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The map label for this gene is glnG [H]

Identifier: 145300970

GI number: 145300970

Start: 4415542

End: 4416960

Strand: Direct

Name: glnG [H]

Synonym: ASA_4123

Alternate gene names: 145300970

Gene position: 4415542-4416960 (Clockwise)

Preceding gene: 145300969

Following gene: 145300971

Centisome position: 93.9

GC content: 62.79

Gene sequence:

>1419_bases
ATGACAGCAAAAGTGTGGATCGTCGATGACGACAGTTCTATCCGCTGGGTACTGGAACGCACCCTCGGCAGTGAAGGCTT
CGAGTGCGAGAGTTTTGCCGATGGCGAGGCTCTGCTGAGCTCGCTGGAGATACGTTCGCCCGATGTGATCCTCTCCGATA
TCCGCATGCCCGGCATCGATGGTCTCAGCCTGCTGGAGCAGATCCACCGCCGCCAGGCCGATCTGCCCATCATCATAATG
ACGGCCCACTCGGATCTGGACAGCGCAGTCAATGCCTATCAGCGGGGCGCCTTCGAGTATCTGCCCAAGCCGTTCGACAT
CGACGAGGCGGTCACCCTGGTGCGCCGCGCCGAGAATCACCTGAAAGAACAGCGCACCAAGCGCAAGGCACGCCAGCAGG
AGACGGCCACCACACCTGAAATCATCGGCGAAGCGCCGGCCATGCAGGAAGTGTTTCGCGCCATCGGCCGGCTGTCGCGC
TCCAGCATCTCAGTGCTGATCAACGGTCAGAGCGGTACCGGCAAGGAGCTGGTCGCCCACGCGCTGCACAAGCACAGCCC
GCGCGCCCACAAGAACTTCATCGCACTCAACATGGCCGCCATCCCCAAAGATCTCATCGAATCCGAGCTGTTCGGTCATG
AAAAAGGGGCCTTTACCGGTGCCAACAATATTCGCCACGGCCGCTTCGAGCAGGCCGATGGCGGCACCCTGTTTCTCGAT
GAAATCGGCGACATGCCGCTGGATGTGCAGACCCGCCTGCTGCGGGTGCTGGCCGATGGCCAGTTCTACCGGGTCGGCGG
TCATCAGCCGGTGCAGGTAGATGTGCGGATCATCGCCGCTACCCACCAGAATCTGGAGAAACGGGTGGCAGACGGTGAAT
TCCGGGAAGATCTGTTCCACCGCCTCAACGTCATCCGCATTCATATTCCGGCCCTCAAGGAGCGCCGCGAGGATATACCT
CAACTGGCCCAGCACTTCCTGCTGCGCGCCGCCAAGGAGCTGAACGTGGAGGCCAAGAGCCTGCACCCGGATACCCAGGC
CTACATCAGCCGTCTCGCCTGGCCGGGCAACGTGCGCCAGCTGGAGAACGTCTGCCGCTGGCTGACGGTCATGGCCTCGG
GCCAGGAGGTGCTGGTAGGGGATCTGCCCGCCGAACTGCTGACCCCCATCACCAACACGGCCGAGCCCGGCCAACCTGCC
CTGCCCGGCTGCTGGCAGCAGCAGTTGCAGGACTGGGTCGCCAGTAAGCTGGCACTGGGGGAGACCGACATCCTGGCCGA
TGCCCTGCCCCAATTTGAGCGGATCATGCTGGAGACGGCACTGGATCACACCCATGGCCACAAACAGGAGGCTGCCCGCC
TGCTTGGCTGGGGTCGCAACACCCTGACTCGCAAGCTCAAGGAGCTCGACCTCTCCTGA

Upstream 100 bases:

>100_bases
GAATCTGATTGATCAGCACAAGGGGCGTATCGACTGCATCAGCTGGCCTGGTCACACCGAATTCACCATTTTTCTACCGC
TTCGCAAATAAGGGAACGTC

Downstream 100 bases:

>100_bases
GCCCTCTGGAAAAACGATTCCCATCACGCCTTGGCAAATCCCTGACCAATAAGACCCCGTTTGATTTCAAACGGGGTATC
TTTTTTGAAAGCCCTGCCTA

Product: two-component system nitrogen response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTAKVWIVDDDSSIRWVLERTLGSEGFECESFADGEALLSSLEIRSPDVILSDIRMPGIDGLSLLEQIHRRQADLPIIIM
TAHSDLDSAVNAYQRGAFEYLPKPFDIDEAVTLVRRAENHLKEQRTKRKARQQETATTPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAHALHKHSPRAHKNFIALNMAAIPKDLIESELFGHEKGAFTGANNIRHGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHQPVQVDVRIIAATHQNLEKRVADGEFREDLFHRLNVIRIHIPALKERREDIP
QLAQHFLLRAAKELNVEAKSLHPDTQAYISRLAWPGNVRQLENVCRWLTVMASGQEVLVGDLPAELLTPITNTAEPGQPA
LPGCWQQQLQDWVASKLALGETDILADALPQFERIMLETALDHTHGHKQEAARLLGWGRNTLTRKLKELDLS

