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The map label for this gene is aspA [H]

Identifier: 145300313

GI number: 145300313

Start: 3721651

End: 3723093

Strand: Direct

Name: aspA [H]

Synonym: ASA_3435

Alternate gene names: 145300313

Gene position: 3721651-3723093 (Clockwise)

Preceding gene: 145300311

Following gene: 145300314

Centisome position: 79.14

GC content: 57.31

Gene sequence:

>1443_bases
ATGAGCGACATCAAGAACGTCCGCATTGAAGAAGACCTGCTGGGCACCAAAGAAGTTTCCAATGAGCTCTACTACGGCGT
GCATACCCAGCGCGCAGTCGAAAACTTCAAGCTGAGCACTCAGAAGATTTCTGACGTTCCGGAATTTGTCCGCGGCATGG
TGCTGACCAAGAAGGCCGCCGCCCTGGCGAACGGCGAACTGGGCACCATCCGTCCTGAAATTGCTGACAAGATCGTAGAA
GCCTGCGACCTGATGCTGAACACCGGCAAATGTTTCGACCAGTTCCCGGTCGACGTTTACCAGGGTGGTGCAGGCACCTC
CGTCAACATGAACACCAACGAAGTACTGGCCAACATCGCCCTCGAGATCATGGGTCTGGAGAAGGGCCGTTACGACGTCA
TCAACCCCAATGACCACGTCAACAAGTGCCAATCCACCAACGACGCCTACCCTACCGGCTTCCGCGTTGCCGTGGTAAAC
AGCACAGACACCACACTGCATGCCCTGGAAGCACTGATCGCTGCCTTCGATGCCAAGGCCAAGGAATTCAAAAGCATCCT
GAAGATGGGCCGTACCCAGCTGCAGGACGCGGTTCCCATGACACTGGGTCAGGAGTTCCACGCCTTCGCCGTTACCCTGC
GTGAAGAGATCAAGTCCATCAAGCGTTGCCAGGAGCTGCTGCTGGAAGTGAACCTGGGGGCAACCGCCATCGGTACCGGC
CTGAACACCCCGCCCGAGTACTCTGCGCTGGCCATCAAACGCCTGGCCGAGATCACGGGTCGTGCTTATGTTCCGGCAGA
AGACCTGATCGAAGCAACCTCCGACTGTGGCGCCTATGTGATGCTGCACGGTGCCATCAAGCGCATGGCCATGAAAATGT
CCAAGATCTGTAACGATCTGCGTCTGCTCTCCTCCGGTCCGCGCACTGCCCTCAAAGAGATCAACCTGCCGGAAATGCAG
GCCGGTTCCTCCATCATGCCGGCCAAGGTCAACCCGGTTATCCCGGAAGTGGTCAACCAGACCTGCTTCAAGGTATTCGG
TAACGATGTCACCATCACTTTCGCCGCCGAAGCCGGTCAGCTGCAGCTGAACGTCATGGAGCCGGTGATTGGTCAGGCCA
TGTTCGAATCCCTGAGCCTGATGGAAAAAGCCTGTATCTCCCTGCGCGAGAAGTGTGTGGAAGGTATTACCGCCAACCCA
GAGATCTGCATGAACCACGTGCTGAACTCCATCGGCATCGTGACCTACCTGAACCCCTTCATCGGCCACCACGAAGGGGA
TATCGTCGGCAAGATCTGCGCCCAGACCGGCAAGAACGTCCGTGAGGTCGTGCTGGAACGCGGCCTGCTGACCGCCGAAC
AGCTGGACGAAATCCTTTCCATCGAGAACCTGATGAACCCGCAATACAAAGCCAAGCGCTACACCCGCGAAGCCACCGTA
TAA

Upstream 100 bases:

>100_bases
CCCCACTCGATGTGTGGTGCTTCAGGATGAGCCGTGCGTAAGGAACTCGCGACCACTCGGTGAGGATCGTTTCCATTTAT
CTGATTTCTGAGGCATTACA

Downstream 100 bases:

>100_bases
GCGAGAAAATGGGCCGGGTTTATCCCGGCCCATAAAGTCAGTTCCCGATCGGTCTCACCCAGTCCCATCGGGAATTGGCT
TTTCTGCCAAATAAAACCTA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSDIKNVRIEEDLLGTKEVSNELYYGVHTQRAVENFKLSTQKISDVPEFVRGMVLTKKAAALANGELGTIRPEIADKIVE
ACDLMLNTGKCFDQFPVDVYQGGAGTSVNMNTNEVLANIALEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRVAVVN
STDTTLHALEALIAAFDAKAKEFKSILKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
LNTPPEYSALAIKRLAEITGRAYVPAEDLIEATSDCGAYVMLHGAIKRMAMKMSKICNDLRLLSSGPRTALKEINLPEMQ
AGSSIMPAKVNPVIPEVVNQTCFKVFGNDVTITFAAEAGQLQLNVMEPVIGQAMFESLSLMEKACISLREKCVEGITANP
EICMNHVLNSIGIVTYLNPFIGHHEGDIVGKICAQTGKNVREVVLERGLLTAEQLDEILSIENLMNPQYKAKRYTREATV

Sequences:

>Translated_480_residues
MSDIKNVRIEEDLLGTKEVSNELYYGVHTQRAVENFKLSTQKISDVPEFVRGMVLTKKAAALANGELGTIRPEIADKIVE
ACDLMLNTGKCFDQFPVDVYQGGAGTSVNMNTNEVLANIALEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRVAVVN
STDTTLHALEALIAAFDAKAKEFKSILKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
LNTPPEYSALAIKRLAEITGRAYVPAEDLIEATSDCGAYVMLHGAIKRMAMKMSKICNDLRLLSSGPRTALKEINLPEMQ
AGSSIMPAKVNPVIPEVVNQTCFKVFGNDVTITFAAEAGQLQLNVMEPVIGQAMFESLSLMEKACISLREKCVEGITANP
EICMNHVLNSIGIVTYLNPFIGHHEGDIVGKICAQTGKNVREVVLERGLLTAEQLDEILSIENLMNPQYKAKRYTREATV
>Mature_479_residues
SDIKNVRIEEDLLGTKEVSNELYYGVHTQRAVENFKLSTQKISDVPEFVRGMVLTKKAAALANGELGTIRPEIADKIVEA
CDLMLNTGKCFDQFPVDVYQGGAGTSVNMNTNEVLANIALEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRVAVVNS
TDTTLHALEALIAAFDAKAKEFKSILKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTGL
NTPPEYSALAIKRLAEITGRAYVPAEDLIEATSDCGAYVMLHGAIKRMAMKMSKICNDLRLLSSGPRTALKEINLPEMQA
GSSIMPAKVNPVIPEVVNQTCFKVFGNDVTITFAAEAGQLQLNVMEPVIGQAMFESLSLMEKACISLREKCVEGITANPE
ICMNHVLNSIGIVTYLNPFIGHHEGDIVGKICAQTGKNVREVVLERGLLTAEQLDEILSIENLMNPQYKAKRYTREATV

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=470, Percent_Identity=39.5744680851064, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI87082375, Length=473, Percent_Identity=74.4186046511628, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=472, Percent_Identity=38.771186440678, Blast_Score=318, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=38.4449244060475, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=37.6146788990826, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=38.5775862068966, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24662684, Length=472, Percent_Identity=38.135593220339, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI78710009, Length=470, Percent_Identity=37.6595744680851, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=37.3650107991361, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=37.3650107991361, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24583245, Length=468, Percent_Identity=32.6923076923077, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52545; Mature: 52414

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKNVRIEEDLLGTKEVSNELYYGVHTQRAVENFKLSTQKISDVPEFVRGMVLTKKAA
CCCCCCCCCHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALANGELGTIRPEIADKIVEACDLMLNTGKCFDQFPVDVYQGGAGTSVNMNTNEVLANIA
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCCCEECCCHHHHHHHHH
LEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRVAVVNSTDTTLHALEALIAAFDAKA
HHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
KEFKSILKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
LNTPPEYSALAIKRLAEITGRAYVPAEDLIEATSDCGAYVMLHGAIKRMAMKMSKICNDL
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTALKEINLPEMQAGSSIMPAKVNPVIPEVVNQTCFKVFGNDVTITFAAEAGQ
HHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCC
LQLNVMEPVIGQAMFESLSLMEKACISLREKCVEGITANPEICMNHVLNSIGIVTYLNPF
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGHHEGDIVGKICAQTGKNVREVVLERGLLTAEQLDEILSIENLMNPQYKAKRYTREATV
HCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SDIKNVRIEEDLLGTKEVSNELYYGVHTQRAVENFKLSTQKISDVPEFVRGMVLTKKAA
CCCCCCCCHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALANGELGTIRPEIADKIVEACDLMLNTGKCFDQFPVDVYQGGAGTSVNMNTNEVLANIA
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCCCEECCCHHHHHHHHH
LEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRVAVVNSTDTTLHALEALIAAFDAKA
HHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
KEFKSILKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
LNTPPEYSALAIKRLAEITGRAYVPAEDLIEATSDCGAYVMLHGAIKRMAMKMSKICNDL
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTALKEINLPEMQAGSSIMPAKVNPVIPEVVNQTCFKVFGNDVTITFAAEAGQ
HHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCC
LQLNVMEPVIGQAMFESLSLMEKACISLREKCVEGITANPEICMNHVLNSIGIVTYLNPF
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGHHEGDIVGKICAQTGKNVREVVLERGLLTAEQLDEILSIENLMNPQYKAKRYTREATV
HCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]