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The map label for this gene is purD [H]

Identifier: 145300328

GI number: 145300328

Start: 3740490

End: 3741779

Strand: Direct

Name: purD [H]

Synonym: ASA_3452

Alternate gene names: 145300328

Gene position: 3740490-3741779 (Clockwise)

Preceding gene: 145300327

Following gene: 145300329

Centisome position: 79.54

GC content: 62.48

Gene sequence:

>1290_bases
ATGAAAGTACTGATCATTGGCAACGGCGGTCGTGAACACGCCCTGGCCTGGAAGGCCAAACACTCTCCACTGGTGCACCA
CGTCTTCGTCGCACCGGGCAATGCGGGCACCCAGCTGGAGCCTGGCGTCGAGAACGTCGCCATCGAGGCGACCGACATCG
AAGGGCTGCTGGCCTTTGCCAAAGAGAAACAGGTCGGTCTCACCATAGTCGGACCTGAAGCCCCGCTGGTGAAGGGGGTG
GTCGATGCCTTCCGCGCCGCCGGTCAGCCTATCTTTGGCCCAACCGCCGCCGCCGCCCAGCTGGAAGGCTCCAAGGCCTT
CACCAAGGATTTCCTGGCCCGTCACGCCATTCCGACCGCCGACTACCAGAACTTCACCGAGGTGGAGCCTGCGCTCGCCT
ATCTGCGTGAGAAGGGTGCTCCCATCGTCATCAAGGCCGATGGTCTGGCGGCAGGCAAAGGCGTGATCGTCGCCATGACC
TTGCAAGAAGCGGAAGACGCCGTGCGTGACATGCTCTCCGGCAATGCATTCGGCGATGCCGGTGCCCGTGTGGTGATCGA
AGAGTTTCTGGACGGGGAAGAAGCAAGCTTCATCGTCATGGTGGATGGCGAGCATGTGCTGCCCATGGCCACCAGTCAGG
ATCACAAGCGGGTCGGCGATGGCGACACAGGTCCCAATACCGGCGGCATGGGCGCTTACTCCCCCGCACCCGTGGTCACC
GATGCGGTGCACAACAAGGTGATGGAGCAGGTGATCATGCCAACCGTGCGTGGCATGGCGGCCGAAGGCAATGTCTACAC
CGGCTTCCTCTACGCCGGCTTGATGATCGATGGCCAGGGCAACCCCAAGGTGATCGAGTTCAACTGCCGCTTCGGCGACC
CCGAGACCCAGCCCATCATGCTGCGGATGCAGTCCGATCTGGTGGAACTCTGCCTAGCAGCCTGCGAAGGCAAGCTGGAT
CAGAAAGAGGCCATCTATGATCCTCGCGCCGCCATCGGTGTTGTGCTGGCAGCGGGTGGTTATCCTGGCGACTACAGCAG
CAATCACCCCATCAGCGGCTTGCCGGTGGATGAAGTGGCCGGTGAGAAGGTGTTCCATGCCGGGACCCGACTGGAAGGGG
ATACCAGCCTGACCCAGGGTGGCCGCGTGCTGTGCGCCACCGCGCTGGGCTACACGGTTGCCGAGGCGCAGCAACGCGCC
TATCAGCTTGCTGGTCGCATCAAGTGGGATGGCATCTTCTACCGTAAGGACATCGGCTGGCGTGCCATAGAGCGCGAGCA
GGAGCAGTAA

Upstream 100 bases:

>100_bases
TGGCGACCCTTTTGTTGTCTCTTTTCAACTGACTATTTTCAACCGGCGACGCGCCATCTGCGAGCCGAGCCGGTTACCAC
AAATAGAAGCGAGCGATACG

Downstream 100 bases:

>100_bases
TTCCCGCCTTCGCCACCATCATGATCATCAGGGCCTGCGGGCCCTTTTTTCTGCCCGGCACAGCCCGACTACCAGCAACG
GGGTTGTGATGTCGCTATTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKVLIIGNGGREHALAWKAKHSPLVHHVFVAPGNAGTQLEPGVENVAIEATDIEGLLAFAKEKQVGLTIVGPEAPLVKGV
VDAFRAAGQPIFGPTAAAAQLEGSKAFTKDFLARHAIPTADYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DAVHNKVMEQVIMPTVRGMAAEGNVYTGFLYAGLMIDGQGNPKVIEFNCRFGDPETQPIMLRMQSDLVELCLAACEGKLD
QKEAIYDPRAAIGVVLAAGGYPGDYSSNHPISGLPVDEVAGEKVFHAGTRLEGDTSLTQGGRVLCATALGYTVAEAQQRA
YQLAGRIKWDGIFYRKDIGWRAIEREQEQ

