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The map label for this gene is menB [C]

Identifier: 146283540

GI number: 146283540

Start: 3471146

End: 3471943

Strand: Direct

Name: menB [C]

Synonym: PST_3215

Alternate gene names: 146283540

Gene position: 3471146-3471943 (Clockwise)

Preceding gene: 146283539

Following gene: 146283541

Centisome position: 76.0

GC content: 67.54

Gene sequence:

>798_bases
ATGACCGATTTCCAGACCATCGAACTGAACAAAGACGCGCGCGGCGTGGCCACCCTCTGGCTCAACCGCGCGGACAAGAA
CAATGCCTTCGACGCCCAGGTGATCGGCGAACTGAACGCAGCGCTGGCCCAGGTGCAGGACGATGCGGGCATCCGCTTCC
TCATCCTGCGCGGGCGCGGCAAGCACTTCTCCGCTGGCGCGGATCTGGGCTGGATGCGCGAGTCCGCCAAACTCGACTAC
CAGGCGAACCTGGCTGACGCCCATGAACTCGGCGAGCTGATGCAGCGCCTTTATCAGCTGCCGCAGCCGACCCTGGCCGT
GGTACAGGGCGCGGCCTTTGGCGGCGCGCTGGGGCTGATCGCCTGCTGCGACATGGCCATCGGCGCCCGCGACGCACTGT
TCTGCCTGTCGGAAGTTCGCATCGGCCTGGCGCCGGCGGTGATCAGCCCGTACGTGGTCAAGGCCATCGGCGAGCGCGCC
ACCCGCCGCTACGCGCTGACCGCCGAACGCTTCGACGGCACACGTGCCCGCGAACTGGGCCTGCTCGCAGATACCTACCC
GGCCAGCGAGCTGGACGCCGCGCTGGAGCAATGGGTCGCCAACCTGCTGCTCAACAGCCCGCAGGCGCTCAAGGCCTGCA
AGGACCTGCTGCTGGAAGTGGGCGACGGCGATTTCAGCGCCGACCTGCGCCAGACCACCGAACAGGCCATCGCGCGCCTG
CGCACCAGCCCGGAAGGTCAGGAAGGCCTGAGCGCCTTTCTGGAAAAACGCACGCCCGCCTGGCAGGAGAAGAAGTGA

Upstream 100 bases:

>100_bases
TGCGCCGATCGAGCCGACCCGCTTCGGCGTGTTCCGCATGTAATCCGTGCCGCCGGCAGCCGAGCGCGCCGGCGCCTGCA
GTCGCATCGAGGATGCCGAG

Downstream 100 bases:

>100_bases
TGAATCGCCATATCGATACCCTGCTGGTCGCCAACCGCGGCGAGATCGCCTGCCGCGTGATGCGCACCGCCAAGGCCATG
GGCCTGACCACCGTTGCGGT

Product: gamma-carboxygeranoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTDFQTIELNKDARGVATLWLNRADKNNAFDAQVIGELNAALAQVQDDAGIRFLILRGRGKHFSAGADLGWMRESAKLDY
QANLADAHELGELMQRLYQLPQPTLAVVQGAAFGGALGLIACCDMAIGARDALFCLSEVRIGLAPAVISPYVVKAIGERA
TRRYALTAERFDGTRARELGLLADTYPASELDAALEQWVANLLLNSPQALKACKDLLLEVGDGDFSADLRQTTEQAIARL
RTSPEGQEGLSAFLEKRTPAWQEKK

Sequences:

>Translated_265_residues
MTDFQTIELNKDARGVATLWLNRADKNNAFDAQVIGELNAALAQVQDDAGIRFLILRGRGKHFSAGADLGWMRESAKLDY
QANLADAHELGELMQRLYQLPQPTLAVVQGAAFGGALGLIACCDMAIGARDALFCLSEVRIGLAPAVISPYVVKAIGERA
TRRYALTAERFDGTRARELGLLADTYPASELDAALEQWVANLLLNSPQALKACKDLLLEVGDGDFSADLRQTTEQAIARL
RTSPEGQEGLSAFLEKRTPAWQEKK
>Mature_264_residues
TDFQTIELNKDARGVATLWLNRADKNNAFDAQVIGELNAALAQVQDDAGIRFLILRGRGKHFSAGADLGWMRESAKLDYQ
ANLADAHELGELMQRLYQLPQPTLAVVQGAAFGGALGLIACCDMAIGARDALFCLSEVRIGLAPAVISPYVVKAIGERAT
RRYALTAERFDGTRARELGLLADTYPASELDAALEQWVANLLLNSPQALKACKDLLLEVGDGDFSADLRQTTEQAIARLR
TSPEGQEGLSAFLEKRTPAWQEKK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=24.4186046511628, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI194097323, Length=268, Percent_Identity=26.1194029850746, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=27.6679841897233, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI213417737, Length=213, Percent_Identity=23.4741784037559, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI157694516, Length=213, Percent_Identity=23.4741784037559, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=27.0676691729323, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1788597, Length=268, Percent_Identity=29.1044776119403, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=30.9236947791165, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=25.2873563218391, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=29.5019157088123, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=29.3436293436293, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=26.9911504424779, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=25.1908396946565, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=24.2307692307692, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=203, Percent_Identity=26.6009852216749, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=26.7326732673267, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=23.1939163498099, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920382, Length=234, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=26.4367816091954, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=26.4367816091954, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28832; Mature: 28701

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFQTIELNKDARGVATLWLNRADKNNAFDAQVIGELNAALAQVQDDAGIRFLILRGRG
CCCCEEEEECCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
KHFSAGADLGWMRESAKLDYQANLADAHELGELMQRLYQLPQPTLAVVQGAAFGGALGLI
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
ACCDMAIGARDALFCLSEVRIGLAPAVISPYVVKAIGERATRRYALTAERFDGTRARELG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCHHHHHC
LLADTYPASELDAALEQWVANLLLNSPQALKACKDLLLEVGDGDFSADLRQTTEQAIARL
CHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RTSPEGQEGLSAFLEKRTPAWQEKK
HCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDFQTIELNKDARGVATLWLNRADKNNAFDAQVIGELNAALAQVQDDAGIRFLILRGRG
CCCEEEEECCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
KHFSAGADLGWMRESAKLDYQANLADAHELGELMQRLYQLPQPTLAVVQGAAFGGALGLI
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
ACCDMAIGARDALFCLSEVRIGLAPAVISPYVVKAIGERATRRYALTAERFDGTRARELG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCHHHHHC
LLADTYPASELDAALEQWVANLLLNSPQALKACKDLLLEVGDGDFSADLRQTTEQAIARL
CHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RTSPEGQEGLSAFLEKRTPAWQEKK
HCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA