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The map label for this gene is purH

Identifier: 146283599

GI number: 146283599

Start: 3545382

End: 3546986

Strand: Direct

Name: purH

Synonym: PST_3279

Alternate gene names: 146283599

Gene position: 3545382-3546986 (Clockwise)

Preceding gene: 146283598

Following gene: 146283600

Centisome position: 77.62

GC content: 65.11

Gene sequence:

>1605_bases
ATGACCGACCAAACCACCCGCCTTCCCGTGCGCCGCGCGCTGATCAGCGTGTCCGACAAGACCGGCGTCGTCGACTTCGC
CCGTGAACTCGAAGCCCTTGGCGTCGAGATCCTGTCCACCGGTGGCACCTTCAAGCTGCTGCGCGAGAATGGCATCGCCG
CCATCGAGGTCGCCGACTACACCGGCTTCCCGGAAATGATGGATGGCCGGGTGAAGACCCTGCATCCGAAGATCCACGGC
GGCATCCTCGGCCGTCGTGATCTGGACGGTGCAGTCATGGCCGAGCACGGCATCGCGCCGATCGATCTGGTGGCGGTCAA
CCTCTACCCGTTCGCCGCCACCGTGGCCAAGCCCGGTTGCACCCTGCCGGACGCCATCGAGAATATCGATATCGGCGGCC
CGACCATGGTCCGCAGCGCGGCGAAGAACCACAAGGATGTCGCCATCGTGGTCAACGCCGAGGACTACGCGGCAGTGGTC
GACAACCTGAAGAACGGCGGGCTGACCTATGCCCAACGCTTCGACCTGGCGCTCAAGGCGTTCGAACACACCGCCGGCTA
TGACGGCATGATCGCCAACTACCTCGGCGGCATCGACCAGAGCACCGAGCAGCTCAGCACCGACAACCGCAGCCTGTTCC
CGCGCACCTACAACATGCAGTTCATCAAGGCGCAGGACATGCGCTACGGTGAGAACCCGCACCAGCAGGCCGCCTTCTAC
GTCGAGAAGCCGGACGAAGCCTGCGTCGCCACCGCCAGGCAGCTGCAGGGCAAGGAGCTGTCGTTCAACAACGTGGCCGA
CACCGACGCCGCGCTGGAGTGCGTGAAGAGCTTCACCAAGCCGGCCTGTGTGATCGTCAAGCATGCCAACCCCTGTGGCG
TCGCCGTCGTACCGGAAAACGAAGGCGGCATCCGCAAGGCCTATGACCTGGCCTACGCCACCGACAGCGAGTCGGCCTTC
GGCGGCATCATCGCCTTCAACCGCGAGCTGGACGGCGAAACCGCCCAGGCCATCGTCGAGCGTCAGTTCGTCGAGGTGAT
CATCGCGCCCAAGGTTTCCCAGGCAGCCCGCGATGTGGTCGCCAGCAAGGCCAACGTCCGCCTGCTCGAATGCGGCGAGT
GGCCGGCCGAGCGCAGTCCGGGCTGGGACTACAAGCGCGTCAACGGCGGCCTGCTGATCCAGAGCCGTGACATCGGCATG
ATCACCGAGGCGGACCTCAAGATCGTCACCCAGCGCGCACCGACCGAGCAGGAGATCCACGACCTGATCTTCGCCTGGAA
AGTGGCCAAGTTCGTCAAGTCCAACGCCATCGTCTACGCCAAGAACCGCCAGACCGTCGGTGTCGGCGCCGGCCAGATGA
GCCGCGTCAACTCCGCGCGCATCGCCGCGATCAAGGCCGAACACGCCGGTCTGCAGGTGCAGGGCGCGGTCATGGCTTCC
GATGCCTTCTTCCCGTTCCGCGACGGTATCGACAACGCAGCCAAGGCCGGCATCACCGCAGTGATCCAGCCGGGTGGCTC
GATGCGCGACAACGAAGTGATCGCCGCGGCCGACGAAGCCGGTATTGCCATGGTGTTCACCGGCATGCGCCACTTCCGCC
ACTAA

