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The map label for this gene is rfaH [H]

Identifier: 145300265

GI number: 145300265

Start: 3656343

End: 3656840

Strand: Direct

Name: rfaH [H]

Synonym: ASA_3381

Alternate gene names: 145300265

Gene position: 3656343-3656840 (Clockwise)

Preceding gene: 145300264

Following gene: 145300270

Centisome position: 77.75

GC content: 57.43

Gene sequence:

>498_bases
ATGAAAAAATGGTATCTGGCCTACTGCAAGCCAAAGGAAGAGGCCCGCGCACGCGCCCATCTCGAAGCCCAGGGGATTGA
GTCCTATTACCCCATGGTCGAGATCGAGAAGCTGCGACGGGGCAAGCGTGTCCCTGTGCGGGAACCCATGTTTCCCAACT
ATCTGTTTATCTATGTGGATCTGGAGGAGGTCACGCCGGTCACCCTGAAGTCGACCCGTGGCATCAGCCGCATCATCCAC
TTCGGCAAGGAGTGGACCGCCATCAGCAGCCAGCTGGTTTATCAGTTGATGAGCCGTGACGACAGCGACGAGGCGAGAGC
CAGCTACGCCAATCTGCCAACCCAGGGCGACAAGGTATTGATCGAATCGGGCCCATTGGCCGGATTCGAGGCGATTTATC
TGGAACCAGATGGAGAGAAACGCGCCATCCTGCTGGTCAGCCTGCTCAACCAGGAGACCTCCAGCAGCTTCGACAATCAG
GCTTTTCGCCGCCTGTAA

Upstream 100 bases:

>100_bases
GGTAATCACTCACTCAGCAAAAAAGGCCACGCCAAGCGTGGCCTTTTTTATTGCCGCGGTTATCATAACCGGGAGCACGG
CAACACGAACGTAACAGAAC

Downstream 100 bases:

>100_bases
TGACCCTCATCAAGCAGGCGTTCATGACAAGCAACGCCTCATTCCTCAAGCAGCTTCGACTATCAGGCTGCCTGCCGCCT
GTACCAATGCTGAGGCGGGC

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKKWYLAYCKPKEEARARAHLEAQGIESYYPMVEIEKLRRGKRVPVREPMFPNYLFIYVDLEEVTPVTLKSTRGISRIIH
FGKEWTAISSQLVYQLMSRDDSDEARASYANLPTQGDKVLIESGPLAGFEAIYLEPDGEKRAILLVSLLNQETSSSFDNQ
AFRRL

Sequences:

>Translated_165_residues
MKKWYLAYCKPKEEARARAHLEAQGIESYYPMVEIEKLRRGKRVPVREPMFPNYLFIYVDLEEVTPVTLKSTRGISRIIH
FGKEWTAISSQLVYQLMSRDDSDEARASYANLPTQGDKVLIESGPLAGFEAIYLEPDGEKRAILLVSLLNQETSSSFDNQ
AFRRL
>Mature_165_residues
MKKWYLAYCKPKEEARARAHLEAQGIESYYPMVEIEKLRRGKRVPVREPMFPNYLFIYVDLEEVTPVTLKSTRGISRIIH
FGKEWTAISSQLVYQLMSRDDSDEARASYANLPTQGDKVLIESGPLAGFEAIYLEPDGEKRAILLVSLLNQETSSSFDNQ
AFRRL

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=168, Percent_Identity=42.8571428571429, Blast_Score=147, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 18994; Mature: 18994

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWYLAYCKPKEEARARAHLEAQGIESYYPMVEIEKLRRGKRVPVREPMFPNYLFIYVD
CCCEEEEECCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
LEEVTPVTLKSTRGISRIIHFGKEWTAISSQLVYQLMSRDDSDEARASYANLPTQGDKVL
HHHCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEE
IESGPLAGFEAIYLEPDGEKRAILLVSLLNQETSSSFDNQAFRRL
EECCCCCCEEEEEECCCCCCCEEEEEEHHCCHHCCCHHHHHHHCC
>Mature Secondary Structure
MKKWYLAYCKPKEEARARAHLEAQGIESYYPMVEIEKLRRGKRVPVREPMFPNYLFIYVD
CCCEEEEECCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
LEEVTPVTLKSTRGISRIIHFGKEWTAISSQLVYQLMSRDDSDEARASYANLPTQGDKVL
HHHCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEE
IESGPLAGFEAIYLEPDGEKRAILLVSLLNQETSSSFDNQAFRRL
EECCCCCCEEEEEECCCCCCCEEEEEEHHCCHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]