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The map label for this gene is gph-2 [H]

Identifier: 146282668

GI number: 146282668

Start: 2538689

End: 2539360

Strand: Direct

Name: gph-2 [H]

Synonym: PST_2324

Alternate gene names: 146282668

Gene position: 2538689-2539360 (Clockwise)

Preceding gene: 146282667

Following gene: 146282669

Centisome position: 55.58

GC content: 63.24

Gene sequence:

>672_bases
ATGCGCCTGCGCGCTGTTCTGTTCGACATGGACGGTACCCTGCTCGATACCGCTCCCGATTTCATCGCCGTGGCGCAGGC
CATGCGGATTGCGCGCGGCCTGGAGCCTGTGCCGGATCAACAGATTCGTGACGTGGTGTCCGGTGGTGCCAGAGCCATGG
TGCTCGGCGCCTTCGATGTGGACCCCATGTCGGAAGAGTTCGAAGCATTGCGCCTGGAGTTTCTTGCGCGCTACCAGGAG
CATTGCGCCGTGCATAGCCGCCTCTACGACGGCATGGCGGAACTGCTGGACGAAATCGAGCGATCCAACCTGCGCTGGGG
TGTGGTAACCAACAAGCCGCTGCGTTTCGCCGAACCGATCATGCAGCAGCTGGGCCTGGCGTCCCGCTCTGCCGTGCTGG
TCTGTCCTGATCACGTCGCCAATAGCAAGCCCGACCCCGAGCCGATGATCCTCGCGTGCAAACAGCTCGACCTTGATCCT
TCTGCGGTCCTGTTCGTCGGTGATGACCTGCGCGACATCGAATCGGGACGAGCGGCGGGCAGCAGGACGGCCGCGGTGCG
CTATGGCTATATCCATCCGCAAGACAACCCGAGCAATTGGGGCGCGGACGTAGTGGTGGATCACCCGCTGGATCTGCGCC
AGGTCCTGGACAGCGCCCTGTGCAGTTGCTGA

Upstream 100 bases:

>100_bases
AGGACATCATCGGCCTTACCTATAACCCGCTGACCAAGCACTACAAGCTGTCGGCGGACGTCGACGTCAACTACATGGTC
CAGACCGTCAAGGAGTCCTG

Downstream 100 bases:

>100_bases
AACAGCCATAGGGTCACCACCACAGCATCATCACTCGGCAGTAATTGCCGAAGCACCCATCACTTTCGATTTACGAGGCA
TCCGATGTTCGAGTATTCCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRLRAVLFDMDGTLLDTAPDFIAVAQAMRIARGLEPVPDQQIRDVVSGGARAMVLGAFDVDPMSEEFEALRLEFLARYQE
HCAVHSRLYDGMAELLDEIERSNLRWGVVTNKPLRFAEPIMQQLGLASRSAVLVCPDHVANSKPDPEPMILACKQLDLDP
SAVLFVGDDLRDIESGRAAGSRTAAVRYGYIHPQDNPSNWGADVVVDHPLDLRQVLDSALCSC

Sequences:

>Translated_223_residues
MRLRAVLFDMDGTLLDTAPDFIAVAQAMRIARGLEPVPDQQIRDVVSGGARAMVLGAFDVDPMSEEFEALRLEFLARYQE
HCAVHSRLYDGMAELLDEIERSNLRWGVVTNKPLRFAEPIMQQLGLASRSAVLVCPDHVANSKPDPEPMILACKQLDLDP
SAVLFVGDDLRDIESGRAAGSRTAAVRYGYIHPQDNPSNWGADVVVDHPLDLRQVLDSALCSC
>Mature_223_residues
MRLRAVLFDMDGTLLDTAPDFIAVAQAMRIARGLEPVPDQQIRDVVSGGARAMVLGAFDVDPMSEEFEALRLEFLARYQE
HCAVHSRLYDGMAELLDEIERSNLRWGVVTNKPLRFAEPIMQQLGLASRSAVLVCPDHVANSKPDPEPMILACKQLDLDP
SAVLFVGDDLRDIESGRAAGSRTAAVRYGYIHPQDNPSNWGADVVVDHPLDLRQVLDSALCSC

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=238, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24570; Mature: 24570

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRAVLFDMDGTLLDTAPDFIAVAQAMRIARGLEPVPDQQIRDVVSGGARAMVLGAFDV
CCCEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCC
DPMSEEFEALRLEFLARYQEHCAVHSRLYDGMAELLDEIERSNLRWGVVTNKPLRFAEPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
MQQLGLASRSAVLVCPDHVANSKPDPEPMILACKQLDLDPSAVLFVGDDLRDIESGRAAG
HHHHCCCCCCEEEECCHHHCCCCCCCCHHEEEEHHCCCCCCEEEEECCCHHHHHCCCCCC
SRTAAVRYGYIHPQDNPSNWGADVVVDHPLDLRQVLDSALCSC
CCCEEEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRLRAVLFDMDGTLLDTAPDFIAVAQAMRIARGLEPVPDQQIRDVVSGGARAMVLGAFDV
CCCEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCC
DPMSEEFEALRLEFLARYQEHCAVHSRLYDGMAELLDEIERSNLRWGVVTNKPLRFAEPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
MQQLGLASRSAVLVCPDHVANSKPDPEPMILACKQLDLDPSAVLFVGDDLRDIESGRAAG
HHHHCCCCCCEEEECCHHHCCCCCCCCHHEEEEHHCCCCCCEEEEECCCHHHHHCCCCCC
SRTAAVRYGYIHPQDNPSNWGADVVVDHPLDLRQVLDSALCSC
CCCEEEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]