Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 146283600

GI number: 146283600

Start: 3547186

End: 3548478

Strand: Direct

Name: purD [H]

Synonym: PST_3280

Alternate gene names: 146283600

Gene position: 3547186-3548478 (Clockwise)

Preceding gene: 146283599

Following gene: 146283601

Centisome position: 77.66

GC content: 65.97

Gene sequence:

>1293_bases
ATGAACGTACTGATCATCGGTAGCGGCGGCCGCGAGCATGCCCTGGCCTGGAAGGTCGCGCAGGACCCACGCGTTGCCAA
AGTATACGTAGCCCCCGGCAACGCAGGCACCGCCACTGAAGCCAAGTGCGAGAACGTCGCCATCGACGTGCTGGCCATCG
AGCAACTGGCGGACTTTGCCGCGGCCAACGTGCAGCTGACCATCGTCGGACCGGAAGCGCCGCTGGTCAAAGGCGTGGTC
GATCTGTTCCGCTCGCGCGGGCTGGACATCTTCGGCCCCACCGCTGCCGCCGCGCAGCTGGAAGGCTCTAAGGCCTTCAC
CAAGGATTTCCTGGCGCGTCACGCCATCCCGACCGCCGACTATCAGAACTTCACCGAAGTCGAACCGGCGCTGGCCTATC
TGCGCGAAAAGGGCGCACCGATCGTTATCAAGGCCGACGGCCTGGCGGCCGGCAAGGGCGTGATCGTCGCCATGACCCTG
GCTGAAGCCGAGGACGCCGTGCGTGACATGCTCTCCGGCAATGCCTTCGGCGACGCCGGGGCGCGGGTGGTGATCGAGGA
GTTCCTCGATGGCGAAGAGGCCAGCTTCATCGTCATGGTCGACGGCGAGCACGTGCTGCCGATGGCCACCAGCCAGGACC
ACAAGCGCGTCGGCGACGGCGACAGCGGCCCGAACACCGGCGGCATGGGCGCCTATTCGCCGGCTCCGGTGGTCACTGCC
GAGGTGCACCAGCGCGTAATGGACGAGGTGATCTACCCGACCGTGCGCGGCATGGCCGCCGAAGGCAACGTCTACACCGG
CTTCCTTTATGCCGGGCTGATGATCGACAAGGCCGGCAAGCCTAAGGTCATCGAGTTCAACTGCCGTTTCGGCGACCCGG
AAACCCAGCCAATCATGTGCCGCCTGGAGTCCAGCCTGGTGCTGCTGGTCGAGGCCGCGCTGGCCAAGGCGCTGAACAAG
GTCGAGGCGACCTGGGACCCGCGACCGACCGTCGGTGTGGTGCTGGCTGCCGGCGGCTACCCGGGCGACTATGCCAAGGG
CGACGTGATCGAGGGACTGGACGAAGCAGCCAAGCTGGATGGCAAGGTGTTCCACGCGGGCACCGCTTTGAATGCCGAGG
GTCAGGTAGTCACTGCCGGCGGCCGCGTACTCTGTGCCACCGCTATCGGCCGTAGCGTGTCCGAGGCACAGCAGCAGGCC
TACCGCCTGACGGAAAAGATCCGCTGGAATGGCTGTTTCTACCGCAAGGACATCGGCTACCGCGCCATCACCCGCGAGCG
TGGCGAGGGTTAA

Upstream 100 bases:

>100_bases
GCATCTACCAGCCCGATCGCGACCTCGGTATTCGTAGGGTGGATAACGCTTTGCTTATCCACCAAGCGGGCTACCGGCCC
GCCTCCATTGGGAGAGAGAA

Downstream 100 bases:

>100_bases
GCTCAGCCTGTCATCAGGAATGCGTGCGGCGCCAGGTTTTATCGCCGTCGCACGCATTACAGGCCGGCTGCGGCATGGCT
ATAATCCGCCGTCACACTCA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVLIIGSGGREHALAWKVAQDPRVAKVYVAPGNAGTATEAKCENVAIDVLAIEQLADFAAANVQLTIVGPEAPLVKGVV
DLFRSRGLDIFGPTAAAAQLEGSKAFTKDFLARHAIPTADYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDEVIYPTVRGMAAEGNVYTGFLYAGLMIDKAGKPKVIEFNCRFGDPETQPIMCRLESSLVLLVEAALAKALNK
VEATWDPRPTVGVVLAAGGYPGDYAKGDVIEGLDEAAKLDGKVFHAGTALNAEGQVVTAGGRVLCATAIGRSVSEAQQQA
YRLTEKIRWNGCFYRKDIGYRAITRERGEG

Sequences:

>Translated_430_residues
MNVLIIGSGGREHALAWKVAQDPRVAKVYVAPGNAGTATEAKCENVAIDVLAIEQLADFAAANVQLTIVGPEAPLVKGVV
DLFRSRGLDIFGPTAAAAQLEGSKAFTKDFLARHAIPTADYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDEVIYPTVRGMAAEGNVYTGFLYAGLMIDKAGKPKVIEFNCRFGDPETQPIMCRLESSLVLLVEAALAKALNK
VEATWDPRPTVGVVLAAGGYPGDYAKGDVIEGLDEAAKLDGKVFHAGTALNAEGQVVTAGGRVLCATAIGRSVSEAQQQA
YRLTEKIRWNGCFYRKDIGYRAITRERGEG
>Mature_430_residues
MNVLIIGSGGREHALAWKVAQDPRVAKVYVAPGNAGTATEAKCENVAIDVLAIEQLADFAAANVQLTIVGPEAPLVKGVV
DLFRSRGLDIFGPTAAAAQLEGSKAFTKDFLARHAIPTADYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDEVIYPTVRGMAAEGNVYTGFLYAGLMIDKAGKPKVIEFNCRFGDPETQPIMCRLESSLVLLVEAALAKALNK
VEATWDPRPTVGVVLAAGGYPGDYAKGDVIEGLDEAAKLDGKVFHAGTALNAEGQVVTAGGRVLCATAIGRSVSEAQQQA
YRLTEKIRWNGCFYRKDIGYRAITRERGEG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=51.6509433962264, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=51.6509433962264, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=51.6509433962264, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=51.6509433962264, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=74.7663551401869, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=43.0913348946136, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=41.8181818181818, Blast_Score=341, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=45.6876456876457, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=45.6876456876457, Blast_Score=341, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45423; Mature: 45423

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGGREHALAWKVAQDPRVAKVYVAPGNAGTATEAKCENVAIDVLAIEQLADFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEHEEHHHHHHHH
AANVQLTIVGPEAPLVKGVVDLFRSRGLDIFGPTAAAAQLEGSKAFTKDFLARHAIPTAD
HCCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCHHHHHCCCCHHHHHHHHHHHCCCCCC
YQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLSGNAFGDAG
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
ARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
HHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
EVHQRVMDEVIYPTVRGMAAEGNVYTGFLYAGLMIDKAGKPKVIEFNCRFGDPETQPIMC
HHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEE
RLESSLVLLVEAALAKALNKVEATWDPRPTVGVVLAAGGYPGDYAKGDVIEGLDEAAKLD
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCC
GKVFHAGTALNAEGQVVTAGGRVLCATAIGRSVSEAQQQAYRLTEKIRWNGCFYRKDIGY
CEEEECCEEECCCCCEEECCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
RAITRERGEG
EEECCCCCCC
>Mature Secondary Structure
MNVLIIGSGGREHALAWKVAQDPRVAKVYVAPGNAGTATEAKCENVAIDVLAIEQLADFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEHEEHHHHHHHH
AANVQLTIVGPEAPLVKGVVDLFRSRGLDIFGPTAAAAQLEGSKAFTKDFLARHAIPTAD
HCCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCHHHHHCCCCHHHHHHHHHHHCCCCCC
YQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLSGNAFGDAG
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
ARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
HHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
EVHQRVMDEVIYPTVRGMAAEGNVYTGFLYAGLMIDKAGKPKVIEFNCRFGDPETQPIMC
HHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEE
RLESSLVLLVEAALAKALNKVEATWDPRPTVGVVLAAGGYPGDYAKGDVIEGLDEAAKLD
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCC
GKVFHAGTALNAEGQVVTAGGRVLCATAIGRSVSEAQQQAYRLTEKIRWNGCFYRKDIGY
CEEEECCEEECCCCCEEECCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
RAITRERGEG
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]