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The map label for this gene is hemN [H]

Identifier: 145300323

GI number: 145300323

Start: 3735547

End: 3736692

Strand: Direct

Name: hemN [H]

Synonym: ASA_3447

Alternate gene names: 145300323

Gene position: 3735547-3736692 (Clockwise)

Preceding gene: 145300322

Following gene: 145300327

Centisome position: 79.44

GC content: 62.13

Gene sequence:

>1146_bases
ATGCTGCAACTGCCGCCCCTCTCCCTCTATATCCATGTGCCCTGGTGCGTCCAGAAGTGCCCCTACTGCGACTTCAACTC
CCACGCCCAGAAAGGGGAGCTGCCCCACCTCGAGTATGTGGCCGCTCTGCTGGAGGATCTGGATCTGGATCTGAAGTGGG
CCCAGGGGCGTCCGCTCTCCTCCATTTTTATCGGCGGTGGCACCCCCAGCCTGCTGACGGTAGAGGCCATGCAGACCCTG
CTGGACGGGGTGCGTGCTCGCATCCCCCTGACGCCGGATTGCGAGATCACCATGGAGGCCAACCCGGGCACTGTGGAGGC
TGACAAGTTCGCAGGCTTCAAGCAGGCAGGCATCAACCGCATCTCCATCGGGGTGCAGAGCTTCCAGCAAGAAAAATTGA
CCCGGCTCGGTCGCATCCACGGCCCCGAAGAGGCCCGCAAGGCCGCTGCGCTGGCCCACGCCATCGCGCTTCCCACCTTC
AACCTGGACCTGATGCACGGCCTGCCGGATCAGAGCCTGGACGACGCCCTCTATGATCTGCAACAGGCCATCGACTGCGC
GCCGCCGCATCTCTCCTGGTATCAGCTCACCATAGAGCCGAACACGGCGTTCGCCTCCAAGCCACCACGGCTGCCGGAGG
ATGACACCCTGTGGGACATCTACGAGCAGGGCCATGCCATGCTCACCGCCCGGGGCTATCAGCAGTACGAGATCTCGGCC
TATGCCAAAGCGGGCTATCAGTGCCGCCACAACCTCAACTACTGGCGCTTCGGCGATTATCTCGGGATAGGTTGCGGCGC
CCACGGCAAGATGACGGACCTTGCTAGCGGCCAGATACTGCGCAGCGCCAAGGTGAAGCACCCGAAAGGCTATCTGGATC
CCGCCCGCCCCTACCTGGACAAGCAGTGGCAGGTGACCGAGGATGAACTGCCGCTGGAGTATTTCATGAATCGGTTCAGA
TTATTCGAGCCGGCCCCCAAGGCGGAGTTCGAGGCCTATACCGGCTTGGGCCTGGAGCGGGTGGAGACTATGCTCGCGAT
TGCCCAGACCAAGGAGCTGATCGAAGATCTGGGTGACTGCTGGCAGCTCACCGCCCTCGGCCATCGTTACCTGAATGTGC
TGCTGGAACTCTTTATGGACGAGTAA

Upstream 100 bases:

>100_bases
GCTGCGACTCACTGAGTTCGCCGCCTCTCGCCAATCAAGCATTGTCATCTACAATGGGGGCCGTCAGCCCCCGTTTTTAT
TTTTGATTATTGGAAGCCCC

Downstream 100 bases:

>100_bases
AAACTTGTTCACGATCTTGCTGCGAAACCGTGATCAAGGCTCATGCGCTGCTCGGAATCCTCACGTATGCCTATACGCTC
CGGTTCCTGCGCTGCTCTTC

Product: oxygen-independent coproporphyrinogen III oxidase

Products: Protoporphyrinogen IX; CO2; H2O

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLQLPPLSLYIHVPWCVQKCPYCDFNSHAQKGELPHLEYVAALLEDLDLDLKWAQGRPLSSIFIGGGTPSLLTVEAMQTL
LDGVRARIPLTPDCEITMEANPGTVEADKFAGFKQAGINRISIGVQSFQQEKLTRLGRIHGPEEARKAAALAHAIALPTF
NLDLMHGLPDQSLDDALYDLQQAIDCAPPHLSWYQLTIEPNTAFASKPPRLPEDDTLWDIYEQGHAMLTARGYQQYEISA
YAKAGYQCRHNLNYWRFGDYLGIGCGAHGKMTDLASGQILRSAKVKHPKGYLDPARPYLDKQWQVTEDELPLEYFMNRFR
LFEPAPKAEFEAYTGLGLERVETMLAIAQTKELIEDLGDCWQLTALGHRYLNVLLELFMDE