Sequences:

>Translated_429_residues
MKVLIIGNGGREHALAWKAKHSPLVHHVFVAPGNAGTQLEPGVENVAIEATDIEGLLAFAKEKQVGLTIVGPEAPLVKGV
VDAFRAAGQPIFGPTAAAAQLEGSKAFTKDFLARHAIPTADYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DAVHNKVMEQVIMPTVRGMAAEGNVYTGFLYAGLMIDGQGNPKVIEFNCRFGDPETQPIMLRMQSDLVELCLAACEGKLD
QKEAIYDPRAAIGVVLAAGGYPGDYSSNHPISGLPVDEVAGEKVFHAGTRLEGDTSLTQGGRVLCATALGYTVAEAQQRA
YQLAGRIKWDGIFYRKDIGWRAIEREQEQ
>Mature_429_residues
MKVLIIGNGGREHALAWKAKHSPLVHHVFVAPGNAGTQLEPGVENVAIEATDIEGLLAFAKEKQVGLTIVGPEAPLVKGV
VDAFRAAGQPIFGPTAAAAQLEGSKAFTKDFLARHAIPTADYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DAVHNKVMEQVIMPTVRGMAAEGNVYTGFLYAGLMIDGQGNPKVIEFNCRFGDPETQPIMLRMQSDLVELCLAACEGKLD
QKEAIYDPRAAIGVVLAAGGYPGDYSSNHPISGLPVDEVAGEKVFHAGTRLEGDTSLTQGGRVLCATALGYTVAEAQQRA
YQLAGRIKWDGIFYRKDIGWRAIEREQEQ

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=49.4172494172494, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=49.4172494172494, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=49.4172494172494, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=49.4172494172494, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=80.8411214953271, Blast_Score=702, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=40.1408450704225, Blast_Score=301, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=41.9282511210762, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.2436194895592, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=45.2436194895592, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45538; Mature: 45538

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIIGNGGREHALAWKAKHSPLVHHVFVAPGNAGTQLEPGVENVAIEATDIEGLLAFA
CEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHH
KEKQVGLTIVGPEAPLVKGVVDAFRAAGQPIFGPTAAAAQLEGSKAFTKDFLARHAIPTA
HCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCC
DYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLQEAEDAVRDMLSGNAFGDA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
DAVHNKVMEQVIMPTVRGMAAEGNVYTGFLYAGLMIDGQGNPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCEEE
LRMQSDLVELCLAACEGKLDQKEAIYDPRAAIGVVLAAGGYPGDYSSNHPISGLPVDEVA
EEEHHHHHHHHHHHHCCCCCCHHHHCCCCHHEEEEEECCCCCCCCCCCCCCCCCCHHHHC
GEKVFHAGTRLEGDTSLTQGGRVLCATALGYTVAEAQQRAYQLAGRIKWDGIFYRKDIGW
CCHHEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCEEEECEEEEECCCCC
RAIEREQEQ
HHHHHCCCC
>Mature Secondary Structure
MKVLIIGNGGREHALAWKAKHSPLVHHVFVAPGNAGTQLEPGVENVAIEATDIEGLLAFA
CEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHH
KEKQVGLTIVGPEAPLVKGVVDAFRAAGQPIFGPTAAAAQLEGSKAFTKDFLARHAIPTA
HCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCC
DYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLQEAEDAVRDMLSGNAFGDA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GARVVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
DAVHNKVMEQVIMPTVRGMAAEGNVYTGFLYAGLMIDGQGNPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCEEE
LRMQSDLVELCLAACEGKLDQKEAIYDPRAAIGVVLAAGGYPGDYSSNHPISGLPVDEVA
EEEHHHHHHHHHHHHCCCCCCHHHHCCCCHHEEEEEECCCCCCCCCCCCCCCCCCHHHHC
GEKVFHAGTRLEGDTSLTQGGRVLCATALGYTVAEAQQRAYQLAGRIKWDGIFYRKDIGW
CCHHEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCEEEECEEEEECCCCC
RAIEREQEQ
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]