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The map label for this gene is ogt [H]

Identifier: 145300152

GI number: 145300152

Start: 3520730

End: 3521224

Strand: Direct

Name: ogt [H]

Synonym: ASA_3255

Alternate gene names: 145300152

Gene position: 3520730-3521224 (Clockwise)

Preceding gene: 145300151

Following gene: 145300158

Centisome position: 74.87

GC content: 63.64

Gene sequence:

>495_bases
ATGATCCGCTATGACGTGTTGCCAACAGACTGCGGCGAACTGCTGGTCGCCATCAACGCCCGCGGCCTGGTGCACGTGGA
CTTTGTGGCTGGCCTGCGGGCACTGCCCGATATGAGCGGCTGGCAACAAGACAGTGAAGCGCTCGCCCCCTATCTTGCCG
AATTTGCCGCCTATTTCGCCGGGCGTCTGCAGCGCTTTACCCTGCCGCTCGCCGCCCACGGCACCGCCTTTCAGCAGGCG
GTATGGCAGGCACTGTGCGACATTCCCTATGGCGAGACCCGCAGTTATGGCGATATCGCCCGCGCCATCGGCAAACCGGC
GGCGGTACGGGCAGTGGGCGCGGCGAACGGTCGCAATCCGCTCTCGATCATAGTGCCGTGCCATCGCGTGATAGGCCAAA
ACGGCAGCCTGACCGGTTATGCGGGCGGGCTGCCCATCAAGCAGCAGTTGCTGGCGCTGGAATGTGATCAGTCACGTTCA
GCCAACCAGCCATGA

Upstream 100 bases:

>100_bases
CCGGCACTGCTCATCAAGCAGCCATCGACCCCCACTGCGAGCCTGGTTTCACCCACAACGGGGAGCAATCCGCCCCAACG
CTATGACGAGGAGATAACCC

Downstream 100 bases:

>100_bases
AAACCGAGCGCCTGAAAATAGCGCCAGCCAGTGCCAAACCCGGCAGCCCCCAGCAGTTGCCGGGTTTGGTCCTCATTCAA
GGGGAAAAAGTCCCGATCCA

Product: methylated-DNA-[protein]-cysteineS- methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MIRYDVLPTDCGELLVAINARGLVHVDFVAGLRALPDMSGWQQDSEALAPYLAEFAAYFAGRLQRFTLPLAAHGTAFQQA
VWQALCDIPYGETRSYGDIARAIGKPAAVRAVGAANGRNPLSIIVPCHRVIGQNGSLTGYAGGLPIKQQLLALECDQSRS
ANQP

Sequences:

>Translated_164_residues
MIRYDVLPTDCGELLVAINARGLVHVDFVAGLRALPDMSGWQQDSEALAPYLAEFAAYFAGRLQRFTLPLAAHGTAFQQA
VWQALCDIPYGETRSYGDIARAIGKPAAVRAVGAANGRNPLSIIVPCHRVIGQNGSLTGYAGGLPIKQQLLALECDQSRS
ANQP
>Mature_164_residues
MIRYDVLPTDCGELLVAINARGLVHVDFVAGLRALPDMSGWQQDSEALAPYLAEFAAYFAGRLQRFTLPLAAHGTAFQQA
VWQALCDIPYGETRSYGDIARAIGKPAAVRAVGAANGRNPLSIIVPCHRVIGQNGSLTGYAGGLPIKQQLLALECDQSRS
ANQP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=117, Percent_Identity=39.3162393162393, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=55.8823529411765, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1788542, Length=162, Percent_Identity=41.9753086419753, Blast_Score=108, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=56.4705882352941, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=58.75, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=49.4252873563218, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=33.7142857142857, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17535; Mature: 17535

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYDVLPTDCGELLVAINARGLVHVDFVAGLRALPDMSGWQQDSEALAPYLAEFAAYFA
CEEECCCCCCHHHEEEHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
GRLQRFTLPLAAHGTAFQQAVWQALCDIPYGETRSYGDIARAIGKPAAVRAVGAANGRNP
HHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHEEEECCCCCCCC
LSIIVPCHRVIGQNGSLTGYAGGLPIKQQLLALECDQSRSANQP
EEEEEEHHHHHCCCCCCCEECCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIRYDVLPTDCGELLVAINARGLVHVDFVAGLRALPDMSGWQQDSEALAPYLAEFAAYFA
CEEECCCCCCHHHEEEHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
GRLQRFTLPLAAHGTAFQQAVWQALCDIPYGETRSYGDIARAIGKPAAVRAVGAANGRNP
HHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHEEEECCCCCCCC
LSIIVPCHRVIGQNGSLTGYAGGLPIKQQLLALECDQSRSANQP
EEEEEEHHHHHCCCCCCCEECCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA