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The map label for this gene is ada [H]

Identifier: 145300151

GI number: 145300151

Start: 3519175

End: 3520743

Strand: Direct

Name: ada [H]

Synonym: ASA_3254

Alternate gene names: 145300151

Gene position: 3519175-3520743 (Clockwise)

Preceding gene: 145300149

Following gene: 145300152

Centisome position: 74.84

GC content: 65.84

Gene sequence:

>1569_bases
ATGACCCCACAGCCCGTCAGTGACAACCCTCAGGATGAAGGGATCCCGAGCCTCAGCCGCGAACAGTGCCATGCAGCCCG
CCTCGCCCGCGATGCGCGCTTCGATGGCCGTTTCTTTACCGGCGTGCTCTCCACCGGCATCTACTGCCGCCCCGTCTGCC
CCGCTCGGCCACCGCACGAGCACAACGTGCGCTACTTCCGAAGCGCAGCCGCCGCCGAGCAGCATGGTCTGCGCCCATGT
CTGCGCTGTCGCCCCGAGCTGGCCCCTTCCGCCAGCGGCGATCTGCCGCCCGGACTCGCCCGTTTGCTGGCGCGGATCGA
ACGTGGCGAGCTGGCAGACACAACGCTCGCAATGCTCGCGCAGCAGGCCGGGATCACGGAGCGCACGCTGCGCCGCCAGT
TCGAACAGCACCTTGGTGCCTCGCCAAAACAGGTGGAGCAGACGCGCCGCCTGCTGCTCGCGAAACGGCTGCTGACCGAA
ACTGCACTGCCCATCACGGATATCGCATTTGCGGCAGGCTTTGCGAGCATCCGCCGCTTCAACGATGCCTGGAGCCAGGC
GTATGGCCTCGCGCCCAGAGCCCTGCGACAACAGCAAGGGAATGAGGGGGCGGCGGAAGGTGGCGCTGCACTCACGCTGC
AATTGCCGTATCGCCCGCCCTACGATGTGGCGGCCATGCTGGCGTTCTATCGGCTACGGGCCATCCCCGGGCTGGAGCGG
GTTGATGGAAATGTGTATGAACGGCGCCACAGGGTCGGCGATCAGTCTGGATGGATCCGCATCGAGCAGGGCAAGGGCCA
TAGCATCAGGCTCACAGTGCATGATCTGCCGCCCGCTGCACTGCCTGATCTCTTGTATCGCGTGCGCCGCATGTGGGATC
TCGATGCCGACATGCAGCGCATCGGCGAGCGGCTCGGCCAGGATCCGCTGCTGGCCCGCTTGCAGTCACGCTGGCCCGGG
GTGCGACTGCCCGCCGGTTGGGATGAGTACGAGGTGATGTTGCGCGCCATTGTTGGCCAGCAGGTATCGGTCAAGGGCGC
CATCACCATCATGGGTCGGCTGCTGGCGCGTACCGAGGCGCAATTCGGCGTGGCCCAACTGCCCACACCAGCGCAGCTGT
GTGCACTGGATCTCGACGGCATCGGCATGCCCGGCAGTCGCATCCGCACGCTGCAAGGGCTCGCCGCTGCGCTTGCAAGC
GGTACGCTTTCCCTGAACACGGCCAGCGACGAACAATTATTGGCCTTGCCGGGCATAGGCCCCTGGACGGTCGCCTACTG
GCGGCTACGCTGCGGGCAGGATCCGGACGCCTTCCCCGCCTCCGATCTGGTGCTGCAAAAGGCGCTGGGGGGCGGCGACA
AGCTGCCAGTGAAGGAGGTGCTGGTACAAAGCGAAGCATGGCAACCCTGGCGCGGCTATGCCGCGAGCTGGCTGTGGCAC
GCCATGAGCGAACAACCGGCACTGCTCATCAAGCAGCCATCGACCCCCACTGCGAGCCTGGTTTCACCCACAACGGGGAG
CAATCCGCCCCAACGCTATGACGAGGAGATAACCCATGATCCGCTATGA

Upstream 100 bases:

>100_bases
CCGACGACAATTTTATTTCAATTCCTGTCCGTTTCTGGCTAGTCACTGCGCCCACTCCCCCTTACTCTCAGCCATACACC
CAATGCAGAGCTTGCATCCC

Downstream 100 bases:

>100_bases
CGTGTTGCCAACAGACTGCGGCGAACTGCTGGTCGCCATCAACGCCCGCGGCCTGGTGCACGTGGACTTTGTGGCTGGCC
TGCGGGCACTGCCCGATATG

Product: DNA methylation and regulatory protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MTPQPVSDNPQDEGIPSLSREQCHAARLARDARFDGRFFTGVLSTGIYCRPVCPARPPHEHNVRYFRSAAAAEQHGLRPC
LRCRPELAPSASGDLPPGLARLLARIERGELADTTLAMLAQQAGITERTLRRQFEQHLGASPKQVEQTRRLLLAKRLLTE
TALPITDIAFAAGFASIRRFNDAWSQAYGLAPRALRQQQGNEGAAEGGAALTLQLPYRPPYDVAAMLAFYRLRAIPGLER
VDGNVYERRHRVGDQSGWIRIEQGKGHSIRLTVHDLPPAALPDLLYRVRRMWDLDADMQRIGERLGQDPLLARLQSRWPG
VRLPAGWDEYEVMLRAIVGQQVSVKGAITIMGRLLARTEAQFGVAQLPTPAQLCALDLDGIGMPGSRIRTLQGLAAALAS
GTLSLNTASDEQLLALPGIGPWTVAYWRLRCGQDPDAFPASDLVLQKALGGGDKLPVKEVLVQSEAWQPWRGYAASWLWH
AMSEQPALLIKQPSTPTASLVSPTTGSNPPQRYDEEITHDPL

Sequences:

>Translated_522_residues
MTPQPVSDNPQDEGIPSLSREQCHAARLARDARFDGRFFTGVLSTGIYCRPVCPARPPHEHNVRYFRSAAAAEQHGLRPC
LRCRPELAPSASGDLPPGLARLLARIERGELADTTLAMLAQQAGITERTLRRQFEQHLGASPKQVEQTRRLLLAKRLLTE
TALPITDIAFAAGFASIRRFNDAWSQAYGLAPRALRQQQGNEGAAEGGAALTLQLPYRPPYDVAAMLAFYRLRAIPGLER
VDGNVYERRHRVGDQSGWIRIEQGKGHSIRLTVHDLPPAALPDLLYRVRRMWDLDADMQRIGERLGQDPLLARLQSRWPG
VRLPAGWDEYEVMLRAIVGQQVSVKGAITIMGRLLARTEAQFGVAQLPTPAQLCALDLDGIGMPGSRIRTLQGLAAALAS
GTLSLNTASDEQLLALPGIGPWTVAYWRLRCGQDPDAFPASDLVLQKALGGGDKLPVKEVLVQSEAWQPWRGYAASWLWH
AMSEQPALLIKQPSTPTASLVSPTTGSNPPQRYDEEITHDPL
>Mature_521_residues
TPQPVSDNPQDEGIPSLSREQCHAARLARDARFDGRFFTGVLSTGIYCRPVCPARPPHEHNVRYFRSAAAAEQHGLRPCL
RCRPELAPSASGDLPPGLARLLARIERGELADTTLAMLAQQAGITERTLRRQFEQHLGASPKQVEQTRRLLLAKRLLTET
ALPITDIAFAAGFASIRRFNDAWSQAYGLAPRALRQQQGNEGAAEGGAALTLQLPYRPPYDVAAMLAFYRLRAIPGLERV
DGNVYERRHRVGDQSGWIRIEQGKGHSIRLTVHDLPPAALPDLLYRVRRMWDLDADMQRIGERLGQDPLLARLQSRWPGV
RLPAGWDEYEVMLRAIVGQQVSVKGAITIMGRLLARTEAQFGVAQLPTPAQLCALDLDGIGMPGSRIRTLQGLAAALASG
TLSLNTASDEQLLALPGIGPWTVAYWRLRCGQDPDAFPASDLVLQKALGGGDKLPVKEVLVQSEAWQPWRGYAASWLWHA
MSEQPALLIKQPSTPTASLVSPTTGSNPPQRYDEEITHDPL

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=290, Percent_Identity=32.7586206896552, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788542, Length=174, Percent_Identity=32.7586206896552, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 57413; Mature: 57282

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQPVSDNPQDEGIPSLSREQCHAARLARDARFDGRFFTGVLSTGIYCRPVCPARPPHE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCCCCC
HNVRYFRSAAAAEQHGLRPCLRCRPELAPSASGDLPPGLARLLARIERGELADTTLAMLA
CCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
QQAGITERTLRRQFEQHLGASPKQVEQTRRLLLAKRLLTETALPITDIAFAAGFASIRRF
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
NDAWSQAYGLAPRALRQQQGNEGAAEGGAALTLQLPYRPPYDVAAMLAFYRLRAIPGLER
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHH
VDGNVYERRHRVGDQSGWIRIEQGKGHSIRLTVHDLPPAALPDLLYRVRRMWDLDADMQR
HCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHH
IGERLGQDPLLARLQSRWPGVRLPAGWDEYEVMLRAIVGQQVSVKGAITIMGRLLARTEA
HHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QFGVAQLPTPAQLCALDLDGIGMPGSRIRTLQGLAAALASGTLSLNTASDEQLLALPGIG
HCCHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCC
PWTVAYWRLRCGQDPDAFPASDLVLQKALGGGDKLPVKEVLVQSEAWQPWRGYAASWLWH
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
AMSEQPALLIKQPSTPTASLVSPTTGSNPPQRYDEEITHDPL
HHCCCCEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
TPQPVSDNPQDEGIPSLSREQCHAARLARDARFDGRFFTGVLSTGIYCRPVCPARPPHE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCCCCC
HNVRYFRSAAAAEQHGLRPCLRCRPELAPSASGDLPPGLARLLARIERGELADTTLAMLA
CCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
QQAGITERTLRRQFEQHLGASPKQVEQTRRLLLAKRLLTETALPITDIAFAAGFASIRRF
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
NDAWSQAYGLAPRALRQQQGNEGAAEGGAALTLQLPYRPPYDVAAMLAFYRLRAIPGLER
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHH
VDGNVYERRHRVGDQSGWIRIEQGKGHSIRLTVHDLPPAALPDLLYRVRRMWDLDADMQR
HCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHH
IGERLGQDPLLARLQSRWPGVRLPAGWDEYEVMLRAIVGQQVSVKGAITIMGRLLARTEA
HHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QFGVAQLPTPAQLCALDLDGIGMPGSRIRTLQGLAAALASGTLSLNTASDEQLLALPGIG
HCCHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCC
PWTVAYWRLRCGQDPDAFPASDLVLQKALGGGDKLPVKEVLVQSEAWQPWRGYAASWLWH
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
AMSEQPALLIKQPSTPTASLVSPTTGSNPPQRYDEEITHDPL
HHCCCCEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]