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The map label for this gene is emrA [H]

Identifier: 145300835

GI number: 145300835

Start: 4269601

End: 4270653

Strand: Direct

Name: emrA [H]

Synonym: ASA_3977

Alternate gene names: 145300835

Gene position: 4269601-4270653 (Clockwise)

Preceding gene: 145300833

Following gene: 145300836

Centisome position: 90.8

GC content: 63.63

Gene sequence:

>1053_bases
ATGAGCCAACACAAGAAAATCCCCCTGCTGGCCGCCATGGTGCTGGCCCTGCTTGGCCTGCTGTTCGCCGGTTACTGGTA
TCTGCATGGCCGCTATTTCGAGAGTACCGACAACGCCTACCTGCAGAGCGAAGTGACCGGGATCGGATCCAAGCTGCCCG
GCTACATCAGTGAGGTGCTGGTCACCGACAACCAGGCGGTGAAGGCCGGTCAGCTGATCGCCCGACTGGACGATCGGGAG
TACAAGACCAGGGTGGCCGAGGCCGAGGCCGACCTGCACCTCAATCAGGCGACCGCCGAGAATCTGGCCGCGACCCGGAC
CCAGCAACTGAGCCTGATCCGTCAGGCCGAGGCCAAGGTCGCCTCCGCCAATGCCGAGCAGGTGCGCGCCAGACAACAGG
TGCAAAGGGTCAGCCAGCTCAACCAGCGCCATTACAGCTCGCAAGACAGCCTGGATGAAGTGCGGGCCGGACTGCAGGTC
AATCAGGCGCAGGAACAGGAAGCCAGGGCCGCCCTGCAAGCCGCCCGTGAACGCCTGCTGGTGCTGGATGCCGAGGCCAA
GCAGAATCAGGCCAGGCTGGCATTGAGCGAGGCGCAGTTGGAACAGGCCAGACTGGAGCTGAGCTACACCGAGTTGCAGG
CACCGGCTGACGGCATCATAGGAAAACGCAGTCTGCGGGCAGGGCTCTACGTTCAGTCCGGCATGCAGGTCGCCAGTCTG
GTACCGCTGCAACAGGTGTGGGTCGAGGCCAATTTCAAGGAGACCCAGCTGCACCATATGCAACCGGGCCAGAAGGTCAA
AGTGATCCTTGACGCCTATCCGGATCAACCGGTCGAGGGGATCATCGACAGTCTGGCCCCCGCCACCGGCGCCAGGTTCG
CCCTGCTGCCACCGGAGAATGCCACCGGCAACTTCACCAAGATTGTGCAGCGGGTACCGGTCAAGATCCGCATCCCCGAG
CCGGGTGCCCTGGCAGGCAAGCTGCTGCCGGGCCTCTCCACCGAGGTGATAGTGGATACCCGTGACCCTGCCGCCCTGGT
GGCAGCCAACTGA

Upstream 100 bases:

>100_bases
CCATTGTTGCACCAGTGAAACTGTATATCTCATTAATGGATACTAATCGAAACAGTTGGCTAGTGTTAGCCTGCGCTGCT
TAAGTTCAGGAATCCCCAAA

Downstream 100 bases:

>100_bases
TGGAACCCATTCCACGTCGCAACTGGATCGCCGTCATGGGGGGCCTCATTGGCGCCTTCATGGCCATCCTCGACATCCAG
ATCACCAACGCCTCCCTAAA

Product: multidrug resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSQHKKIPLLAAMVLALLGLLFAGYWYLHGRYFESTDNAYLQSEVTGIGSKLPGYISEVLVTDNQAVKAGQLIARLDDRE
YKTRVAEAEADLHLNQATAENLAATRTQQLSLIRQAEAKVASANAEQVRARQQVQRVSQLNQRHYSSQDSLDEVRAGLQV
NQAQEQEARAALQAARERLLVLDAEAKQNQARLALSEAQLEQARLELSYTELQAPADGIIGKRSLRAGLYVQSGMQVASL
VPLQQVWVEANFKETQLHHMQPGQKVKVILDAYPDQPVEGIIDSLAPATGARFALLPPENATGNFTKIVQRVPVKIRIPE
PGALAGKLLPGLSTEVIVDTRDPAALVAAN