Upstream 100 bases:

>100_bases
CTCAAACAGTACGACCTGCTCTGAAGCGACAGCTGGCAGACTCGGTCGCCCGGTCCTGCCAGCTTCGCGGCCTCAAGCCT
GTAGCCTCTAGGAACCGTCA

Downstream 100 bases:

>100_bases
AATCGGCCGCACTCGGACAGGGATCTGCGGTGTTGCCCGACTCTTCCCCCATGCTCATTGCCAGAAGGCAACTCCGCTGG
GTGAAGAGTCGGGCGTCTTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTDQTTRLPVRRALISVSDKTGVVDFARELEALGVEILSTGGTFKLLRENGIAAIEVADYTGFPEMMDGRVKTLHPKIHG
GILGRRDLDGAVMAEHGIAPIDLVAVNLYPFAATVAKPGCTLPDAIENIDIGGPTMVRSAAKNHKDVAIVVNAEDYAAVV
DNLKNGGLTYAQRFDLALKAFEHTAGYDGMIANYLGGIDQSTEQLSTDNRSLFPRTYNMQFIKAQDMRYGENPHQQAAFY
VEKPDEACVATARQLQGKELSFNNVADTDAALECVKSFTKPACVIVKHANPCGVAVVPENEGGIRKAYDLAYATDSESAF
GGIIAFNRELDGETAQAIVERQFVEVIIAPKVSQAARDVVASKANVRLLECGEWPAERSPGWDYKRVNGGLLIQSRDIGM
ITEADLKIVTQRAPTEQEIHDLIFAWKVAKFVKSNAIVYAKNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGAVMAS
DAFFPFRDGIDNAAKAGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH

Sequences:

>Translated_534_residues
MTDQTTRLPVRRALISVSDKTGVVDFARELEALGVEILSTGGTFKLLRENGIAAIEVADYTGFPEMMDGRVKTLHPKIHG
GILGRRDLDGAVMAEHGIAPIDLVAVNLYPFAATVAKPGCTLPDAIENIDIGGPTMVRSAAKNHKDVAIVVNAEDYAAVV
DNLKNGGLTYAQRFDLALKAFEHTAGYDGMIANYLGGIDQSTEQLSTDNRSLFPRTYNMQFIKAQDMRYGENPHQQAAFY
VEKPDEACVATARQLQGKELSFNNVADTDAALECVKSFTKPACVIVKHANPCGVAVVPENEGGIRKAYDLAYATDSESAF
GGIIAFNRELDGETAQAIVERQFVEVIIAPKVSQAARDVVASKANVRLLECGEWPAERSPGWDYKRVNGGLLIQSRDIGM
ITEADLKIVTQRAPTEQEIHDLIFAWKVAKFVKSNAIVYAKNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGAVMAS
DAFFPFRDGIDNAAKAGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH
>Mature_533_residues
TDQTTRLPVRRALISVSDKTGVVDFARELEALGVEILSTGGTFKLLRENGIAAIEVADYTGFPEMMDGRVKTLHPKIHGG
ILGRRDLDGAVMAEHGIAPIDLVAVNLYPFAATVAKPGCTLPDAIENIDIGGPTMVRSAAKNHKDVAIVVNAEDYAAVVD
NLKNGGLTYAQRFDLALKAFEHTAGYDGMIANYLGGIDQSTEQLSTDNRSLFPRTYNMQFIKAQDMRYGENPHQQAAFYV
EKPDEACVATARQLQGKELSFNNVADTDAALECVKSFTKPACVIVKHANPCGVAVVPENEGGIRKAYDLAYATDSESAFG
GIIAFNRELDGETAQAIVERQFVEVIIAPKVSQAARDVVASKANVRLLECGEWPAERSPGWDYKRVNGGLLIQSRDIGMI
TEADLKIVTQRAPTEQEIHDLIFAWKVAKFVKSNAIVYAKNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGAVMASD
AFFPFRDGIDNAAKAGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=516, Percent_Identity=36.8217054263566, Blast_Score=270, Evalue=4e-72,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=68.7382297551789, Blast_Score=744, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=524, Percent_Identity=33.7786259541985, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=357, Percent_Identity=27.1708683473389, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=63, Percent_Identity=50.7936507936508, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=498, Percent_Identity=35.9437751004016, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6323056, Length=495, Percent_Identity=34.7474747474747, Blast_Score=246, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24649832, Length=506, Percent_Identity=36.9565217391304, Blast_Score=261, Evalue=1e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PSEU5 (A4VPK9)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173752.1
- STRING:   A4VPK9
- GeneID:   5096240
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3279
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   A4VPK9
- ProtClustDB:   PRK00881
- BioCyc:   PSTU379731:PST_3279-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57627; Mature: 57496

