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The map label for this gene is gltT [H]

Identifier: 145300833

GI number: 145300833

Start: 4266944

End: 4268161

Strand: Direct

Name: gltT [H]

Synonym: ASA_3974

Alternate gene names: 145300833

Gene position: 4266944-4268161 (Clockwise)

Preceding gene: 145300830

Following gene: 145300835

Centisome position: 90.74

GC content: 61.66

Gene sequence:

>1218_bases
TTGAAGACCCTGATCGGCATGTTGACGGGGATCGGGCTGGGACTCGGCTTCAGTGAGCAGGCGTTGCAGCTCAAGCCCCT
TGGCGTCCTGTTCGTCAACACCATACAGATGCTGATGGTGCCGCTCATCTTCTGTTCTGTCATCGCCGGCCTCACCTCCC
TGCTCAAGGGCAAGAGCCTGGATCGGATCGGCAGCAAGGCCATAGGCCTCTATCTCTTTTCCACCGCCATCGCCATCTGC
ATCGGTCTGATGATGGGCTGGCTGCTGGAACCCGGCATGGGCGCCAACATGGGGCCCTATGAGCGGGTCGGCATGGCGGA
ACAGCCAACCCTGGCCAGCGTGCTGAGCCATCTGGTACCACGCAACCCTGTGCAGGCTATGGTGGATGGCAAGATACTGC
AGGTGATCGTTTTTGCGGTCGCCCTCGCCCTCAACGCCACAGGCCGACGCGGTCGCCCCGCCATCCTCTTCTTTACCTCG
CTGGCAGAGGGAATGTTCAAGCTGACCCAGATGGTCATGGCGCTGGCGCCCTATGGCGTCTGCGCCCTGATGGCCTGGAT
GACGGGCAAATACGGGGTGAGTCTGCTCTTGCCGCTGCTCAAGGTGATCGGCGCCGTCTATCTCGGCTGCCTGCTCCATG
TGCTTGGCGTCTATAGCAGCCTGCTTATCCTGCTGGCACGGCTCAACCCGCTGCACTACTTCAAGAGCATCGTCGATGCC
CAGGCCGTGGCCTTCACCAGCAGCTCCAGCAGCAGCACACTGCCCATCAGTCTGGCCTGCGCCGAGAAGCGGCTCGGGGT
CTCACCCGCCATCACCGCCAAGGTGCTGCCCATCGGCGCCACCATCAATATGGACGGCACCGCCCTCTACCAGGGAGTCA
CAGCCCTATTCGTGGCCCAAGCATTTGGCGTGGATCTGCACATGGTGGATTACCTGACCATCATCAGCATCGCCACCCTG
GCCAGTATCGGTACCGCAGGCACACCGGGCACAGGGCTCATGTTGCTGACCCTGACGCTCACTGCGGTAGGGTTGCCGCT
GGAAGGAGTAGCCCTCATCGCCGGGATCGACAGGATCCTCGACATGGCGCGTACCACGGTCAACGTGTCCGGCGATATAC
TGGTATCCGTGCTGATTGCTCGCAGCGAGAAGGAGCTGGATCTGGCGACCTATCACGACGCCAGCGCAGGAGATGATATC
GACTTCACCCTGCGCTGA

Upstream 100 bases:

>100_bases
CTTTTTGCCATCGCAGACAGAATATAAGGACATTGGCGTCATGCTGTTCCATGGATTGGCTTTCAAGCCCTGGCGGATCT
GGACCCGCCGTCCCCTCTGG

Downstream 100 bases:

>100_bases
GGCAGGGTGAAGGATTAACTATCGTGGGGATCGTGATGACAGCGCCAGCAGACCTCAAAGTTGGCGCCGTTTTCCTCCCC
GCACTGACCGCAGAGCCAAC

Product: proton/glutamate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKTLIGMLTGIGLGLGFSEQALQLKPLGVLFVNTIQMLMVPLIFCSVIAGLTSLLKGKSLDRIGSKAIGLYLFSTAIAIC
IGLMMGWLLEPGMGANMGPYERVGMAEQPTLASVLSHLVPRNPVQAMVDGKILQVIVFAVALALNATGRRGRPAILFFTS
LAEGMFKLTQMVMALAPYGVCALMAWMTGKYGVSLLLPLLKVIGAVYLGCLLHVLGVYSSLLILLARLNPLHYFKSIVDA
QAVAFTSSSSSSTLPISLACAEKRLGVSPAITAKVLPIGATINMDGTALYQGVTALFVAQAFGVDLHMVDYLTIISIATL
ASIGTAGTPGTGLMLLTLTLTAVGLPLEGVALIAGIDRILDMARTTVNVSGDILVSVLIARSEKELDLATYHDASAGDDI
DFTLR

Sequences:

>Translated_405_residues
MKTLIGMLTGIGLGLGFSEQALQLKPLGVLFVNTIQMLMVPLIFCSVIAGLTSLLKGKSLDRIGSKAIGLYLFSTAIAIC
IGLMMGWLLEPGMGANMGPYERVGMAEQPTLASVLSHLVPRNPVQAMVDGKILQVIVFAVALALNATGRRGRPAILFFTS
LAEGMFKLTQMVMALAPYGVCALMAWMTGKYGVSLLLPLLKVIGAVYLGCLLHVLGVYSSLLILLARLNPLHYFKSIVDA
QAVAFTSSSSSSTLPISLACAEKRLGVSPAITAKVLPIGATINMDGTALYQGVTALFVAQAFGVDLHMVDYLTIISIATL
ASIGTAGTPGTGLMLLTLTLTAVGLPLEGVALIAGIDRILDMARTTVNVSGDILVSVLIARSEKELDLATYHDASAGDDI
DFTLR
>Mature_405_residues
MKTLIGMLTGIGLGLGFSEQALQLKPLGVLFVNTIQMLMVPLIFCSVIAGLTSLLKGKSLDRIGSKAIGLYLFSTAIAIC
IGLMMGWLLEPGMGANMGPYERVGMAEQPTLASVLSHLVPRNPVQAMVDGKILQVIVFAVALALNATGRRGRPAILFFTS
LAEGMFKLTQMVMALAPYGVCALMAWMTGKYGVSLLLPLLKVIGAVYLGCLLHVLGVYSSLLILLARLNPLHYFKSIVDA
QAVAFTSSSSSSTLPISLACAEKRLGVSPAITAKVLPIGATINMDGTALYQGVTALFVAQAFGVDLHMVDYLTIISIATL
ASIGTAGTPGTGLMLLTLTLTAVGLPLEGVALIAGIDRILDMARTTVNVSGDILVSVLIARSEKELDLATYHDASAGDDI
DFTLR

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI66773030, Length=403, Percent_Identity=31.7617866004963, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI40254478, Length=453, Percent_Identity=29.8013245033113, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5032093, Length=379, Percent_Identity=35.3562005277045, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI169790839, Length=415, Percent_Identity=32.0481927710843, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI21314632, Length=392, Percent_Identity=30.8673469387755, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI223468566, Length=260, Percent_Identity=40.3846153846154, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4827012, Length=278, Percent_Identity=37.7697841726619, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI223468564, Length=251, Percent_Identity=40.2390438247012, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI194239697, Length=260, Percent_Identity=36.1538461538462, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI301601644, Length=173, Percent_Identity=39.3063583815029, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI262359914, Length=355, Percent_Identity=29.0140845070423, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1790514, Length=383, Percent_Identity=36.5535248041775, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789947, Length=407, Percent_Identity=33.6609336609337, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788024, Length=383, Percent_Identity=28.9817232375979, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1789473, Length=277, Percent_Identity=24.9097472924188, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71996953, Length=453, Percent_Identity=32.6710816777042, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI71983106, Length=441, Percent_Identity=31.0657596371882, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71983099, Length=441, Percent_Identity=31.0657596371882, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17537407, Length=392, Percent_Identity=31.3775510204082, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI193206505, Length=441, Percent_Identity=29.7052154195011, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193206654, Length=439, Percent_Identity=28.0182232346241, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17541374, Length=393, Percent_Identity=28.2442748091603, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24583025, Length=435, Percent_Identity=29.4252873563218, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137668, Length=435, Percent_Identity=29.4252873563218, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24583023, Length=435, Percent_Identity=29.4252873563218, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281360483, Length=263, Percent_Identity=34.2205323193916, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137666, Length=263, Percent_Identity=34.2205323193916, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281360481, Length=263, Percent_Identity=34.2205323193916, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 42531; Mature: 42531

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLIGMLTGIGLGLGFSEQALQLKPLGVLFVNTIQMLMVPLIFCSVIAGLTSLLKGKSL
CHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DRIGSKAIGLYLFSTAIAICIGLMMGWLLEPGMGANMGPYERVGMAEQPTLASVLSHLVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCC
RNPVQAMVDGKILQVIVFAVALALNATGRRGRPAILFFTSLAEGMFKLTQMVMALAPYGV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHH
CALMAWMTGKYGVSLLLPLLKVIGAVYLGCLLHVLGVYSSLLILLARLNPLHYFKSIVDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QAVAFTSSSSSSTLPISLACAEKRLGVSPAITAKVLPIGATINMDGTALYQGVTALFVAQ
HHHEEECCCCCCCCEEEEEEHHHHCCCCCCHHEEEEEECEEECCCCHHHHHHHHHHHHHH
AFGVDLHMVDYLTIISIATLASIGTAGTPGTGLMLLTLTLTAVGLPLEGVALIAGIDRIL
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DMARTTVNVSGDILVSVLIARSEKELDLATYHDASAGDDIDFTLR
HHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEC
>Mature Secondary Structure
MKTLIGMLTGIGLGLGFSEQALQLKPLGVLFVNTIQMLMVPLIFCSVIAGLTSLLKGKSL
CHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DRIGSKAIGLYLFSTAIAICIGLMMGWLLEPGMGANMGPYERVGMAEQPTLASVLSHLVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCC
RNPVQAMVDGKILQVIVFAVALALNATGRRGRPAILFFTSLAEGMFKLTQMVMALAPYGV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHH
CALMAWMTGKYGVSLLLPLLKVIGAVYLGCLLHVLGVYSSLLILLARLNPLHYFKSIVDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QAVAFTSSSSSSTLPISLACAEKRLGVSPAITAKVLPIGATINMDGTALYQGVTALFVAQ
HHHEEECCCCCCCCEEEEEEHHHHCCCCCCHHEEEEEECEEECCCCHHHHHHHHHHHHHH
AFGVDLHMVDYLTIISIATLASIGTAGTPGTGLMLLTLTLTAVGLPLEGVALIAGIDRIL
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DMARTTVNVSGDILVSVLIARSEKELDLATYHDASAGDDIDFTLR
HHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]