Click here to switch to the map view.

The map label for this gene is metR [H]

Identifier: 146283187

GI number: 146283187

Start: 3070497

End: 3071423

Strand: Direct

Name: metR [H]

Synonym: PST_2852

Alternate gene names: 146283187

Gene position: 3070497-3071423 (Clockwise)

Preceding gene: 146283186

Following gene: 146283191

Centisome position: 67.23

GC content: 65.8

Gene sequence:

>927_bases
ATGCTGGAAATTCGCCACCTGAAGACGCTTCATGCACTGCGGGAAACCGACAGTCTCGTCGAAGCCGCCGAGCGCCTGCA
CCTGACCCAGTCGGCGCTCTCCCATCAGTTCAAGGAGCTGGAGGAGCGCCTCGGCCTTCAATTGTTCGTGCGCAAGACCC
GCCCGGTGCGCTTCACCAGCGCCGGGTTGCGCTTGCTGCAACTGGCCGACCTGATGCTGCCGCAACTGCGCAGTGCCGAA
CGCGACCTCGCCCGCCTGGCCGGTGGCACGGCCGGCCGCCTGCACATGGCCATCGAATGTCACAGCTGTTTCCAGTGGCT
GATGCCGACCATCGACCAGTTCCGCGACGCCTGGCCCGAAGTGGAGCTGGACCTGGCGTCCGGATTTTCCTTCGCGCCGC
TGCCAGCCCTGGCCCGCGGCGATCTCGACCTGGTGGTGACCTCCGACCCGCTGGAGCTGCCGGGCATCACCTATGTGCCA
TTGTTCACCTACGAGGCGTTGCTCGCGGTGGCTAACCAGCACCCGCTGGCCGCGCGGCCGTACGTACGGCCAGAGGATCT
GGCCAGCGAGACGCTGATCACCTATCCGGTCGAGCGGGATCGCCTGGACATCTTCACCCGCTTCCTCGAACCGGCCGATA
TCGAACCCGCGCAGGTGCGCACCTCGGAGCTCACCGTGATGATGATGCAGCTGGTCGCATCGGGGCGCGGGGTCTGCTGT
CTGCCCAACTGGGCCCTGCACGAGTACAGCTCGCGCGGCTACGTCACCGCCAAGCGTCTCGGCGACAAGGGCCTGTTCGC
CACGCTTTATGCCGGCATTCGCGCAGACATGCTCGATTCGCCGTTCATGCGCGACTTCCTGCTGACGGCCAAAGACACCT
CCTTTGCCACCCTTGAAGGTGTCAGCGCCGCGCCCAAGGGTCGCTGA

Upstream 100 bases:

>100_bases
CACTTGCGAAGGATGAGCCTCGCTCATCTTCACCCTCTTTCTGGGATTCGCTCGCCTGTCGCTCAGCGCGGTGCGATCCC
GCTTCGCAAGGTAGTTCGGC

Downstream 100 bases:

>100_bases
CCCGGGCGTTCAGCGCCGCCGCGTCAGGCACAGGCAGACACCCACGGCCGCCGCCGCGATACCGCCGATCAGTAGCCAGT
GCTTATCGCCATCGACCGCC

Product: transcriptional regulator MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLEIRHLKTLHALRETDSLVEAAERLHLTQSALSHQFKELEERLGLQLFVRKTRPVRFTSAGLRLLQLADLMLPQLRSAE
RDLARLAGGTAGRLHMAIECHSCFQWLMPTIDQFRDAWPEVELDLASGFSFAPLPALARGDLDLVVTSDPLELPGITYVP
LFTYEALLAVANQHPLAARPYVRPEDLASETLITYPVERDRLDIFTRFLEPADIEPAQVRTSELTVMMMQLVASGRGVCC
LPNWALHEYSSRGYVTAKRLGDKGLFATLYAGIRADMLDSPFMRDFLLTAKDTSFATLEGVSAAPKGR

Sequences:

