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The map label for this gene is dcuA [H]

Identifier: 145300314

GI number: 145300314

Start: 3723221

End: 3724540

Strand: Direct

Name: dcuA [H]

Synonym: ASA_3436

Alternate gene names: 145300314

Gene position: 3723221-3724540 (Clockwise)

Preceding gene: 145300313

Following gene: 145300315

Centisome position: 79.18

GC content: 60.15

Gene sequence:

>1320_bases
ATGATTGTTATCGAGTTACTTGTCGTGCTCGCCGCTATCTATCTGGGTGCCCGGATAGGCAGTATCGGTATCGGCTTTGC
CGGTGGTCTGGGTGTGCTGGTGCTGACCTGGGGCCTGGGGGTACAAATTCCGAGCGACCAACTGGTCACCTTCATCCCCT
GGGACGTGATCATGATCATCGCGTCCGTCATCTGTGCCATTGCCTGTATGCAACTGGCCGGTGGCATGGACTACCTGGTT
TCGCTGGCAGAAAAGGCTCTGCGCAAGAATCCCAAGTACATCACCTTTGCCGCCCCCATCGTCACCTATCTGATGACAAT
GCTGGCCGGTACCGGTCACACCGCCTTCTCCACCCTGCCGGTAATCGCCGAAGTGGCCAAGGAGCAGGGCATTCGTCCTT
CCCGTCCGCTCTCCATCGCGGTCGTCGCCTCTCAGATTGCCATCACCGCCTCCCCGATCTCCGCGGCCGTGGTTGCCTTC
TCCGGCATGCTTGAGCCGTTCGGTGTCGACTACCTGACCCTGCTGGCCATCTGCATTCCGTCCACCTTCATCGCCTGTAT
CCTGGGCGCCTTCATTGCCAACATGATGGGCAAACCGCTGGATCAGGATGAAGTCTATCTGGAGCGTAAAGCCAAAGGGC
TGATCAAGCTGCGCGGCACCACCAAGCTGGAGCTGAAGCCTTACGCCAAGGTTTCTGTCTGGATCTTCATCGCCGCCATC
GTGGCCGTCATGGCTTATGCCACCGCCATCTCAGGCAAAGTGGGCCTGATCGCCAATCCGCCAATTCCCCGTGACGGTGC
CATCATGGGCATCATGCTGGCTGCTGCCACCATCATGACCCTGGTCTGCAAACTGGACGCCACTCAAATCACCAACATGC
CGACCTTCAAGTCCGGTATGTCCGCCTGTATCTGTGTACTGGGTGTGGCCTGGCTGGGCAACGTCTTCGTGAAAGGCAAC
ATGGACACCATTCAGCTGATCGCTGGCGACCTGCTGAACCAGTATTCCTGGCTGCTGGCCGTGGTGCTGTTCTTCTCCGC
CATGCTGCTCTACTCACAAGGCGCCACCACCAAGGCCCTGATGCCGGCCGCACTGGCGCTGGGTGTCAGCCCGCTGACCG
CCATCGCCTCCTTCGCCGCCGTGAGCGCCCTGTTCGTGCTGCCGACCTACCCGACCCTGCTGGCCGCAGTCGAGATGGAC
GACACCGGCTCGACCCGGATCGGCAAGGCCGTGTTCAACCACCCCTTCTTCATCCCCGGCACCGCGACCATCGCTTTCGC
CGTGCTACTGGGCTTCTTCTTTGGCGGTCTGATGCTCTAA

Upstream 100 bases:

>100_bases
GCCCATAAAGTCAGTTCCCGATCGGTCTCACCCAGTCCCATCGGGAATTGGCTTTTCTGCCAAATAAAACCTAAAAAAAC
TGGAAAATGGAGTGTTCGCT

Downstream 100 bases:

>100_bases
TCAGCATTTAATGCCATAAAAACTCAAACGGCCCTCATTGGGCCGTTTTTTATTCCTCGTTTTTCCCTTTCTCCGCCAAT
TAATCCTCGACCGCTTCAGT

Product: anaerobic c4-dicarboxylate membrane transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIVIELLVVLAAIYLGARIGSIGIGFAGGLGVLVLTWGLGVQIPSDQLVTFIPWDVIMIIASVICAIACMQLAGGMDYLV
SLAEKALRKNPKYITFAAPIVTYLMTMLAGTGHTAFSTLPVIAEVAKEQGIRPSRPLSIAVVASQIAITASPISAAVVAF
SGMLEPFGVDYLTLLAICIPSTFIACILGAFIANMMGKPLDQDEVYLERKAKGLIKLRGTTKLELKPYAKVSVWIFIAAI
VAVMAYATAISGKVGLIANPPIPRDGAIMGIMLAAATIMTLVCKLDATQITNMPTFKSGMSACICVLGVAWLGNVFVKGN
MDTIQLIAGDLLNQYSWLLAVVLFFSAMLLYSQGATTKALMPAALALGVSPLTAIASFAAVSALFVLPTYPTLLAAVEMD
DTGSTRIGKAVFNHPFFIPGTATIAFAVLLGFFFGGLML

Sequences:

>Translated_439_residues
MIVIELLVVLAAIYLGARIGSIGIGFAGGLGVLVLTWGLGVQIPSDQLVTFIPWDVIMIIASVICAIACMQLAGGMDYLV
SLAEKALRKNPKYITFAAPIVTYLMTMLAGTGHTAFSTLPVIAEVAKEQGIRPSRPLSIAVVASQIAITASPISAAVVAF
SGMLEPFGVDYLTLLAICIPSTFIACILGAFIANMMGKPLDQDEVYLERKAKGLIKLRGTTKLELKPYAKVSVWIFIAAI
VAVMAYATAISGKVGLIANPPIPRDGAIMGIMLAAATIMTLVCKLDATQITNMPTFKSGMSACICVLGVAWLGNVFVKGN
MDTIQLIAGDLLNQYSWLLAVVLFFSAMLLYSQGATTKALMPAALALGVSPLTAIASFAAVSALFVLPTYPTLLAAVEMD
DTGSTRIGKAVFNHPFFIPGTATIAFAVLLGFFFGGLML
>Mature_439_residues
MIVIELLVVLAAIYLGARIGSIGIGFAGGLGVLVLTWGLGVQIPSDQLVTFIPWDVIMIIASVICAIACMQLAGGMDYLV
SLAEKALRKNPKYITFAAPIVTYLMTMLAGTGHTAFSTLPVIAEVAKEQGIRPSRPLSIAVVASQIAITASPISAAVVAF
SGMLEPFGVDYLTLLAICIPSTFIACILGAFIANMMGKPLDQDEVYLERKAKGLIKLRGTTKLELKPYAKVSVWIFIAAI
VAVMAYATAISGKVGLIANPPIPRDGAIMGIMLAAATIMTLVCKLDATQITNMPTFKSGMSACICVLGVAWLGNVFVKGN
MDTIQLIAGDLLNQYSWLLAVVLFFSAMLLYSQGATTKALMPAALALGVSPLTAIASFAAVSALFVLPTYPTLLAAVEMD
DTGSTRIGKAVFNHPFFIPGTATIAFAVLLGFFFGGLML

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=440, Percent_Identity=58.8636363636364, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1790565, Length=396, Percent_Identity=35.3535353535354, Blast_Score=228, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46115; Mature: 46115

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIELLVVLAAIYLGARIGSIGIGFAGGLGVLVLTWGLGVQIPSDQLVTFIPWDVIMII
CHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHH
ASVICAIACMQLAGGMDYLVSLAEKALRKNPKYITFAAPIVTYLMTMLAGTGHTAFSTLP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHH
VIAEVAKEQGIRPSRPLSIAVVASQIAITASPISAAVVAFSGMLEPFGVDYLTLLAICIP
HHHHHHHHCCCCCCCCEEEEEEHHHHHEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
STFIACILGAFIANMMGKPLDQDEVYLERKAKGLIKLRGTTKLELKPYAKVSVWIFIAAI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCEEEEECCCHHHHHHHHHHHH
VAVMAYATAISGKVGLIANPPIPRDGAIMGIMLAAATIMTLVCKLDATQITNMPTFKSGM
HHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHH
SACICVLGVAWLGNVFVKGNMDTIQLIAGDLLNQYSWLLAVVLFFSAMLLYSQGATTKAL
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
MPAALALGVSPLTAIASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKAVFNHPFFIPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHCCCCCCCC
TATIAFAVLLGFFFGGLML
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVIELLVVLAAIYLGARIGSIGIGFAGGLGVLVLTWGLGVQIPSDQLVTFIPWDVIMII
CHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHH
ASVICAIACMQLAGGMDYLVSLAEKALRKNPKYITFAAPIVTYLMTMLAGTGHTAFSTLP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHH
VIAEVAKEQGIRPSRPLSIAVVASQIAITASPISAAVVAFSGMLEPFGVDYLTLLAICIP
HHHHHHHHCCCCCCCCEEEEEEHHHHHEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
STFIACILGAFIANMMGKPLDQDEVYLERKAKGLIKLRGTTKLELKPYAKVSVWIFIAAI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCEEEEECCCHHHHHHHHHHHH
VAVMAYATAISGKVGLIANPPIPRDGAIMGIMLAAATIMTLVCKLDATQITNMPTFKSGM
HHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHH
SACICVLGVAWLGNVFVKGNMDTIQLIAGDLLNQYSWLLAVVLFFSAMLLYSQGATTKAL
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
MPAALALGVSPLTAIASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKAVFNHPFFIPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHCCCCCCCC
TATIAFAVLLGFFFGGLML
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]