Sequences:

>Translated_350_residues
MSQHKKIPLLAAMVLALLGLLFAGYWYLHGRYFESTDNAYLQSEVTGIGSKLPGYISEVLVTDNQAVKAGQLIARLDDRE
YKTRVAEAEADLHLNQATAENLAATRTQQLSLIRQAEAKVASANAEQVRARQQVQRVSQLNQRHYSSQDSLDEVRAGLQV
NQAQEQEARAALQAARERLLVLDAEAKQNQARLALSEAQLEQARLELSYTELQAPADGIIGKRSLRAGLYVQSGMQVASL
VPLQQVWVEANFKETQLHHMQPGQKVKVILDAYPDQPVEGIIDSLAPATGARFALLPPENATGNFTKIVQRVPVKIRIPE
PGALAGKLLPGLSTEVIVDTRDPAALVAAN
>Mature_349_residues
SQHKKIPLLAAMVLALLGLLFAGYWYLHGRYFESTDNAYLQSEVTGIGSKLPGYISEVLVTDNQAVKAGQLIARLDDREY
KTRVAEAEADLHLNQATAENLAATRTQQLSLIRQAEAKVASANAEQVRARQQVQRVSQLNQRHYSSQDSLDEVRAGLQVN
QAQEQEARAALQAARERLLVLDAEAKQNQARLALSEAQLEQARLELSYTELQAPADGIIGKRSLRAGLYVQSGMQVASLV
PLQQVWVEANFKETQLHHMQPGQKVKVILDAYPDQPVEGIIDSLAPATGARFALLPPENATGNFTKIVQRVPVKIRIPEP
GALAGKLLPGLSTEVIVDTRDPAALVAAN

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=339, Percent_Identity=30.9734513274336, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789041, Length=347, Percent_Identity=30.2593659942363, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790519, Length=293, Percent_Identity=27.6450511945392, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081951, Length=343, Percent_Identity=25.0728862973761, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790024, Length=245, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789900, Length=326, Percent_Identity=25.1533742331288, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790012, Length=243, Percent_Identity=27.5720164609054, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787013, Length=277, Percent_Identity=27.0758122743682, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789637, Length=312, Percent_Identity=24.6794871794872, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38274; Mature: 38143

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHKKIPLLAAMVLALLGLLFAGYWYLHGRYFESTDNAYLQSEVTGIGSKLPGYISEVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHH
VTDNQAVKAGQLIARLDDREYKTRVAEAEADLHLNQATAENLAATRTQQLSLIRQAEAKV
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHH
ASANAEQVRARQQVQRVSQLNQRHYSSQDSLDEVRAGLQVNQAQEQEARAALQAARERLL
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHEE
VLDAEAKQNQARLALSEAQLEQARLELSYTELQAPADGIIGKRSLRAGLYVQSGMQVASL
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHCCCHHHHH
VPLQQVWVEANFKETQLHHMQPGQKVKVILDAYPDQPVEGIIDSLAPATGARFALLPPEN
HHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCC
ATGNFTKIVQRVPVKIRIPEPGALAGKLLPGLSTEVIVDTRDPAALVAAN
CCCHHHHHHHHCCEEEECCCCCCHHHHHCCCCCCEEEEECCCCCEEEECC
>Mature Secondary Structure 
SQHKKIPLLAAMVLALLGLLFAGYWYLHGRYFESTDNAYLQSEVTGIGSKLPGYISEVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHH
VTDNQAVKAGQLIARLDDREYKTRVAEAEADLHLNQATAENLAATRTQQLSLIRQAEAKV
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHH
ASANAEQVRARQQVQRVSQLNQRHYSSQDSLDEVRAGLQVNQAQEQEARAALQAARERLL
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHEE
VLDAEAKQNQARLALSEAQLEQARLELSYTELQAPADGIIGKRSLRAGLYVQSGMQVASL
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHCCCHHHHH
VPLQQVWVEANFKETQLHHMQPGQKVKVILDAYPDQPVEGIIDSLAPATGARFALLPPEN
HHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCC
ATGNFTKIVQRVPVKIRIPEPGALAGKLLPGLSTEVIVDTRDPAALVAAN
CCCHHHHHHHHCCEEEECCCCCCHHHHHCCCCCCEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]