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The map label for this gene is nirJ [H]

Identifier: 146283853

GI number: 146283853

Start: 3822819

End: 3824000

Strand: Direct

Name: nirJ [H]

Synonym: PST_3536

Alternate gene names: 146283853

Gene position: 3822819-3824000 (Clockwise)

Preceding gene: 146283852

Following gene: 146283854

Centisome position: 83.7

GC content: 67.01

Gene sequence:

>1182_bases
ATGTTGAGAATCAGCCATTACCTGCGCGCCCTGGCCCAGCCGACCACGCCGGTGCTCGGCGCCCGCAGTGTCAGCGGCAA
GCGCCCACCCGTGGTGATCTGGAACCTGCTCAGGCGCTGCAACCTGACCTGCAGGCACTGCTATGCAACCTCCGCCGACA
GCGAATTCCGCGACGAGCTGGACACCGCCGAGGCGCTCCGCGTGATCGACGATCTGCACGAAGCCGGTGTGCGCGTGCTG
ATCCTCTCCGGCGGCGAGCCGCTGCTGCGCGGCGACATCTTCCAGCTGGCCGATTACGCTCGCGACAAGGGCTTCTTCGT
CGCCCTGTCGACCAACGGCACGCTGATCGACGAAGGCAACATCGCGCGCATCGCCGCGGCCCGGTTCGATTACGTCGGCA
TCAGCATCGATGGCCTGGAAGCGGTGCATGACGACTGGCGCCAGCTCAAGGGCAGCTTCGCCGCCTCCATGCACGCCATC
GACCTGTGTCGCCAGCACGACATCCGCGTCGGCCTGCGCACCACCCTGACACAGAACAACTACCCGCAGCTGCCCGCCCT
GCTGGCACTGATGCGTGAGCACGACGTACAGAAGTTCTACCTCTCGCACCTCAACTACAGCGGCCGTGGCAAGCGCAGCC
GCAAGGCCGACGCCCATCACCAGATGACCCGCGATGCCATGCGCCAGCTCTTCGAGCAGGCCTGGGACGACGTGCAGCAA
GGCCGCGAGACCGACTTCGTCAGCGGCAACAACGACGCCGACGCCATCCTCCTGCTGCAGTGGGTCGAGCAGCACCTGCC
CGAGCATCGCGAGCGCCTGGAAGGCATGTTGCGGGCCTGGGGCGGCAACGCCTCGGGCAGCGGCATCGCCAACATCGACA
ACATCGGCGACGTGCACCCGGACACCTACTGGTGGCAGCACACCGTGGGCAACGTGCGCCGCCAGCGTTTCAGCGACATC
TGGCTGCACGAGCCGGCCCCACTGCTGCAGGAGCTGCGCCAGCATCCGCGAGCGGTGAATGGCCGCTGTGCCGAATGCCG
CTGGCTGTCGATCTGCAACGGCAACACCCGTACCCGCGCCTGGGCGCAGGGCGACCTCTGGGCCGAGGACCCGGGTTGCT
ACCTCTCCGACGAGGAGATCGGTCTGCCGACACCCGAGCGCATTCCCAGCATCGCCATTTGA

Upstream 100 bases:

>100_bases
ATCAAGGCCAGGCCTATCCCACTCGGTGATCCTGCTGCTCAACCAAGGGGCGGCCAGCACGGCGCGCCCGCTGTGACACG
CTGCCGAAGCGGAGCCGCCC

Downstream 100 bases:

>100_bases
TCGAAGCGGCCGCCAAGCGCCGCCCGCACACCGTAGTCCGATGAGCATTCAGGAGCACCGCATGGACCAGCCACTCACAC
TTCCCGCTTCGCTGAGCGCC

Product: heme d1 biosynthesis protein NirJ

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLRISHYLRALAQPTTPVLGARSVSGKRPPVVIWNLLRRCNLTCRHCYATSADSEFRDELDTAEALRVIDDLHEAGVRVL
ILSGGEPLLRGDIFQLADYARDKGFFVALSTNGTLIDEGNIARIAAARFDYVGISIDGLEAVHDDWRQLKGSFAASMHAI
DLCRQHDIRVGLRTTLTQNNYPQLPALLALMREHDVQKFYLSHLNYSGRGKRSRKADAHHQMTRDAMRQLFEQAWDDVQQ
GRETDFVSGNNDADAILLLQWVEQHLPEHRERLEGMLRAWGGNASGSGIANIDNIGDVHPDTYWWQHTVGNVRRQRFSDI
WLHEPAPLLQELRQHPRAVNGRCAECRWLSICNGNTRTRAWAQGDLWAEDPGCYLSDEEIGLPTPERIPSIAI

Sequences:

>Translated_393_residues
MLRISHYLRALAQPTTPVLGARSVSGKRPPVVIWNLLRRCNLTCRHCYATSADSEFRDELDTAEALRVIDDLHEAGVRVL
ILSGGEPLLRGDIFQLADYARDKGFFVALSTNGTLIDEGNIARIAAARFDYVGISIDGLEAVHDDWRQLKGSFAASMHAI
DLCRQHDIRVGLRTTLTQNNYPQLPALLALMREHDVQKFYLSHLNYSGRGKRSRKADAHHQMTRDAMRQLFEQAWDDVQQ
GRETDFVSGNNDADAILLLQWVEQHLPEHRERLEGMLRAWGGNASGSGIANIDNIGDVHPDTYWWQHTVGNVRRQRFSDI
WLHEPAPLLQELRQHPRAVNGRCAECRWLSICNGNTRTRAWAQGDLWAEDPGCYLSDEEIGLPTPERIPSIAI
>Mature_393_residues
MLRISHYLRALAQPTTPVLGARSVSGKRPPVVIWNLLRRCNLTCRHCYATSADSEFRDELDTAEALRVIDDLHEAGVRVL
ILSGGEPLLRGDIFQLADYARDKGFFVALSTNGTLIDEGNIARIAAARFDYVGISIDGLEAVHDDWRQLKGSFAASMHAI
DLCRQHDIRVGLRTTLTQNNYPQLPALLALMREHDVQKFYLSHLNYSGRGKRSRKADAHHQMTRDAMRQLFEQAWDDVQQ
GRETDFVSGNNDADAILLLQWVEQHLPEHRERLEGMLRAWGGNASGSGIANIDNIGDVHPDTYWWQHTVGNVRRQRFSDI
WLHEPAPLLQELRQHPRAVNGRCAECRWLSICNGNTRTRAWAQGDLWAEDPGCYLSDEEIGLPTPERIPSIAI

Specific function: Unknown

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44480; Mature: 44480

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRISHYLRALAQPTTPVLGARSVSGKRPPVVIWNLLRRCNLTCRHCYATSADSEFRDEL
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHH
DTAEALRVIDDLHEAGVRVLILSGGEPLLRGDIFQLADYARDKGFFVALSTNGTLIDEGN
HHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
IARIAAARFDYVGISIDGLEAVHDDWRQLKGSFAASMHAIDLCRQHDIRVGLRTTLTQNN
EEEEEHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHEECCCC
YPQLPALLALMREHDVQKFYLSHLNYSGRGKRSRKADAHHQMTRDAMRQLFEQAWDDVQQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GRETDFVSGNNDADAILLLQWVEQHLPEHRERLEGMLRAWGGNASGSGIANIDNIGDVHP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DTYWWQHTVGNVRRQRFSDIWLHEPAPLLQELRQHPRAVNGRCAECRWLSICNGNTRTRA
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCCEEEEEEECCCCCCEE
WAQGDLWAEDPGCYLSDEEIGLPTPERIPSIAI
EECCCCCCCCCCCEECCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MLRISHYLRALAQPTTPVLGARSVSGKRPPVVIWNLLRRCNLTCRHCYATSADSEFRDEL
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHH
DTAEALRVIDDLHEAGVRVLILSGGEPLLRGDIFQLADYARDKGFFVALSTNGTLIDEGN
HHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
IARIAAARFDYVGISIDGLEAVHDDWRQLKGSFAASMHAIDLCRQHDIRVGLRTTLTQNN
EEEEEHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHEECCCC
YPQLPALLALMREHDVQKFYLSHLNYSGRGKRSRKADAHHQMTRDAMRQLFEQAWDDVQQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GRETDFVSGNNDADAILLLQWVEQHLPEHRERLEGMLRAWGGNASGSGIANIDNIGDVHP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DTYWWQHTVGNVRRQRFSDIWLHEPAPLLQELRQHPRAVNGRCAECRWLSICNGNTRTRA
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCCEEEEEEECCCCCCEE
WAQGDLWAEDPGCYLSDEEIGLPTPERIPSIAI
EECCCCCCCCCCCEECCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11111029 [H]