Sequences:

>Translated_472_residues
MTAKVWIVDDDSSIRWVLERTLGSEGFECESFADGEALLSSLEIRSPDVILSDIRMPGIDGLSLLEQIHRRQADLPIIIM
TAHSDLDSAVNAYQRGAFEYLPKPFDIDEAVTLVRRAENHLKEQRTKRKARQQETATTPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAHALHKHSPRAHKNFIALNMAAIPKDLIESELFGHEKGAFTGANNIRHGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHQPVQVDVRIIAATHQNLEKRVADGEFREDLFHRLNVIRIHIPALKERREDIP
QLAQHFLLRAAKELNVEAKSLHPDTQAYISRLAWPGNVRQLENVCRWLTVMASGQEVLVGDLPAELLTPITNTAEPGQPA
LPGCWQQQLQDWVASKLALGETDILADALPQFERIMLETALDHTHGHKQEAARLLGWGRNTLTRKLKELDLS
>Mature_471_residues
TAKVWIVDDDSSIRWVLERTLGSEGFECESFADGEALLSSLEIRSPDVILSDIRMPGIDGLSLLEQIHRRQADLPIIIMT
AHSDLDSAVNAYQRGAFEYLPKPFDIDEAVTLVRRAENHLKEQRTKRKARQQETATTPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVAHALHKHSPRAHKNFIALNMAAIPKDLIESELFGHEKGAFTGANNIRHGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHQPVQVDVRIIAATHQNLEKRVADGEFREDLFHRLNVIRIHIPALKERREDIPQ
LAQHFLLRAAKELNVEAKSLHPDTQAYISRLAWPGNVRQLENVCRWLTVMASGQEVLVGDLPAELLTPITNTAEPGQPAL
PGCWQQQLQDWVASKLALGETDILADALPQFERIMLETALDHTHGHKQEAARLLGWGRNTLTRKLKELDLS

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=71.7344753747323, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=40.4210526315789, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=40.1727861771058, Blast_Score=321, Evalue=6e-89,
Organism=Escherichia coli, GI1788905, Length=470, Percent_Identity=39.1489361702128, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=43.4131736526946, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=331, Percent_Identity=43.5045317220544, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=47.4789915966387, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1786524, Length=341, Percent_Identity=41.3489736070381, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1787583, Length=366, Percent_Identity=36.8852459016393, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87082152, Length=342, Percent_Identity=38.3040935672515, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=44.9781659388646, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=31.2903225806452, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=36.3636363636364, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1789809, Length=112, Percent_Identity=33.9285714285714, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788394, Length=144, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=122, Percent_Identity=31.9672131147541, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=32.0754716981132, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52826; Mature: 52695

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKVWIVDDDSSIRWVLERTLGSEGFECESFADGEALLSSLEIRSPDVILSDIRMPGID
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCC
GLSLLEQIHRRQADLPIIIMTAHSDLDSAVNAYQRGAFEYLPKPFDIDEAVTLVRRAENH
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHH
LKEQRTKRKARQQETATTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAH
HHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
ALHKHSPRAHKNFIALNMAAIPKDLIESELFGHEKGAFTGANNIRHGRFEQADGGTLFLD
HHHHCCCCHHCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRVGGHQPVQVDVRIIAATHQNLEKRVADGEFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHCCCHHHHHHHH
RLNVIRIHIPALKERREDIPQLAQHFLLRAAKELNVEAKSLHPDTQAYISRLAWPGNVRQ
HHCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCCHHH
LENVCRWLTVMASGQEVLVGDLPAELLTPITNTAEPGQPALPGCWQQQLQDWVASKLALG
HHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
ETDILADALPQFERIMLETALDHTHGHKQEAARLLGWGRNTLTRKLKELDLS
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAKVWIVDDDSSIRWVLERTLGSEGFECESFADGEALLSSLEIRSPDVILSDIRMPGID
CEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCC
GLSLLEQIHRRQADLPIIIMTAHSDLDSAVNAYQRGAFEYLPKPFDIDEAVTLVRRAENH
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHH
LKEQRTKRKARQQETATTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAH
HHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
ALHKHSPRAHKNFIALNMAAIPKDLIESELFGHEKGAFTGANNIRHGRFEQADGGTLFLD
HHHHCCCCHHCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRVGGHQPVQVDVRIIAATHQNLEKRVADGEFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHCCCHHHHHHHH
RLNVIRIHIPALKERREDIPQLAQHFLLRAAKELNVEAKSLHPDTQAYISRLAWPGNVRQ
HHCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCCHHH
LENVCRWLTVMASGQEVLVGDLPAELLTPITNTAEPGQPALPGCWQQQLQDWVASKLALG
HHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
ETDILADALPQFERIMLETALDHTHGHKQEAARLLGWGRNTLTRKLKELDLS
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]