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The map label for this gene is trpA

Identifier: 146283231

GI number: 146283231

Start: 3118464

End: 3119273

Strand: Direct

Name: trpA

Synonym: PST_2896

Alternate gene names: 146283231

Gene position: 3118464-3119273 (Clockwise)

Preceding gene: 146283230

Following gene: 146283232

Centisome position: 68.28

GC content: 67.28

Gene sequence:

>810_bases
ATGAGCCGCCTGCTGACGCGCTTCGACGAGCTGAAACAACAGAACCGCGCCGCACTGGTGACCTTCATCACCGCCGGCGA
TCCGGACTACGCCACCTCGCTGCAGATCCTCAAGGGCCTGCCGGCCGCGGGTGCCGACGTCATCGAACTGGGCATGCCCT
TCACCGACCCGATGGCCGACGGCCCGGCGATTCAGCTGGCCAACATCCGCGCCCTGGCCGCCAAGCAGGACCTGGCCAAG
ACGCTGCAGATGGTTCGTGAATTCCGCCAGGACGACCAGACCACCCCGATCGTGCTGATGGGCTACTTCAACCCCATCCA
CAAGATGGGCGTGGAGCGCTTCATCGCCGAGGCGGTGGAAGCCGGCGTCGACGGCCTGATCGTGGTCGACCTGCCGCCGG
AGCACAACGAAGACCTCTGCGACCCGGCCCAGGCCGCGGGCATCGACTTCATCCGCCTGACCACCCCGACCACCGATGAC
AAGCGCCTGCCGGTGGTACTCAACGGCAGCTCCGGCTTCGTCTATTACGTCTCGGTGGCCGGCGTGACCGGTGCCGGTTC
GGCGACGCTCGAGCATGTCGAGGAAGCCGTGGCGCGCCTCAAGCGTCATACCGAGCTGCCGGTGTGCGTCGGCTTCGGCA
TCCGCACACCGGAACAGGCCGCCGCCATCGCCCGGCTGACCGAAGGGGTGGTAGTGGGCTCGGCGCTGATCGATCAGATC
GCCAATGCCAAGAGCAACGCACAGGCCGTCGAAGGCGTGCTGGAACTGTGCCGCCAGATCAGTACCGGCGTGCGCAGCGC
CCGCGCCTGA

Upstream 100 bases:

>100_bases
GTCTGCCGAAGGATCACCTGATGGTGGTCAACCTCTCCGGCCGCGGCGACAAGGACATGCAGACCGTCATGCACCACTAC
GACGAACAGGAAGAACATAT

Downstream 100 bases:

>100_bases
CCGCAACGTTGCCCTGCAGAGCACCGCCGCCCGGTCGATCCGGGCGGCGGTTTTTTTTGCCTGTGTTCTTTGGCAAGGCC
AACTCGGCTGCCGTGCACAG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSRLLTRFDELKQQNRAALVTFITAGDPDYATSLQILKGLPAAGADVIELGMPFTDPMADGPAIQLANIRALAAKQDLAK
TLQMVREFRQDDQTTPIVLMGYFNPIHKMGVERFIAEAVEAGVDGLIVVDLPPEHNEDLCDPAQAAGIDFIRLTTPTTDD
KRLPVVLNGSSGFVYYVSVAGVTGAGSATLEHVEEAVARLKRHTELPVCVGFGIRTPEQAAAIARLTEGVVVGSALIDQI
ANAKSNAQAVEGVLELCRQISTGVRSARA

Sequences:

>Translated_269_residues
MSRLLTRFDELKQQNRAALVTFITAGDPDYATSLQILKGLPAAGADVIELGMPFTDPMADGPAIQLANIRALAAKQDLAK
TLQMVREFRQDDQTTPIVLMGYFNPIHKMGVERFIAEAVEAGVDGLIVVDLPPEHNEDLCDPAQAAGIDFIRLTTPTTDD
KRLPVVLNGSSGFVYYVSVAGVTGAGSATLEHVEEAVARLKRHTELPVCVGFGIRTPEQAAAIARLTEGVVVGSALIDQI
ANAKSNAQAVEGVLELCRQISTGVRSARA
>Mature_268_residues
SRLLTRFDELKQQNRAALVTFITAGDPDYATSLQILKGLPAAGADVIELGMPFTDPMADGPAIQLANIRALAAKQDLAKT
LQMVREFRQDDQTTPIVLMGYFNPIHKMGVERFIAEAVEAGVDGLIVVDLPPEHNEDLCDPAQAAGIDFIRLTTPTTDDK
RLPVVLNGSSGFVYYVSVAGVTGAGSATLEHVEEAVARLKRHTELPVCVGFGIRTPEQAAAIARLTEGVVVGSALIDQIA
NAKSNAQAVEGVLELCRQISTGVRSARA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=219, Percent_Identity=38.3561643835616, Blast_Score=150, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321412, Length=242, Percent_Identity=42.9752066115703, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PSEU5 (A4VNJ1)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173384.1
- STRING:   A4VNJ1
- GeneID:   5095858
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_2896
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   A4VNJ1
- ProtClustDB:   PRK13111
- BioCyc:   PSTU379731:PST_2896-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28690; Mature: 28559

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLTRFDELKQQNRAALVTFITAGDPDYATSLQILKGLPAAGADVIELGMPFTDPMAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCC
GPAIQLANIRALAAKQDLAKTLQMVREFRQDDQTTPIVLMGYFNPIHKMGVERFIAEAVE
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGVDGLIVVDLPPEHNEDLCDPAQAAGIDFIRLTTPTTDDKRLPVVLNGSSGFVYYVSVA
CCCCCEEEEECCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEEEC
GVTGAGSATLEHVEEAVARLKRHTELPVCVGFGIRTPEQAAAIARLTEGVVVGSALIDQI
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ANAKSNAQAVEGVLELCRQISTGVRSARA
HCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLLTRFDELKQQNRAALVTFITAGDPDYATSLQILKGLPAAGADVIELGMPFTDPMAD
CHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCC
GPAIQLANIRALAAKQDLAKTLQMVREFRQDDQTTPIVLMGYFNPIHKMGVERFIAEAVE
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGVDGLIVVDLPPEHNEDLCDPAQAAGIDFIRLTTPTTDDKRLPVVLNGSSGFVYYVSVA
CCCCCEEEEECCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEEEC
GVTGAGSATLEHVEEAVARLKRHTELPVCVGFGIRTPEQAAAIARLTEGVVVGSALIDQI
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ANAKSNAQAVEGVLELCRQISTGVRSARA
HCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA