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The map label for this gene is 145300149

Identifier: 145300149

GI number: 145300149

Start: 3517037

End: 3518515

Strand: Direct

Name: 145300149

Synonym: ASA_3252

Alternate gene names: NA

Gene position: 3517037-3518515 (Clockwise)

Preceding gene: 145300148

Following gene: 145300151

Centisome position: 74.79

GC content: 63.22

Gene sequence:

>1479_bases
ATGTTATATCAAAAACTTGAACAACATCAGCAACAGCTGTACCGGCTCTCCCACCTCAGCGCCATCTGCGGCTGGGATCA
GGCCGCCATGATGCCGGAGGGCGGCAACGAGGCACGCGCCGAGGCCATGGCCGAACTCGGCGTGCTGATCCACCACAAAC
GCACAGCCCCCGAGCTTGGCGACTGGATAGCCAAGGCCGAAAGCGAGCCGCTGGATAACGCTCAGCGCGCCAATCTCGCC
GAGATAAAACGCCACTGGCAGGATGCGAGCCTGCTGCCCACGGATCTGGTCGAGGCCTTATCCCTCGCGGGCAGCAAGTG
CGAGCACGCCTGGCGCAGCCAACGCAAAAACAACGATTGGGCAGGCTTCGCCCCCAATCTGAAGGAAGTGGTGGCCCTGT
CGCGGGAAGTGGCGCGCCTTCGCGCCGAGGCGCTTGATGTACGCCCCTACGACGCCATGCTGGCGCTGTACGAACCCGGC
ATGACGAGCGCGCGGCTGGATCAGATCTTCGGCGATCTCACTGGCTGGCTGCCCGGCCTGATCCAGCAAGTGAGTGAACA
GCAGAAATATGAATCCATCTTGATCCCGCAAGGTCCCTTCCCCATCGCAAATCAGCAGGCGCTCGGCCAGGAGGTAATGG
GGCTGCTCGGCTTTGATTTCGCCCACGGTCGGCTCGATGTGAGCAGCCACCCCTTCTGTGGCGGCGTGCCGACCGACGTG
CGCATCACCACCCGCTACAACGAGCAGGAATTCGTCTCCAGCCTGATGGGCATAGTGCATGAAACCGGCCACGCCCGTTA
CGAGCAACGGCTGCCGCGCCAGCTGCTGGGCCAGCCGGTGGCCGAAGCGCGATCCATGGGCATTCACGAAAGCCAGAGCC
TGTTCTGCGAAATGCAGCTCGGCCACGATGCGGCCTTCCTGGCGCTGCTGGCACCGCACATTCGCGCCCACTTTGGCGAG
CAGGCGGCGTTTGCGCCGGCGAACCTGGTGAAGCTCTACAACCGGGTCGAGCCCGGCTTTATCCGGGTCGATGCGGACGA
GGTGACCTACCCGGCTCACGTGATCCTGCGCTACGAGCTGGAGCGTGACCTTATGGAAGGGCGCATCGAGGTGGCGGATC
TGCCCGAGCTGTGGGATGCCAAGATGCAGCAGTGGCTCGGCCTCTCCACCCAGGGCAACTACCAGAATGGCTGCATGCAG
GACATTCACTGGACAGACGGCTCGTTTGGCTACTTCCCGAGCTACACGCTCGGCGCCATGTATGCGGCGCAGCTGCGTTT
TGCCCTCGAGAAAGAGCTGGGGAACATGAGCAGCGTGATCGAGGCGGGCCGCCTGCCGGAGGTGCTCGCCTGGCTTGGTC
GCAACATCTGGTCACAGGGCAGTATGCACAGCACGGATGAGCTCATTCGCCGTGCGACTGGCGAGGCGCTGAACCCGCGC
TGGCTGCGCCAGCATCTGGAGCAGCGCTATCTGAAATAA

Upstream 100 bases:

>100_bases
CCAGGGTGGTAAACGCGCGATGGCAGTGTTAGCATCGATCGGCCCCGATCATGCCAGGTACGGGGTGCATACCATCAGGG
ACGATAACAAGGACAATCCC

Downstream 100 bases:

>100_bases
CGGATGCGGCGACAGCGAATGAAGAGGGCAACGGGGCAGCGAGACTGTCCCGTTTTTATGGGCAATCCATCACAGCGGGT
CGGGGGTGGTGAGAAGCTCG

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLYQKLEQHQQQLYRLSHLSAICGWDQAAMMPEGGNEARAEAMAELGVLIHHKRTAPELGDWIAKAESEPLDNAQRANLA
EIKRHWQDASLLPTDLVEALSLAGSKCEHAWRSQRKNNDWAGFAPNLKEVVALSREVARLRAEALDVRPYDAMLALYEPG
MTSARLDQIFGDLTGWLPGLIQQVSEQQKYESILIPQGPFPIANQQALGQEVMGLLGFDFAHGRLDVSSHPFCGGVPTDV
RITTRYNEQEFVSSLMGIVHETGHARYEQRLPRQLLGQPVAEARSMGIHESQSLFCEMQLGHDAAFLALLAPHIRAHFGE
QAAFAPANLVKLYNRVEPGFIRVDADEVTYPAHVILRYELERDLMEGRIEVADLPELWDAKMQQWLGLSTQGNYQNGCMQ
DIHWTDGSFGYFPSYTLGAMYAAQLRFALEKELGNMSSVIEAGRLPEVLAWLGRNIWSQGSMHSTDELIRRATGEALNPR
WLRQHLEQRYLK

Sequences:

>Translated_492_residues
MLYQKLEQHQQQLYRLSHLSAICGWDQAAMMPEGGNEARAEAMAELGVLIHHKRTAPELGDWIAKAESEPLDNAQRANLA
EIKRHWQDASLLPTDLVEALSLAGSKCEHAWRSQRKNNDWAGFAPNLKEVVALSREVARLRAEALDVRPYDAMLALYEPG
MTSARLDQIFGDLTGWLPGLIQQVSEQQKYESILIPQGPFPIANQQALGQEVMGLLGFDFAHGRLDVSSHPFCGGVPTDV
RITTRYNEQEFVSSLMGIVHETGHARYEQRLPRQLLGQPVAEARSMGIHESQSLFCEMQLGHDAAFLALLAPHIRAHFGE
QAAFAPANLVKLYNRVEPGFIRVDADEVTYPAHVILRYELERDLMEGRIEVADLPELWDAKMQQWLGLSTQGNYQNGCMQ
DIHWTDGSFGYFPSYTLGAMYAAQLRFALEKELGNMSSVIEAGRLPEVLAWLGRNIWSQGSMHSTDELIRRATGEALNPR
WLRQHLEQRYLK
>Mature_492_residues
MLYQKLEQHQQQLYRLSHLSAICGWDQAAMMPEGGNEARAEAMAELGVLIHHKRTAPELGDWIAKAESEPLDNAQRANLA
EIKRHWQDASLLPTDLVEALSLAGSKCEHAWRSQRKNNDWAGFAPNLKEVVALSREVARLRAEALDVRPYDAMLALYEPG
MTSARLDQIFGDLTGWLPGLIQQVSEQQKYESILIPQGPFPIANQQALGQEVMGLLGFDFAHGRLDVSSHPFCGGVPTDV
RITTRYNEQEFVSSLMGIVHETGHARYEQRLPRQLLGQPVAEARSMGIHESQSLFCEMQLGHDAAFLALLAPHIRAHFGE
QAAFAPANLVKLYNRVEPGFIRVDADEVTYPAHVILRYELERDLMEGRIEVADLPELWDAKMQQWLGLSTQGNYQNGCMQ
DIHWTDGSFGYFPSYTLGAMYAAQLRFALEKELGNMSSVIEAGRLPEVLAWLGRNIWSQGSMHSTDELIRRATGEALNPR
WLRQHLEQRYLK

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 55474; Mature: 55474

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQKLEQHQQQLYRLSHLSAICGWDQAAMMPEGGNEARAEAMAELGVLIHHKRTAPELG
CCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
DWIAKAESEPLDNAQRANLAEIKRHWQDASLLPTDLVEALSLAGSKCEHAWRSQRKNNDW
HHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
AGFAPNLKEVVALSREVARLRAEALDVRPYDAMLALYEPGMTSARLDQIFGDLTGWLPGL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IQQVSEQQKYESILIPQGPFPIANQQALGQEVMGLLGFDFAHGRLDVSSHPFCGGVPTDV
HHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCE
RITTRYNEQEFVSSLMGIVHETGHARYEQRLPRQLLGQPVAEARSMGIHESQSLFCEMQL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCHHEEEEEC
GHDAAFLALLAPHIRAHFGEQAAFAPANLVKLYNRVEPGFIRVDADEVTYPAHVILRYEL
CCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHH
ERDLMEGRIEVADLPELWDAKMQQWLGLSTQGNYQNGCMQDIHWTDGSFGYFPSYTLGAM
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
YAAQLRFALEKELGNMSSVIEAGRLPEVLAWLGRNIWSQGSMHSTDELIRRATGEALNPR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHH
WLRQHLEQRYLK
HHHHHHHHHCCC
>Mature Secondary Structure
MLYQKLEQHQQQLYRLSHLSAICGWDQAAMMPEGGNEARAEAMAELGVLIHHKRTAPELG
CCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
DWIAKAESEPLDNAQRANLAEIKRHWQDASLLPTDLVEALSLAGSKCEHAWRSQRKNNDW
HHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
AGFAPNLKEVVALSREVARLRAEALDVRPYDAMLALYEPGMTSARLDQIFGDLTGWLPGL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IQQVSEQQKYESILIPQGPFPIANQQALGQEVMGLLGFDFAHGRLDVSSHPFCGGVPTDV
HHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCE
RITTRYNEQEFVSSLMGIVHETGHARYEQRLPRQLLGQPVAEARSMGIHESQSLFCEMQL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCHHEEEEEC
GHDAAFLALLAPHIRAHFGEQAAFAPANLVKLYNRVEPGFIRVDADEVTYPAHVILRYEL
CCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHH
ERDLMEGRIEVADLPELWDAKMQQWLGLSTQGNYQNGCMQDIHWTDGSFGYFPSYTLGAM
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
YAAQLRFALEKELGNMSSVIEAGRLPEVLAWLGRNIWSQGSMHSTDELIRRATGEALNPR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHH
WLRQHLEQRYLK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]