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The map label for this gene is purH [H]

Identifier: 145300327

GI number: 145300327

Start: 3738780

End: 3740372

Strand: Direct

Name: purH [H]

Synonym: ASA_3451

Alternate gene names: 145300327

Gene position: 3738780-3740372 (Clockwise)

Preceding gene: 145300323

Following gene: 145300328

Centisome position: 79.51

GC content: 61.08

Gene sequence:

>1593_bases
ATGGAACAAGCTCGACCCATTCGCCGTGCACTGCTGAGTGTGTCTGACAAGACTGGCATCCTGGAATTTGCCAAGGCACT
CAACGAACGTGGCGTGGCCCTGCTCTCCACTGGCGGCACCGCCAAGCTGCTCGGCGACGCGGGTCTGCCGGTGACCGAGG
TCTCCGACTACACGGGTTTCCCTGAAATGATGGATGGTCGGGTCAAGACCCTGCATCCCAAGGTTCATGGCGGCATTCTC
GGCCGTCGGGATCAGGATGACGCCATCATGGCCCAGCACAATATCTACCCCATCGACATGGTTGTCGTCAACCTCTACCC
CTTTGCCGCTACCGTGGCCAAAGCCGGTTGTTCCCTGGCCGATGCAGTCGAAAACATCGATATCGGCGGCCCGACCATGG
TGCGCTCCGCCGCCAAGAACCACAAAGACGTGGCAATCGTGGTCAAGGCAGCCGACTACGCACGCGTGATCGCCGAGATG
GATGCCAACGGCAACAGCCTCAAACTGGCTACCCGTTTTGACCTTGCCATTGCCGCCTTCGAGCACACTGCCGCCTACGA
CGGCATGATCGCCAACTACTTCGGTACCATGGTGCCCTCTTATGGCGACAACAAGGAGGGTGATGCCGAGTCTCGCTTCC
CGCGCACCTTCAACAGCCAGTTCATCAAGAAGCAGGACATGCGCTACGGCGAGAACAGCCATCAGGCTGCCGCCTTCTAT
GCCGAGGAGCATGCCACCCCGGGTTCTGTCTCCAGCGCCATCCAGCTGCAGGGCAAGGCGCTCTCCTACAACAACATCGC
CGATACCGACGCGGCGCTGGAGTGCGTGAAAGAGTTCAGTGAGCCCGCCTGCGTCATCGTCAAGCACGCCAACCCCTGTG
GCGTGGCCATCGGCGATGACCTGCTGACTGCCTACGATCGCGCCTACCAGACCGACCCCACTTCCGCCTTTGGCGGCATC
ATCGCCTTCAACCGCGAACTGGACGGGGCTACCGCAGCCGCCATAGTCGCGCGCCAGTTCGTGGAAGTGATCATTGCCCC
GAGCGTGAGCCAGGAAGCTCGCGATGTAGTCGCCGCCAAGCAGAACGTTCGTCTGCTCGAGTGCGGCCAGTGGACTGCAC
CGGCCCAGGGGTTTGACCTCAAACGCGTCAACGGCGGCCTGCTGATACAAGAGCGGGATTTCGGCATGGTGACGCAGGGT
GACCTGAAAGTGGTCAGCAAGCGCCAGCCAACCGAAGCCGAACTGAAAGATCTGCTGTTCTGCTGGAAAGTGGCCAAGTT
CGTCAAATCCAACGCCATCGTCTACGCCAAAGAGGGCCAGACCATAGGCGTCGGCGCCGGCCAGATGAGCCGGGTCTACT
CCGCCAAGATCGCCGGCATCAAAGCCGAGGATGAAGGGCTGACAGTAGCGGGCTCCGTCATGGCGTCCGATGCCTTCTTC
CCGTTCCGTGACGGTATCGATGCCGCTGCGGCCGCCGGTATCTCCTGCGTCATCCAGCCAGGTGGCTCGATGCGCGATCA
GGAAGTGATCGACGCCGCCGACGAACATGGCATGACAATGGTGTTCACCAACATGCGCCATTTCCGTCACTGA

Upstream 100 bases:

>100_bases
AAATCCCCCACTTACATTTATTTGGGGACTCTCTGTGTTTGGTGCGACCCAGTGCGCTTGCCTGCCATAGAAGTTCCCGC
ATGCTGAGGACGATAGTGTG

Downstream 100 bases:

>100_bases
TGAGTCAGGGTCGCCGCTGGCGACCCTTTTGTTGTCTCTTTTCAACTGACTATTTTCAACCGGCGACGCGCCATCTGCGA
GCCGAGCCGGTTACCACAAA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MEQARPIRRALLSVSDKTGILEFAKALNERGVALLSTGGTAKLLGDAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRDQDDAIMAQHNIYPIDMVVVNLYPFAATVAKAGCSLADAVENIDIGGPTMVRSAAKNHKDVAIVVKAADYARVIAEM
DANGNSLKLATRFDLAIAAFEHTAAYDGMIANYFGTMVPSYGDNKEGDAESRFPRTFNSQFIKKQDMRYGENSHQAAAFY
AEEHATPGSVSSAIQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDDLLTAYDRAYQTDPTSAFGGI
IAFNRELDGATAAAIVARQFVEVIIAPSVSQEARDVVAAKQNVRLLECGQWTAPAQGFDLKRVNGGLLIQERDFGMVTQG
DLKVVSKRQPTEAELKDLLFCWKVAKFVKSNAIVYAKEGQTIGVGAGQMSRVYSAKIAGIKAEDEGLTVAGSVMASDAFF
PFRDGIDAAAAAGISCVIQPGGSMRDQEVIDAADEHGMTMVFTNMRHFRH

Sequences:

>Translated_530_residues
MEQARPIRRALLSVSDKTGILEFAKALNERGVALLSTGGTAKLLGDAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRDQDDAIMAQHNIYPIDMVVVNLYPFAATVAKAGCSLADAVENIDIGGPTMVRSAAKNHKDVAIVVKAADYARVIAEM
DANGNSLKLATRFDLAIAAFEHTAAYDGMIANYFGTMVPSYGDNKEGDAESRFPRTFNSQFIKKQDMRYGENSHQAAAFY
AEEHATPGSVSSAIQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDDLLTAYDRAYQTDPTSAFGGI
IAFNRELDGATAAAIVARQFVEVIIAPSVSQEARDVVAAKQNVRLLECGQWTAPAQGFDLKRVNGGLLIQERDFGMVTQG
DLKVVSKRQPTEAELKDLLFCWKVAKFVKSNAIVYAKEGQTIGVGAGQMSRVYSAKIAGIKAEDEGLTVAGSVMASDAFF
PFRDGIDAAAAAGISCVIQPGGSMRDQEVIDAADEHGMTMVFTNMRHFRH
>Mature_530_residues
MEQARPIRRALLSVSDKTGILEFAKALNERGVALLSTGGTAKLLGDAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRDQDDAIMAQHNIYPIDMVVVNLYPFAATVAKAGCSLADAVENIDIGGPTMVRSAAKNHKDVAIVVKAADYARVIAEM
DANGNSLKLATRFDLAIAAFEHTAAYDGMIANYFGTMVPSYGDNKEGDAESRFPRTFNSQFIKKQDMRYGENSHQAAAFY
AEEHATPGSVSSAIQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDDLLTAYDRAYQTDPTSAFGGI
IAFNRELDGATAAAIVARQFVEVIIAPSVSQEARDVVAAKQNVRLLECGQWTAPAQGFDLKRVNGGLLIQERDFGMVTQG
DLKVVSKRQPTEAELKDLLFCWKVAKFVKSNAIVYAKEGQTIGVGAGQMSRVYSAKIAGIKAEDEGLTVAGSVMASDAFF
PFRDGIDAAAAAGISCVIQPGGSMRDQEVIDAADEHGMTMVFTNMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=39.344262295082, Blast_Score=282, Evalue=5e-76,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=78.5310734463277, Blast_Score=859, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=510, Percent_Identity=34.9019607843137, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=340, Percent_Identity=27.9411764705882, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=131, Percent_Identity=35.1145038167939, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=487, Percent_Identity=36.3449691991786, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6323056, Length=491, Percent_Identity=36.6598778004073, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24649832, Length=613, Percent_Identity=36.5415986949429, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56834; Mature: 56834

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQARPIRRALLSVSDKTGILEFAKALNERGVALLSTGGTAKLLGDAGLPVTEVSDYTGF
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRDQDDAIMAQHNIYPIDMVVVNLYPFAATVAKAGCSLA
HHHHCCCEEEECCHHHCCCCCCCCCCCCEEECCCCCEEEEEEEEHHHHHHHHHHHCCHHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKAADYARVIAEMDANGNSLKLATRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEEECCEEEEHH
EHTAAYDGMIANYFGTMVPSYGDNKEGDAESRFPRTFNSQFIKKQDMRYGENSHQAAAFY
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
AEEHATPGSVSSAIQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDD
HHHCCCCCCCCCEEEECCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECHH
LLTAYDRAYQTDPTSAFGGIIAFNRELDGATAAAIVARQFVEVIIAPSVSQEARDVVAAK
HHHHHHHHHCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QNVRLLECGQWTAPAQGFDLKRVNGGLLIQERDFGMVTQGDLKVVSKRQPTEAELKDLLF
CCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCEECCCHHEECCCCCCHHHHHHHHH
CWKVAKFVKSNAIVYAKEGQTIGVGAGQMSRVYSAKIAGIKAEDEGLTVAGSVMASDAFF
HHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCCEEECHHHHCCCCC
PFRDGIDAAAAAGISCVIQPGGSMRDQEVIDAADEHGMTMVFTNMRHFRH
CCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECHHHHCC
>Mature Secondary Structure
MEQARPIRRALLSVSDKTGILEFAKALNERGVALLSTGGTAKLLGDAGLPVTEVSDYTGF
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRDQDDAIMAQHNIYPIDMVVVNLYPFAATVAKAGCSLA
HHHHCCCEEEECCHHHCCCCCCCCCCCCEEECCCCCEEEEEEEEHHHHHHHHHHHCCHHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKAADYARVIAEMDANGNSLKLATRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEEECCEEEEHH
EHTAAYDGMIANYFGTMVPSYGDNKEGDAESRFPRTFNSQFIKKQDMRYGENSHQAAAFY
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
AEEHATPGSVSSAIQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDD
HHHCCCCCCCCCEEEECCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECHH
LLTAYDRAYQTDPTSAFGGIIAFNRELDGATAAAIVARQFVEVIIAPSVSQEARDVVAAK
HHHHHHHHHCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QNVRLLECGQWTAPAQGFDLKRVNGGLLIQERDFGMVTQGDLKVVSKRQPTEAELKDLLF
CCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCEECCCHHEECCCCCCHHHHHHHHH
CWKVAKFVKSNAIVYAKEGQTIGVGAGQMSRVYSAKIAGIKAEDEGLTVAGSVMASDAFF
HHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCCEEECHHHHCCCCC
PFRDGIDAAAAAGISCVIQPGGSMRDQEVIDAADEHGMTMVFTNMRHFRH
CCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA