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The map label for this gene is rutR [H]

Identifier: 146283428

GI number: 146283428

Start: 3338808

End: 3339428

Strand: Direct

Name: rutR [H]

Synonym: PST_3101

Alternate gene names: 146283428

Gene position: 3338808-3339428 (Clockwise)

Preceding gene: 146283426

Following gene: 146283429

Centisome position: 73.1

GC content: 64.73

Gene sequence:

>621_bases
ATGTCCAGCATTCGAGAGCGCAACCGAGACGTGATCCTGCGAGCCGCCAGCGAGGAATTCGCGGAAAAGGGCTTCGCCGC
CACCAAGACCAGCGACATCGCCGCCCGCGCCGGGCTGCCGAAGCCCAACGTCTACTACTACTTCAAGTCCAAGGAAAACC
TCTATCGCGAGGTGCTGGAGAGCATCGTCGAACCGCTGCTCGAAGCCTCTGCACCGTTCAATCAGCCAGGCCATCCGGCC
GAGGTGCTGCGCGCCTACATCCGCACCAAGATCCGCATCTCCCGCGATCACGCCTATGCCTCCAAGGTGTTCGCCAGCGA
GATCATGCATGGCGCCCCGCACCTGTCACCGGAGCGCACCGCCCAGCTCAACGCCCAGGCCGCCCACAACATCGCCTGCA
TTCAGGGCTGGATCGACCAAGGTCTGATGGCCCCCATCGACCCCAGCCATCTGCTCTTCAGCATCTGGGCCGCGACCCAG
ACCTATGCCGACTTCGACTGGCAGATCAGCACCGTCACCGGCAAGGCCCGGCTCGACGAGGCGGACTACGATGCCGCTGC
CGACACCATCATCCGCCTGGTCCTCAGGGGCTGCGAAATCGCCGAGCCGACGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGAACCGACGGATGGCGGCTCGGTTGAGGCCGCAAAGAAGCGAGTGCTAGCATGCCGAACCGCCGCCAGGCCGGGCGG
CGCCAGCCAAACAGGTCCGC

Downstream 100 bases:

>100_bases
GACTGCGCACGGCAGCGCCCGCTGCTGGCGATGCCGTGATCGGGTGAGCGGAGGGCGAAATTCGTTGCAGCGCTTGTCCT
ACCTAGGACCGCGCCCGTCG

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSSIRERNRDVILRAASEEFAEKGFAATKTSDIAARAGLPKPNVYYYFKSKENLYREVLESIVEPLLEASAPFNQPGHPA
EVLRAYIRTKIRISRDHAYASKVFASEIMHGAPHLSPERTAQLNAQAAHNIACIQGWIDQGLMAPIDPSHLLFSIWAATQ
TYADFDWQISTVTGKARLDEADYDAAADTIIRLVLRGCEIAEPTAA

Sequences:

>Translated_206_residues
MSSIRERNRDVILRAASEEFAEKGFAATKTSDIAARAGLPKPNVYYYFKSKENLYREVLESIVEPLLEASAPFNQPGHPA
EVLRAYIRTKIRISRDHAYASKVFASEIMHGAPHLSPERTAQLNAQAAHNIACIQGWIDQGLMAPIDPSHLLFSIWAATQ
TYADFDWQISTVTGKARLDEADYDAAADTIIRLVLRGCEIAEPTAA
>Mature_205_residues
SSIRERNRDVILRAASEEFAEKGFAATKTSDIAARAGLPKPNVYYYFKSKENLYREVLESIVEPLLEASAPFNQPGHPAE
VLRAYIRTKIRISRDHAYASKVFASEIMHGAPHLSPERTAQLNAQAAHNIACIQGWIDQGLMAPIDPSHLLFSIWAATQT
YADFDWQISTVTGKARLDEADYDAAADTIIRLVLRGCEIAEPTAA

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=191, Percent_Identity=40.3141361256544, Blast_Score=151, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22846; Mature: 22715

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIRERNRDVILRAASEEFAEKGFAATKTSDIAARAGLPKPNVYYYFKSKENLYREVLE
CCCHHHCCCCEEEEEHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SIVEPLLEASAPFNQPGHPAEVLRAYIRTKIRISRDHAYASKVFASEIMHGAPHLSPERT
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AQLNAQAAHNIACIQGWIDQGLMAPIDPSHLLFSIWAATQTYADFDWQISTVTGKARLDE
HHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHCCC
ADYDAAADTIIRLVLRGCEIAEPTAA
CCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSIRERNRDVILRAASEEFAEKGFAATKTSDIAARAGLPKPNVYYYFKSKENLYREVLE
CCHHHCCCCEEEEEHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SIVEPLLEASAPFNQPGHPAEVLRAYIRTKIRISRDHAYASKVFASEIMHGAPHLSPERT
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AQLNAQAAHNIACIQGWIDQGLMAPIDPSHLLFSIWAATQTYADFDWQISTVTGKARLDE
HHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHCCC
ADYDAAADTIIRLVLRGCEIAEPTAA
CCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]