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQTTRLPVRRALISVSDKTGVVDFARELEALGVEILSTGGTFKLLRENGIAAIEVADY
CCCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHCEEEEECCCEEEEEECCCEEEEEEECC
TGFPEMMDGRVKTLHPKIHGGILGRRDLDGAVMAEHGIAPIDLVAVNLYPFAATVAKPGC
CCCHHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEEHHHHHHCCCCC
TLPDAIENIDIGGPTMVRSAAKNHKDVAIVVNAEDYAAVVDNLKNGGLTYAQRFDLALKA
CCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHH
FEHTAGYDGMIANYLGGIDQSTEQLSTDNRSLFPRTYNMQFIKAQDMRYGENPHQQAAFY
HHHCCCCCCHHHHHHCCCCCCHHHHCCCCCEECCEECCEEEEEECCCCCCCCCCCCEEEE
VEKPDEACVATARQLQGKELSFNNVADTDAALECVKSFTKPACVIVKHANPCGVAVVPEN
EECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECC
EGGIRKAYDLAYATDSESAFGGIIAFNRELDGETAQAIVERQFVEVIIAPKVSQAARDVV
CCCCEEHEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHH
ASKANVRLLECGEWPAERSPGWDYKRVNGGLLIQSRDIGMITEADLKIVTQRAPTEQEIH
HCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEEECCEEEEECCCCCHHHHH
DLIFAWKVAKFVKSNAIVYAKNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGAVMAS
HHHHHHHHHHHHHCCCEEEEECCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEEEEEEC
DAFFPFRDGIDNAAKAGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH
CCCCCHHCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure 
TDQTTRLPVRRALISVSDKTGVVDFARELEALGVEILSTGGTFKLLRENGIAAIEVADY
CCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHCEEEEECCCEEEEEECCCEEEEEEECC
TGFPEMMDGRVKTLHPKIHGGILGRRDLDGAVMAEHGIAPIDLVAVNLYPFAATVAKPGC
CCCHHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEEHHHHHHCCCCC
TLPDAIENIDIGGPTMVRSAAKNHKDVAIVVNAEDYAAVVDNLKNGGLTYAQRFDLALKA
CCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHH
FEHTAGYDGMIANYLGGIDQSTEQLSTDNRSLFPRTYNMQFIKAQDMRYGENPHQQAAFY
HHHCCCCCCHHHHHHCCCCCCHHHHCCCCCEECCEECCEEEEEECCCCCCCCCCCCEEEE
VEKPDEACVATARQLQGKELSFNNVADTDAALECVKSFTKPACVIVKHANPCGVAVVPEN
EECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECC
EGGIRKAYDLAYATDSESAFGGIIAFNRELDGETAQAIVERQFVEVIIAPKVSQAARDVV
CCCCEEHEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHH
ASKANVRLLECGEWPAERSPGWDYKRVNGGLLIQSRDIGMITEADLKIVTQRAPTEQEIH
HCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEEECCEEEEECCCCCHHHHH
DLIFAWKVAKFVKSNAIVYAKNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGAVMAS
HHHHHHHHHHHHHCCCEEEEECCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEEEEEEC
DAFFPFRDGIDNAAKAGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH
CCCCCHHCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA