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The map label for this gene is nirE [H]

Identifier: 146283854

GI number: 146283854

Start: 3824062

End: 3824898

Strand: Direct

Name: nirE [H]

Synonym: PST_3537

Alternate gene names: 146283854

Gene position: 3824062-3824898 (Clockwise)

Preceding gene: 146283853

Following gene: 146283855

Centisome position: 83.72

GC content: 68.34

Gene sequence:

>837_bases
ATGGACCAGCCACTCACACTTCCCGCTTCGCTGAGCGCCGCGTTCGCCCCCGGCGAAGTCGCTCTGGTCGGCGCCGGCCC
GGGTGATCCCGGTCTGCTCACCCTGCGTGCCTGGAGCCTGCTGCAACAGGCCGATGCGGTGGTCTACGACCGGCTGGTCA
GCGATGCCCTGATGGACCTGCTGCCCGCCGCCTGCAGCCGCCACTATGTCGGCAAGACCAGCGGTTATCACAGCCTGCCG
CAACCGGAAATCAATCAGTTGCTCGCCGACCTGGCAAAGCAGAACAAGCGCGTGGTGCGGCTCAAGGGCGGCGACCCGTT
CATCTTCGGTCGCGGCGCCGAAGAGCTGGAGTTCCTTCTGCAGCGCGGCATCGACTGCCAGGTGGTGCCGGGCATCACCG
CCGCAGCCGGTTGCAGCGCCTATGCTGGCATCCCGCTGACCCATCGCGACCTGGCGCATTCCTGTCAGTTCATCACCGGC
CACCTGCAGACCAACGGCGAACTGCACCTGCCGTGGGACTCACTGGCCCAGGGCGGCCAGACCCTGGTGTTCTACATGGG
GCTGGCCAGCCTCGGCGAGATCTCGCGCAACCTGATCGCCGCCGGCCTGCCGGTGGATACCCCGGCCGCACTGATCGGCA
ACGGCACCCGGCCGGACCAGCAGGTGGTGCGCTGCACGCTACGGCAACTGCCGAACATGGCCGACGAGCAGCACCTCACC
CCACCGACTCTCACCGTAATCGGCAAGGTGGTGGGCCTGTTCGCCGAGCGCCAGGTCCGCCACCCGGCGCGCCTGCACGG
CGACCCGCTCGCCACCGCGGAGGCCTTATGCAACTGA

Upstream 100 bases:

>100_bases
CTGCCGACACCCGAGCGCATTCCCAGCATCGCCATTTGATCGAAGCGGCCGCCAAGCGCCGCCCGCACACCGTAGTCCGA
TGAGCATTCAGGAGCACCGC

Downstream 100 bases:

>100_bases
TACCCGCCCTGCTGCTGGCCGCCGCGCTGCCGGCCGCCACCTTCGTCGCCCTCGACATGGCCAGTGCTGCCACGCCGGAC
GACAGCCAGGCCCTGTATGA

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MDQPLTLPASLSAAFAPGEVALVGAGPGDPGLLTLRAWSLLQQADAVVYDRLVSDALMDLLPAACSRHYVGKTSGYHSLP
QPEINQLLADLAKQNKRVVRLKGGDPFIFGRGAEELEFLLQRGIDCQVVPGITAAAGCSAYAGIPLTHRDLAHSCQFITG
HLQTNGELHLPWDSLAQGGQTLVFYMGLASLGEISRNLIAAGLPVDTPAALIGNGTRPDQQVVRCTLRQLPNMADEQHLT
PPTLTVIGKVVGLFAERQVRHPARLHGDPLATAEALCN

Sequences:

>Translated_278_residues
MDQPLTLPASLSAAFAPGEVALVGAGPGDPGLLTLRAWSLLQQADAVVYDRLVSDALMDLLPAACSRHYVGKTSGYHSLP
QPEINQLLADLAKQNKRVVRLKGGDPFIFGRGAEELEFLLQRGIDCQVVPGITAAAGCSAYAGIPLTHRDLAHSCQFITG
HLQTNGELHLPWDSLAQGGQTLVFYMGLASLGEISRNLIAAGLPVDTPAALIGNGTRPDQQVVRCTLRQLPNMADEQHLT
PPTLTVIGKVVGLFAERQVRHPARLHGDPLATAEALCN
>Mature_278_residues
MDQPLTLPASLSAAFAPGEVALVGAGPGDPGLLTLRAWSLLQQADAVVYDRLVSDALMDLLPAACSRHYVGKTSGYHSLP
QPEINQLLADLAKQNKRVVRLKGGDPFIFGRGAEELEFLLQRGIDCQVVPGITAAAGCSAYAGIPLTHRDLAHSCQFITG
HLQTNGELHLPWDSLAQGGQTLVFYMGLASLGEISRNLIAAGLPVDTPAALIGNGTRPDQQVVRCTLRQLPNMADEQHLT
PPTLTVIGKVVGLFAERQVRHPARLHGDPLATAEALCN

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=236, Percent_Identity=59.3220338983051, Blast_Score=270, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6322922, Length=242, Percent_Identity=34.7107438016529, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 29538; Mature: 29538

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQPLTLPASLSAAFAPGEVALVGAGPGDPGLLTLRAWSLLQQADAVVYDRLVSDALMDL
CCCCCCCCHHCCCEECCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPAACSRHYVGKTSGYHSLPQPEINQLLADLAKQNKRVVRLKGGDPFIFGRGAEELEFLL
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHHHHH
QRGIDCQVVPGITAAAGCSAYAGIPLTHRDLAHSCQFITGHLQTNGELHLPWDSLAQGGQ
HCCCCEEECCCCCHHHCCCHHCCCCCCHHHHHHHHHHHEEEEECCCEEECCHHHHHCCCC
TLVFYMGLASLGEISRNLIAAGLPVDTPAALIGNGTRPDQQVVRCTLRQLPNMADEQHLT
EEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PPTLTVIGKVVGLFAERQVRHPARLHGDPLATAEALCN
CHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MDQPLTLPASLSAAFAPGEVALVGAGPGDPGLLTLRAWSLLQQADAVVYDRLVSDALMDL
CCCCCCCCHHCCCEECCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPAACSRHYVGKTSGYHSLPQPEINQLLADLAKQNKRVVRLKGGDPFIFGRGAEELEFLL
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHHHHH
QRGIDCQVVPGITAAAGCSAYAGIPLTHRDLAHSCQFITGHLQTNGELHLPWDSLAQGGQ
HCCCCEEECCCCCHHHCCCHHCCCCCCHHHHHHHHHHHEEEEECCCEEECCHHHHHCCCC
TLVFYMGLASLGEISRNLIAAGLPVDTPAALIGNGTRPDQQVVRCTLRQLPNMADEQHLT
EEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PPTLTVIGKVVGLFAERQVRHPARLHGDPLATAEALCN
CHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA