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The map label for this gene is emrB [H]

Identifier: 145300836

GI number: 145300836

Start: 4270653

End: 4272206

Strand: Direct

Name: emrB [H]

Synonym: ASA_3978

Alternate gene names: 145300836

Gene position: 4270653-4272206 (Clockwise)

Preceding gene: 145300835

Following gene: 145300838

Centisome position: 90.82

GC content: 61.78

Gene sequence:

>1554_bases
ATGGAACCCATTCCACGTCGCAACTGGATCGCCGTCATGGGGGGCCTCATTGGCGCCTTCATGGCCATCCTCGACATCCA
GATCACCAACGCCTCCCTAAAGGATATTCAGGGGGCCCTGTCGGCCACCCTGAGCGAGAGCTCCTGGATCTCCACCTCCT
ATCTGGTGGCGGAGATGATCGCCATTCCCCTGAGCGGCTGGCTGAGTCGGGGTCTGAGTGTGCGTCGTTACCTGTTGTGG
ACCACGGGGGCCTTTATCGTCGCCTCCGTGCTCTGCTCGTTCAGCTGGAACCTCACCAGCATGATCGTCTTCCGGGCGCT
GCAGGGTTTCACCGGCGGGGCGCTGATCCCCATGGCCTTCTCCCTCATCGTCAGCCTGCTGCCACTGCACAAGCGAGCGA
CCGGCATGGCACTGTTCGGCCTCTGTGCCACCTTCGCCCCCGCCATAGGTCCGACTCTGGGTGGCTGGCTGACGGAGCAG
CTCTCCTGGCACTACATCTTCTACCTGAACGTACCGCCCGGCCTGCTGGTGATGGCCATGCTGGCCCATGGTCTGGACAA
GAAGCCGGTCGACTGGACAGTGATCAAGCAGGTGGATCTCATCGGCATCATCACAATGGCGATGGGACTGGGTCTGCTGG
AAGTGGTGCTGGAAGAGGGCAACAGAGAGGACTGGTTTGGCTCGAGCTTCATAGTGCGGCTCACCGTCATCTCTGTGGTG
GCCCTGGTGTTCTTCGTCATCAGCCAGCTGCTCAGACGCCATCCGCTGGTCAACCTGCGGCTGCTGCACAACCCGCGCTT
CGCGCTGGCCTGCTTTGCCTATCTGGTGCTCGGCATGGCGCTAATGGGCTCCATTTATGTGCTGCCGCTCTACATGGCGC
AGATCCACAACTACAACGCGCTGGAAATCGGTGAGGTGCTGATGTGGATGGGGCTGCCCCAACTGCTGGTGTTGCCGCTG
GTGCCCAAGCTGACCCACAAGATAGACCCACGCTATCTGGTGAGCTTCGGTTTTCTGGTATTTGCCATCAGCTGCTTCAT
GAACATGAACATGAGTGCCGACTACGCCGGCCCCCAGCTTATCCAGTCGCTGATAGTGCGGGCGCTGGGTCAACCCTTCA
TCATGGTGCCGCTCTCTCTGGTGGCGACAGCCAGCCTGAGTTACGACGAGATCCCCTCCTCCTCGACCCTGCTCAACGTG
CTGCGCAACCTGGGGGGTGCCTTTGGCATCGCCATCATAGCGACCCTGATCGACAACGACACCCGCGTCCATGTCAGCCA
GATCAGCAGCACGCTGGCCGCCAGCAGCATAGAGGGGCAGAGCTACCTGCTGCAGCTGGCCCGGAGCATGATGGCGCAGG
GCTCGGGTCCCGAGCTGGCCCAACAGCAGGCCCAGGCCATGCTGTCGAACACCATCAGCCGCGAGGCTGCCATCATGGCG
TACAACCAGGTGTTCTACGTGATGGGGATCTTCCTGCTCATCGCCAGCGCCCTGATGCTGCTGCTCAAGCAGCCAGCCCC
CAGAGCGGCCGATGCCGAGCCGCTCGAGGCATAG

Upstream 100 bases:

>100_bases
TCCCCGAGCCGGGTGCCCTGGCAGGCAAGCTGCTGCCGGGCCTCTCCACCGAGGTGATAGTGGATACCCGTGACCCTGCC
GCCCTGGTGGCAGCCAACTG

Downstream 100 bases:

>100_bases
CAAAGGCGTAAAAACAGACACAAAAAATGGGCCAACAGGCCCATTTTTCTTGCTGGTGATCATCTGCTCGCCGAGCCTGA
GGGAGCAGGTGCAAGTGACG

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MEPIPRRNWIAVMGGLIGAFMAILDIQITNASLKDIQGALSATLSESSWISTSYLVAEMIAIPLSGWLSRGLSVRRYLLW
TTGAFIVASVLCSFSWNLTSMIVFRALQGFTGGALIPMAFSLIVSLLPLHKRATGMALFGLCATFAPAIGPTLGGWLTEQ
LSWHYIFYLNVPPGLLVMAMLAHGLDKKPVDWTVIKQVDLIGIITMAMGLGLLEVVLEEGNREDWFGSSFIVRLTVISVV
ALVFFVISQLLRRHPLVNLRLLHNPRFALACFAYLVLGMALMGSIYVLPLYMAQIHNYNALEIGEVLMWMGLPQLLVLPL
VPKLTHKIDPRYLVSFGFLVFAISCFMNMNMSADYAGPQLIQSLIVRALGQPFIMVPLSLVATASLSYDEIPSSSTLLNV
LRNLGGAFGIAIIATLIDNDTRVHVSQISSTLAASSIEGQSYLLQLARSMMAQGSGPELAQQQAQAMLSNTISREAAIMA
YNQVFYVMGIFLLIASALMLLLKQPAPRAADAEPLEA

Sequences:

>Translated_517_residues
MEPIPRRNWIAVMGGLIGAFMAILDIQITNASLKDIQGALSATLSESSWISTSYLVAEMIAIPLSGWLSRGLSVRRYLLW
TTGAFIVASVLCSFSWNLTSMIVFRALQGFTGGALIPMAFSLIVSLLPLHKRATGMALFGLCATFAPAIGPTLGGWLTEQ
LSWHYIFYLNVPPGLLVMAMLAHGLDKKPVDWTVIKQVDLIGIITMAMGLGLLEVVLEEGNREDWFGSSFIVRLTVISVV
ALVFFVISQLLRRHPLVNLRLLHNPRFALACFAYLVLGMALMGSIYVLPLYMAQIHNYNALEIGEVLMWMGLPQLLVLPL
VPKLTHKIDPRYLVSFGFLVFAISCFMNMNMSADYAGPQLIQSLIVRALGQPFIMVPLSLVATASLSYDEIPSSSTLLNV
LRNLGGAFGIAIIATLIDNDTRVHVSQISSTLAASSIEGQSYLLQLARSMMAQGSGPELAQQQAQAMLSNTISREAAIMA
YNQVFYVMGIFLLIASALMLLLKQPAPRAADAEPLEA
>Mature_517_residues
MEPIPRRNWIAVMGGLIGAFMAILDIQITNASLKDIQGALSATLSESSWISTSYLVAEMIAIPLSGWLSRGLSVRRYLLW
TTGAFIVASVLCSFSWNLTSMIVFRALQGFTGGALIPMAFSLIVSLLPLHKRATGMALFGLCATFAPAIGPTLGGWLTEQ
LSWHYIFYLNVPPGLLVMAMLAHGLDKKPVDWTVIKQVDLIGIITMAMGLGLLEVVLEEGNREDWFGSSFIVRLTVISVV
ALVFFVISQLLRRHPLVNLRLLHNPRFALACFAYLVLGMALMGSIYVLPLYMAQIHNYNALEIGEVLMWMGLPQLLVLPL
VPKLTHKIDPRYLVSFGFLVFAISCFMNMNMSADYAGPQLIQSLIVRALGQPFIMVPLSLVATASLSYDEIPSSSTLLNV
LRNLGGAFGIAIIATLIDNDTRVHVSQISSTLAASSIEGQSYLLQLARSMMAQGSGPELAQQQAQAMLSNTISREAAIMA
YNQVFYVMGIFLLIASALMLLLKQPAPRAADAEPLEA

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=487, Percent_Identity=31.4168377823409, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=28.8270377733598, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=26.2910798122066, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=24.2857142857143, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788392, Length=201, Percent_Identity=29.3532338308458, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=251, Percent_Identity=24.3027888446215, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56372; Mature: 56372

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIPRRNWIAVMGGLIGAFMAILDIQITNASLKDIQGALSATLSESSWISTSYLVAEMI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AIPLSGWLSRGLSVRRYLLWTTGAFIVASVLCSFSWNLTSMIVFRALQGFTGGALIPMAF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
SLIVSLLPLHKRATGMALFGLCATFAPAIGPTLGGWLTEQLSWHYIFYLNVPPGLLVMAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEECCHHHHHHHH
LAHGLDKKPVDWTVIKQVDLIGIITMAMGLGLLEVVLEEGNREDWFGSSFIVRLTVISVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ALVFFVISQLLRRHPLVNLRLLHNPRFALACFAYLVLGMALMGSIYVLPLYMAQIHNYNA
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEIGEVLMWMGLPQLLVLPLVPKLTHKIDPRYLVSFGFLVFAISCFMNMNMSADYAGPQL
HHHHHHHHHHCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IQSLIVRALGQPFIMVPLSLVATASLSYDEIPSSSTLLNVLRNLGGAFGIAIIATLIDND
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCC
TRVHVSQISSTLAASSIEGQSYLLQLARSMMAQGSGPELAQQQAQAMLSNTISREAAIMA
CEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YNQVFYVMGIFLLIASALMLLLKQPAPRAADAEPLEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEPIPRRNWIAVMGGLIGAFMAILDIQITNASLKDIQGALSATLSESSWISTSYLVAEMI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AIPLSGWLSRGLSVRRYLLWTTGAFIVASVLCSFSWNLTSMIVFRALQGFTGGALIPMAF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
SLIVSLLPLHKRATGMALFGLCATFAPAIGPTLGGWLTEQLSWHYIFYLNVPPGLLVMAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEECCHHHHHHHH
LAHGLDKKPVDWTVIKQVDLIGIITMAMGLGLLEVVLEEGNREDWFGSSFIVRLTVISVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ALVFFVISQLLRRHPLVNLRLLHNPRFALACFAYLVLGMALMGSIYVLPLYMAQIHNYNA
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEIGEVLMWMGLPQLLVLPLVPKLTHKIDPRYLVSFGFLVFAISCFMNMNMSADYAGPQL
HHHHHHHHHHCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IQSLIVRALGQPFIMVPLSLVATASLSYDEIPSSSTLLNVLRNLGGAFGIAIIATLIDND
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCC
TRVHVSQISSTLAASSIEGQSYLLQLARSMMAQGSGPELAQQQAQAMLSNTISREAAIMA
CEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YNQVFYVMGIFLLIASALMLLLKQPAPRAADAEPLEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]