>Translated_308_residues
MLEIRHLKTLHALRETDSLVEAAERLHLTQSALSHQFKELEERLGLQLFVRKTRPVRFTSAGLRLLQLADLMLPQLRSAE
RDLARLAGGTAGRLHMAIECHSCFQWLMPTIDQFRDAWPEVELDLASGFSFAPLPALARGDLDLVVTSDPLELPGITYVP
LFTYEALLAVANQHPLAARPYVRPEDLASETLITYPVERDRLDIFTRFLEPADIEPAQVRTSELTVMMMQLVASGRGVCC
LPNWALHEYSSRGYVTAKRLGDKGLFATLYAGIRADMLDSPFMRDFLLTAKDTSFATLEGVSAAPKGR
>Mature_308_residues
MLEIRHLKTLHALRETDSLVEAAERLHLTQSALSHQFKELEERLGLQLFVRKTRPVRFTSAGLRLLQLADLMLPQLRSAE
RDLARLAGGTAGRLHMAIECHSCFQWLMPTIDQFRDAWPEVELDLASGFSFAPLPALARGDLDLVVTSDPLELPGITYVP
LFTYEALLAVANQHPLAARPYVRPEDLASETLITYPVERDRLDIFTRFLEPADIEPAQVRTSELTVMMMQLVASGRGVCC
LPNWALHEYSSRGYVTAKRLGDKGLFATLYAGIRADMLDSPFMRDFLLTAKDTSFATLEGVSAAPKGR

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=304, Percent_Identity=47.3684210526316, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=29.8245614035088, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=26.962457337884, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=31.3253012048193, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=29.6, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789173, Length=248, Percent_Identity=30.6451612903226, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788296, Length=296, Percent_Identity=28.3783783783784, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=27.710843373494, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=30.5555555555556, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367136, Length=249, Percent_Identity=25.3012048192771, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786401, Length=282, Percent_Identity=26.5957446808511, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693105, Length=191, Percent_Identity=26.1780104712042, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=279, Percent_Identity=24.7311827956989, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=270, Percent_Identity=20.7407407407407, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082024, Length=292, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081904, Length=177, Percent_Identity=31.0734463276836, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=24.6913580246914, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787589, Length=129, Percent_Identity=34.1085271317829, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=23.6686390532544, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=267, Percent_Identity=24.3445692883895, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34450; Mature: 34450

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIRHLKTLHALRETDSLVEAAERLHLTQSALSHQFKELEERLGLQLFVRKTRPVRFTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEHH
AGLRLLQLADLMLPQLRSAERDLARLAGGTAGRLHMAIECHSCFQWLMPTIDQFRDAWPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCC
VELDLASGFSFAPLPALARGDLDLVVTSDPLELPGITYVPLFTYEALLAVANQHPLAARP
EEEECCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCC
YVRPEDLASETLITYPVERDRLDIFTRFLEPADIEPAQVRTSELTVMMMQLVASGRGVCC
CCCHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
LPNWALHEYSSRGYVTAKRLGDKGLFATLYAGIRADMLDSPFMRDFLLTAKDTSFATLEG
CCCHHHHHHCCCCCEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECC
VSAAPKGR
CCCCCCCC
>Mature Secondary Structure
MLEIRHLKTLHALRETDSLVEAAERLHLTQSALSHQFKELEERLGLQLFVRKTRPVRFTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEHH
AGLRLLQLADLMLPQLRSAERDLARLAGGTAGRLHMAIECHSCFQWLMPTIDQFRDAWPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCC
VELDLASGFSFAPLPALARGDLDLVVTSDPLELPGITYVPLFTYEALLAVANQHPLAARP
EEEECCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCC
YVRPEDLASETLITYPVERDRLDIFTRFLEPADIEPAQVRTSELTVMMMQLVASGRGVCC
CCCHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
LPNWALHEYSSRGYVTAKRLGDKGLFATLYAGIRADMLDSPFMRDFLLTAKDTSFATLEG
CCCHHHHHHCCCCCEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECC
VSAAPKGR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]