Sequences:

>Translated_381_residues
MLQLPPLSLYIHVPWCVQKCPYCDFNSHAQKGELPHLEYVAALLEDLDLDLKWAQGRPLSSIFIGGGTPSLLTVEAMQTL
LDGVRARIPLTPDCEITMEANPGTVEADKFAGFKQAGINRISIGVQSFQQEKLTRLGRIHGPEEARKAAALAHAIALPTF
NLDLMHGLPDQSLDDALYDLQQAIDCAPPHLSWYQLTIEPNTAFASKPPRLPEDDTLWDIYEQGHAMLTARGYQQYEISA
YAKAGYQCRHNLNYWRFGDYLGIGCGAHGKMTDLASGQILRSAKVKHPKGYLDPARPYLDKQWQVTEDELPLEYFMNRFR
LFEPAPKAEFEAYTGLGLERVETMLAIAQTKELIEDLGDCWQLTALGHRYLNVLLELFMDE
>Mature_381_residues
MLQLPPLSLYIHVPWCVQKCPYCDFNSHAQKGELPHLEYVAALLEDLDLDLKWAQGRPLSSIFIGGGTPSLLTVEAMQTL
LDGVRARIPLTPDCEITMEANPGTVEADKFAGFKQAGINRISIGVQSFQQEKLTRLGRIHGPEEARKAAALAHAIALPTF
NLDLMHGLPDQSLDDALYDLQQAIDCAPPHLSWYQLTIEPNTAFASKPPRLPEDDTLWDIYEQGHAMLTARGYQQYEISA
YAKAGYQCRHNLNYWRFGDYLGIGCGAHGKMTDLASGQILRSAKVKHPKGYLDPARPYLDKQWQVTEDELPLEYFMNRFR
LFEPAPKAEFEAYTGLGLERVETMLAIAQTKELIEDLGDCWQLTALGHRYLNVLLELFMDE

Specific function: Unknown

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=283, Percent_Identity=34.982332155477, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1789325, Length=381, Percent_Identity=65.8792650918635, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI87082341, Length=284, Percent_Identity=28.8732394366197, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: 1.3.3.3

Molecular weight: Translated: 42893; Mature: 42893

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLPPLSLYIHVPWCVQKCPYCDFNSHAQKGELPHLEYVAALLEDLDLDLKWAQGRPLS
CCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCC
SIFIGGGTPSLLTVEAMQTLLDGVRARIPLTPDCEITMEANPGTVEADKFAGFKQAGINR
EEEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCHHHHCCCE
ISIGVQSFQQEKLTRLGRIHGPEEARKAAALAHAIALPTFNLDLMHGLPDQSLDDALYDL
EEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHH
QQAIDCAPPHLSWYQLTIEPNTAFASKPPRLPEDDTLWDIYEQGHAMLTARGYQQYEISA
HHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEHHH
YAKAGYQCRHNLNYWRFGDYLGIGCGAHGKMTDLASGQILRSAKVKHPKGYLDPARPYLD
HHHCCHHHHCCCCEEEECCEEEECCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCC
KQWQVTEDELPLEYFMNRFRLFEPAPKAEFEAYTGLGLERVETMLAIAQTKELIEDLGDC
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
WQLTALGHRYLNVLLELFMDE
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQLPPLSLYIHVPWCVQKCPYCDFNSHAQKGELPHLEYVAALLEDLDLDLKWAQGRPLS
CCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCC
SIFIGGGTPSLLTVEAMQTLLDGVRARIPLTPDCEITMEANPGTVEADKFAGFKQAGINR
EEEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCHHHHCCCE
ISIGVQSFQQEKLTRLGRIHGPEEARKAAALAHAIALPTFNLDLMHGLPDQSLDDALYDL
EEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHH
QQAIDCAPPHLSWYQLTIEPNTAFASKPPRLPEDDTLWDIYEQGHAMLTARGYQQYEISA
HHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEHHH
YAKAGYQCRHNLNYWRFGDYLGIGCGAHGKMTDLASGQILRSAKVKHPKGYLDPARPYLD
HHHCCHHHHCCCCEEEECCEEEECCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCC
KQWQVTEDELPLEYFMNRFRLFEPAPKAEFEAYTGLGLERVETMLAIAQTKELIEDLGDC
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
WQLTALGHRYLNVLLELFMDE
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen III; O2; H+

Specific reaction: coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]