAMKGNVVDLAVGVIIGAAFGKIVSSFIEDVITPLLLKPALDAANLSTIEQLTAFGGVKYGLFLSAVINFI
IVAFVLFLIIKAMNHAKKKDVAPPPPPAGPTQEELLTQIRDLLKNK

Sequences:

>Translated_126_residues
MGFFSEFKEFAMKGNVVDLAVGVIIGAAFGKIVSSFIEDVITPLLLKPALDAANLSTIEQLTAFGGVKYGLFLSAVINFI
IVAFVLFLIIKAMNHAKKKDVAPPPPPAGPTQEELLTQIRDLLKNK
>Mature_125_residues
GFFSEFKEFAMKGNVVDLAVGVIIGAAFGKIVSSFIEDVITPLLLKPALDAANLSTIEQLTAFGGVKYGLFLSAVINFII
VAFVLFLIIKAMNHAKKKDVAPPPPPAGPTQEELLTQIRDLLKNK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=133, Percent_Identity=50.3759398496241, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_FLAJ1 (A5FKC3)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193670.1
- ProteinModelPortal:   A5FKC3
- SMR:   A5FKC3
- STRING:   A5FKC3
- GeneID:   5092324
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1319
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   CLSK2537607
- BioCyc:   FJOH376686:FJOH_1319-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 13574; Mature: 13443

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e178bf8)-; HASH(0x1dc727b0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFFSEFKEFAMKGNVVDLAVGVIIGAAFGKIVSSFIEDVITPLLLKPALDAANLSTIEQ
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LTAFGGVKYGLFLSAVINFIIVAFVLFLIIKAMNHAKKKDVAPPPPPAGPTQEELLTQIR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DLLKNK
HHHCCH
>Mature Secondary Structure 
GFFSEFKEFAMKGNVVDLAVGVIIGAAFGKIVSSFIEDVITPLLLKPALDAANLSTIEQ
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LTAFGGVKYGLFLSAVINFIIVAFVLFLIIKAMNHAKKKDVAPPPPPAGPTQEELLTQIR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DLLKNK
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA